[Histonet] Open Positions

[Nails, Felton via Histonet]

I have two open positions at Texas Childrens Hospital.
There is one in the IHC lab and one in Special Stains.
If interested apply at Texaschildrenshosptial.org


Thanks,
Felton L. Nails

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[Histonet] CPT Code Changes

[Nails, Felton]

Was just wondering how everyone is handling the new changes to IHC 88342 and 
the use of 88341?

Thanks.

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RE: [Histonet] Non-cert. histo classes

[Nails, Felton]

My opinion is that it is a good idea because many hospitals are shutting down 
their EM department because of a lack of skilled workers.
So the more skills they have the better for them.
Let me know if any of your students want to move to Houston Texas.

Felton Nails
Texas Children's Hospital 
832-824-1248

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Tuesday, September 02, 2014 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Non-cert. histo classes

Greetings

I am a regular reader of this list, but from a peripheral setting.

I work in an electron microscopy certificate program. Our students are prepared 
to do either or both certificates in EM, biological and materials.

During their biological training, students are instructed in thin sectioning, 
specimen prep., etc. I would like to get ideas about how valuable adding more 
light microscopy specimen prep, staining, and sectioning might be for these 
students. Would it be good for them to have these skills and knowledge but not 
a histotech cert.?

I thought about doing a full cert. program, but am daunted by the requirements 
to set one up and I don't want to compete with other better established 
programs near by.

Bottom line is I would like to know if teaching basic histo tech skills, w/o 
certification, is a viable path. Could students leverage these skills into jobs 
at non-health care type facilities? Could having these skill help them complete 
a certificated program if they wanted to take that direction later? 

Your input will be valuable to both me and my students.

Thanks

Jon

Jonathan Krupp
Applied Science, Business  Technology
San Joaquin Delta College
5151 Pacific Ave.
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College









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RE: [Histonet] Should I leave histology world

[Nails, Felton]

I always tell my students if you can cut, you can get a job. It appears that 
you school did not properly prepare you for the demands of an average histology 
job.
You need to take every opportunity to work on your craft and the major focus of 
histology is cutting. With 6 to 7 years of experience you are expected to know 
the basics and cutting is basic.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech
Sent: Tuesday, June 03, 2014 3:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Should I leave histology world

Hi everyone,

I wouldn't give too much detail information as the histology world is very 
small and everyone knows everyone.

I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went 
to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs 
I have changed jobs 3 times. All the jobs were graveyard shifts. The first 
place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 
places I won't mention and I currently still have a histology job. My problem 
is all the places I worked were factory style lab work and they all did derm 
work. In my career I really only embedded most of the time. I did occasional 
other stuff like special stains both by hand and using Dako Artisan and other 
things like cytology cytospin. But I never got to develop in cutting. My first 
job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me 
and put me back to embed. My 2nd job put me to cut the last 2 months (full 
8hrs) I was working there. My current job I have been cutting since April 2014 
( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was 
embedding most of the time before th cutting started). I was told by my 
director I need to speed up in cutting because corporate is asking why I am not 
increasing in speed. And if I don't speed up eventually then they will have to 
demote me to a lab aid and give me a pay cut. (where I work and the state I 
work in they have lab aids doing alot of stuff without being certified, it 
wasn't like that in the other state I am original from as you have to be state 
licensed and ascp) I sometimes laugh inside my head because before my director 
hired me I told him I don't have alot experience in cutting. 

Now everywhere I have gone...speed is the name of the game. They say they care 
about quality but in the end if you can't put up then you will be put out!  So 
I am just thinking I should just get out of histology world all together. Every 
where I have worked unfortunately have management who believe quantity over 
quality. OR Do you guys think I need more time cutting to develop speed? 
Beforehand I did need a little learning curve to cut and I have gotten through 
that now. It's just the speed that is killing me. And I also see if anyone at 
my work detours me for any reason like for example data entry person from front 
desk ask for missing gross dictation, then that lost time is very hard to 
recover as I am not s fast to recover. I feel I may have to become very 
rude(not help) with everyone I work around in order to stay glued to my seat 
when I am cutting my blocks. One thing I want to say also...until this day I 
never been written up for quality issues and I never lost any tissue while 
embedding. Embedding I am fast as most histotech (1 block a min or most times 
30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on 
same plane, tissue embedded with proper orientation and follow any other 
necessary embedding instructions. ) I just feel I haven't done my time in 
cutting as I did in embedding to become a fast cutter. I don't know if its 
because of working in a derm lab that management won't wait too long for you to 
develop like maybe a hospital lab may do. I was also thinking to find another 
histo job but not mention any of my experience so expectation won't be so high 
and I can get more time to develop. All of this also causes alot of stress and 
anxiety as it gets hard to coop with.  What do you guys think and how I should 
go about with this. I am also not limited to histology. I have expertise in 2 
other major fields that I wont mention because I don't want to be identified.  
I am also in my late 20's. Thanks for reading my post and I await your opinions 
as some of you all are veterans in the field of histology.

Thank you
Alpha Histotech (ASCP HT)


  
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[Histonet] Req Storage

[Nails, Felton]

Has anyone started scanning requisitions and discarding the original AP reqs?
If so does this meet the CAP retention requirement?





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RE: [Histonet] Blocks cracking

[Nails, Felton]

Are the blocks that are not being sent cracking? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 06, 2014 8:08 AM
To: Erica Canal; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Blocks cracking

Contact Leica Microsystems because they are very good in troubleshooting their 
products and for sure will know.
It is better than start guessing after the fact!
René J.



From: Erica Canal eca...@usc.edu
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 5, 2014 6:51 PM
Subject: [Histonet] Blocks cracking


Hello all, 
We are wondering harder paraffin makes blocks more fragile.
We have been using Leica's Formula R and on a few occasions  when we have 
mailed blocks out, we receive them back in pieces. Could this be because of 
type of wax we are using? The quality of our slides is great, we just seem to 
be having a problem lately with our blocks breaking after they have been sent 
out  we want to try to prevent that.

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[Histonet] neuropath stains

[Nails, Felton]

Are the basic muscle and nerve biopsy stains being performed manually or has 
someone automated these stains?




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RE: [Histonet] Leica slide printers

[Nails, Felton]

If you are referring to the IPS slide writer, I would say continue looking it 
is very unreliable. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine 
Simonson
Sent: Monday, October 21, 2013 1:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica slide printers

I was wondering if anybody out there in Histoland had any experience with the 
new Leica slide printer?  What are your thoughts?

Thanks in advance,

Catherine
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[Histonet] Body bags usage.

[Nails, Felton]

When you have a patient that expires,  do you require that the bodies be 
brought down in body bags for an autopsy or funeral home pick up?




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[Histonet] BK Virus

[Nails, Felton]

Any ideas in histoland where I can find control tissue for BK Virus IHC?




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[Histonet] test

[Nails, Felton]

9-26-2013




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RE: [Histonet] RE: Submitting Questions

[Nails, Felton]

I have even tried copying the following link 
(histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make 
a post. 

-Original Message-
From: nancy lowen [mailto:clayca...@yahoo.com] 
Sent: Wednesday, September 25, 2013 10:49 AM
To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha 
Ward-Pathology
Subject: Re: [Histonet] RE: Submitting Questions

Me too!

On Tue, 9/24/13, Martha Ward-Pathology mw...@wakehealth.edu wrote:

 Subject: [Histonet] RE: Submitting Questions
 To: Nails, Felton flna...@texaschildrens.org, 'Joanne Clark' 
jcl...@pcnm.com, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
 Date: Tuesday, September 24, 2013, 12:54 PM
 
 I have had the same problem over the
 past few months...
  
 Martha Ward, MT (ASCP) QIHC
 Manager
 
 Molecular Diagnostics Lab
 Medical Center Boulevard  \  Winston-Salem, NC 27157  p 336.716.2109  \  f 
336.716.5890  mw...@wakehealth.edu
  
  
  
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu]
 On Behalf Of Nails, Felton
 Sent: Tuesday, September 24, 2013 2:58 PM
 To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu
 Subject: [Histonet] RE: Submitting Questions
 
 I am in the same situation??? 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu]
 On Behalf Of Joanne Clark
 Sent: Tuesday, September 24, 2013 12:54 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Submitting Questions
 
 Can anyone out in histoland explain to me why my submitted  questions are not 
making it through to histonet?  I  submitted a question on the 19th of 
September, and I still  have not seen it appear in the group emails I get  
daily.  I sent the question to the correct address (I  checked it to be sure).  
When I respond to someones  question, I can see my responses, I just can't seem 
to get  an original question submitted.  Can anyone tell me  what I am doing 
wrong?
 
 
 
 
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and do not disclose its contents or take any action in  reliance on the 
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[Histonet] test

[Nails, Felton]

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[Histonet] 1

[Nails, Felton]

test



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[Histonet] RE: Submitting Questions

[Nails, Felton]

I am in the same situation??? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Tuesday, September 24, 2013 12:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Submitting Questions

Can anyone out in histoland explain to me why my submitted questions are not 
making it through to histonet?  I submitted a question on the 19th of 
September, and I still have not seen it appear in the group emails I get daily. 
 I sent the question to the correct address (I checked it to be sure).  When I 
respond to someones question, I can see my responses, I just can't seem to get 
an original question submitted.  Can anyone tell me what I am doing wrong?




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are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.

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[Histonet] RE: Recycled Xylene

[Nails, Felton]

We have been using recycled xylene for years and have found that it only 
present a problem on the clean cycle of the processor.
Everywhere we have not experienced a problem, so we use new xylene on the 
cleaning cycle. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Monday, July 29, 2013 10:47 AM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Recycled Xylene

Does anyone out there have any problems with using recycled xylene to  clean 
the tissue processor?   I was just told that we should not use recycled xylene 
to clean the tissue processor.  How about using recycled xylene for either 
processing or staining?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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[Histonet] RE: Sections

[Nails, Felton]

I have heard of both methods, currently we put double ribbons on one slide.
The only time we have repeats is if the initial cut is superficial. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, July 23, 2013 2:22 PM
To: 'Martin, Gary'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Sections

If placing more than 1 section of the same level on a slide, I've always cut 
three slides. I've never had a pathologist who only wanted to see one level.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary
Sent: Tuesday, July 23, 2013 2:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sections

One of our pathologist has requested a change in the way we cut GI specimens.  
We presently put three levels on one slide. Exp.  we face the block, cut a 
ribbon and take one section of that ribbon and catch on the slide. We then 
progress into the block and repeat the process until we have three levels on 
the slide.  The new suggestion is to simply face the block, create a ribbon, 
and take three to four section of that ribbon and that's it no more progression 
into the block.  The thinking is that the Pathologist will see what they need 
to in those levels, and if not they will call for deepers. I have never cut 
like this and just wanted to hear some thoughts on this method.

