[Histonet] certified/uncertified

[Nancy Schmitt via Histonet]

Hello-
Looking for current practices with hiring:
if you were to hire a person with BS that has already been trained in histology 
but is not certified -
would they be hired at same rate as certified tech?
would they be expected to get certified?
It's a slippery slope...
Thanks
Nancy Schmitt HT, MLT(ASCP)


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[Histonet] prostate core/Decipher

[Nancy Schmitt via Histonet]

Hello!
I am looking for input on how you are treating prostate core biopsy specimens 
that go to Veracyte for Decipher Prostate testing.
We currently cut at 4 microns, 3 levels with 2 unstained at each level.
Veracyte requests the block - which would have very little left - or 10 
unstains at 5 microns.
Are you adjusting you protocol up front for all prostate cases?
Going down to 2 levels with additional unstains all at 5 microns?
I appreciate your thoughts,
Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor
Dubuque, IA


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[Histonet] Mohs

[Nancy Schmitt via Histonet]

Hello-
Is there any kind of grandfathering for HT certificate people cutting Mohs?
The surgeon does all inking and cutting.
Thank you
Nancy Schmitt, MLT(ASCP) CM HT CM



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[Histonet] moh's staining protocol

[Nancy Schmitt via Histonet]

Hello-
What is a best staining protocol for Moh's?
Is there one that starts in alcohol and ends in alcohol?  With no xylene or 
formalin?
Your thoughts are appreciated,

Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor


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[Histonet] claudin 18.2

[Nancy Schmitt via Histonet]

Hello-

Where are you sending Claudin 18.2 testing?

Thanks

Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor
MercyOne Dubuque


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[Histonet] baking after coverslipping

[Nancy Schmitt via Histonet]

Hello and Happy Friday!
Can you tell me about baking slides to dry the medium? I have not heard of this 
- we use a Leica CV5030 and then store the slides in order for several days to 
ensure they are dry before filing.  We are experiencing some "dry back"  (air 
under coverslip) issues and had someone recommend baking after coverslipping 
for 15 minutes and then they are ready to file immediately after being read.  
We are working with Leica technical on this issue, but this also came up in the 
process.   Package inserts do not lean to this.  I have been in the same lab 
for a long time and will appreciate your feedback!
Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor

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[Histonet] Sakura tissue processor timing pins?

[Staub, Nancy via Histonet]

Hello Histonet,

I am using an old, but good, tissue processor (Sakura Tissue Tek II)—the 
plastic timing pins (photo attached) are old and the plastic is starting to 
break.  I’ve tried to find replacement pins but have been striking out.   I’m 
hoping that someone might have one of these tissue processors sitting around in 
storage and might be willing to send me a few extra timing pins.   Or if anyone 
knows where I might be able to purchase some, I’d be happy to contact them 
(have checked ebay and a few tissue-processor re-furbishing businesses without 
luck).

Thank you very much for checking!
Best wishes,

Nancy Staub




Nancy L. Staub, Ph.D.
Professor of Biology
Gonzaga University
502 E Boone Ave
Spokane, WA 99258 USA

st...@gonzaga.edu
phone: 509 313 6636


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[Histonet] Mohs cryostat

[Nancy Schmitt via Histonet]

Hello and Happy Friday!
Any thoughts on Epredia vs Leica for cryostat?  This will be used for Mohs - I 
have only used Leica for frozen sectioning and appreciate any advice.
Thanks!
Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor


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[Histonet] BD Totalys - pap question

[Nancy Schmitt via Histonet]

Happy Friday!
For those running Surepath paps on the BD Totalys - have you seen any "haloing" 
effect? It is not the hats, and when the service group makes adjustments the 
"halo"  moves.  Slides are fine to be read, we just know that this should not 
be.  The service group has been great, I just though it was worth checking to 
see if anyone else had experienced this.
Thoughts appreciated,
Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor
MercyOne Dubuque, IA

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[Histonet] destain for IHC

[Nancy Schmitt via Histonet]

Hello-

Has anyone had success destaining an H and then running IHC on the same slide?

Thanks!

Nancy


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[Histonet] Moh's training

[Nancy Schmitt via Histonet]

Hello-
Looking for info. On Moh's training - did you go through the ASMH?  Trained by 
Dermatologist or pathologist?  Are you HT, HTL or uncertified?
I will appreciate any thoughts on this - a new opportunity for us; we currently 
do Frozen Section cutting, but not Moh's.
Thank You
Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor





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[Histonet] pathologist competency

[Nancy Schmitt via Histonet]

Hello-
Working with our Paths to update their competency policy - including the usual 
Gyn and Non gyn and surgical surveys along with their interdepartmental 
documentation; what are other folks including in this?
Thank much,
Nancy
Pathology Support Services Supervisor



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[Histonet] Cytology and Base mold release

[Nancy Schmitt via Histonet]

Happy Friday (unless you work the weekend),

  1.  Cytology - when cotest is ordered for Pap/HPV do you hold reading the pap 
until the HPV is completed?  Is there a recommendation on this?
  2.  Histology - when using base mold release, do you spray the molds once at 
the start of embedding, or in between every block embedded?
Your thoughts are appreciated,
Nancy Schmitt
Pathology Support Services


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[Histonet] HSV testing

[Nancy Schmitt via Histonet]

Hello-
What are you using for HSV IHC testing?
Thank you
Nancy

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[Histonet] ultrasafe formalin dispense

[Nancy Schmitt via Histonet]

Happy Friday!
Is anyone using the Ultrasafe Formalin Dispensing system? Here is a link
https://www.milestonemedsrl.com/us/product/ultrasafe/
Appreciate hearing from anyone on what your thoughts are - good or bad.
Thank you much,
Nancy Schmitt HT, MLT(ASCP)
Pathology Support Services
MercyOne Dubuque


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[Histonet] formalin in OR

[Nancy Schmitt via Histonet]

Hello-
I would appreciate input on how you are getting formalin on to the specimens:

  *   Is it done in OR
  *   Are specimens brough to pathology and add formalin there
 *   If so - does lab or OR add the formalin
  *   Other?
Are you Joint Commission?
Thank you!
Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services
Dubuque, IA

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[Histonet] GYN cytology reports

[Nancy Schmitt via Histonet]

Hello-
Looking to incorporate a comment in the bottom of the report regarding the 
screening process and wondering if anyone would be willing to share their 
format?
We are currently doing a yearly mailing to the phys.
Thanks
Nancy
MercyOne Dubuqe

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[Histonet] dissect aid

[Nancy Schmitt via Histonet]

Happy Monday!
For those using Dissect Aid (Statlab) - are you re-using this product for 
multiple specimens?
Can you please share what your process is for this?
Much appreciated,
Nancy Schmitt MLT, HT(ASCP)
MercyOne Dubuque

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[Histonet] solvent recycling waste

[Nancy Schmitt via Histonet]

What are folks doing with the waste from solvent recycling that contains traces 
of xylene?

