Re: [Histonet] Histotech needed immediately

2019-03-05 Thread Marsha Price via Histonet 
A dermpath lab in Glenwood, Colorado is in immediate need for a high complexity 
testing qualified histology tech. If you are able to fill in for them on an 
emergency basis, please respond

Thank you,
Marsha Price

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From: histonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, March 5, 2019 12:19 PM
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Subject: Histonet Digest, Vol 184, Issue 4

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Today's Topics:

1. Pathology QA Reports (Mike Pence)
2. Re: Roche "bashing" (Terri Braud)
3. Histotechnology Professionals Day is Sunday March 10th Happy
Histo Day!! (Pam Barker)
4. HPV ISH (Jimenez, Roberto)
5. Re: Histonet Digest, Vol 184, Issue 1 (Mark Tarango)
6. Re: Histonet Digest, Vol 184, Issue 1 (Joseph Saby)


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Message: 1
Date: Mon, 4 Mar 2019 20:51:32 +
From: Mike Pence 
To: "histonet@lists.utsouthwestern.edu"
,
"histonet-boun...@lists.utsouthwestern.edu"

Subject: [Histonet] Pathology QA Reports
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How long are we required to keep routine QA reports?

Michael S. Pence | Supervisor of Laboratory Services
Great River Health Systems
1221 S. Gear Ave. | West Burlington, IA 52655
Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
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Message: 2
Date: Tue, 5 Mar 2019 15:23:26 +
From: "Terri Braud" 
To: "'histonet@lists.utsouthwestern.edu'"

Cc: "'kelley.jor...@roche.com'" 
Subject: Re: [Histonet] Roche "bashing"
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local>

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Such a wonderful insight into the thought process of Roche. Accused of bashing 
when I am telling the truth about fai

Re: [Histonet] Florida State Licensure

2016-05-11 Thread Marsha Price via Histonet 
Can someone provide the link for the application to obtain HT Florida State 
Licensure

Sent from my iPhone

> On May 10, 2016, at 12:00 PM,  
>  wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
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> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. IHC Wet Workshop announcement (Ihc Workshop)
>   2. Re: PAS Stain (Seeley, Heather)
>   3. Re: Tracking surgical specimens (Jamieson Anderson)
>   4. Tips on staining PLA2R assay on Leica Bond (Eddie Martin)
>   5. Re: PAS Stain (victor_tob...@comcast.net)
>   6. P16 (Charles Riley)
>   7. Cytology/Histology Staining Question (Mullen, Mary)
>   8. Re: Cytology/Histology Staining Question (Rene J Buesa)
>   9. ASCP Certified Pathologist Assistant Job Opening- (Melissa Owens)
>  10. Melanin Bleach (Heckford, Karen - SMMC-SF)
>  11. Re: Melanin Bleach (Jeffrey Robinson)
>  12. Re: Melanin Bleach (Rene J Buesa)
> 
> 
> --
> 
> Message: 1
> Date: Mon, 9 May 2016 10:16:39 -0700
> From: Ihc Workshop 
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: [Histonet] IHC Wet Workshop announcement
> Message-ID: <3fac1e78-7c1b-4cbc-9a99-fda3f2baf...@gmail.com>
> Content-Type: text/plain; charset=us-ascii
> 
> June 23 & 24,  2016.  San Francisco Bay Area 
> This IHC  lab course is aimed at hands-on training of attendees in all 
> aspects of IHC staining of human, mouse and animal tissues with varied 
> antibodies. This is a small group workshop and is taught in a lab.Lots of 
> troubleshooting,  only few spots left.  
> To get more detail contact.  Maria at:   ihcworks...@gmail.com 
> 
> 
> 
> 
> --
> 
> Message: 2
> Date: Mon, 9 May 2016 17:35:02 +
> From: "Seeley, Heather" 
> To: Bob Richmond ,
>"Histonet@lists.utsouthwestern.edu"
>
> Subject: Re: [Histonet] PAS Stain
> Message-ID:
><9dfe334e776e734d9d231ef60cce93c57e26d...@tenhdcthmb10-31.tenethealth.net>
>
> Content-Type: text/plain; charset="us-ascii"
> 
> We have one girl that always spits on our slide! :) works great!
> 
> HEATHER SEELEY, HT(ASCP)
> Histotech
> 803-985-4676 OFFICE
> 803-327-7598 FAX
> 
> 
> 
> From: Bob Richmond [rsrichm...@gmail.com]
> Sent: Thursday, May 05, 2016 2:10 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] PAS Stain
> 
> Amylase (diastase) for the PAS stain queries:
> 
> Whatever happened to spitting on the slide (30 min at room temperature)?
> John Kiernan advises "thinking of lemons and drooling into a small beaker"
> though I'd advise chewing on a rubber band for a few seconds.
> 
> He notes that alpha amylase is preferred. I'd go with the cheapest one in
> the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
> offers a heat-stable alpha amylase.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> 
> 
> --
> 
> Message: 3
> Date: Mon, 9 May 2016 17:41:09 + (UTC)
> From: Jamieson Anderson 
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Tracking surgical specimens
> Message-ID:
><537327fdd6ba9f6c.0b786cd3-7fd7-4574-b1dc-3d754eacb...@mail.outlook.com>
>
> Content-Type: text/plain; charset=us-ascii
> 
> Hi Lynne,
> In our lab we have a log book at Accessioning that each 
> physician/nurse/porter/MOA signs when dropping off specimens. We also 
> encourage them to drop off a QC sheet with a patient label for each specimen 
> that is dropped off. We check these to ensure we have received each specimen, 
> and we date/time stamp them and keep them on record in case we need to refer 
> back to them in the future (ie. In case a clinician claims they dropped off a 
> specimen we can prove they did not).
> Jamieson AndersonTechnical Coordinator - Anatomic PathologySt. Paul's 
> HospitalLower Mainland Pathology & Laboratory Medicine
> 
>_
> 
> Date: Mon, 9 May 2016 14:49:56 +
> From: "Bell, Lynne" 
> To: "Histonet (histonet@lists.utsouthwestern.edu)"
>
> Subject: [Histonet] Tracking surgical specimens brought to the lab
> Message-ID:
>
>