Thanks

Gary

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[Histonet] RE: Special Stainers

[Nails, Felton]

I would like to be included on this request also, We currently have the DAKO 
special stainer and are looking to see what else is available.
The DAKO unit is very good but we are being forced to sign a reagent agreement 
with a minimum commitment even though we own the unit.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Kent
Sent: Friday, May 17, 2013 12:46 PM
To: histonet
Subject: [Histonet] Special Stainers

Hello All,

Can you share your experience or recommend an automated Special Stainer for 
clinical path? We are considering adding an additional.

Thanks,

Mike 






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[Histonet] RE: Pathology requisitions

[Nails, Felton]

What system are you using to place these orders in the OR? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, March 26, 2013 2:27 PM
To: 'Duddey, Aimee'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Pathology requisitions

We have an electronic requisition that looks like the paper that we use in some 
units. The ORs didn't use it because they didn't have good access to computers 
from each room. But the GI labs and Breast Health used it successfully.

A Pathology is ordered and fields pop up that the info is typed into. Two 
copies of the req prints - one in Histolgy with a bar code and one in the unit 
that is sent down with the specimen. That has served us well for several years.

Unfortunately, with our partnerships with Emory and the upgrade to the system, 
we have to go back to paper. Maybe, someday..



Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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review, use, disclosure, or distribution is prohibited. If you are not the 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee
Sent: Tuesday, March 26, 2013 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology requisitions

Our facility, as many do now, uses electronic physician order entry.  We 
struggle with the pathology and cytology portion of this.  We still require the 
manual requisition to be able to obtain the necessary information like clinical 
history, specific specimen info, etc.  Does anyone have a good electronic 
solution for the pathology/cytology requisition?  Any ideas would be greatly 
appreciated.



Aimee Duddey

FirstHealth Moore Regional Hospital

Pinehurst, NC 28394

adud...@firsthealth.org

910-715-5286

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RE: [Histonet] Devasting news on 88305TC component

[Nails, Felton]

Before you holler political and blame the candidates, ask yourself who was hurt 
most by POL's?
Large reference labs.
With this change they will get back the business because it will not be 
profitable to establish a POL.
Also they lobbied for and increase on the PC, reference have pathologist, most 
POL's don't.
Just my thought 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Friday, November 02, 2012 11:52 AM
To: 'Cristi Rigazio'; Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: RE: [Histonet] Devasting news on 88305TC component

AMEN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, November 02, 2012 9:32 AM
To: Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: Re: [Histonet] Devasting news on 88305TC component

Political yet?!  Seriously!  52%, while the PC is increased 2%... But in case 
anyone wondered both candidates for President are looking for the middle class! 
 Unbelievable!

Sent from my iPhone

On Nov 2, 2012, at 8:28 AM, Brendal Finlay 
brendal.fin...@medicalcenterclinic.com wrote:

 http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverrid
 e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtl
 t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201
 3_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr
 
 
 Brendal Finlay, HT (ASCP)
 Medical Center Clinic
 brendal.fin...@medicalcenterclinic.com
 850.474.8758
 http://medicalcenterclinic.com
 -Original message-
 From: Davide Costanzo pathloc...@gmail.com
 Date: Fri, 02 Nov 2012 10:09:18 -0500
 To: Webster, Thomas S. twebs...@crh.org
 Subject: Re: [Histonet] Devasting news on 88305TCcomponent
 
 That is devastating! Do you have a link to this information?
 
 Sent from my iPhone
 
 On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. wrote:
 
 Devastating I meant
 
 
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RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) andfuture trends

[Nails, Felton]

Let's face it POL's were established to make more money for the groups, which 
takes money from the big reference labs who have large lobbyist groups.
HUM, I wonder who maybe driving these efforts. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Wednesday, October 31, 2012 10:07 AM
To: Rene J Buesa; Kaye Ryan
Cc: Histonet
Subject: RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

Here we go with the political opinions again. They have no place on this forum. 
It's bad enough that Facebook is polluted with them.

Have a nice day!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 31, 2012 11:03 AM
To: Kaye Ryan
Cc: Histonet
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

Kaye:
Sure!
The odd thing that struck me is that this fictitious scenario was planted 
on Histonet just a few day before the presidential election and I am suspicious 
of the final intent of the posting.
Is it to link a frightening scenario with the so called Obama care?
Is it the possible solution of this imaginary scenario to eliminate Obama 
care as has been promised by Romney?
Is this a way to get a few additinal votes for Romney? I would not be surprised 
at all!
I am always very suspicious of coincidences because coincidences do not 
exist.
René J.



From: Kaye Ryan kr...@nfderm.com
To: Rene J Buesa rjbu...@yahoo.com
Sent: Wednesday, October 31, 2012 10:55 AM
Subject: RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

THANK YOU SO MUCH, RENEE!

Kaye

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 31, 2012 10:51 AM
To: Brendal Finlay; Webster, Thomas S.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

To everybody worrying for something that has NOT happen yet and that NOBODY 
knows if will happen.
These are pure speculations.
Do not put the cart before the horses. I do not think that this scary scenario 
is in the near future.
René J.



From: Brendal Finlay brendal.fin...@medicalcenterclinic.com
To: Webster, Thomas S. twebs...@crh.org
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wednesday, October 31, 2012 9:39 AM
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) and 
future trends

This is disturbing news. As an employee of an in-house lab (which started in 
1996/1997) that does mostly skins, GI biopsies, and outpatient surgery 
specimens I'm pretty disheartened to hear about the 88305 issue. Melanoma 
excisions, prostates (even lower block # cases, we don't always get 12), breast 
biopsies, and other more difficult cases can be a lot of work on both the 
professional  technical end of things.  

As for prostate biopsies, CMS has already lowered reimbursement with the G 
codes. This is despite the wording that they are for saturation biopsies. We 
rarely have saturation biopsies, but Medicare denies us the 88305 charge if 
more than 5 specimens. 

Other insurance companies tend to follow their lead  after a little time.  I 
believe reimbursement is 50-75% less for 5-20 biopsies, but don't quote me on 
that.  I expect we may see the end of saturation and multi-container prostate 
biopsies in the near future. 

Another issue for many outpatient labs in my area is that larger insurances are 
requiring their patients to go to large multinational labs.  We cannot accept 
many PPOs or Medicare replacement plans because of this. 

I feel it can be a disservice to the patient because they do not get the same 
personal, local service with good turn around times. Even my insurance requires 
me to go to one of these labs where I feel inconvenienced and frustrated at the 
wait time required to submit my sample and get results to my physician. 

On Oct 31, 2012, at 8:17 AM, Webster, Thomas S. twebs...@crh.org wrote:

 Here is what CAP has on their website about the issue.
 Only the TC of 88305 is being discussed for 2013. We should know fairly soon 
 the decision.
 More codes have been flagged as overvalued as well that could be cut for 2014 
 (PC and TC at this point).
 
 http://www.cap.org/apps/docs/advocacy/advocacy_issues/revaluation.pdf
 
 
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RE: [Histonet] Number of blocks

[Nails, Felton]

Can you list the article, because I to would be interested. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, October 25, 2012 10:00 AM
To: Will Chappell; Hannen, Valerie
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Number of blocks 

Will: 
I would like to do that very much but unfortunately Histonet is set in a way 
that they do not accept atttachments and any e-mail with them will not go 
through.
René J.



From: Will Chappell cha...@yahoo.com
To: Hannen, Valerie valerie.han...@parrishmed.com
Cc: Rene J Buesa rjbu...@yahoo.com; Dorothy Ragland-Glass 
techman...@yahoo.com; Histonet@lists.utsouthwestern.edu 
Histonet@lists.utsouthwestern.edu
Sent: Thursday, October 25, 2012 10:37 AM
Subject: Re: [Histonet] Number of blocks 

In fact, can you share them with all of histonet?

Sent from my iPhone

On Oct 25, 2012, at 7:33 AM, Hannen, Valerie valerie.han...@parrishmed.com 
wrote:

 Rene,
 
 I have been asked in the past about productivity in our department. Can you 
 share those articles with me as well?
 
 Thanks!!
 
 Valerie
 
 Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief 
 Parrish Medical Center
 951 N. Washington Ave.
 Titusville, Florida 32976
 Phone:(321) 268-6333 ext. 7506
 Fax: (321) 268-6149
 valerie.han...@parrishmed.com
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J 
 Buesa
 Sent: Thursday, October 25, 2012 10:18 AM
 To: Dorothy Ragland-Glass; Histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Number of blocks
 
 Hi Dorothy:
 Your manager is wrong and probably influenced by some productivity 
 consultant trying to appear tough or preparing to justiffy a staff 
 reduction.
 The average sectioning productivity obtained in 325 histology laboratories 
 (221 in the US and 114 in 24 foreign countries) is 24 blocks per hour.
 Under separate cover I am sending two articles dealing with this issue and 
 that of staffing that you will be able to show to your manager.
 René J.
 
 
 
 From: Dorothy Ragland-Glass techman...@yahoo.com
 To: Histonet@lists.utsouthwestern.edu
 Sent: Wednesday, October 24, 2012 8:38 AM
 Subject: [Histonet] Number of blocks
 
 It was annouced by a histo lab manager that techs are expected to cut 40-50 
 blocks per hour. That seems to me to be rather high. I don't see quality 
 slides being turned out. It is quantity and profit above patient care. I am 
 old school, and I remember something about quality and patient first. 
 Besides  what kind of impact on morality of the techs, back problems and 
 carpal tunnel syndrom is laying ahead for the cutter after cranking the 
 microtome repeatedly that many blocks without a break.
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RE: [Histonet] Reliable Histology Team Member to embed and cut for you.

[Nails, Felton]

What was the initial purpose for starting the Histonet, I assume it was to 
offer a forum for Histotech to get assistance with problems they may encounter.
It offends me when there are those that feel they can control what is posted, 
the histonet is for the group not a few individuals. 
Histocare may be excessive in their postings but if it does not apply to you 
don't read it.
They are no different then the other staffing agencies posting job listing 
because in turn they are promoting their company.
Both provide a service, again if you don't need their service don't read it. 
However someone may need their services and they should not be excluded.

Just my two cents worth! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori Harris
Sent: Wednesday, October 03, 2012 8:12 AM
To: Contact HistoCare; Jay Lundgren
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Reliable Histology Team Member to embed and cut for you.

Well, if I had ever thought of using your services in the past, after reading 
this post I would never consider it. You should have quit responding a couple 
of posts ago.

Lori

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact 
HistoCare
Sent: Tuesday, October 02, 2012 7:49 PM
To: Jay Lundgren
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reliable Histology Team Member to embed and cut for you.

