[Histonet] sections falling off
Hi Histonetters ! Can anyone suggest how to prevent adult mouse skin sections from falling off during in situ hybridization? How to make sections adhere better to slides? I am using poly-lysine coated slides for 5-10 micron thick skin sections. The tissue is fixed in 4% PFA and embedded in paraffin. Please help ! thanks megha -- Megha Kumar, Ph.D. Post-doctoral Research Scholar Sen-Bandyopadhyay Lab Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Kanpur, 208016 India phone - 9695830033 * * * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sections falling off in day 2 ISH
Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone suggest me why this is happening? How to prevent the slides from falling off on day 2? Thank you for all the help! regards Megha * * * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] help ! paraffin section
Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