Re: [Histonet] Histonet Digest, Vol 150, Issue 10

2016-05-07 Thread Marsha Price via Histonet 
I know this subject has been discussed on here before, but can someone direct 
me to the correct link for the application to get your Florida HT application? 
Thank You.
Marsha Price

Sent from my iPhone

> On May 7, 2016, at 12:00 PM, <histonet-requ...@lists.utsouthwestern.edu> 
> <histonet-requ...@lists.utsouthwestern.edu> wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
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> or, via email, send a message with subject or body 'help' to
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> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Looking for Part Time Position in Oklahoma
>  (Adesupo, Adesuyi (Banjo))
>   2. Re: Automated IHC instrument (Eddie Martin)
>   3. Leica printer (Ginny Achstetter)
>   4. Histology supervisor position - Children's Minnesota
>  (Megan Dishop)
>   5. Re: picric acid (E. Wayne Johnson)
> 
> 
> --
> 
> Message: 1
> Date: Fri, 6 May 2016 12:08:19 -0500
> From: "Adesupo, Adesuyi (Banjo)" <abades...@nrh-ok.com>
> To: "'histonet@lists.utsouthwestern.edu'"
><histonet@lists.utsouthwestern.edu>
> Subject: [Histonet] Looking for Part Time Position in Oklahoma
> Message-ID:
><04ee4f75bb5fb246adb68d69b7460443a5177b1...@mail.nrhnt.nrh-ok.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> 
>  Hi,
>  I am looking for a part time histo position in Oklahoma City, OK.
> 
>  Thanks,
> 
> 
>   Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
> 
>   Histology Supervisor
> 
>   Norman Regional Health System,
> 
>   Norman, OK 73071.
> 
>   Cell: 405-973-6363
> 
>   Tel: 405- 307- 1145
>abades...@nrh-ok.com<mailto:abades...@nrh-ok.com>
> 
> ==
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> of the designated recipients named above. If you are not 
> the intended recipient, you are hereby notified that you 
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> review, disclosure, dissemination, distribution, or copying 
> of it or its contents is prohibited. If you have received 
> this communication in error, please notify the sender 
> immediately and destroy all copies of this communication 
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> --
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> Message: 2
> Date: Fri, 6 May 2016 13:58:47 -0500
> From: Eddie Martin <edmarti...@gmail.com>
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Automated IHC instrument
> Message-ID: <069d742f-813a-4a3b-8468-5ddd6b451...@gmail.com>
> Content-Type: text/plain; charset=utf-8
> 
> Hi Valerie,
> 
> Any platform would get the job done well.  They all offer reagent rental 
> agreements too.  Dako and Leica would require the user to be a bit more 
> knowledgeable of IHC protocols than starting a run on a Ventana analyzer.  
> Dako is semi-automated, whereas leica and ventana both have onboard 
> retrieval, and reagent mixing of chromagen.  In terms of the most open 
> system, Dako would be the best analyzer followed by Leica in second place.  
> Ventana does allow 3rd party antibodies to be run on it, but charge you a 
> premium for open containers to run on their analyzer.
> 
> In terms of antibodies: this may be better chosen by your pathologist which 
> may have an impact on which analyzer you will also get, but I can suggest to 
> you my favorites.
> 
> Her2neu - all three vendors provide a good Her2neu clone. Dako?s Her2 
> sometimes doesn?t stain as well, so would be my least favorite of the 3. 
> Ventana?s Her2 is the most widely used.
> 
> ER - all 3 vendors are also good.
> 
> PR - Dako and Novocastra have great PR clones.  Ventana?s PR clone is IVD as 
> Dako and Novocastra Clone but is not ASCO-CAP approved antibody.  Ventana 
> will push to say it is IVD, as Dako, and Novocastra (Leica), which is 
> necessary for billing purposes, but their PR clone isn?t as effective as the 
> other two i mentioned.
> 
> Ki67 - Dako?s MIB-1 clone is like the gold standard for Ki-67.  All three 
> vendor versions work as intended.  Leica removed one of their two clone 
> options last year, so k2 clone for Leica is your onl