Ok, let me put this issue to bed.
It's fine you feel you need to be the
voice for those who have all the help they need and will never need the 
services of a histology professional to help cover staffing shortages or 
inadequate/ineffective staffing.

There are thousands of subscribers to this list and I respect every single one 
of them. HistoCare is for those who NEED professional assistance on an interim 
basis. And let's be clear, I'm not just a tech looking for job once in a 
while. I'm a professional extending my expertise and invaluable abilities to 
help when the need arises on relatively short notice without compromising 
patient care.

Your messages seem to have a very petty tone and not respectful of the nature 
of the work we do. It's almost as if you are not even of this profession.
I care about all those people who go to doctor to have a million tests done to 
see what's wrong with them and have to wait days and weeks before they get any 
kind of news to settle their nerves or some glimmer of hope. I want to minimize 
that inconvenience as much as I can.

I am a real person first, a histology professional second on the other side of 
this email just like there is a real patient on the other side of that slide.

You only care about HistoCare advertising on one of the few forums histology 
professionals have to exchange thoughts, ideas, and RESOURCES. If the best you 
can add to histonet is criticism, it doesn't need you or those like you.

This is a small community with very high turnover, low job satisfaction, some 
employed individuals with marginal or inadequate abilities, and little interest 
in others to want to break into this profession. Heck even the ones that's been 
his field for a long time express dismay, let alone the newbies who can't get 
the proper support from their own supervisors! Good grief!

Those who rely on histonet for advice and ideas should also be able to search 
for dependable lab support and hope that there is a good resource for them to 
call upon.

Please do me a favor and respectfully bow out and resist the urge to respond 
further as I have no interest in debating. I'd rather spend my time being 
productive.

There isn't a policy specifically stating i can't make HistoCare available for 
those who may search for assistance. Lets not forget Histonet is a courtesy to 
us all for interests in histology and what it is not, is a vehicle to complain.

I was initially open to your suggestions for alternatives and offered you the 
opportunity to respond with solutions acceptable to 'you' but I see you would 
rather be a complainer than a helper.

Any future responses from you referring to this matter directly or indirectly 
will be considered harassment and forwarded to the appropriate parties.

Sincerely HistoCare

www.HistoCare.com



On Oct 2, 2012, at 4:19 PM, Jay Lundgren jaylundg...@gmail.com wrote:

 I am not the only forum member who is concerned about this.  I have received 
 private messages from others who agree with me and choose not to reply to 
 all.  I will not repost them out of respect for their privacy.

 The facts that you take what you do seriously, or are not disrespectful are 
 moot.

 The fact that does apply here is that advertising is not allowed on Histonet.

 The staffing agencies that occasionally post on here are offering a 
 list of open jobs.  You are soliciting for your services, three times 
 in one week. (9/25, 

[Histonet] RE: Fragmented Biopsy Samples

[Nails, Felton]

Try using histoscreen cassettes and that will eliminate the need for wrapping 
the tissue.
If still insist on wrapping, ensure that they are dipping the tissue paper in 
formalin prior to putting the tissue on the paper.
Also at embedding, if the tissue is stuck to the paper, take a blade and put it 
against the tissue and gently push to loosen the tissue, repeat this the length 
of the tissue. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Thursday, August 30, 2012 10:41 AM
To: Willis, Donna G.; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Fragmented Biopsy Samples

Donna, try the new SecureSette from ThermoFisher. They enclose the sample at 
grossing and do not require manipulation at embedding - just add wax and cool. 
Very interesting product. We tried them and they work well for some samples. 
However, they only accommodate straight needle bx of 2cm or less. Our prostate 
cores were 2.5 cm at times so it didn't quite fit.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94143

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.mor...@ucsfmedctr.org




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Thursday, August 30, 2012 8:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fragmented Biopsy Samples

Any hints out in Histoland on how to eliminate thin needle biopsy samples from 
fragmenting during handling from gross through embedding.  Don't want to use 
sponges.

Thanks,

Donna Willis, HT/HTL (ASCP)
Anatomic Pathology Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465 office
ph. 214-725-6184 mobile
donna.wil...@baylorhealth.edu

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RE: [Histonet] processors

[Nails, Felton]

I would have to agree with Rene. I have had two VIP's that were replaced after 
they were over 20 years old and they both were still running. I currently have 
3 VIP 6's, thus far the only problem I've experienced was the fluid level 
sensor malfunctioned once. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, June 12, 2012 3:29 PM
To: Nancy Schmitt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] processors

At this moment, just like in automobiles, all tissue processor can give you a 
good service and your selection should be based on your budget.
Having said that, you ask for a comparison between 2, the Leica ASP300 vs. 
Sakura VIP6 and, again, price could decide but from the quality and durability 
VIP6 wins hands down, at least for me!
René J.



From: Nancy Schmitt nancy_schm...@pa-ucl.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tuesday, June 12, 2012 11:35 AM
Subject: [Histonet] processors

Hi Histonetters-

Is anybody using the ASP6025 from Leica? Pros and cons please.

Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6.

As always, Thank You for your help - it is highly appreciated.

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
205 Bluff Street
Dubuque, IA  52001




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[Histonet] RE: Not requiring HT Certification)

[Nails, Felton]

I studies at the famed AFIP while in the military and have two degrees and 
never thought I would stay in histology. 22 years later I am still in the field.
I don't think OJT is a bad route it has often generated some of our best techs 
but I am a firm believer that new techs need to go through a formal program or 
have a B.S. degree. I feel this way because most facilities require a degree to 
move up in positions of authority. I have a wonderful tech that went through a 
formal associates histology program but does not have a B.S. degree, my 
facility will not allow me to promote her to a assistant manager position. So 
if you are a young tech out there and you have dreams of moving up into a 
leadership position, get a B.S. degree in something. That is why med techs are 
held in higher regards and why most assistant director and director positions 
come from the clinical lab.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Friday, May 25, 2012 8:26 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Not requiring HT Certification)



Like everyone else, I was going to keep quiet, but I can't.
I am sensitive to those downgrading us who took the OJT route.
I did the OJT route, had a BS in Biology, tried for Veterinary School, but that 
wasn't so.  What do I do with all of this Science?  As part of my Pre-Med 
curricula had to take histology, loved it.  Had no idea that it was a paying 
field and such.  Had I known, I would have applied to a school as a backup.  It 
took me a while, but when I was hired as a tech, I did not excel quickly 
(Cheryl remember my mistakes), but I learned and had patient coworkers.  
Move on down the line several years and another coworker questioned my 
knowledge of the chemistry behind a stain, and all of a sudden all of my 
organic and biochem can running out of my mouth.  I didn't realize that I 
really knew all of that.  I had always felt a little disadvantaged because I 
did not get formal training through a school, just studied and passed the HT 
with the help of my coworkers (shout out to  LSU VetPath).  
There is nothing wrong with OJT for Biology majors.  They should have the basic 
background to understand the chemistries and processes behind why we do what we 
do.  With the modernization of technologies and procedures in the histo lab 
some formal education is needed.  There are many ways to receive this 
education, it can be online, or in person.  It all depends on the learner.
To overcome the stigma we should continue with some of the things that are now 
in place to stabilize the training of our successors (yes we all are going to 
have to retire one day).  A continued push for formal training, promotion of 
the field, professionalism by our colleagues, and respect from the customers 
(pathologists, patients, and gen lab personnel). 
In order to facilitate change for respect, we must first present a unified 
front.  
I know good techs with no certification, I know bad techs with certification.  
We all do.  
Last year I celebrated my 20th year in histo and never thought I would be where 
I am.  I never wanted to do research, and didn't like management (don't like 
telling grown folks what to do), but I love teaching.  It helps me to learn the 
theory behind what I do and apply it better.  There are so many people who can 
do histo whether it is routine, special procedures, or management.  
We shouldn't look down on those who took the OJT route, sometimes they just 
don't know about a formal program (like me).  You never know where the next 
great manager, director or tech is coming from, so don't count them out.


Toysha N. Mayer, MBA, HT (ASCP)
Instructor, Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnma...@mdanderson.org




--

Message: 1
Date: Thu, 24 May 2012 17:20:41 +
From: joelle weaver joellewea...@hotmail.com
Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)
To: jkr...@deltacollege.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID: snt135-w63f3ac88ed033046fe696ad8...@phx.gbl
Content-Type: text/plain; charset=iso-8859-1


Jon There is a route with associates and training I believe. 
Of course I can't speak for the BOC, and I am sure that you want to help your 
employees as much as you can. I do see your point about the similarities in 
tasks. My thought would be that the exam eligibility states that they have to 
have recent experience in fixation, embedding, microtomy, and staining 
(histology) and the associated theory knowledge. EM is on the exam study 
topics, but also with the theory/experience for all those routine histological 
techniques, is how I read it. Take a look at the exam outlines, that should 
give you an idea of the scope. Ascp.org get 

RE: [Histonet] Block Storage

[Nails, Felton]

This is a very sticky issue, when I setup inhouse labs I always present the 
argument that during inspections the lab that produces a report should have 
access to all test material which includes blocks and slides per the CAP 
checklist.
Ann trying approaching it from that angle. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo
Sent: Tuesday, March 20, 2012 6:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Block Storage

Does anyone know if a laboratory in NJ is required to keep the blocks they 
perform Technical component  on if they do not perform the professional 
componentor should they have the facility performing the professional 
component store them?  Who is ultimately responsible? Ann 
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RE: [Histonet] Block Storage

[Nails, Felton]

Rene I think this question is eluding to a Physician owned lab and who is 
responsible for storing the blocks not how long they are retained?


From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Wednesday, March 21, 2012 9:20 AM
To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; Nails, Felton
Subject: RE: [Histonet] Block Storage

Depending on the state regulations and the laboratory policies,blocks are 
stored during different periods of time before being discarded. I used to store 
them for 9 years.
René J.

--- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote:

From: Nails, Felton flna...@texaschildrens.org
Subject: RE: [Histonet] Block Storage
To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date: Wednesday, March 21, 2012, 9:00 AM

This is a very sticky issue, when I setup inhouse labs I always present the 
argument that during inspections the lab that produces a report should have 
access to all test material which includes blocks and slides per the CAP 
checklist.
Ann trying approaching it from that angle.

