[Histonet] Re: VCP-2001 cassette printer ink (UNCLASSIFIED)
UNCLASSIFIED Wayne, We have the VCP-3001 cassette printer and we buy the printer ribbon from Leica (800-225-3035). The stock number is V0590001F and it cost us $83.35 per ribbon which we bought several years ago. I don't know if it is the same one for your VCP-2001 but I'm sure when you call them, they can help you. Garret Miyamoto Tripler Army Medical Center Anatomic Pathology Dept., Histology Section On 04/19/13, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Fwd: [Histonet] AutoTech Embedder( Sakura) (Kiranjit Grewal) 2. New Lab In KY (Hale, Meredith) 3. email (McKenzie, Emily) 4. Calcium Stains? (McKenzie, Emily) 5. RE: Calcium Stains? (Roberta Horner) 6. Biosafety in the Mohs Laboratory (Zimmerman, Billie) 7. looking for control block of Burkitt Lymphoma (Hammel, Vicky) 8. IHC question (Shirley A. Powell) 9. VCP-2001 cassette printer question about ink (Wayne D Gill) 10. atlanta employment (Michael Bourgeois) 11. RE: RE: Pituitary gland (Tony Henwood (SCHN)) 12. RE: RE: Pituitary gland (Tony Henwood (SCHN)) 13. Re: Isopentane storage-the polling is open (Maria Mejia) 14. RE: Calcium Stains? (Tony Henwood (SCHN)) 15. 2013 CAP Inspection Success National Lab (Ian R Bernard) 16. FNA preparation (Hannen, Valerie) 17. IHC Technician job description (Vincent Rivera) -- Message: 1 Date: Thu, 18 Apr 2013 10:13:23 -0700 From: Kiranjit Grewal kira...@sbcglobal.net Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d54d92eb-04d4-490e-b059-7504ec477...@sbcglobal.net Content-Type: text/plain; charset=us-ascii Hello, I really need some feedback on this before we invest in this technology. Thanks. Kiran Sent from my iPhone Begin forwarded message: From: Kiranjit Grewal kira...@sbcglobal.net Date: April 17, 2013, 2:25:36 PM PDT To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AutoTech Embedder( Sakura) Hello Dear Colleagues, We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. Anyone currently using it and would like to share their experience is great. Thank you, -Kiran ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Thu, 18 Apr 2013 17:18:02 + From: Hale, Meredith mh...@miracals.com Subject: [Histonet] New Lab In KY To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 0E828EC51C7CC445A51E53F81B64E8C72242CB@s-irv-exchmb.PathologyPartners.intranet Content-Type: text/plain; charset=us-ascii Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com(javascript:main.compose() Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280
[Histonet] Re: Histonet Digest, Vol 100, Issue 9
Re: SOP for release of tissues and consent form Rhonda, We do not have an SOP but if you have a fax, I can send you a form that we use. Garret Miyamoto Tripler Army Medical Center - Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Wednesday, March 7, 2012 8:04 am Subject: Histonet Digest, Vol 100, Issue 9 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: xylene substitute for processing (Marcum, Pamela A) 2. unsubscribe (Sparks, Joanne) 3. Re: RE: xylene substitute for processing (Grantham, Andrea L - (algranth)) 4. Re: RE: xylene substitute for processing (Jennifer MacDonald) 5. modified Davidson's fixative on testes (Louro, Pedro) 6. Histotech Job Opening in TN-Permanent/Direct Hire (Melissa Phelan) 7. SOP for release of tissues consent form (Rhonda Ford) 8. mesocolon lymphnode treatment with aceton (Gudrun Lang) --- --- Message: 1 Date: Wed, 7 Mar 2012 15:58:13 + From: Marcum, Pamela A Subject: [Histonet] RE: xylene substitute for processing To: 'Masood, Sameena Kathryn' , Cross, Kelly , Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Message-ID: Content-Type: text/plain; charset=us-ascii Some people have issues with the smell and the other option is Anatech ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have very low odor and are also about the same price. We can't use the orange smell in our lab as some people have allergies and others just don't like it. Both work types work very well and it is a lab choice for the options, not a quality choice. Be aware most processor companies still require xylene in the clean cycle to keep your system healthy. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masood, Sameena Kathryn Sent: Wednesday, March 07, 2012 9:36 AM To: Cross, Kelly; Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: xylene substitute for processing Kelly, Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher. http://www.nationaldiagnostics.com/product_info.php?products_id=175 Good luck, Kathryn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly Sent: Monday, March 05, 2012 10:24 AM To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] xylene substitute for processing Greetings Histonet! Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? Thank you in advance for your help, Kelly Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Veterinary Pathobiology Texas Veterinary Medical Center Texas AM University College Station, TX 77843-4467 979-862-3658 Office 979-845-5149 Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. -- Message: 2 Date: Wed, 7 Mar 2012 11:09:05 -0500 From: Sparks, Joanne Subject: [Histonet] unsubscribe To: Message-ID: Content-Type: text/plain;