Thanks
Nancy
Mercy, Dubuque



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[Histonet] release of body parts

[Nancy Schmitt via Histonet]

Hello-
We are seeing a bit more of patients that are requesting to take their body 
parts with them (uterus, POC, etc); I am talking home  - not the funeral home.
Are you using a release of body parts form to fill out with the patient?
Are you draining off the formalin, or sending in formalin with parafilm around 
the lid?
Thank you for your thoughts,
Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services
Dubuque, IA  52001


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[Histonet] cytology non-gyn screening

[Nancy Schmitt via Histonet]

Hello-
I know a lot of places have overlap with histology/cytology - do your cytotechs 
screen the non-gyn cases?
Do they regularly assist with FNA's?
How many paps are they expected to screen in a day?
This seems like a lot..
Input appreciated,
Nancy



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[Histonet] cytology billing

[Nancy Schmitt via Histonet]

Hello-
Looking for direction on billing practices  - specifically regarding non-gyn 
specimens that are screened  by a cytotech and then passed on to the 
pathologist for sign-out.
Can you bill twice for  that?  I am thinking not………thank you in advance
MercyOne Dubuque


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[Histonet] autopsy blocks

[Nancy Schmitt via Histonet]

Hello-

Can you tell me what you are doing with autopsy blocks?
How long are keeping these blocks/slides?
Appreciated,
Nancy
Dubuque, IA



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[Histonet] CBG recycling

[Nancy Schmitt via Histonet]

Hello-
We have been happily and successfully using the CBG recyclers for formalin and 
solvent recycling for many years.
In the last three months, we have been seeing failure with the Reagent #2 for 
the formalin testing-
it should be good for 3 months after opening, but we are seeing failure 
consistently after two weeks; wasting a LOT of reagent.
Company has been VERY difficult to in touch with and isn't being too 
helpful.
Anybody else having issue with this?
Thank You!

Nancy Schmitt HT, MLT(ASCP)
Pathology Support Services
MercyOne Dubuque




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[Histonet] AFB control and Recycling formalin

[Nancy Schmitt via Histonet]

Hello-

  1.  Does anyone have extra AFB control that we could obtain some from?


  1.  Recycling formalin:  if you are recycling formalin, do you recycle for 
any other sites?



 *   How do you charge for this?
 *   Do you charge for what is coming in  or what is going out?
Working through a multi-site dissolution and trying to future plan..
Thoughts appreciated!

Nancy Schmitt HT, MLT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories
250 Mercy Drive
Dubuque, IA  52001
Ph 563-589-9810

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[Histonet] frozen section/histo duties

[Nancy Schmitt via Histonet]

Hello and Happy Monday-
Would you please share what your processes are for:

  1.  HT's cutting frozen under direct supervision of pathologist - do you do 
this?
 *   Now HT has changed and requires Associates degree - but previous would 
be grandfathered in  - correct?
  2.  Non- certified staff working in histology with supervision - anything 
they DON"T do?
CAP is our guideline and I am reading - want to make sure I am not overthinking 
this but not missing anything.
Thoughts appreciated,
Nancy Schmitt HT, MLT(ASCP)
Pathology Support Services Manager

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Re: [Histonet] [EXTERNAL] Brain and spinal cord

[Stedman, Nancy via Histonet]

Hi – I use Excell Plus (alcohol based) for small brains (dog size and smaller). 
 I book them after 24 hours and then let them sit another 24 hours or more 
depending on the size, and changing the Excell.  It’s definitely not good as 
formalin, but my cases are veterinary necropsies and usually already have some 
autolysis already when they are submitted.  IHC seems to work just fine.  Have 
not tried ISH or nucleic acid extraction but the company that makes Excell Plus 
says it is suitable for these applications.  I have tried other alcohol based 
fixatives for small brains and none have been as good as Excell Plus.

-Nancy Stedman


-Original Message-
From: Yahoo via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, May 15, 2020 4:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Brain and spinal cord

Hi All! 
I’m looking for some suggestions please on fixation for brain tissue and spinal 
cord submissions from necropsies. We are currently using 10% NBF and ask our 
pathologists to leave the samples overnight (but that doesn’t always happen!!). 
Does anyone use alcohol-based fixatives? And if so, how long? Does it affect 
IHC or any other staining? Do you still process with other routine biopsies (14 
hour program)?
Thanks!

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[Histonet] clearing agents

[Lowen, Nancy via Histonet]

Good Morning,
Was wondering what preferences are as to CitriSolv versus Clearite 3 or Propar 
3?
Process lots of bone tissue and wondering which one everyone thinks works best.
Thanks
Nancy
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[Histonet] IHC Troubleshooting and Water Baths

[Nancy Schmitt via Histonet]

Hi Charles-

IHC staining - We also use the Leica Bond staining system and had tissue fall 
off in the beginning.  We blot the slides to remove any additional water and 
then place in drying oven for 30 minutes at 60 before placing on the stainer.

Water baths - at the end of each day the water is dumped, the bath is rinsed 
with bleach solution, rinsed with water and dried.

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 26, 2020 9:44 AM
To: Histo List
Subject: [Histonet] IHC troubleshooting

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Our pathologists are complaining that chunks of the dermis are missing from IHC 
slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


Message: 5
Date: Wed, 26 Feb 2020 17:09:14 -0500
From: Charles Riley 
To: Histo List 
Subject: [Histonet] Poll question
Message-ID:

Content-Type: text/plain; charset="UTF-8"

How often does everyone clean their water-bath?

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs



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[Histonet] erroneous results

[Nancy Schmitt via Histonet]

Hello-


Could you please share your process for erroneous results?  Scenario:  Specimen 
received, processed and reported out.  Physician office calls to say that the 
specimen was labeled with incorrect patient information.  1. Do you remove the 
results? 2. do you amend the report? 3. do you leave any trace?  4. is your 
process the same for surgical specimens as it is for GYN paps?

Looking forward to the discussion,

Nancy Schmitt

Pathology Support Services Mgr.