-Original Message-
From: 
histonet-boun...@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=histonet-boun...@lists.utsouthwestern.edu
 
[mailto:histonet-boun...@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=histonet-boun...@lists.utsouthwestern.edu]
 On Behalf Of Ann Angelo
Sent: Tuesday, March 20, 2012 6:58 PM
To: 
histonet@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=histonet@lists.utsouthwestern.edu
Subject: [Histonet] Block Storage

Does anyone know if a laboratory in NJ is required to keep the blocks they 
perform Technical component  on if they do not perform the professional 
componentor should they have the facility performing the professional 
component store them?  Who is ultimately responsible? Ann 
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[Histonet] RE: ThermScientific HistoScreens-printing quality problems

[Nails, Felton]

This is an issue with the cassettes not your printer or ribbons.
If you take a straight edge and put it up against the edge of the cassette you 
will see that he cassette is concaving causing the styles to loose contact with 
the cassette.
It is more of a problem at the middle top edge of the cassette. It is only with 
the histoscreen cassettes and I bet Thermo is telling you that this is the 
first they are hearing about this problem. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DeMint, Valerie 
S
Sent: Thursday, March 08, 2012 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ThermScientific HistoScreens-printing quality problems

Hi
 
I am looking for help with a cassette printing problem that we are 
experiencing.  We use the Thermo Histo Screen cassettes and the Surgipath  VCP 
2001 cassette printer.  Case numbers are quite smudged or missing completely 
when the batch has finished processing.  This is only happening to the 
HistoScreens because we also use a routine cassette from another manufacturer 
and there are no problems with print quality.  I have switched lot numbers of 
the printer ink ribbon and continue to have problems.  It is not happening with 
every cassette on the batch so I do not believe it to be the printer itself.  
Is any one else experiencing printing quality issues with these cassettes?
your help is appreciated,
Valerie DeMint, HT(ASCP)
Histology Lead
Waukesha Memorial Hospital
Waukesha, WI


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RE: [Histonet] pathology grossing stations

[Nails, Felton]

I agree with Joe, Mopec makes a very good product. In the last 4 years I have 
purchased 6 MB6000 and our pathologist are very pleased with them, especially 
the absence of high noise levels and adjustability. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Thursday, December 08, 2011 5:05 PM
To: Bob Richmond; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] pathology grossing stations

Bob,
 try Mopec. When I was at this year's NSH in Cinci, they had a few different 
sizes and configurations.

Joe the Toe
- Original Message -
From: Bob Richmond rsrichm...@gmail.com
To: Histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Thursday, December 08, 2011 2:19 PM
Subject: [Histonet] pathology grossing stations


A laboratory I'm doing some work for is planning to remodel within two
 months. We see about 5,000 specimens a year, with a moderate number of
 hospital major specimens including placentas. Currently we have no
 grossing station, and this may be an opportunity to get one. It will
 be installed in a windowless unventilated room, like our present sink
 setup. I don't know the dimensions of the space available. I suspect
 the decision will be made on the basis of price, with us having very
 little input.

 What currently available grossing station do you have? Do you like it?

 Bob Richmond
 Samurai Pathologist
 Knoxville TN

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RE: [Histonet] Histo Aide Duties

[Nails, Felton]

This sounds like union rules not CAP rules 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, December 07, 2011 9:55 AM
To: histonet@lists.utsouthwestern.edu; 
histonet-requ...@lists.utsouthwestern.edu; Matthew Lunetta
Subject: Re: [Histonet] Histo Aide Duties

Embedding NO
Cutting ABSOLUTELY NO
Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) 
Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels 
to hand written slides if the slides are not pre-written Filing slides and 
blocks YES Matching slides with paper work YES Delivering slides to 
pathologists YES Arranging blocks/slides to cut YES Anything that does not 
involve a technical skill, training or certification.
A good laboratory aid doing all the above mentioned tasks can improve the 
histotechnologists productivity 2.5 times René J.

--- On Wed, 12/7/11, Matthew Lunetta mlune...@luhcares.org wrote:


From: Matthew Lunetta mlune...@luhcares.org
Subject: [Histonet] Histo Aide Duties
To: histonet@lists.utsouthwestern.edu, histonet-requ...@lists.utsouthwestern.edu
Date: Wednesday, December 7, 2011, 8:01 AM


Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified 
histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and 
what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)

-Inline Attachment Follows-


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RE: [Histonet] Statistical Information for GI Bx

[Nails, Felton]

The patient condition should dictate what is taken, so the number will vary. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON
Sent: Friday, November 04, 2011 6:12 AM
To: Histonet
Subject: [Histonet] Statistical Information for GI Bx

Does anyone know if and where I can find any statistical information on the 
average number of GI biopsies taken per patient? 


THANK YOU,
 
PATTI RUBEN-NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761
nelsonr...@verizon.net
 
 
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[Histonet] Leica Bond III

[Nails, Felton]

I want to poll Leica Bond III users to see if they are able to successfully run 
EBER Insitu on their unit?
We continue to get inconsistent staining and inconsistent background staining.
I have been told it's a pH problem, it's a tissue processing problem but we are 
able to do the stain with great results by hand. 
I welcome any and all suggestions  


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[Histonet] HM525 Cryostat

[Nails, Felton]

 
I purchased a new Microm HM525 cryostat about 3 months ago.
The unit overheated causing some of the internal parts to warp.
Has anyone else experienced this or similar issue?

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[Histonet] Bond III

[Nails, Felton]

We have been using the Bond III for about 4 months and have starting 
experiencing staining on our negative control slide.
We inserted and additional blocking solution and it help for the IHC stains but 
we are still experiencing the problem the with insitu.
Has anyone experienced this problem before and how did you solve it? 

Thanks, Felton
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[Histonet] RE: Slide Labelers

[Nails, Felton]

 I use a clipped corner slide from Statlab and we do not experience any 
problems.
(IJL-6110)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of karlisch, 
patricia
Sent: Wednesday, July 06, 2011 10:32 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Slide Labelers


Is anyone else out in histoland having issues with the Leica Slide labeler not 
printing 'permanently' on their  recommended slides.   We are using the 
recommended clipped corner, extra adhesive slides for our HE but every so 
often the print rubs off after a couple of days.   The slides then need to be 
paper labeled.   I am afraid that over time the slides will not be readable.
Pat

Patricia Karlisch
Supervisor, Histology
Pathology and Laboratory Medicine
Penn State Hershey Medical Center
500 University Drive, PA 17033
Tel:  717-531-6072
FAX  717-531-7741
pkarli...@hmc.psu.edumailto:pkarli...@hmc.psu.edu



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RE: [Histonet] Sakura Tissue-tek Glas Air bubble problems

[Nails, Felton]

If you are using one of the older film coverslippers there is a roller 
following the application of the film.
Make sure that roller is still in the correct place or that one of the springs 
is not missing or in need of replacing.
The job of that roller is to squeeze the air bubbles from under the film. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lees, Sara
Sent: Tuesday, May 17, 2011 9:18 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems

Hi All,

I am having horrible problems with air bubbles , can anyone help I have changed 
the speed increased the mountant volume, clean it, kissed it,  u name it and 
nothing has worked...Can anyone help

 
Thanks Sara
 
 


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RE: [Histonet] ASCP Route 2 HT Certification

[Nails, Felton]

Your best answer will come from ASCP so contact them directly. The number 
should be on the application 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez
Sent: Wednesday, May 04, 2011 4:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ASCP Route 2 HT Certification

Hi,

I was hoping someone could help me with the following questions:

1) What happens to the money if I were to submit my transcript and my fees for 
the exam and end up not qualifying? Do I lose my money or is it credited for 
when I do qualify?
2) How do I determine what classes are considered BIO and Chem? Does the prefix 
for the course ID have to contain BIO or Chem?
3) Can the courses taken be similar? Example: School A: I took Chem10: Intro to 
Chemistry  School B: Chm:3 Chemistry basics. If these classes are 
interchangeable between schools do they still count or does it have to be a 
more difficult chemistry course for it to qualify?
4)Is there anyway to check if you do qualify before paying exam fees?
5)Do you have to pass the class with a C or higher? I took a BIO class years 
ago and received a D, but I was given the credited hours for the course.


Route 2 for HT certification for ASCP
At least 60 semester hours (90 quarter hours) of academic credit from a 
regionally accredited college/university, with a combination of 12 semester 
hours (18 quarter hours) of biology and chemistry, or an associate degree from 
a regionally accredited college/university, with a combination of 12 semester 
hours (18 quarter hours) of biology and chemistry, AND one year full time 
acceptable experience in a histopathology (clinical, veterinary, industry or 
research) laboratory in the U.S., Canada or an accredited
laboratory* within the last ten years.

Thanks,
Eric
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[Histonet] RE: grossing stations

[Nails, Felton]

We switched from the gross lab senior because of premature rusting, with the 
Sakura demo our pathologist thought it was too loud.
We selected the Mopec MB600 and they have been very pleased.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Tuesday, April 26, 2011 7:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] grossing stations

We are looking into new grossing stations.   Can anybody give me their opinions 
on which to choose, experiences, and what to avoid?


1.  Mopec's Grossing Stations

2. ThermoFisher  Gross Lab Senior

3. Accu-Edge Workstations by Sakura

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046



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[Histonet] RE: Slides to Pathologist

[Nails, Felton]

100% is unrealistic unless your staff never calls in sick, take vacations, your 
lab never gets research projects that are needed today and you are always fully 
staffed. Realistic reside between 90 and 95% which is obtainable but not overly 
easily obtainable 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, March 11, 2011 3:39 PM
To: Histonet
Subject: [Histonet] FW: Slides to Pathologist

I received this question from my Quality Manager today.

 Hi Joyce,
What do you consider good performance regarding this?  I need some sort of goal 
for our lab key control points chart.
I could only find one reference on the internet from Presbyterian Hospital in 
Charlotte, NC from 1993 (yuck).  Their goal was 90% of the slides on time which 
they correlated to 2 days or less late slides per month. Their goal was also to 
have all the slides out by 11am..

Could I get feedback from you guys about what you expect for this PI goal? I'd 
like 100%, but sometimes staffing just doesn't stretch far enough!!

Thanks,

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
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RE: [Histonet] DAKO Artisan Kit Issues

[Nails, Felton]

We have experienced similar issues, however you can just call DAKO and they 
usually will replace it.
It is important that you call them so they will be aware of the problem and can 
pull that lot # if needed. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
mizly...@frontier.com
Sent: Monday, March 07, 2011 1:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAKO Artisan Kit Issues

Fellow Histologists:

This is my first time using Histonet but I need to ask if anyone has started 
having problems with their DAKO Artisan kits recently? We have started having 
leaking kits for Gram Lot # 10043062 and Expiration Date 4/30/2011, and plunger 
issues with the Iron Lot # 10047616 Expiration Date 10/31/2011. We have also 
found that the GMS kits Lot # 10049354 and Expiration 11/30/2011 we have 
received in the last two shipments are from the same lot but they do not work 
with our validated protocol. 
What is strange is that the previous GMS kit worked on the same control that we 
are using now, but does not work with the new kit, but when stained with 
PAS-Fungus we see the same fungus as we saw with the previous GMS kit. I think 
it could be something with the shipping method but wanted to see if anyone else 
has seen a difference.