Re: [Histonet] Short Term travel Tech Agencies

2015-05-08 Thread Marsha Price 
Does anyone know of an agency that hires Histology Techs for short term 
assignments, less than 3 month assignments?
Marsha Price

Sent from my iPad

 On May 8, 2015, at 12:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
 
 Send Histonet mailing list submissions to
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 When replying, please edit your Subject line so it is more specific
 than Re: Contents of Histonet digest...
 
 
 Today's Topics:
 
   1. Still having Issues with Acetone fixation. (Lewis, Patrick)
   2. Re: Histonet Digest, Vol 138, Issue 9 (Myra Huth)
   3. Final Week to Register for FSH (John Shelley)
   4. Program in Interactive tissue microarray and quantitative
  digital pathology workshop announcement (Linda Margraf)
   5. Billing question (Vickroy, James)
   6. Re: Billing question (Weems, Joyce K.)
   7. HT jobs in Eureka, CA (Jennifer MacDonald)
   8. Re: Billing question (Campbell, Tasha M.)
   9. B-gal positive control (Coffey, Anna (NIH/NCI) [C])
  10. Re: Billing question (della Speranza, Vinnie)
  11. Re: B-gal positive control (koelli...@comcast.net)
  12. Re: Billing question (Weems, Joyce K.)
  13. Friday Trivia Question: Most specimen on a single case
  (Michael Mihalik)
  14. Re: Friday Trivia Question: Most specimen on a single case
  (Stacy McLaughlin)
  15. Re: Friday Trivia Question: Most specimen on a singlecase
  (Marcum, Pamela A)
  16. Re: Friday Trivia Question: Most specimen on a singlecase
  (Cooper, Brian)
  17. Re: Friday Trivia Question: Most specimen on a single case
  (Morken, Timothy)
 
 
 --
 
 Message: 1
 Date: Thu, 7 May 2015 17:01:46 +
 From: Lewis, Patrick patrick.le...@seattlechildrens.org
 To:  (Histonet@lists.utsouthwestern.edu)
Histonet@lists.utsouthwestern.edu,16S-CSF Study
16s-csfst...@seattlechildrens.org
 Subject: [Histonet] Still having Issues with Acetone fixation.
 Message-ID:
3903be18914f4440834f0e620415ffca3cb6d...@ppwexd01d.childrens.sea.kids

 Content-Type: text/plain; charset=us-ascii
 
 Hi everyone,
 
 Sorry to keep posting about this,
 
 But I am still having Acetone issues.
 
 I am doing IHC for Cell surface markers that are lost when fixing with etoh 
 or methanol.
 
 When I fix in 100% acetone my epitopes have great signal.
 
 However,  when I fix in 100% acetone, my tissues all damaged by the acetone 
 beyond all recognition.
 
 I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes.
 
 But, I get good epitope staining if I have some tissue left on the slide.
 
 When I fix in anything else, I lose 90% or more of my epitope staining, but 
 my tissue morphology looks great.
 
 --
 What's the least amount of time I can fix in 100% Acetone for a 5uM section 
 and still have it be fixed?
 
 Is drying after the 100% acetone fixing essential? or Bad for protecting 
 tissue morphology?
 
 --
 I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning.
 
 When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry for 1 
 hour in the fume hood
 
 Should it go straight into buffer? Should it be for less time in the acetone?
 Should the acetone be Room temp or -20C instead of 4C.
 
 If I was diluting the acetone with buffer, (or etoh) then I could see going 
 straight into buffer afterwards, but because I am using 100% I think that 
 going into liquid right after fixing is too much of a change and my tissues 
 go BOOM.
 
 Please help.
 
 Patrick Lewis
 
 
 Patrick Lewis
 Research Associate II Bench
 Seattle Childrens Research Institute
 206-884-1115
 
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 Message: 2
 Date: Thu, 7 May 2015 17:34:33 +
 From: Myra Huth mh...@baymedical.org
 To: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 9
 Message-ID:
6b3280e0cd2c394894743e076dc66c59e...@bmcmsexchmb2.corp.baymedical.org

 Content-Type: text/plain; charset=us-ascii
 
 For some reason histonet is being delivered blank.
 
 -Original Message-
 From: histonet-requ...@lists.utsouthwestern.edu 
 [mailto:histonet-requ...@lists.utsouthwestern.edu]
 Sent: Thursday, May 07, 2015 12:00

[Histonet] 'Help'

2014-12-30 Thread Marsha Price 


Sent from my iPhone

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