[Histonet] Re: Solvent Recycler
Timothy, We have the B/R 9700 ProCycler to recycle alcohol, xylene, and formalin. The Advantage must be a newer model. About 70 gal of alcohol, 40 gal of xylene, and 20 gal of formalin is recycled every 3 months. This recycler is easy to use and works well for us. As with any equipment, you need to maintain it by doing pm checks (replacing parts) as needed. Garret - Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Saturday, September 11, 2010 7:09 am Subject: Histonet Digest, Vol 82, Issue 13 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: HE+ Alcian Blue (kim.dona...@bhcpns.org) 2. solvent recycling (Timothy Chilton) 3. Liver Processing Question (Herrick, James L. (Jim)) 4. RE: solvent recycling (WILLIAM DESALVO) 5. RE: RE: Ventana vs. Leica (Makhijani, Nalini S) 6. RE: LIS, HIS question (Sharon Scalise) 7. Microm HM330 (Yu, Jian) 8. RE: LIS, HIS question (Feher, Stephen) 9. RE: solvent recycling (Feher, Stephen) --- --- Message: 1 Date: Fri, 10 Sep 2010 12:46:11 -0500 From: kim.dona...@bhcpns.org Subject: Re: [Histonet] HE+ Alcian Blue To: Deborah Faichney Cc: histonet@lists.utsouthwestern.edu , histonet-boun...@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII It wont remain turquoise as long as you use the eosin. At least this is my experience. I have known some Pathologist to read the mucin that way (purple). Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Deborah Faichney Sent by: histonet-boun...@lists.utsouthwestern.edu 09/09/2010 03:44 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] HE+ Alcian Blue Hello all, I have a request to carry out a combined staining with HE + Alcian Blue pH2.5.I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. -- Message: 2 Date: Fri, 10 Sep 2010 13:13:13 -0500 From: Timothy Chilton Subject: [Histonet] solvent recycling To: Message-ID: Content-Type: text/plain; charset=US-ASCII I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the
[Histonet] Re: Hawaii
Sarah, We had a position open at Tripler Army Medical Center but unfortunately it closed yesterday. I would suggest you try Queens Hospital, Clinical Labs of Hawaii, Kuakini Medical Center, and Kaiser Medical Center. They should have websites and you can probably inquire through them. Hope things will work out for you. Garret Miyamoto Tripler Army Medical Center Anatomic Pathology Lab - Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Friday, August 20, 2010 7:11 am Subject: Histonet Digest, Vol 81, Issue 26 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Taylor, Jean) 2. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Taylor, Jean) 3. Staining Question (Herrick, James L. (Jim)) 4. Re: Staining Question (Jack Ratliff) 5. [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Taylor, Jean) 6. Re: Corrective Action Form (Rene J Buesa) 7. Gram Control (Mark Tarango) 8. RE: Corrective Action Form (Blazek, Linda) 9. RE: Corrective Action Form (Laurie Colbert) 10. Re: Corrective Action Form (Akemi Allison) 11. Adenovirus controls (Joe Nocito) 12. Job posting for Akron Children's Hospital (McCoy, Marilynn) 13. RE: Corrective Action Form (Maria Katleba) 14. Fw: RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) (Kim Tournear) 15. looking for any type of used paraffin block files (Leroy Brown) 16. Re: Retracting microtomes (Michael Folsom) 17. RE: Corrective Action Form (joelle weaver) 18. job opening (Blazek, Linda) 19. cpt code for fused twin placentas (Houston, Ronald) 20. RE: cpt code for fused twin placentas (Weems, Joyce) 21. Hawaii (sgoe...@xbiotech.com) --- --- Message: 1 Date: Fri, 20 Aug 2010 03:25:41 +1000 From: Taylor, Jean Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: Sebree Linda A Cc: histonet@lists.utsouthwestern.edu, ih...@googlegroups.com Message-ID: Content-Type: text/plain; boundary=1282238741.aC6ACbF0.3413; charset=us-ascii Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 -- Message: 2 Date: Fri, 20 Aug 2010 04:01:22 +1000 From: Taylor, Jean Subject: [IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) To: Sebree Linda A Cc: histonet@lists.utsouthwestern.edu, ih...