Thanks

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[Histonet] Mouse staining for ATP

[Nails, Felton]

 I am looking for a reliable method for staining mouse muscle with ATP'ase pH 
4.3, 4.6 and 9.4.
Any help will be greatly appreciated.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Wednesday, February 09, 2011 9:05 AM
To: 'Erin Sarricks'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] PAS with Diastase Digestion

Spit  for 5 minutes  old school


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks
Sent: Wednesday, February 09, 2011 7:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS with Diastase Digestion

Hi Histonet-



I recently ran a PAS/D stain and had some issues with it.  Both with and 
without slides came out looking the same so I'm guessing my digestion step 
didn't work!  I used a Malt Diastase solution (0.5g to 500mL water) for my 
digestion.  This is the procedure I used:



1.Deparaffinize and hydrate to water.

2.Place the sections labeled “with” in diastase solution preheated to
37˚C for 1 hour.  Hold the sections labeled “without” in distilled water.

3.Wash in running water for 5 minutes

4.Place all section (with and without) in 0.5% periodic acid solution
for 5 minutes

5.Wash in 3 changes of distilled water

6.Place in Schiff reagent for 15 minutes

7.Wash in lukewarm tap water for 5 minutes (immediately sections turn
dark pink color).

8.Counterstain in Mayer’s Hematoxylin for 3 minutes.

9.Wash in tap water for 10 minutes

10.   Dehydrate starting with 95% ETOH, clear, and coverslip.



I am wondering if my solution possibly got too warm in the oven and hindered 
the enzyme activity, or is it possible I left it in too long?  Any tips would 
be much appreciated!  Oh, and I have about 300 slides to stain, so spitting on 
them is my last last last resort!  Haha!  Thanks in advance for all your help!







Erin Sarricks, HT (ASCP)

Histology Laboratory Technician

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarri...@us.army.mil
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[Histonet] RE: Hematology stainers

[Nails, Felton]

I thought I was the only histology lab that got stuck staining bone marrow 
smears.
I started with staining smears manually, then went to the hematek and finally 
discovered the Wescor stainer. The wescor stainer has really brought 
consistency to this area. The only potential problem is you have to keep up the 
maintenance, which is not extensive. I highly recommend the wescor and it is 
very nicely priced.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Monday, January 31, 2011 12:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hematology stainers

Hi Histonetters, do any of you out there stain the peripheral, aspirate and 
touch prep slides of bone marrows in the histo lab?  If you do, do any of you 
use an automated stainer and if so, what kind of stainers are being used?  I 
have quotes for a Wescor Aeorspray Stat Slide stainer, a QuickSlide Plus 
Automated stainer and a Hematek 2000.  If anyone has had experience good or bad 
for any of these stainers I would love to get your feedback.

 

Thanks,

Joanne Clark, HT

Histology Supervisor

Pathology Consultants of New Mexico

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[Histonet] RE: Looking for a product

[Nails, Felton]

I think I recall that statlab or mercedes medical carried that item. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan
Sent: Tuesday, January 18, 2011 11:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Looking for a product

Hi Everyone,

I am looking for a product call mini fan pads.  They are a pad that will 
neutralize formalin.  I can't remember who sells them.  Any help would be 
appreciated.  Last time I saw them they came like a roll of paper towels.

 

Thanks in advance,

Kaye Ryan

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[Histonet] RE: What New Tissue Processor Should we get ?

[Nails, Felton]

As have you, I have also had great service from my VIP's and plan on purchasing 
the VIP 6. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne
Sent: Monday, December 27, 2010 3:16 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] What New Tissue Processor Should we get ?

Hi All,
  If given the choice- what would be the best Tissue Processor to get for a 
small Path Lab.  We have always had VIPs. Ours is about 16 y/o and we need to 
replace it and use it as a back up or for small biopsies. I was looking at the 
new VIPs and our medical repair people are very happy w/ the VIPs in the past.  
I am looking at VIP 5 and 6.  Do not need anything too fancy.  Just want a 
workhorse that has little or no issues and is user friendly. 

Any input is much appreciate on these or other products - private emails great !

Thanks,

Helayne Parker
hpar...@skaggs.net
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[Histonet] RE: Special Stain Automation

[Nails, Felton]

The problem with the Nexus is that you can not do silver and AFB on the same 
run.
We use the Dako artisan with very consistent results and it is very easy to 
use. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond
Sent: Wednesday, December 01, 2010 9:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special Stain Automation

We are in the market for a new stainer to do special stains.  We currently are 
using the Nexus and have been unhappy with it, particularly with the silver 
stains for which we do a lot of.  I'm wondering what techs out there recommend 
for us to check into.



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positive impact in the lives and health of persons and communities by providing 
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Benedictine and Presentation Sisters.
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[Histonet] C3D IF

[Nails, Felton]

Is anyone perform immunofluorescence for C3D?

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RE: [Histonet] Paraffin Tissue Crumbles

[Nails, Felton]

I agree most of your times are too long, but you can still get sections if you 
put water on your ice block and allow the tissue to rehydrate a bit or put a 
little ammonium hydroxide and water on your ice and sit your blocks to be 
section in this solution. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker
Sent: Wednesday, November 10, 2010 9:17 AM
To: Michael Mashore; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Tissue Crumbles

Having some experience processing and embedding rodent tissues myself (by 
hand), I would say that you are over-dehydrating the tissues. Try cutting back 
the alcohol incubation times to 30 min or even 10 min each.

Regards,
Merced

--On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore 
mmash...@vapop.ucsd.edu wrote:

 Hello Histonet Users,



 I have just started using paraffin and am having many difficulties. 
 Most of the time my tissue crumbles when sectioning. I have no real 
 experience in paraffin histology and have been given the task of 
 becoming proficient by myself, so I am hoping for feedback as to why 
 my tissue keeps crumbling. The tissue in question has been:  skeletal 
 muscle, cardiac muscle, liver, and brain (all from rat).



 The tissue was fixed in 10% neutral buffered formalin for 7 days at 
 4°C and then transferred to an automated tissue processor, with the 
 following
 schedule:



 2 hours 70% dehydration alcohol

 2 hours 80% dehydration alcohol

 2 hours 95% dehydration alcohol

 2.5 hours 95% dehydration alcohol

 2 hours 100% dehydration alcohol

 2 hours 100% dehydration alcohol

 2 hours 100% dehydration alcohol

 .5 hour Hemo-De

 .5 hour Hemo-De

 .5 hour Hemo-De

 1 hour paraffin

 4 hours paraffin



 They were infiltrated for 1 hour without vacuum then embedded.

 The blocks were stored in the freezer before cutting.

 The knife angle was 5°.

 Sections were 5µm thick.



 I would appreciate any feedback whatsoever.



 Thank you very much.



 Michael

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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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[Histonet] RE: Medite Microtomes

[Nails, Felton]

I have had some experience with this company and their embedding station but 
not microtome. The small cold area where you orient the tissue tends to ice 
over. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Fields
Sent: Saturday, October 30, 2010 4:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Medite Microtomes

Hi Netters,

Does anyone out there use the Medite Microtomes?  If so, have you had any 
problems with them?  I purchased 3 of them less than a year ago and I have 
already sent them back to Germany because of advancing (back and
forth) issues.  They are telling me (of course) no one else has had these 
issues and they want to send me a third set of them (the 2nd set are demos that 
are having some of the same issues).  I would appreciate any feed back on their 
microtomes and company.
Thank you in advanceI'm afraid I have made a big mistake in buying these.
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com



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RE: [Histonet] Marking inks

[Nails, Felton]

We use marking ink from Statlab or Mercedes Medical.
After applying the ink to the tissue, we take weak acetic acid solution and 
blot it over the ink which helps it adhere. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Friday, October 01, 2010 9:41 AM
To: Histonet
Subject: [Histonet] Marking inks

What are labs using for marking inks for surgical pathology specimens?  I 
have been told that we are having problems with the product that we are 
currently using.  Thanks.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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RE: [Histonet] RE: Cutting, Processing, etc

[Nails, Felton]

As I look through and monitor questions, it is apparent that our field is 
declining. These are very basic questions not about special stains or IHC 
stains but basic histology that should have been taught in histology 101. My 
fear is that as we get older and leave the field, who and what will be left to 
carry the torch. Those of you who ask, don't take offense to my thoughts but 
take action and pick up a book and read. You will improve yourself and the 
field.
Just my thoughts, if I offended you it was not my intent. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Friday, September 17, 2010 11:42 AM
To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Cutting, Processing, etc

My first reaction to the what is happening to our field, was WOW.  It seemed 
unkind to me, as if they original poster should not have asked these questions. 
 With further reading of the replies to this post, I am not so sure it was an 
unkind response, but one of potential shock and dismay to the idea that labs  
might not be producing the quality work that most of us employ on a daily basis.

Amy, in answer to your questions, I will echo some of the sentiments that I 
have read here.

1. Facing of blocks.  We use one blade to face blocks and another, new blade 
when we do our actual sectioning.  In my case, I face as many as I can, knowing 
I am going to toss that knife when I am done facing.

2.  Soaking of blocks.  After facing my blocks, I will put them on a cold, 
damp, ice cube tray.  This will achieve two purposes for me, a) to chill the 
block and b) to introduce moisture into the faced tissue.  If I get a block 
that is particularly dry or hard (some calcified tissues for example), I will 
face them, put them face down on my waterbath and allow the hot water to 
penetrate into the tissue for 15-45 seconds, depending on the block.
After cooling on the ice tray, they usually cut like butter for me.
Typically, my blocks are not on the ice cubes for more than 15 minutes.  As I 
cut some, I will rotate the blocks around the ice tray, adding more warm
blocks for cooling.

3.  Freeze spray.  I hardly ever use the freeze spray.  About the only time I 
find that I need it is if I have a particularly fatty tissue and it doesn't 
want to section.

4.  Tissue processor changes.  This is definitely something that is site 
specific. In our case, we do base it on volumes.  If we have a small volume of 
our little biopsies, we might not change the machine weekly, but every two 
weeks.  Generally our large specimen machine is changed weekly.

Your mileage may vary!  :o)

Michelle





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el 
razik
Sent: Friday, September 17, 2010 5:38 AM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Cutting, Processing, etc


i think that histonet is a primary educational group for all levels and any 
expert in our feild have asked these quistions one day befor and we should ask 
freely without any shame . i'm as begainner have learned alot from these little 
quistions. and i asked befor for name of antibodies and its use to be written 
to clear the information for all levels and no shame for that.

--- On Thu, 9/16/10, Nails, Felton flna...@texaschildrens.org wrote:


From: Nails, Felton flna...@texaschildrens.org
Subject: [Histonet] RE: Cutting, Processing, etc
To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu
Date: Thursday, September 16, 2010, 6:06 PM


what is happening to our field??