Dubuque, IA
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[Histonet] General Data Laser Track PH6

[Nancy Schmitt via Histonet]

Hello-
Is anyone using cassettes from a different manufacturer than the ones supplied 
with General Data?
We are looking for an alternative mesh cassette that works with the engraver 
since theirs does not utilize the whole inside of the cassette - there is an 
inner square space where the tissue is placed and a moat that surrounds it (for 
lack of a better explanation).
With appreciation,
Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager





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[Histonet] BOND Max from Leica

[Nancy Schmitt via Histonet]

Hello-
Looking for people that have installed a BOND Max from Leica in the past year, 
are using for general Anatomic Path IHC use, and are willing to have a chat and 
offer a few pointers.
Thanks,
Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories
Dubuque, IA  52001

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[Histonet] BOND question

[Nancy Schmitt via Histonet]

Hello-
Checking in to see if anyone else has seen any staining irregularities with a 
new BOND Max (OR III) or DS9800 detection kits.
I appreciate your thoughts,

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager


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[Histonet] waste removal

[Nancy Schmitt via Histonet]

Happy Friday!
Is your waste incinerated/autoclaved or some of both?
Is anyone having to marker over PHI on specimen containers before being removed?
Interested in processes and what is working best.
Thanks

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories

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[Histonet] Artisan AFB contamination

[Nancy Schmitt via Histonet]

Hello-

Has anyone had any issues with AFB contamination on the Artisan special 
stainer?  We are doing a monthly process with alcohol and drying of the 
containers and flushing the lines with alcohol per Technical support at Agilent 
- still seeing it pop up occasionally.

Appreciate any thoughts-

Nancy Schmitt

Untied Clinical Laboratories

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[Histonet] Surepath stains

[Nancy Schmitt via Histonet]

Hello-
This is regarding Surepath pap stain processing on the BD Totalys.
We switched to the new Totalys in late November and are having a lot of 
staining issues.
We have had many visits with field service.
Is anyone using this and having issues with inconsistent staining?
Thank you for your consideration,
Nancy
United Clinical Laboratories

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[Histonet] cytology listserv

[Nancy Schmitt via Histonet]

Hello-

I know many of us have overlap between histology/cytology/cytology prep.

Wondering if there is a helpful listserv available like this - or is my best 
chance to bring my questions here?

Thanks

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
Dubuque, IA  52001



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[Histonet] glass slides

[Nancy Schmitt via Histonet]

Hello-
We are seeing some warping with glass slides from Leica.

Looking for recommendation on what you are currently using?

Thank you

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories


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[Histonet] p16 and sperm morphology

[Nancy Schmitt via Histonet]

Hello-


1.   We are currently running P16 on the Leica Bond Max - are you running 
or reporting this any differently now that they are 510k FDA approved?


2.   Sperm Morphology- are your cytotechnologists reading and reporting out 
sperm morphology or are your clinical lab techs?


Responses appreciated:)

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001



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[Histonet] Downtime

[Nancy Schmitt via Histonet]

Hello All-
We recently experienced some downtime with our computer systems - more than the 
usual 1-2 hours for maintenance.  Some was isolated to our Anatomic Pathology 
system, and some of the downtime was organization wide.  We are an independent 
laboratory system that operates with Cerner CoPath for Anatomic Pathology, MLab 
from McKesson for LIS and we then interface with Powerchart and EPIC at our 
hospitals.  There is nothing like a little downtime to make you take another 
look at your processes.  My questions:

1.   What computer product are you currently using for Anatomic Pathology?

2.   Are you satisfied?

3.   Do you have your own IT group or is it maintained by outside resources?

4.   Are you interfaced with hospital or other?

5.   What critical functions do you provide during downtime?  Are you 
giving verbal reports?  Are you typing reports on a backup laptop?
I appreciate any input you are willing to share and I am sure there will be 
others taking note of this valuable conversation!

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager



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[Histonet] Pineapple sponge sections

[Thomas, Nancy via Histonet]

I would like to hear from anyone with experience in working with the pineapple 
sponge and other similar types.  A future project will include processing, 
sectioning and staining of the samples in order to identify them by viewing the 
arrangement of the spicules.  I am wondering if cryo, paraffin or plastic would 
be the best method?  How much fixation is necessary?  If anyone has working 
protocols they would share, I would be so thankful.


Nancy Thomas

Senior Lab Manager, Histology Core

Stowers Institute for Medical Research

1000 E. 50th Street

Kansas City, MO  64110

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[Histonet] "Budget" single slide scanners

[Stedman, Nancy via Histonet]

Hi Histonetters -

Does anyone have experience with the low cost single slide scanners like the 
uSCOPE machines?  Are they any good?   Am looking for a slide scanner but have 
a limited budget ...   Thank you!

-Nancy Stedman


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[Histonet] cassette labeling

[Nancy Schmitt via Histonet]

Hello-
Is anyone out there using the PH1 or PH6 cassette labelers from General Data?   
The technology is different from the original and we are looking to upgrade.  I 
shall appreciate any information/comments you are willing to share.
Thank You,

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
Dubuque, IA  52001



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[Histonet] Is anyone running IHC for pyrimidine dimers in fixed tissue

[Stedman, Nancy via Histonet]

Looking for some help with running IHC for pyrimidine dimers in fixed tissue?  
Is anyone doing this, have any recommendations - preferred antibodies, etc?  
Thank you -

-Nancy Stedman

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[Histonet] validation

[Nancy Schmitt via Histonet]

Good Day!
When performing validation on IHC, we use 20 positives and 20 negative cases.

When testing for negative, can the normal tissue surrounding the tumor be 
counted as negative?

Does the negative tissue need to be tumor that is negative for the AB or just 
normal tissue?

Thank you for your consideration,

Nancy
Dubuque, IA
Ph. 563-589-9810



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[Histonet] ANP.11640 competency for Non-Pathologist

[Nancy Schmitt via Histonet]

Hello-
Would you share what you are doing to show competency for Non-Pathology (HT 
with high complex) staff that are grossing derms, GI's, etc.?

Thank you

Nancy


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[Histonet] Histocryl acrylic system

[Thomas, Nancy via Histonet]

I have been trying different resin kits now that Immuno-bed is no longer 
available.  I am ready to try 'Histocryl' but the instructions are unclear to 
me.  Has anyone had experience with this brand?  If so, I would like to ask a 
few questions.
Thank you in advance,

Nancy Thomas
Senior Lab Manager, Histology Core
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO  64110

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[Histonet] PPE and countertop cleaning

[Nancy Schmitt via Histonet]

Good Morning!
We are circling again on these two hot topic items!
Please share what you are using for PPE for histology processes and do you also 
wear for embedding/cutting?
Countertop cleaning?  We went to a purchases wipe and it is eating away the 
countertops:(  Would you please share what you are using and what your process 
is for countertop cleaning?
We are CAP inspected.

