[Histonet] Ventana's OptiView Detection
Hello All, Is anyone using Ventana's OptiView detection kit? If so please let me know what you impression is. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 (W) 720-253-7204 (C) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Evening Histology Analyst Position
Hello Fellow Histotech, InCyte Pathology is looking to fill a Histotech position on the evening shift. InCyte is a private anatomic pathology laboratory located in Spokane Valley, WA. We have 24 pathologists stationed at multiple hospital locations throughout the Inland Northwest. The specimen volume is medium-high, with about 78,000 blocks processed annually. The Histology department operates 24 hours a day Monday-Saturday. The shift that we need to fill is an evening shift. If you are interested please contact either Heather Major or Emily Clyde in our Human Resource department. They can be reached at hma...@incytepathology.com, ecl...@incytepathology.com or by calling 509-892-2700 and asking to speak with either one of them. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Evening Histotech Position Opening
Hello All, I have a full time evening position available for a registered HT or HTL in Spokane Valley, WA. The hours are 5:00 p.m. to 1:30 a.m. M-F. InCyte Pathology is a great company; it was voted one of the top 100 Washington Best Companies to work for List in 2011. Check out the website at http://incytepathology.com/. We offer an excellent benefits package including medical, dental, vision, annual bonus, 401K and profit sharing. Let me know if you are interested. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 (W) 720-253-7204 (C) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: question on H pylori
We perform an IHC Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 (W) 720-253-7204 (C) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Tuesday, June 07, 2011 1:10 PM To: lset...@childrensmemorial.org; histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] RE: question on H pylori IHC on all gastric biopsies here. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 2:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-requ...@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori..is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flow Cytometry
Hello All, We are looking into starting performing our own Flow Cytometry. Is there anyone out there that is doing this and can someone recommend a company to contact about equipment and pricing? Your help is greatly appreciated. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 (W) 720-253-7204 (C) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] saliva for glycogen hydrolysis
Room Temperature for 10-15 minutes and wash well with DI H2O after. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, February 11, 2011 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saliva for glycogen hydrolysis Those who use human saliva rather than diastase - for how long do you apply the saliva? At what temperature? Do you cover the saliva and section with a coverslip during treatment? I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC protocols
Hello All, Is there anyone who is using Ventana's BenchMark Ultra and/or XT and is successfully running DBA.44, TIA-1, GCET-1, and/or FOXP-1? If so would you be willing to share your staining protocols with me on any of these antibodies. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC antibody protocols
Hello All, I need help with information on a few Ventana BenchMark Ultra or XT protocols. We are having trouble getting HCL (DBA.44) to work. We are also in the process of working up FOXP-1, TAI-1, and GCET-1. Your help is appreciated. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing effect on DNA/RNA
Hello All, I have seen some posts on the possible damaging effects on DNA and RNA by microwave processing. Does anyone have a reference article that they can share with me? We are in the process of budgeting for next year and we are looking at microwave processors; but I want to verify if this claim is or is not valid. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processors
Hello All, I am in the process of developing the Histology budget for next three years. There have been some positive posts about a few processors recently; but if there are more opinions out there (and I know that there are) please let me know. At NSH I had the opportunity to view and hear about the most of the tissue processors on the market. There are several features that I need to consider price, reliability, ease of use/maintenance, timely and quality service, and processing TAT, just to name a few. Fellow Histology professionals you can email/contact me directly and your input is greatly appreciated. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] artifacts of sectioning
What I have used in the past is a short warm water soak, 10-15 seconds, in the water bath, after facing in and between sections, then placing the block back on ice. I would use a small 200 mL beaker filled with DI water sitting inside the water, it helps reduce junk floating the bath. Be careful to avoid over soaking. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, July 23, 2010 9:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] artifacts of sectioning It sounds like what you are describing is what we call chatter. it seems to occur most frequently with GI biopsies, esp colon polyps and usually in the abnormal epithelium. My techs tell me that cooling the block better on the ice block makes it go away. i have no personal experience, other than to say that when it occurs, it makes the slide of very poor quality. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Validation on microwaved tissue
Hello All, We have new microwave tissue processor, until this purchase all tissue has been placed on a standard VIP processor. My medical director would like for most of our 125 antibodies worked up on microwave processed tissue. I have been challenged with getting specimens to process on the instrument for IHC antibody validation purposes. Initially we will be processing GI biopsy specimens, but we will eventually progress to other biopsy tissues and so on. I have had success getting some colon cancer cases, some tonsil and other tissue. We will be mainly performing H. pylori, which is hard to get a large enough specimen to obtain a sample for this purpose. Anyway I was wondering how other labs are handling validation in this situation. Thank you for your time in this matter. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Story
Hello All, I had never heard the before starting as a Histotech trainee in Tulsa, OK. The funny story I wanted to share was similar to some others submitted. My oldest son, at five, was asked by his teacher, What does your father do? He replied, He cuts up dead people. Much to my dismay at the next parent teacher conference I was asked by the teacher what I did for a living and my reply was I am a Histotechnician and a Diener. She then asked, as most people do, What is that?. I began to explain the histology part and that I did autopsies. Then she explained what my son had told her earlier. Knowing that I had not ever told my children that I performed autopsies it came as quite a shock. So I asked my wife if she had even mentioned this to the kids and the answer was of course no. It turns out the babysitter had told them. Needless to say she no longer watches our children. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Effects of Inking on Special stains or IHC
Hello All, One of my pathologists asked the following question. Does inking tissue effect special staining or IHC results? I replied that I had never heard of this and not to my knowledge. I thought that if the chromagen was the same color of the dye then there could be some difficulty in deciphering between the two. Therefore, I am wondering if anyone has experienced a problem. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