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R
Sent: Thursday, September 16, 2010 10:38 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cutting, Processing, etc

Hello Histoland!



I have some questions about procedures in different histo labs and I'd like to 
have some 'backup' when people look at me like I'm crazy here...



After embedding, you face (trim) your blocks, right? Do you take sections right 
from that same blade, or move/change your blade?

How many of you 'soak' your blocks in water/softblock before cutting them?
Do you put them on a cold plate/use ice and water, etc? Does this depend on the 
type of tissue, or do you treat them all the same?



How often is freezy spray used in your lab, and where and when do you use it?



How often do you rotate/change your reagents in your processors? Do you 
calculate this by how many blocks/days/weeks of use?



Thank you so much for your input!!



Amy Senn, HT

Holy Spirit Hospital, Camp Hill PA





















Attention:  This Message is intended only for the use of the individual or 
entity to which it is addressed, and may

[Histonet] RE: Cutting, Processing, etc

[Nails, Felton]

 what is happening to our field??

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R
Sent: Thursday, September 16, 2010 10:38 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cutting, Processing, etc

Hello Histoland!

 

I have some questions about procedures in different histo labs and I'd like to 
have some 'backup' when people look at me like I'm crazy here...

 

After embedding, you face (trim) your blocks, right? Do you take sections right 
from that same blade, or move/change your blade?

How many of you 'soak' your blocks in water/softblock before cutting them? Do 
you put them on a cold plate/use ice and water, etc? Does this depend on the 
type of tissue, or do you treat them all the same?

 

How often is freezy spray used in your lab, and where and when do you use it?

 

How often do you rotate/change your reagents in your processors? Do you 
calculate this by how many blocks/days/weeks of use?

 

Thank you so much for your input!!

 

Amy Senn, HT

Holy Spirit Hospital, Camp Hill PA

 

 

 

 

 

 

 



 

 

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[Histonet] RE: Pathology billing for consultation

[Nails, Felton]

That is the case here also 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A
Sent: Wednesday, September 08, 2010 10:37 AM
To: 'Weems, Joyce'; Demarinis, Carolyn; histo...@pathology.swmed.edu
Subject: [Histonet] RE: Pathology billing for consultation

If our Pathologists need a second opinion we eat the charge.  If it is 
requested by the clinician, we request that he/she be billed.   If it is the 
patient we bill the patient (or ask the consultant to bill the patient)  All of 
this depends on patient status/medicare etc.
I'm very interested in seeing how others respond.
Jan Mahoney
Omaha

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Wednesday, September 08, 2010 9:46 AM
To: Demarinis, Carolyn; histo...@pathology.swmed.edu
Subject: [Histonet] RE: Pathology billing for consultation

It is my understanding that we cannot charge professional fees for other 
pathologists. Our hospital pays for the pathologists' consults if the patient's 
insurance will not or if the consultant does not bill third party.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Demarinis, 
Carolyn
Sent: Wednesday, September 08, 2010 09:22
To: histo...@pathology.swmed.edu
Subject: [Histonet] Pathology billing for consultation

I would like to know how other pathology labs are billing for consultations 
that are sent out by pathologist for second opinion.
Our process is to notify the physician's office that a case is being sent to an 
expert and, if required, the physician's office is responsible for obtaining 
precertification if the patient's insurance require it.
Unfortunately, this has caused us a number of problems.  If the consultant is 
not in-network, the insurance does not cover this expense, and the patient is 
responsible for the bill.
Is it a better option for the hospital to receive all bills from consultants, 
and in turn, the hospital will bill the patient?  If so, are there problems 
associated with this?
Or are other laboratories having the consultants bill the patient's insurance 
directly, and if so, are they experiencing similar problems?
Thanks.
Carolyn DeMarinis, Pathology Supervisor
Saratoga Hospital Laboratory




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[Histonet] RE: embedding center just did alot of research!

[Nails, Felton]

Even over Tissue Tec 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph
Sent: Thursday, September 02, 2010 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding center just did alot of research!

I did a lot of research on embedding centers and made the mistake of going with 
the least expensive new one.  If you want to know what I bought email me 
separately.  The prices are in the stratosphere but I have to say Leica still 
has the edge for this 30 plus year tech.

 

Nick Madary, HT/HTL(ASCP)QIHC

Histology Mgr, Medimmune

301.398.6360(lab), 4745(vm),9745(fax)

 

To the extent this electronic communication or any of its attachments contain 
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the sender advising of the error in transmission and delete the original 
message and any accompanying documents from your system immediately, without 
copying, reviewing or otherwise using them for any purpose.  Thank you for your 
cooperation.

 

 




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[Histonet] Immunofluorescence QC

[Nails, Felton]

We have always done IF's utilizing internal positive controls. The new CAP 
checklist is now requiring positive and negative controls. What are you guys 
doing for negative controls? 

 

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RE: [Histonet] Tonsils submission for pathology

[Nails, Felton]

Tonsils are gross only unless there is a difference of 5cm between the two 
tonsils. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Tuesday, August 31, 2010 12:50 PM
To: Histonet
Subject: [Histonet] Tonsils submission for pathology

What are your guidelines for submission of pediatric tonsils to pathology?  
Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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RE: [Histonet] accu-edge low profile microtome blades

[Nails, Felton]

It's not the oil that's giving you trouble because they have always had oil 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins
Sent: Monday, August 30, 2010 3:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] accu-edge low profile microtome blades

Hello all,

We use the accu-edge low profile microtome blades (exclusively, as they work 
best for us).  We noticed an oil/lubricant of some sort on the blades in the 
last box that we opened (we checked one other box and it has the same, although 
none previously did, or at least we didn't notice it).  The oil is giving us 
problems with our sectioning.  Has anyone else noticed this, either now or in 
the past?

Also, does anyone have a suggestions of other blades we should use?

Thanks for your input,
Brandi Higgins, BS, HT(ASCP)
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RE: [Histonet] Tissue-Tek VIP 2000-3000

[Nails, Felton]

Agreed extremely reliable 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 13, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu; Mike Tighe
Subject: Re: [Histonet] Tissue-Tek VIP 2000-3000

I don't know the answer to your first 2 questions, but reliable they really are!
René J.

--- On Fri, 8/13/10, Mike Tighe mti...@trudeauinstitute.org wrote:


From: Mike Tighe mti...@trudeauinstitute.org
Subject: [Histonet] Tissue-Tek VIP 2000-3000
To: histonet@lists.utsouthwestern.edu
Date: Friday, August 13, 2010, 4:26 PM



I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these 
units still serviceable? Is it hard to find parts? Are they Reliable? 

Thanks for any help!!
Mike


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[Histonet] Sakura Tape coverslipper

[Nails, Felton]

 
Has anyone been experiencing a brown cornflaking artifact on their slides after 
being coverslipped.
I was told by Sakura that it was do to the slides drying out when transferred 
from the stainer to the coverslipper.
Which can't be correct because on the new sakura coverslipper there is a 
holding area which is filled with xylene so if the tissue dried out it would 
rehydrate before being coverslipped. 

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[Histonet] RE: inking dyes

[Nails, Felton]

Statlab or TBS 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Thursday, July 15, 2010 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] inking dyes

Can someone provide me with a supplier of tissue marking dyes for use on the 
grossing bench.
 
Diana McCaig
Histology Lab
Chatham Kent Health Alliance
80 Grand Avenue West
Chatham. Ontario
N7L 1B7
519-352-6401  (6604)
 
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[Histonet] RE: Cap Question: 2 identifiers for slides

[Nails, Felton]

Not yet 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, July 01, 2010 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cap Question: 2 identifiers for slides

Is is a CAP requirement to have 2 identifiers on surgical slides?

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
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[Histonet] PBA for Immunofluorescence

[Nails, Felton]

Concerning Immunofluorescence, where are you getting your (PBA) Protein 
Blocking Agent from? I use to order it from Thermo Shandon but they no longer 
carry it. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Thursday, May 20, 2010 1:49 PM
To: Laurie Colbert; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Mounting Medium for Immunofluorescence

I think that the propidium iodide will stain the nuclei or DNA, similar to 
DAPI, so this mounting media will counterstain the nuclei and its visible in 
the FITC wavelength (488), while if you conterstain with DAPI it is excited 
with a different wavelength, therefore it might interfere with the FITC portion 
of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 
18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent aqueous 
mounting medium.  By mistake, today they were coverslipped with a mounting 
medium that we use for coverslipping our FISH slides.  This mounting medium is 
described as Mounting medium for fluorescence, with propidium iodide.

Will this make a difference when reading the slides??

 

Laurie Colbert

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[Histonet] RE: [Derm procedure

[Nails, Felton]

Would anyone out there in a Derm Lab be willing to share their procedure used 
to process tissue?


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of thisis...@aol.com 
[thisis...@aol.com]
Sent: Sunday, May 02, 2010 5:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Full Time 4-day, 40 hr position in NJ

 We are a reference lab in central NJ looking for a 'seasoned' Histotech to 
work four 10-hr days, Tues - Friday, 8am -6:30pm in our very busy Histology 
laboratory.  Job duties include:  Grossing, embedding, cutting, staining 
(Ventana platform for both HE and IHC) coverslipping, etc,.)  Experienced 
Techs Only.
Please e-mail your resume to thisis...@aol.com if you are interested.




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RE: [Histonet] Slide and Block Storage

[Nails, Felton]

If you are supplying the professional component, then it is a good idea to 
maintain the blocks and slides.
If you are inspected and they do an audit trail you must be able to produce the 
slides and/or blocks. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams
Sent: Thursday, April 29, 2010 11:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and Block Storage

My laboratory provides professional services to some clients and technical 
services to others.  The question has recently come up, who should store blocks 
and slides when the pathologist and histology laboratory are different 
companies?

- Dan

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RE: [Histonet] Slide and Block Storage

[Nails, Felton]

 I am able to reply to questions n the histonet but I am unable to post 
anything on the histonet. Is there a different address I should be using 
instead of histo...@list.utsouthwestern.edu?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Friday, April 30, 2010 7:12 AM
To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide and Block Storage

If you are supplying the professional component, then it is a good idea to 
maintain the blocks and slides.
If you are inspected and they do an audit trail you must be able to produce the 
slides and/or blocks. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams
Sent: Thursday, April 29, 2010 11:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and Block Storage

My laboratory provides professional services to some clients and technical 
services to others.  The question has recently come up, who should store blocks 
and slides when the pathologist and histology laboratory are different 
companies?

- Dan

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RE: [Histonet] Slide and Block Storage

[Nails, Felton]

So what if the reporting company need to order special stains, it adds too much 
time having to request the blocks or additional unstained slides. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Kronenberger
Sent: Friday, April 30, 2010 9:40 AM
To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide and Block Storage

If our lab does slide preps for other clients (Technical component only), we 
hold the blocks at our facility.  The slides are read and reported by the 
client, they hold the slides.

