[Histonet] Warm formalin
Hello everyone! We have a referring clinician that is concerned about leaving his specimens in an outdoor lockbox in the summer because the formalin will get hot. I don't think that having some specimens in formalin in hot weather would cause any problems but I can't find any references one way or another. Does anyone have any policies regarding this? Thanks so much! Erin Martin, Histology Supervisor UCSF Dermatopathology & Oral Pathology Service Phone: 415-3537248 | Fax: 415-353-7543 CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] New lab set up
Hi Vanessa! It's funny that you asked this question because we were just talking about this a few days ago in my lab. Our tables are low and we all sit. We were discussing that we would like to have higher tables so that we could have the option to stand or sit on a higher chair. Since we are usually embedding for 4 to 5 hours the ability to change positions would be nice. So my suggestion would be a get a higher table so that you could sit or stand depending on your mood! Have a great Wednesday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero Street, RM 230 San Francisco, CA 94115 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From: Vanessa Avalos Sent: Monday, September 16, 2019 1:02 PM To: HISTONET LISTS Subject: [Histonet] New lab set up We will be setting up our lab on the 2nd floor in the next few months and since it's been a while since I have set up a new lab I am looking for any ideas you all have. Our lab is a small one that does derm/H&E only for our 3 locations. I have always stood to section, but am thinking of getting a higher chair and sitting. What do you all think of sitting vs standing, along with counter height suggestions? All input whether it pertains to our lab or not will be appreciated because I am sure there will be something I will forget. Thanks! V. Avalos AD HISTO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain consulting services
Good morning all! We are having a problem with our H&E. My chief pathologist believes that we have day to day variations in intensity as well as variations from slide to slide (even within a rack), and that the hematoxylin gets darker as the week goes on. We use 2 automated Sakura Prisma strainers and now use all pre-made, purchased solutions (to rule out human error in prep). We run A LOT of controls, 4 (different blocks) on each stainer at the beginning of the day before patient cases, another set after all patient cases, and then another set after we rotate the reagents at the end of the day, for a total of 24 per day. Unfortunately the staining on all the controls looks the same and my chief pathologist says that control slides are not useful in the illustration of the problem. So I have two questions from him: 1. Have those using Richard Allan Hematoxylin 7212 noticed this problem? He believes that there must have been a change in formulation or quality 2. Does anyone know of a consulting service that will come in and work on this problem? I have made all the changes and QC checks that I can think of. The problem is obviously outside of my ability to solve. Thank you in advance for any advice or consulting recommendations! Have a lovely Wednesday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero Street, RM 230 San Francisco, CA 94115 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ROS1
Good morning histoland! Has anyone had success with the ROS1 antibody from CellSignaling? I just can't seem to get it working beyond what our pathologist call "a faint blush". I would greatly appreciate if you would be wiling to share your protocol! Have a lovely Monday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Workload tracking
Hello all, Would anyone be willing to share how they track workload? Spreadsheet? Database? Our volume has recently increased and I am looking for a way to track individual output so that I can distribute work and rotate tasks fairly. I don't want to have my "fast" people going out with repetitive motion injuries because they ended up with an unfair portion of the workload. Thank you in advance for any suggestions! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HHV-8
Good morning histonetters! I'm looking for a new source for HHV-8 (KSV), clone 13B10, antibody. CellMarque's is no longer IVD, nor is Ventana. Does anyone have a suggestion? Thank you! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leishmaniasis ctrl
Hi all, Does anyone know of a source for a Leishmaniasis control block or control slides for IHC? Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vacuolated and torn?
Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Santa Cruz antibodies
Hi all! Santa Cruz Biotech has to discontinue all goat and rabbit antibody production: http://blogs.sciencemag.org/pipeline/archives/2016/05/23/trouble-at-santa-cruz-biotechnology I hope you are all having a great Tuesday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat sectioning of FF lymph node
Good morning! My pathologists would like us to cut formalin fixed (not yet processed) tonsil tissue on a cryostat for DIF staining. Has anyone done this? I did a quick search that seemed to indicate that it was possible but that the architecture of the cells would be altered because of ice crystals and that it would be difficult to get the sections to stick. If you have any advice I would greatly appreciate it! Thanks, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