@googlegroups.com Message-ID: Content-Type: text/plain; boundary=1282240882.203a0.15929; charset=us-ascii Dear Sir/Madam, Your email was unable reach the intended person that you were sending it to. For more information on our business please click on the following link: [1]Click here for our website We look forward to your continued business in the future. Regards, Webmaster References 1. http://www.downwind.com.au/avdir/rd.php?id=7564 -- Message: 3 Date: Thu, 19 Aug 2010 14:22:33 -0500 From: Herrick, James L. (Jim) Subject: [Histonet] Staining Question To: Message-ID: Content-Type: text/plain; charset=us-ascii Good afternoon everybody, Would anyone be willing to offer their wisdom on the best etching solution for 50 to 75 micron thick jaw specimens embedded in MMA? The stain we want to use is a Trichrome. Thanks in advance for your help. Jim -- Message: 4 Date: Thu, 19 Aug 2010 14:28:10 -0500 From: Jack Ratliff Subject: Re: [Histonet] Staining Question To: Herrick, James L. (Jim) Cc: Message-ID: Content-Type: text/plain; charset=us-ascii 0.7% Formic Acid for 30 seconds with sonication, then rinse well in DI water. Jack On Aug 19, 2010, at 2:22 PM, Herrick, James L. (Jim) wrote: Good afternoon everybody, Would anyone be willing to offer their wisdom on the best etching solution for 50 to 75 micron thick jaw specimens embedded in MMA?
[Histonet] Re: Tissue processor times
Carol, We just obtained a VIP 6 processor and we have a program for routine overnight processing. It seems to work well. I will send or fax it to you by Monday. The Tissue-tek sales rep that installed and trained us, mentioned about increasing the times in the alcohols for fatty/breast tissue. You can try this and make any adjustments to the program I am sending. We have two stations of formalin at two hours each. As a side note, we have two Excelsior processors also and except for a few glitches, they seem to work fine. Take care. Garret Miyamoto Lead histotech, Anatomic Pathology Dept. Tripler Army Medical Center - Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Friday, December 4, 2009 8:04 am Subject: Histonet Digest, Vol 73, Issue 8 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Cerner Millennium Scroll Capabilities? (Evans, Andria B) 2. Histology Supervisor Job (K.C. Carpenter) 3. Formaldehyde Vapors (Walter Benton) 4. RE: what book to study for registry? (Carol Bryant) 5. RE: [SPAM-HC] - [Histonet] what book to study for registry? - Emailfound in subject (Cazares, Ruth) 6. RE: what book to study for registry? (Cheri Miller) 7. mistake (Cheri Miller) 8. one more time. This is what I meant to send (Cheri Miller) 9. tissue processor times (Carol Bryant) 10. THANK YOU (Kathy M. Gorham) 11. RE: one more time. This is what I meant to send (Cheri Miller) --- --- Message: 1 Date: Fri, 4 Dec 2009 09:54:53 -0500 From: Evans, Andria B Subject: [Histonet] Cerner Millennium Scroll Capabilities? To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 Does anyone out there have the capability to scroll with your mouse wheel in cerner millennium? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open. -John Moe Maturity is accepting imperfections. CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ -- Message: 2 Date: 4 Dec 2009 09:55:48 -0500 From: K.C. Carpenter Subject: [Histonet] Histology Supervisor Job To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=utf-8 Dear Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. One position in particular I want to tell you about is a 1st shift Histology Supervisor job with one of the top ranked teaching hospitals in the country. Located in New York City, this hospital is an award winning facility that's consistently ranked in the top 10% in the annual Best Hospital issue of US News World Report. Requirements
[Histonet] Re: Shandon Excelsior Tissue Processor
Tasha, We have the Excelsior which we received a few weeks ago. We have not used it yet for patient specimens but will very soon. Our test runs went well except some breast tissue (probably cut too thick). Overall, the pathologist was happy with the results. There are many good features, but one of them was the potential savings of alcohol solutions. It has a built in hydrometer that measures the percentage of alcohol in the containers and it will let you know when to change it. We are also looking into the Tissue Tek VIP. We have the 300 model which we like but it is nearing it's life expectancy. Garret Miyamoto Tripler Army Medical Center - Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Saturday, August 