Liz

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams
Sent: Friday, April 30, 2010 12:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and Block Storage

My laboratory provides professional services to some clients and technical 
services to others.  The question has recently come up, who should store blocks 
and slides when the pathologist and histology laboratory are different 
companies?

- Dan

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RE: [Histonet] (no subject)

[Nails, Felton]

It really depends on your volume to be processed and what you are planning to 
process. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila
Sent: Monday, April 26, 2010 10:10 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

I am looking into purchasing a microwave tissue processor. 

I would be interested to hear from people who has had experience with microwave 
tissue processing. And what brands would you recommend?

Thanks a lot,

Lucy

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RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines

[Nails, Felton]

What do you process and don't measure, so everything is grossing. Even our 
foreskins which are GDO, we take a measurement in our dictation. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer
Sent: Friday, April 02, 2010 11:41 AM
To: Janice A Mahoney; kim.dona...@bhcpns.org; 
histonet-boun...@lists.utsouthwestern.edu
Cc: Histonet; 'Joe Nocito'
Subject: RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines

Just talked to Jim at CAP and he said the term Processing still can be the 
transfer of tissue from one container to another (cassette) to be processed in 
the tissue processor.  If there is any analytical thinking involved (dying 
margins, measuring...) the process becomes Grossing and falls under the high 
complex rule.

Hope that helps.



Kathy Boozer, HT (ASCP), IHCQ
Adventist Medical Center
10123 SE Market St.
Portland, OR  97216
booze...@ah.org

 


 kim.dona...@bhcpns.org 04/01/2010 09:15 
I agree Janice. The only thing it looks to me is to now define Grandfathered 
in. Because it also states in addition for that criteria as well. 


Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Mahoney,Janice A janice.maho...@alegent.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu 
03/31/2010 04:10 PM

To
'Joe Nocito' jnoc...@satx.rr.com, Histonet 
histonet@lists.utsouthwestern.edu
cc

Subject
RE: [?? Probable Spam]  [Histonet] New CAP grossing guidelines






But above that after the education piece it says in Addition.
Jan, Omaha

-Original Message-
From: Joe Nocito [mailto:jnoc...@satx.rr.com] 
Sent: Wednesday, March 31, 2010 4:08 PM
To: Mahoney,Janice A; Histonet
Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines

just had a lively discussion at work. My take is that the only thing CAP
changed was that they combined the processing and grossing pieces
together again, which I don't know why they split them in the first place.
But you don't have the entire CAP note and many people miss this.  The 
last
item states OR three months of documented laboratory training in the high
complexity area. Again, my take is that an unregistered histotech can have
at least three months of documented training in grossing complex 
specimens,
have the record signed off by the medical director and be ok. How far off 
am
I?

Joe
- Original Message -
From: Mahoney,Janice A janice.maho...@alegent.org
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Wednesday, March 31, 2010 2:44 PM
Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines


Is anyone concerned about the new (old) grossing personnel guidelines from
CAP.  Many labs use people to process  tissue.  No more!


ANP.11610 Phase II

If individuals other than a pathologist or pathology resident assist in
gross examinations, do such individuals qualify as high complexity testing
personnel under CLIA regulations?

NOTE: The laboratory director may delegate the dissection of specimens to
non-pathologist individuals; these individuals must be qualified as high
complexity testing personnel under CLIA regulations. The minimum
training/experience required of such personnel is:

 1.  An earned associate degree in a laboratory science or medical
laboratory technology, obtained from an accredited institution, OR
 2.  Education/training equivalent to the above that includes at least 60
semester hours or equivalent from an accredited institution. This 
education
must include 24 semester hours of medical laboratory technology courses, 
OR
24 semester hours of science courses that includes 6 semester hours of
chemistry, 6 semester hours of biology, and 12 semester hours of 
chemistry,
biology or medical laboratory technology in any combination. In addition,
the individual must have laboratory training including either completion 
of
a clinical laboratory training program approved or accredited by the 
ABHES,
NAACLA, or other organization approved by HHS (note that this training may
be included in the 60 semester hours listed above), OR at least 3 months
documented laboratory training in each specialty in which the individual
performs high complexity testing.

The CLIA regulations on high complexity testing personnel may be found at 
HC
Testing Personnelhttp://wwwn.cdc.gov/clia/regs/subpart_m.aspx.

In addition, the CLIA regulations include exceptions for grandfathered
individuals; these regulations (42CFR493.1489 and 1491) may be found at 
the
above Web address and at Grandfathered
Exceptionshttp://wwwn.cdc.gov/clia/regs/subpart_m.aspx.

It is the responsibility of the laboratory director to determine whether 
an
individual's education, training and experience satisfies the requirements
of this checklist question.
Jan Mahoney
Omaha, NE


Sponsored by Catholic Health Initiatives and Immanuel Health 

RE: [Histonet] RE: Leica Paraplast

[Nails, Felton]

There is about three brands of paraplast and they all cut differently. In one 
of my labs I use paraplast plus and it ribbons very well however the blocks 
have to be colder then if you are using TissuePrep from Fisher.
Leica may have sent you one of the other types of paraplast. (paraplast, 
Paraplast Plus, Paraplast Xtra) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of connie grubaugh
Sent: Tuesday, March 30, 2010 9:58 PM
To: ddree...@sbcglobal.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Leica Paraplast


Hi all, I questioned the Leica paraplast that we have received too.  It is real 
gummy and sticky.  Takes me forever to cut. I asked and was informed that it is 
the same stuff we have always got and there is no difference. Except all of us 
techs have noticed a big difference. 



Connie G.



 

 Date: Tue, 23 Mar 2010 11:29:03 -0700
 From: ddree...@sbcglobal.net
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] RE: Leica Paraplast
 
 
 Hi Kristen,
 We were using the Paraplast Xtra and switched to something else after we 
 noticed a difference in the quality of the paraffin. The tissues weren't 
 cutting as well and the paraffin seemed to be gritty. We found we were 
 going through many more blades due to nicks and scratches and many times had 
 to switch blades mid-block.
 
 
 From: histonet-requ...@lists.utsouthwestern.edu 
 histonet-requ...@lists.utsouthwestern.edu
 Message: 4
 Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT)
 From: kristen arvidson arvidsonkris...@yahoo.com
 Subject: [Histonet] Leica Paraplast
 To: histonet histonet@lists.utsouthwestern.edu
 Message-ID: 94596.93077...@web65707.mail.ac4.yahoo.com
 Content-Type: text/plain; charset=iso-8859-1
 
 Has anyone who uses paraplast (we use the basic one) noticed a change in the 
 quality of your tissue?  I have recently found out that they have changed 
 manufacturing sites in the past couple of months.  I am having on and off 
 issues with my skin specimens that have been going on for about 2 months or 
 so.  Thought there may be a correlation.  Any thoughts? 
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[Histonet] RE: coverslipper

[Nails, Felton]

It depends on your volume and how long you maintain your slides. Because you 
are a children's hospital you keep them longer.
The Leica brand is very troublesome in high volume situations. I personally 
prefer Sakura tape for the speed and fast drying time. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Thursday, March 25, 2010 11:15 AM
To: Margaryan, Naira; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] coverslipper

Leica CV5030.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Margaryan, Naira
Sent: Thursday, March 25, 2010 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] coverslipper


Hi Colleges,

I need your opinion about coverslip by hand vs. using machine.
If you use machine what company's coverslipper you prefer?

Thanks in advance,
Naira

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RE: [Histonet] coverslipper

[Nails, Felton]

Is this coverslipper now produced by Medite 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk
Sent: Thursday, March 25, 2010 2:40 PM
To: malbena...@gmail.com; 'Lynette Pavelich'
Cc: histonet@lists.utsouthwestern.edu; 'Naira Margaryan'
Subject: RE: [Histonet] coverslipper

I purchased a used Hacker RCM-3660 glass coverslipper several years ago.  It 
was missing some parts and the nice people from Hacker supplied me with the 
needed parts.  This work horse has been running 7 days a week for the past 
several years and has never given us any problems.  Great machine and great 
people.

Jim

___
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 www.masshistology.com
   www.nehorselabs.com
 
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Malika Benatti
Sent: Thursday, March 25, 2010 3:08 PM
To: Lynette Pavelich
Cc: histonet@lists.utsouthwestern.edu; Naira Margaryan
Subject: Re: [Histonet] coverslipper

We use the Leica CV5030 the advantage of it is that it can be attached to their 
Auto stainer providing continuous workflow as rack and coverslip 30 slides per 
run, without the need to physically transfer slides rack between the 
autostainer and coverslipper or used as a stand alone coverslipper if needed, 
though at time it can be problematic.

For a start glass cover slip must be kept at 37 oC prior use otherwise they 
tend to stick to each others.
Also does not recognised all the slides type unless they are Leica one, or the 
coverlslipper as been calibrated to use a specific slides type.

In the past I used the Sakura Acetate film coverslipper, and their were no 
major problem with it though remounting section was a very tedious process.

Malika





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RE: [Histonet] help

[Nails, Felton]

Basically time 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, March 23, 2010 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help

After you stop laughing seriously I need some help here, apparently I got my 
fingers in some silver nitrate yesterday and touched my face under my nose over 
my lip and now I have black spots that won't come off.  I have done this on my 
hands before but never on my face.  So far I have tried soaking a cloth in 
hydrogen peroxide hoping to bleach it with no luck, I even tried putting some 
gold chloride on it to see if I could tone it down, to no avail.  Any ideas?  
Make up only goes so far and lasts so long.

 

Regards,

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net

 


This email is confidential and intended solely for the use of the Person(s) 
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RE: [Histonet] Prostate Bx's

[Nails, Felton]

Why would you want to prelabel the containers prior to seeing the patient?
Outside of the kit being labeled LLB LLM LLA, etc., the individual containers 
should not be label until you actually add the sample to avoid mistakes. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of S R
Sent: Monday, March 22, 2010 9:26 AM
To: histo net
Subject: [Histonet] Prostate Bx's


Good Morning Everyone,

I was wondering for those of you working in POL labs or even hospitals, if you 
are pre-lableing specimen bottles before the patient comes in?  Ie 12 part 
prostate boxes have the patient's name and the bx site pre-made days or weeks 
before the patient comes in.  If not do you know of any documentation that 
states this should not be done.  I have been looking for something that says 
the bottles should not be pre-labeled before the patient is seen, but have 
unfortunatley have not been able to find any.  

thanks in advanced

sammy
  
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RE: [Histonet] Block patient IDs

[Nails, Felton]

It is my understand that the two patient identifier applies to the completed 
slide not the block.
You can verify this by looking at the CAP Checklist for anatomical pathology 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van 
Binsbergen
Sent: Friday, March 12, 2010 9:42 AM
To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Block patient IDs

A question for all you CAP fundis: how many patient Identifiers are needed on 
each paraffin block

We currently just write the unique, LIS generated number on the block face, but 
have recently been advised that CAP and JCIA require not one, but TWO patient 
IDS on the block

Comments please

--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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[Histonet] IHC

[Nails, Felton]

Joe, I wanted to ask you a question about which type of IHC stainer you are 
using and which stainer do you think is the user friendly and efficient.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Friday, February 26, 2010 4:51 PM
To: CHRISTIE GOWAN; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fire in the lab

Once upon a time in a far away land, we used to boil our embedding molds in 
boiling soapy water, over an open Bunsen burner, followed by an alcohol rinse 
then air dry. One time the fire alarm was activated and we had to evacuate the 
hospital. We were out there quit awhile. When we received the all clear to go 
back into the hospital, I was the first one back in the lab and the fire 
department was there, looking into our pot that had boiled out and was smoking 
up the lab. This wasn't the cause of the first alarm, but it did set off the 
second.