Thank you much for your input

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager




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[Histonet] pre cut control slides

[Nancy Schmitt via Histonet]

Hello-
How are you handling precut control slides for IHC stains?  How long do you 
keep them before throwing?
I found some info (on histonet) on special stains but not on IHC stains.
Thanks
Nancy Schmitt HT, MLT(ASCP)
United Clinical Laboratories


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[Histonet] validation for special stains

[Nancy Schmitt via Histonet]

Good Morning-

What is everyone doing for validation on special stains?  We have a new Artisan 
but I am struggling with finding exact validation specifications.  It will be 
difficult to find 10-20 cases on some of these stains.

Thoughts and Thank you!

Nancy
Pathology Support Services Manager
United Clinical Laboratories

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[Histonet] egg albumin

[Nancy Schmitt via Histonet]

Good Morning-

Thoughts on shelf life of egg albumin?

Thank you

Nancy Schmitt MLT, HT(ASCP)


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Re: [Histonet] [EXTERNAL] Re: Tissue fixation

[Stedman, Nancy via Histonet]

I would bet there are quite a few pathologists on this mailing list (like me) 
who are here because we respect histo techs and know you have valuable 
information to offer!

I agree, cautery artifact is pretty easy to tell from poor fixation, so it 
should be easy to determine if that is the problem.  Sometimes, specimens that 
are submitted smashed in a cassette may have poor fixation of the tissue in 
contact with the cassette.  Or if they are submitted sponged and floating, some 
tissue may not be fully immersed in formalin.  Also, crush artifact may 
resemble poor fixation, so maybe the clinical team is not handling the tissue 
gently enough?  

I read veterinary specimens; we don't do LEEPs so I don't know if any of these 
scenarios are possible, but they are things I see frequently.  

-Nancy Stedman


-Original Message-
From: Mayer,Toysha N via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 2:15 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] Tissue fixation

I agree with Rene.  To discredit the pathologist theory  show if all of the 
specimens from the run are not fixed properly.  Then show if it is just the 
LEEPs.  Then show if it is that particular clients specimens?  Then onto that 
client's LEEPs.
That should prove your problem lies with the client handling and not the 
fixation on your end.  Cauterization can cause burning of the specimen, but not 
look unfixed. If it was left out of formalin, autolysis can set in.
The situation shows the god-complex some physicians have and the lack of 
respect they have for their techs.  
This is why we should all be certified and keep up with continuing education, 
so that these pathologists will respect us.
Ok, I'm going to get off my soapbox now, before I say something ugly.

Toysha Mayer






--

Message: 3
Date: Fri, 31 Mar 2017 12:50:37 +
From: T H <thiggin...@msn.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Tissue Fixation
Message-ID:

<sn1pr19mb060735d7b896c67b0223e098d8...@sn1pr19mb0607.namprd19.prod.outlook.com>

Content-Type: text/plain; charset="iso-8859-1"

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim



--

Message: 4
Date: Fri, 31 Mar 2017 13:32:35 + (UTC)
From: Rene J Buesa <rjbu...@yahoo.com>
To: T H <thiggin...@msn.com>,   "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Tissue Fixation
Message-ID: <1730848134.8065268.1490967155...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?Ren? 

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
<histonet@lists.utsouthwestern.edu> wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.? She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).? She claims we must be diluting our formalin to cause this 
issue or "somethin

[Histonet] Non-gyn cytology

[Nancy Schmitt via Histonet]

Good Morning-

Can you share with me how you are handling non-gyn cytology specimens?  Are you 
working with them under a hood?  In a TB room?
At a counter in the histology lab that has good negative pressure?  Fixed vs. 
unfixed?

Appreciate your input-

Nancy
United Clinical Laboratories


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[Histonet] ConfirmMDx

[Nancy Schmitt via Histonet]

Hi All-
Looking for feedback on experience with prostate specimens going to ConfirmMDx 
for testing:
Reimbursement, physician satisfaction, blocks and slides or whatever else you 
have experienced.
Thank you,
Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories



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[Histonet] Decal following ISH?

[Thomas, Nancy via Histonet]

Hello all,
I received whole mount samples in 70% ethanol for paraffin processing.  The 
samples are snail embryos and the researcher already did in-situ on them.   
Because they are of varying ages, some of the shells will section without 
decal, but some will need it.  However, this step was not done before the ISH 
staining.  Does anyone know if decalcification can follow an ISH procedure?  I 
have searched a few protocols online, and each of them did the decal before 
hybridization.  I have not yet found one where the decal followed the 
procedure.   My plan is to start with one embryo and try it to see what 
happens.  Maybe the signal will remain, or maybe not.  Has anyone done it this 
way and know the answer before I try?
Thank you,

Nancy Thomas
Senior Lab Manager, Histology Core
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO  64110

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[Histonet] cryostat decontamination

[Nancy Schmitt via Histonet]

ANP.23410 states that cryostat is defrosted and wiped down at regular 
intervals.  What if you have UV decontam?  Is there a ruling on that?

Thanks

Nancy Schmitt MLT, HT(ASCP)
Dubuque, IA

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[Histonet] HT grossing

[Nancy Schmitt via Histonet]

Hi All-
I have a couple things I am looking for assist on today:

1.   Are your HT's doing frozen section cutting?  Are they also doing the 
frozen section gross and inking?  Are they staining the slides?  We are 
interested in doing this and looking for how others do.

2.   Do your HT's gross smalls?  What does that include? How did you train 
them and can you share that information?  Is there a guide out there you would 
recommend?
Appreciate your input,
Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
Dubuque, IA

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[Histonet] Pathology physician assistant

[Nancy Schmitt via Histonet]

Hello-
Our facility has 6 pathologist and one PA; I am wondering if anyone has a job 
description they would be able to share.
We are doing some restructuring and wondering how the PA fits in at other 
places and what tasks they are performing besides grossing specimens; this is 
the only place this PA has worked.
I appreciate any input,
Nancy Schmitt HT, MLT(ASCP)
Pathology Support Services Manager
Dubuque, IA

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[Histonet] floor vibration

[Nancy Schmitt via Histonet]

Happy Monday!
We are moving to a new space and part of our area is above the laundry - there 
is some vibration from there.  Does anyone have any experience with this and 
could you please share how you accommodated this?  Special flooring, pads, etc.
Thank you much!

Nancy Schmitt HT, MLT (ASCP)

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[Histonet] congo red - decolor

[Nancy Schmitt via Histonet]

Happy St. Pat's Day!