15, 2009 7:03 am Subject: Histonet Digest, Vol 69, Issue 15 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. streptavidin peroxidase (MKing) 2. HT opening in Colorado (Matthew Lunetta) 3. unsubscribe (Woodward, Denise) 4. Job Opening (Konni Black) 5. Shandon Excelsior Tissue Processor (Tasha Fraser) 6. Can DAB block an epitope?? (Amos Brooks) --- --- Message: 1 Date: Fri, 14 Aug 2009 13:16:40 -0400 From: MKing Subject: [Histonet] streptavidin peroxidase To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Sigma E-2886 extravidin peroxidase, 1:1000 in 0.1 M PBS or sodium acetate on almost every IHC variation (fixation, species, primary) we do. Supposed to be less nonspecific binding than streptavidin. Mike King UF Pharmacology Therapeutics -- Message: 27 Date: Fri, 14 Aug 2009 09:56:56 -0700 From: Karen Cai Subject: [Histonet] Need help on Streptavidin-HRP conjugate for IHC To: Message-ID: Content-Type: text/plain; charset=us-ascii Hi, I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it is a good choice since the background is kinda high. Is there anybody having some suggestions? Which kind of steptavidin-HRP are you using for DAB-IHC? From which company? Thank you very much for your kind reply, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax http://www.prosci-inc.com www.prosci-inc.com -- Message: 2 Date: Fri, 14 Aug 2009 14:02:37 -0600 From: Matthew Lunetta Subject: [Histonet] HT opening in Colorado To: Message-ID: Content-Type: text/plain; charset=US-ASCII Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetailnextEvent=doSearchPositionscobrandId=9000masterId=luh001accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88FpositionId=511568urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) -- Message: 3 Date: Fri, 14 Aug 2009 16:20:34 -0400 From: Woodward, Denise Subject: [Histonet] unsubscribe To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii -- Message: 4 Date: Fri, 14 Aug 2009 13:27:50 -0700 From: Konni Black Subject: [Histonet] Job Opening To: Message-ID: Content-Type: text/plain; charset=Windows-1252 Hello All, There is a new histology lab opening in St. Augustine, Florida. Beautiful area on the Palm Coast. Great pay and working conditions. Extremely flexible hours. In fact, create your own schedule! Contact me for details or send resume to kbl...@digestivelth.com Don't miss this ground floor opportunity. Konni Black -- Message: 5 Date: Fri, 14 Aug 2009 14:47:54 -0700 From: Tasha Fraser Subject: [Histonet] Shandon Excelsior Tissue Processor To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Would
[Histonet] Re: Specimen Release
Laurie, I can fax you a copy of a release form if you want. Garret Miyamoto Tripler Army Medical Center - Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Saturday, August 8, 2009 7:03 am Subject: Histonet Digest, Vol 69, Issue 8 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Specimen Release (Laurie Colbert) 2. Good Fibrin stain with Movat's? (Judith L. Williams) 3. Thomas Crowell is out of the office. (thomas.crow...@novartis.com) 4. Re: Specimen Release (Richard Cartun) 5. Peggy-cement in bone (Vicki Kalscheur) 6. Stain for decalcified GMA embedded bone (Yash Kolambkar) --- --- Message: 1 Date: Fri, 7 Aug 2009 10:17:21 -0700 From: Laurie Colbert Subject: [Histonet] Specimen Release To: Message-ID: Content-Type: text/plain; charset=us-ascii Does anyone have a procedure they would be willing to share with me regarding the release of specimens to the patient? We are revising our whole process and I'm interested in what others are doing. Laurie Colbert -- Message: 2 Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) From: Judith L. Williams Subject: [Histonet] Good Fibrin stain with Movat's? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 -- Message: 3 Date: Fri, 7 Aug 2009 13:43:20 -0400 From: thomas.crow...@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 08/07/2009 and will not return until 08/10/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. -- Message: 4 Date: Fri, 07 Aug 2009 14:35:23 -0400 From: Richard Cartun Subject: Re: [Histonet] Specimen Release To: Laurie Colbert , Message-ID: Content-Type: text/plain; charset=US-ASCII We no longer release specimens to patients. If they want their tissue they have to go through a funeral home. Here in CT, funeral homes are the only agencies licenced to bury or incinerate human tissue. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Laurie Colbert 8/7/2009 1:17 PM Does anyone have a procedure they would be willing to share with me regarding the release of specimens to the patient? We are revising our whole process and I'm interested in what others are doing. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 5 Date: Fri, 7 Aug 2009 14:06:28 -0500 From: Vicki Kalscheur Subject: [Histonet] Peggy-cement in bone To: Histonet Discussion Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi Peggy, From my experience, during the decal process the cement will fall out or loosen enough so that you can gently remove it and maintain fibrous ingrowth and detail. I have removed ceramic; implants; stents and cement using a scalpel and then embedded in paraffin for cuts on a sledge microtome. The detail is beautiful even without the cement present. If you have the capability, a corresponding section in PMMA can be processed. ~ Vicki -- Message: 6 Date: Fri, 07 Aug 2009 15:12:52 -0400 From: Yash Kolambkar Subject: [Histonet] Stain for decalcified GMA embedded bone To: histonet@lists.utsouthwestern.edu
[Histonet] Re: Recycled Formalin
- Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009 7:15 pm Subject: Histonet Digest, Vol 65, Issue 17 To: histonet@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Large coverslips? (Va Paula Sicurello) 2. HI Downdraft fume extractor for sale (Cathy Mayton) 3. breaking glass jar/vials for MMA embedded specimens (Cathy Mayton) 4. AW: [Histonet] MAMMOGLOBIN (Gudrun Lang) 5. Re: AE1/AE3 nuclear staining (Rene J Buesa) 6. Re: AE1/AE3 nuclear staining (Mark Tarango) 7. RE: Tissue Capture Pen (Atoska Gentry) 8. RE: I'm outta here (Smith, Allen) 9. Controls needed! (Knutson, Deanne) 10. Fixation question - Cerebellar granular cells (Guillermo Palchik) 11. Recycled formalin (Richard Cartun) 12. RE: Unsubscribe (Tony Henwood) 13. RE: gi microwave processing (Tony Henwood) 14. RE: AE1/AE3 nuclear staining (Tony Henwood) 15. Re: Recycled formalin (Greg Dobbin) 16. Re: Fixation question - Cerebellar granular cells (TF) 17. Re: Staying... (Bernie Taupin) 18. Re: Staying... (Bernie Taupin) 19. RE: Unsubscribe (aa aa) 20. Re: Whole human feet (Bernie Taupin) 21. Re: FREEZY spray (Bernie Taupin) 22. Re: FREEZY spray (Akemi Allison-Tacha) 23. Re: FREEZY spray (Bernie Taupin) --- --- Message: 1 Date: Tue, 7 Apr 2009 10:17:43 -0700 (PDT) From: Va Paula Sicurello Subject: Re: [Histonet] Large coverslips? To: histonet@lists.utsouthwestern.edu,yvan lindekens Message-ID: Content-Type: text/plain; charset=utf-8 Hi Yvan, Does it have to be a coverslip? There are some mounting media that harden and form a barrier with optical qualities similar to that of glass. It might not be a perfect solution, but it might work. I think the stuff I used was called Crystal Mount (?). Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr.., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 4/7/09, yvan lindekens wrote: From: yvan lindekens Subject: [Histonet] Large coverslips? To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:05 AM Hi all, I���m looking for some kind of a DIY coverslip or a tape, plastic foil��� anything usable to cover some large slides (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical and zoological sections mounted in Canada Balsam. Does anyone has a (cheap���) solution for this? It doesn���t need to be pristine optical quality as the slides are primarely intended to be used on an overhead projector in the class room, but the possibility of viewing them under low power (40x - 100x) would be a real advantage. Thanks in advance four your wisdom and knowledge! Yvan. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Tue, 7 Apr 2009 10:23:02 -0700 From: Cathy Mayton Subject: [Histonet] HI Downdraft fume extractor for sale To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 I have a HI downdraft fume extractor for $50 and we will provide carbon to refill the drawer. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 -- Message: 3 Date: Tue, 7 Apr 2009 10:28:44 -0700 From: Cathy Mayton Subject: [Histonet] breaking glass jar/vials for MMA embedded specimens To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 Yes, the scintillation vials are notorious for shattering in the freezer. However, if you put them in a plastic container with a lid on, the shattered glass will be contained and not all over your freezer. When breaking larger glass containers, again place them in the freezer, remove from the freezer after 20 minutes or so, take the lid off, wrap jar in paper towels and hold the ends closed. Wear glovesand eye protection in case shards of glass escape. Strike the jar with a hammer and unroll the paper towel over a garbage can. Rinse the block in tap water to remove any small shards of glass. This is a great way to vent frustration!! Cathy