Joe
- Original Message -
From: CHRISTIE GOWAN christiego...@msn.com
To: histonet@lists.utsouthwestern.edu
Sent: Friday, February 26, 2010 8:20 AM
Subject: [Histonet] Fire in the lab





Dear Histonet Friends,

I just wanted to share an incident we recently had with an old paraffin pot. 
One of my techs came in on Sunday to embed some tissues, went into the 
processor room and smelled something burning. He noticed our old paraffin 
pot had charred looking labels on the outside so he went over, opened the 
lid and poof!!! the pot went up in flames. The thermostat had gone haywire 
and heated the paraffin to flash point. Opening the lid gave it the oxygen 
it needed to ignite. He triggered the alarm, made the appropriate call and 
then put it out with an extinguisher. Of course it kept re-igniting because 
he could not get behind it to pull the plug. The fire dept finally was able 
to get it pulled out and unplugged. Needless to say the tech was shaken and 
the room was a mess. I applaud his courage and am not sure I would have done 
the same. There was enough xylene and alcohol on the 4 processors to cause 
quite an explosion but everything else was in a flammable cabinet. I was 
wondering if this type of thing had ever happened to anyone else?? Needless 
to say, we have de-comissioned all old paraffin pots and will order only 
those with over temp safety features. I guess I just wanted to remind 
everyone that fires can happen in the lab and do probably more often than we 
hear about. This was the first time for me and I have been in this business 
for over 20 years. Take care and be safe.

Christie Gowan HT (ASCP)
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[Histonet] Sirius Red

[Nails, Felton]

 Is there a good Sirius Red procedure used to stain collagen
And where can I find the powder  
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RE: [Histonet] Sirius Red

[Nails, Felton]

Thanks for everyone's help


From: John Kiernan [mailto:jkier...@uwo.ca]
Sent: Wednesday, February 24, 2010 11:36 AM
To: Nails, Felton
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Sirius Red

You'll find brief instructions for the picro-sirius red method for collagen in 
nearly every histological techniques book published since about 1970. A Google 
search for sirius red vendor brings up 484 hits, though not all are for 
vendors. It's important to get the correct dye (C.I. 35780, Direct red 80). For 
a thorough discussion of how to use the method intelligently, see L. Junqueira 
et al 1979. Histochem. J. 11: 447-455.
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: Nails, Felton flna...@texaschildrens.org
Date: Wednesday, February 24, 2010 12:23
Subject: [Histonet] Sirius Red
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu

  Is there a good Sirius Red procedure used to stain collagen
 And where can I find the powder
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RE: [Histonet] Ultralight

[Nails, Felton]

Thanks Beth, I have not had a lot feedback on this question. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Millerman
Sent: Monday, February 15, 2010 1:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ultralight


Felton,

My lab is Dermatology research and processes skin samples only.  I have tried 
the UltraLight Fixative by Pacific Southwest and found that it works quite 
nicely .  My HE are picture perfect, and my receptors on my IHC's stain well. 
I use it because it doesn't seem to be as harsh or drying as 10% Formalin.

Beth Millerman/SWC
Scientist
Stiefel Laboratories, Inc,, a GSK company Direct Dial; 650-739-2982




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[Histonet] Ultralight

[Nails, Felton]

 
Have anyone in Histoland converted from 10% NBF to the Ultralight Histology 
system of processing?
I think it is marketed by Pacific Southwest Lab Equipment  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, February 12, 2010 9:46 AM
To: Jay Lundgren; histonet
Subject: RE: [Histonet] Distance learning?


 Darton College in Albany GA has an Associates Degree program. 
 
http://www.study-online.net/schools/darton-college/13439.htm
 
Contact: 
 
Carl Sagasser, BS, HT(ASCP)

Educational Coordinator

Histotechnology Program

Darton College

2400 Gillionville Road

Albany, GA 31701

P: (229) 317-6974

F: (229) 317-6682 

 

There is also a program at Indiana University - 
http://www.indiana.edu/~bltindy/ahlt/histotech/cert.html
 

Glenda Hood Hoye developed that program but she is no longer there. The web 
site above will give you some contact numbers if you want to check on it. 

 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Friday, February 12, 2010 10:25
To: histonet
Subject: [Histonet] Distance learning?


  A few years ago I remember hearing about a NAACLS approved, distance learning 
Histotechnology program.  They were using the Internet and teleconferencing to 
train students.  Is anyone still doing this, and could you tell me where?

 Thanks for your time,

   Jay A.
Lundgren, M.S., HTL (ASCP)
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RE: [Histonet] Peripheral Blood Smears

[Nails, Felton]

I have been fighting this battle for years and still have not been successful. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Thursday, February 11, 2010 8:44 AM
To: dkb...@chs.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Peripheral Blood Smears

You're a Histology Dept.! Get the peripheral smears out of your dept.
Peripheral smears belong in Hematology. Make then make something work.
This is what I did.
Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dkb...@chs.net
Sent: Thursday, February 11, 2010 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peripheral Blood Smears


We have had this discussion many times in our institute and would like some 
outside thoughts.  Is there a charge/cpt code that is acceptable for 
the technical aspect of a peripheral smear?   Hematology doesn't stain
our
slides.  They make the smear and we accession, stain and coverslip, etc.

But the pathologist is the only one actually interpreting the smear.
Thanks.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l
F: 
804-765-5582 l dkb...@chs.net






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RE: [Histonet] GI, Uro, or Derm Path Lab Set Up

[Nails, Felton]

Especially when these physician hire unqualified people to run these labs and 
flood the histonet with their uneducated questions.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Thursday, February 04, 2010 2:29 PM
To: histonet@lists.utsouthwestern.edu; Timothy Jay
Subject: Re: [Histonet] GI, Uro, or Derm Path Lab Set Up

We don't need anymore pathology laboratories.  What we need is support of 
existing laboratories, especially hospital-based labs.  GI and GU physicians 
are Cherry-picking the technical revenue that should be going to hospital 
labs.  Let's reform health care; make it more efficient and less expensive.  We 
don't need to be putting more money in clinicians' pockets.

Richard

Richard W. Cartun, Ph.D.
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

 Timothy Jay tja...@yahoo.com 2/4/2010 1:28 PM 
For those needing help putting an in-office path lab together whether you are 
GI, Urology, or Derm please send me an email at tja...@yahoo.com or call me at 
775-830-1591. I have a consulting business that specializes in putting these 
labs together. References provided upon request. 
 
Timothy Garcia-Jay, MHA



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RE: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2010 MEETING

[Nails, Felton]

Please forward me a copy.

Thanks, 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
kdwyer3...@aol.com
Sent: Saturday, January 30, 2010 12:23 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2010 MEETING




All Histonetters: 
The program is complete for the Texas Society for Histotechnology 2010 State 
Meeting in Houston Texas.  If you would like an electronic copy of the program 
please contact me via this e-mail.  All meeting and workshop information should 
be posted on the website shortly.
 
Regards,
TSH Convention Committee


Texas Society for Histotechnology, Inc.
33rdAnnual Symposium/Convention
   Histology: A Kaleidoscope of Change
 

Omni Houston Hotel
Westside
13210 Katy Freeway
Houston, Texas 77079
(281) 558-8338 
Room Rate: $125.00/night
Workshops $45.00 for 1/2 day


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[Histonet] RE: Reliable Bio repair/ refurbish contact in the Omaha area

[Nails, Felton]

Have you tried Tom Buck with Hawkeye biomedical 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, January 21, 2010 12:05 PM
To: histonet
Subject: [Histonet] Reliable Bio repair/ refurbish contact in the Omaha area

I am in need of a Reliable Bio repair/ refurbish contact in the Omaha area. I 
am less than satisfied with my current one, I have a 15 year history with them, 
but sadly I question their quality and integrity. The poor service is so 
blatant it is now become a reflection of me in my superior's eyes. I am in need 
of a refurbished cryostat and bio support for my equipment.

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

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[Histonet] RE: Paraffin Disposal

[Nails, Felton]

Allison we use tissue prep paraffin and it comes in cardboard containers, we 
save the empty containers to pour the waste paraffin in. Once it solidifies we 
dump it in the biohazardous container to be disposed of. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Monday, January 11, 2010 12:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin Disposal

Happy New Year to all.  How are you all disposing the paraffin that comes off 
of the tissue processor?  Do you consider it as biohazard waste and disposing 
it as such or are you putting it into the regular trash disposal?  I have been 
asked why I am disposing it the way I do.
I consider it as biohazard waste.  Your help in this will be greatly 
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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RE: [Histonet] Cutting Frozen Lung

[Nails, Felton]

You have to infuse the lung with a 50/50 oct sucrose solution prior to 
freezing. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker
Sent: Monday, January 04, 2010 9:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cutting Frozen Lung

Hello! 
 
I am looking for some helpful hints for cutting frozen lung tissue. I have 
tried just about everything I can think of!
 
Thanks!


  
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[Histonet] RE: FORMALDEHYDE

[Nails, Felton]

I have just purchased two Mopec MB600 grossing stations and we do not have this 
problem. Our pathologist actually rave about the unit. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham
Sent: Thursday, December 03, 2009 11:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FORMALDEHYDE

Good Morning Histo Land, I was just informed that on my last test sample for 
formaldehyde vapors was very high.  Right now I have to wear a respirator while 
grossing and assisting grossing.  We are looking at why the Mopec grossing 
station is not working in fact we have not had really good results since we put 
it in.  I'm wondering if anyone else is having the same problem or know of a 
better solution.  Our grossing station is the one that is self contained with 
filters. Also is anyone using with good result a formalin substitute? If so 
which one? 
Thanks and Have a great day,

Kathy Gorham, H.T.
Grande Ronde Hospital
La Grande Oregon


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