Any thoughts on how to decolorize Congo Red stains that were done on fat pad 
slides?
We appreciate any insight you might offer:)

Nancy
United Clinical Labs
Dubuque, IA

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[Histonet] paraffin block storage

[Nancy Schmitt via Histonet]

Good Morning-
I do not find a CAP guideline for monitoring room temperature for block storage.
Thoughts?
Thank you
Nancy

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[Histonet] embalmed cadaver tissue

[Nancy Schmitt via Histonet]

Good Afternoon!
Does anyone work with embalmed cadaver tissue?  How is this handled for 
successful processing?
Any advice appreciated.
Nancy

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[Histonet] toluidine blue

[Nancy Schmitt via Histonet]

Hi All-
Is anyone using Toluidine Blue on air dried touch prep slides?  Specifically 
for endobronchial ultra sound specimens (ebus).  I am wondering if you can tell 
me what you are using for a toluidine blue recipe.
Thank you for your help-

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories
Dubuque, IA  52001
Ph. 563-690-4142

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[Histonet] Cryostate decon ANP.23410

[Nancy Schmitt via Histonet]

Good Morning-
Defrost and decontaminate with TB disinfectant weekly if used daily.  How are 
you best managing this and what are you using to decontaminate for TB?
Thank you

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001





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Re: [Histonet] Hematoxylin Precipitate

[nancy lowen via Histonet]

Yes, I have had the same experience.Filtering  it seems to help. 


 On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet 
 wrote:
   

 Hello all,

                Has anyone using Richard Allen Hematoxylin-2 noticed an odd 
artifact on the slides after using the Hematoxylin for more than a few days on 
their stainer? We are seeing small spore or pollen-like blue dots here and 
there on the slides. It is not coming from the water bath or our water supply 
on the stainer. I used sterile gloves, opened a new case of slides, dipped them 
in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, 
air-dried and coverslipped them, and the blue dots were there. The only way we 
got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, 
which is a bit expensive.

                Thanks for your input, and if you can recommend a different, 
reasonably priced hematoxylin, that would be awesome.

Cheers,

Sandy

 

Sandra J. Cheasty, HT (ASCP)

Histology & Necropsy Supervisor

UW-Madison, School of Veterinary Medicine

 

 

 

 



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[Histonet] H. Pylori

[Nancy Schmitt via Histonet]

Good Morning-

I am looking for H. Pylori control - does anyone have any extra lying about?  
What are you currently using?  Purchasing?
I appreciate any and all feedback!

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001
Check us out at www.uclaccess.com<http://www.uclaccess.com/>





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[Histonet] PAP stains done by hand

[Nancy Schmitt via Histonet]

Happy Friday-

Could you please share how you are handling the potential for cross 
contamination in non-gyn pap specimens?  Are you filtering/changing out 
solutions between each case?

I appreciate your input-

Nancy
Histology Coordinator
Dubuque, IA  52001
Check us out at www.uclaccess.com<http://www.uclaccess.com/>





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Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories

[Stedman, Nancy]

We are using Excell Plus made by American Master Tech.  It is a combination of 
alcohol, ethylene glycol, and glyoxal.  It does not fix quite as fast as 
formalin but fixes just as well as long as tissue samples are small and not too 
fatty.  We work in veterinary pathology and mostly with necropsy tissues, and 
try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. 
 It definitely won't fix an entire intact brain no matter how long you let it 
sit so we book the brains into thin slices 24 hours after collection.   We have 
done some limited IHC and DNA extraction from Excell Plus fixed tissue and have 
not had any issues.   We tried another low tox fixative too - Statlab's GTF 
formalin substitute, which is glyoxal - and it did not fix as well as Excell 
Plus.  GTF fixed CNS tissues especially had a lot of artifact like shrunken 
neurons.   

Hope this is helpful.  I would also be curious to know how many labs use 
formalin substitutes because it seems like they have not caught on much (or at 
all) in the veterinary world.   

-Nancy 


Nancy L. Stedman DVM PhD Dipl ACVP
Veterinary Pathologist
Busch Gardens Tampa
nancy.sted...@buschgardens.com



-Original Message-
From: Adesupo, Adesuyi (Banjo) [mailto:abades...@nrh-ok.com] 
Sent: Monday, July 13, 2015 12:52 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can 
Cause Cancer in a Finding That Has Implications for Anatomic Pathology and 
Histology Laboratories

 Hi,
   I read this article (National Academy of Sciences Confirms That 
Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic 
Pathology and Histology Laboratories) this morning.
I wanted to know whether some of you guys out there are using Formaldehyde 
substitute.


Best regards,

   Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
   Histology Supervisor
   Norman Regional Health System,
   Norman, OK 73071.
   Tel: 405- 307- 1145
   abades...@nrh-ok.commailto:abades...@nrh-ok.com

==
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[Histonet] fat pad smears for amyloid

[Nancy Schmitt]

Good Morning!

How are you pretreating fat pad smears for amyloid?  Fixing in 95% and then air 
drying before staining?  We are running them on an Artisan stainer.

Thanks for your input-
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA




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Re: [Histonet] Cryojane tape-transfer and hydration of sections

[Thomas, Nancy]

Ana,
We once tried 4x coated slides thinking, like you, that it would deliver a 
better quality section and would stay on the slide better.  Our researchers did 
not like them at all because of the high level of background  staining and 
fluorescence.  We only use 1x now.

Nancy

-Original Message-
From: Ana Maluenda [mailto:amalue...@svi.edu.au] 
Sent: Tuesday, June 09, 2015 7:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryojane tape-transfer and hydration of sections

Hello Histonetters! 

I was wondering if there is anyone out there who is currently using the 
CryoJane Tape-transfer system for bone sections. I have been trying to get good 
sections for mice tibia and femur and often find myself with problems. 

I spent a good time trying to improve the quality of sections (tried different 
temperatures, thicknesses and speed as well as 1x vs 4x coated slides). The 
sections improved (although not perfect), but I was planning to move on for a 
staining trial. Currently, the sections are taken at 5um, in a 4x coated slide, 
at -26oC. However, now I get myself with a problem when hydrating the sections. 
At the moment, once the sections are taken, they are kept in -20oC (for 
short-storage). They are then taken out and left at RT for 30 min before 
hydration with PBS for another 30 min. I have noticed that it seems as if the 
OCT around the sections doesn't completely dissolve. I have already tried PBS 
vs dH2O and played around with times (from 5 min to overnight) with no 
differences. 

Has anyone had this happening before? Can it be because of the 4x coat? Is 
there anything I can do to? And would this be a problem for immunofluorescence? 

Any advice would be much appreciated! 

Thanks in advance, 

Ana 


Ana Maluenda 
Research Assistant 
Stem Cell Regulation Unit 



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RE: [Histonet] Re: H. Pylori Testing

[Stedman, Nancy]

I believe IHC is more sensitive than the special stains too.  One caveat for 
anyone who works with veterinary samples - the H. pylori antibodies are 
specific for H. pylori, so I have not found these antibodies to be helpful for 
evaluating other species with helicobacter-associated gastritis.  One exception 
is the antibody made by Biocare which seems to stain some of the feline 
helicobacters, and maybe others too (have not tried).

