[Histonet] Formaldehyde concentration assay
Dear Histonet members, I would like to evaluate our NBF and formaldehyde aliquots to ensure that it is at 4%, 2% etc. I would guess I need an assay between 0 - 5%? Does anyone know a quick chemical experiment to test this? I have access to all common lab chemicals. I can see that there are kits available e.g. http://www.hannacan.com/PDF/manHI3838.pdf but I really don't need so many tests; neither do I really need the ultrasensitive formaldehyde assays for urine. Your advice is appreciated - thanks! Regards, Min-Han ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question of combining immunofluorescence and more conventional cytologic stains
Dear all, Apologies for the trouble. I am working on fresh human cancer cells on slides here in Singapore. I am trying to visualize the slides concurrently using both immunofluorescence and a more conventional nuclear stain used in pathology laboratories. I have tried Diffquik and Giemsa, but both seem to quench the fluorescence signal. I have tried a GFP-expressing cell line, and the same quenching seems to occur as well. I was wondering if anyone has ever needed to do similar work, and if so, what sort of solutions (bad pun) were adopted? Thank you! Min-Han ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