-Nancy Stedman

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Tuesday, April 28, 2015 12:33 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: H. Pylori Testing

From the pathologist's viewpoint, immunohistochemical stains for
Helicobacter are much faster to read than are the old dye methods such as 
Giemsa.

Ventana claims that you can break even with one of their stainers if you 
produce 600 billable slides a year (unless they've changed that number - a rep 
can tell you.

It's likely that we are going to get a lot more resistance in the future from 
the third parties about paying for a Helicobacter stain done in advance of the 
pathologist's looking at the slide. I'd think about that before investing very 
much in anything.

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] interface between Bond and Cerner CoPath Plus

[Nancy Schmitt]

Does anyone currently have an interface between the Leica Bond stainer and 
Cerner CoPath plus?  How does it work? Cost?
Thank you
Nancy Schmitt MLT, HT(ASCP)



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[Histonet] waterbath/faucet disinfection

[Nancy Schmitt]

I am curious to know what others are doing (if anything) to disinfect their 
faucets/waterbaths on a daily basis.
Our procedure uses bleach on both daily - that will now change as the bleach 
will ruin the finish on our newer waterbaths.
Thanks for your input-
Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator




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RE: [Histonet] BS in Histotechnology

[Stedman, Nancy]

As a pathologist I'd like to apologize for all the pathologists who have made 
comments like this.. forget trained monkeys and dogs, most (all?) pathologists 
cannot cut slides either, at least not slides they'd want to try to read.   I 
know I can't.   

-Nancy Stedman 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner
Sent: Tuesday, March 24, 2015 4:26 PM
To: Paula Sicurello; Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; 
Marcum, Pamela A
Subject: RE: [Histonet] BS in Histotechnology

I once worked with a Pathologist who said she was in a group meeting of other 
pathologists when one of them blurted out that a trained monkey could cut 
slides.  My pathologist, having had the opportunity to review some cases from 
the offender's laboratory, promptly replied Yes, and with the quality of your 
slides it looks like you did just that.  She shut down the other pathologist 
really quickly, and as far as I know, we never received another case to review 
from him.  My pathologist was not about to let that kind of arrogance stand.  
She was one of the best bosses I ever had!

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, March 24, 2015 3:47 PM
To: Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; 
Timothy Morken
Subject: Re: [Histonet] BS in Histotechnology

I've had more than one pathologist tell me a monkey could do my job.
Though one of them said it with a smile and added a very highly skilled and 
well trained monkey, he was one of the few who knew better.

How many of us monkeys have trained the whining and complaining residents how 
to do things correctly?

Paula

On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones mjo...@metropath.com
wrote:

 OMG Pam~ our pathologist said the exact same thing to us when we 
 started our Grossing training.
 Sheesh. . .
 Michael Ann




 On 3/24/15, 11:52 AM, Marcum, Pamela A pamar...@uams.edu wrote:

 That was nicer than the pathologist who told me years ago, any 
 monkey could be trained to do my job.  I basically did not take the 
 job I was interviewing for at the time.  At least the next interview 
 went a lot better and I did take the job.
 
 Pam
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 Sanders, Jeanine (CDC/OID/NCEZID)
 Sent: Tuesday, March 24, 2015 12:30 PM
 To: Sue; Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: RE: [Histonet] BS in Histotechnology
 
 I agree, BUTas long as many pathologists think you can 
 teach any trained dog how to section histology will never have the 
 recognition those of us that have studied and trained deserve.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
 Sent: Tuesday, March 24, 2015 12:59 PM
 To: Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: Re: [Histonet] BS in Histotechnology
 
 This is a fight that we continue to have with hospital administration.
 In my opinion histologists are just as important and needed as MT.  
 Even though there is an increase in automation in pathology the hands 
 on of a histologists is most important.  The fact that hospital still 
 consider a lower entry job is the reason there are not more of us.
 It is quite frustrating.
 
 Sue
 TJUH
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[Histonet] preparing a 20% polyvinyl alcohol solution

[Thomas, Nancy]

Dear Histonet,
I am making a 20% PVA solution for cryo embedding murine bones, following John 
Tarpley's recipe found in his JOH article, March 2003.  He mentions no problems 
in making it, but after 3 days on the stir plate, it still has not gone into 
solution.  The PVA settles very quickly, even before I can pour it into a mold 
to freeze and test.  Is there a trick to this that I need to know?  Any advice 
would be so great.
Thank you,
Nancy Thomas

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Re: [Histonet] RE: Masson Trichrome stain

[nancy lowen]

Just out of curiosity--what strength of Picric acid is in your Bouins 
fixative?Nancy 


 On Thursday, March 12, 2015 2:02 PM, Gayle Callis 
gayle.cal...@bresnan.net wrote:
   

 I have been following the string of inquiries about using metal forceps with
Masson's Trichrome staining.  I was taught many years ago to avoid metal
forceps or the older metal tissue cassettes with Bouins.  I am scrambling
to find the actual reference.  The reason given was acids in Bouins corrode
metal.  This may be a lost bit of information since the overall majority of
labs now use plastic tissue cassettes.  Case in point:  using acidic
descaling solutions for household cleaning i.e. showers/tubs or coffee
machines.  These solutions come with warning to avoid metal fixtures and
stainless steel sinks.  Accidental contact of acids in a stainless sink
causes the metal  discolor, indicating corrosion - been there, done that to
a stainless steel sink.  I so use metal forceps to move slides between Mass
Tri staining solutions (and silver staining solutions) without problems per
John Kiernan's comment.          

 

Not using metal forceps with silver stains i.e. GMS, reticulin, is to avoid
metal ion contamination which is more likely due to with poorly washed
glassware.  In the past, we dipped metal forceps in melted paraffin, very
messy since paraffin comes off on slides and in hot staining solutions.
Disposable plastic forceps are cheap but break easily resulting in a dropped
slide.  Teflon forceps are pricey but it was a challenge to hold slides.
Hopefully there are teflon forceps that work better than the one we used?
We tried a teflon tipped metal forceps but not worth the price as teflon
wears off the tips to rexpose metal.    Weigerts hematoxylin  is not
affected by metal forceps since there are no acid components to corrode the
metal although Weigerts can stain the forceps.  Simply wash the forceps
in dilute chlorine bleach then soap and water.  I agree with John Kiernan
and now use metal forceps to move slides between staining solutions in both
Massons trichrome (and silver methods)  without problems.  If people want to
use plastic or teflon forceps, I understand the reasons.      

 

As for not rinsing before going into Aniline Blue (or light green) in
Massons trichrome, there is a reason for this.    Sheehan and Hrapchak state
verbatim  The phosphomolybdic acid and phosphotungstic acid thus acts as a
link connecting basic groups of the connective tissue fiber to the basic
groups of the dye i.e. aniline blue.  The PM/PT acid treatment has the
ultimate effect of making an amphoteric dye that would ordinarily act as an
acid dye to change and act as a basic dye.  These authors also say
Although the exact mechanism of how the stain works is unknown, some
theories are available.    By rinsing away the PT/PM, the link may be
weaker hence one goes from PT/PM directly into aniline blue (sometimes light
green or fast green).    Bierbrich Scarlet/acid fuchsin and aniline blue
(light green or fast green) solutions can be filtered back and reused many
times.  PT/PM and 1%  acetic acid solutions  should be discarded after use.


 

Instead of kits due to expense and some kit deviations from classic Massons
Trichrome method, I found I could buy excellent, reliable single staining
solutions i.e. Biebrich Scarlet/Acid Fuchsin and Aniline Blue from Newcomer
Supply or Poly Scientific to avoid exposure when weighing out carcinogenic
dyes.  Bouins is purchased from the vendor with the best price.  However,
PT/PM and acetic acid single use solutions were still made in house to save
costs.      

 

I strongly recommend reading John Kiernan's   Methods for Connective
Tissues  from his book , Histological and Histochemical Methods Theory and
Practice  for better explanation and understanding of Massons Trichrome
chemistry.    Collagen and muscle staining methods in Sheehan and Hrapchaks
Theory and Practice of Histotechnology is not recent but a good start.


 

Whew, a long reply but hope helps...

 

Gayle Callis

HTL/HT/MT(ASCP)  

 


 

Written is:  

 

Justine,

 

I do not have any metal forceps in the special stains area, due to the
reaction that they can cause when staining with silver.  As a rule of thumb,
it is just easier to use plastic all the way around.  

The Carson text does not state the use of only plastic forceps, but I would
think that maybe they are concerned with a reaction between the Weigert's
and the metal.  That would be a stretch.

As for no water before aniline blue, I believe the concentration is very
weak and the water may dilute they dye even further.  This would affect the
staining results.

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)

Instructor/Education Coordinator

Program in Histotechnology

School of Health Professions

UT M.D. Anderson Cancer Center

713.563-3481

--

 

Message: 4

Date: Tue, 10 Mar 2015 00:31:56 -0500

From: John Kiernan

[Histonet] Diff Quik troubleshooting

[Nancy Schmitt]

Good Morning-
Random FNA case where the slides turn blue as they dry.  We have tried taking 
them back through stains, destaining and restaining, etc.  FNA smears are 
air-dried when we receive them on plain slides, unfixed.
Any thoughts appreciated.

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA



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RE: [Histonet] Controls:

[Stedman, Nancy]

Do you mean using Slim Jims right out of the package, as is, for Gram controls? 
 What is in those things - sounds like a food safety issue!


-Nancy Stedman

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Thursday, March 05, 2015 1:01 PM
To: 'Jb'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:

Years ago (70's and 80's) Slim Jims were used as Gram pos  neg controls in all 
the labs at AFIP.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:

Off the wall question, I have been told that slim jims (pepperoni stick) at the 
gas station can be processed and used as good gram controls. Has anyone done 
this and do they work for GMS also?

Thank you,

Sent from my iPhone
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[Histonet] CBG recyler and recycled Formalin

[Nancy Schmitt]

Roy-
We use CBG recyclers - one for alcohol/xylene and one for formalin.  The 
formalin is tested and with a couple simple equations you determine whether you 
need to add water or formalin concentrate to get back to 10%, figure out total 
amount of formalin and another equation to determine the amount of buffer to 
add.
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA

Date: Fri, 27 Feb 2015 14:41:51 -0500
From: Roy, Ryan ryan@va.gov
Subject: [Histonet] CBG recyler and recycled Formalin
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:

15f883394eab744e99e1c7e1b98730490178bf4ec...@r04bynmsgb1.r04.med.va.gov

Content-Type: text/plain; charset=us-ascii

Hello,

We are getting a new CBG that recycles xylene , alcohol, and formalin.

We purchase buffered formalin? Does anyone know if after recycling the recycled 
formalin would or would not need be re-buffered??

Thanks in advance,


Ryan Roy HTL (ASCP)
Histology Lab
Manchester VA
718 Symth Rd
Manchester NH 03104
(603) 624-4366 ex 6640



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[Histonet] Prostate Biopsies

[Nancy Schmitt]

We do not have a process that occurs at the urology office.
Our PA places a colored marker made from Histogel and marking ink into the 
cassettes and includes that information in the gross dictation.

Nancy Schmitt MLT, HT(ASCP)
__

From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Tuesday, February 24, 2015 5:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prostate Biopsies

Short of using a bar-coded tracking system, does anyone use  color-coded 
formalin containers or cassettes for prostate biopsies (for the urology office 
staff) to confirm the patient's identity?  Thanks.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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[Histonet] Looking for ribbons for old Surgipath VCP cassette printer

[Stedman, Nancy]

Hi everyone -

For those of us who are still in the dark ages... have an old Surgipath VCP 
cassette printer; does anyone know where to get replacement ribbons for these 
machines these days?   Does anyone know who supports these machines anymore?  
Thanks so much -

-Nancy Stedman



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RE: [Histonet] Troponin is biomarker of heart attacks

[Stedman, Nancy]

Hi Jorge - If I understand your question correctly - it is because cardiac 
troponin is specific for cardiac muscle damage.  Other muscle enzymes/proteins 
we can assay for could also be elevated with skeletal muscle damage.  

-Nancy Stedman

  

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Jorge A. Santiago-Blay 
[blayjo...@gmail.com]
Sent: Thursday, February 05, 2015 4:01 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Troponin is biomarker of heart attacks

Dear Histonnetters:

I am not sure the range of expertise of the members may reach this far but
let me try.

Question: Could sometone tell me (blayjo...@gmail.com) why is
blood troponin is biomarker of heart attacks. I am aware that is is not the
only biomarker but nowhere have I yet seen an explanation why not
tropomyosin of other protein components of the cardiac muscle that may find
themselves in the blood stream as a result of damage or death due to the
heart attack.

If you know of a human physiology / human anatomy listserver, please, let
me know.

Gratefully,

Jorge
blayjo...@gmail.com


--
Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
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[Histonet] acid fast control tissue

[Nancy Schmitt]

Happy New Year!

My current source for acid fast control tissue is no longer available.  I would 
appreciate any direction...

Thank you-

Nancy

Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA  52001
ph. 563-690-4142




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[Histonet] Prostate Needle Bx

[Nancy Schmitt]

Our group puts the biopsies between sponges - they don't move around or get 
tangled and we process them with the small biopsies.  Good results.

Nancy Schmitt
United Clinical laboratories
Dubuque, IA
  

  Sent: Wednesday, December 03, 2014 11:49 AM
  To: Histonet Post (histonet@lists.utsouthwestern.edu)
  Subject: [Histonet] Prostate Needle Bx
  
  How do others process prostate needle bx's - what kind of cassettes 
 do you use?Do you use sponges?Do you
wrap the tissue in lens paper?Do you process them on a biopsy (short) 
run?Our bx's always seem to be less than optimal, but we do not have 
problems with other small bx's.
  
  Laurie Colbert



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[Histonet] RE: MMA Mounting Issue with Haupts Adhesive

[Thomas, Nancy]

Jesse,
I have discovered that there are many different ways to work with MMA.  
Probably no two techs are doing it exactly the same because there are so many 
steps in which to optimize it for your lab.  From reading your protocol, I see 
similarities and differences.  Rather than saran wrap, I use a thicker plastic 
that I cut into strips ( the size of the slide) from the plastic bags that we 
use for discarding paraffin waste.  Although this is different from your 
protocol, I still think that what I do to remove the plastic might work for 
you:  just before removing the plastic strip, I shoot it with a blast of freeze 
spray.  The plastic just about falls off.  

Nancy Thomas
Stowers Institute for Medical Research
Kansas City, MO

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hernandez, 
Jesus Willibaldo
Sent: Wednesday, December 03, 2014 11:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MMA Mounting Issue with Haupts Adhesive

Hi all,

I had a question about how to properly mount MMA sectioned slides using Haupt's 
(Dorn and Hart Microedge). My protocol is as follows:


1.   Stick Glass Slide into 50mL Falcon Tube with Haupts solution (1:1 50% 
EtOH:50% Haupts).

2.   Rotate the tube to ensure entire coating of glass slide.

3.   Let glass slide dry in fume hood for about 10minutes.

4.   Remove cut microtome sections (range: 50-5microns).

5.   Place cut sections in 70%EtOH to soften the plastic and unroll. 
(sections are coming out rolled up like scrolls)

6.   Remove a section from the 70%EtOH using forceps and try unrolling it 
with a needle on a glass slide.

7.   Once the section(s) is stretched on the coated glass slide, cover with 
saran wrap.

8.   Cover the wrapped slide with coarse filter paper and use a small seam 
roller to roll the sections on the glass slide as flat as possible. This will 
also help remove excess 70%EtOH.

9.   Remove the filter paper and stack glass slides in a slide press or 
similar device. Place tongue depressors or small pieces of wood equivalent to 
the slide length that are about 1cm thick at the end of the glass slide stack.

10.   Let slides dry over night at room temperature in press.

11.   Remove the slides from the press and unwrap the saran wrap to expose your 
adhered sample.

Questions/Comments:


1.   When I pull off the saran wrap, the sample sometimes comes off. The 
plastic in the sample turns white and dries out and does not attach. This is 
seen in the majority of my tissue sections.

2.   The only time I have noticed consistent attachment is for a 5micron 
sized section.

3.   How can I adjust the microtome so that the samples do not come off so 
scroll-like? My cutting angle is typically either 0 or 2.5 degrees. I also 
apply 70% EtOH to the block surface to soften the plastic.

Overall goal:
Get the tissue sections adhered to the glass slides so that I can proceed with 
staining. Thank you all.

Regards,

Jesse Hernandez


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[Histonet] Slide and cassette printers

[Nancy Schmitt]

We are using cassette and slide labeling system from General Data - like it a 
lot!



Nancy Schmitt MLT, HT(ASCP)

United Clinical Laboratories

Dubuque, IA



Message: 3

Date: Tue, 16 Sep 2014 17:04:45 -0400 (EDT)

From: Kathleen Roberts 
kgrob...@rci.rutgers.edumailto:kgrob...@rci.rutgers.edu

Subject: [Histonet] Slide and cassette printers?

To: histonet 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

Message-ID:

  
fc88267d9429736ab61eb21bbf01bccf.squir...@webmail.rci.rutgers.edumailto:fc88267d9429736ab61eb21bbf01bccf.squir...@webmail.rci.rutgers.edu

Content-Type: text/plain;charset=iso-8859-1



What are all of you using these days?  What would be good for a small histo lab 
that has the potential to grow?  (I am already aware of what Leica and Thermo 
have from the archives here, but I am still interested in your opinions, of 
course.)  Has anyone tried the printers, labels and attachment machine from 
Brady?



Thank you all so much,

Kathleen







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[Histonet] how long to keep IHC validation materials

[Nancy Schmitt]

We are having a discussion on how long to keep the slides for IHC validation?  
As long as you have the instrument? Or can the slides be disposed of at some 
given time (5years?)?
Thank you for your input-

Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator
United Clinical Laboratories
visit us at www.uclaccess.com




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Re: [Histonet] Water/Bubbles

[nancy lowen]

I have also had this problem recently.
Nancy Lowen 


On Thursday, August 7, 2014 1:23 PM, Julie Bowman tbilaborat...@gmail.com 
wrote:
  


Hello all,

Recently we have been experiencing a problem when running HE slides on our
Gemini stainer (Thermo Shandon Varistain). At the end of the run, small
water droplets or possibly air bubbles are seen on the slides after taking
the slides out of xylene. If they are coverslipped, a white haze will be
seen around the specimen after a few minutes. Any suggestions of what
causes this and how to correct it? Thank you!
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[Histonet] p16

[Nancy Schmitt]

We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container 
when received.

Comes from Dako.



Nancy Schmitt HT, MLT(ASCP)

Histology Coordinator

Dubuque, IA

*



Message: 12

Date: Thu, 7 Aug 2014 13:06:37 +

From: Burnett, Brandy 
bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org

Subject: RE: [Histonet] RE: p16

To: Weems, Joyce K. 
joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org, 
'Ronda

  Mire'  rm...@cvpath.orgmailto:rm...@cvpath.org

Cc: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

  
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu,  
sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com

  sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com

Message-ID:

  
c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.capecodhealth.orgmailto:c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.capecodhealth.org



Content-Type: text/plain; charset=us-ascii



Do you know what the dilution is from Biocare, and how many tests per vial?





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[Histonet] questions

[Nancy Schmitt]

1.  Our early (small biopsies)processor finishes at 4am.  Anything with special 
instructions (Cyto blocks, liver bxs, pros bxs, Bone Marrow cases) are done 
first. Then just by numerical as they come out of the rack.  Late processor 
finishes at 0630 and the first done are Breast bxs, sent node cases or other 
with special instructions.

2.  We find that Small bxs and core bxs do well with less than 24 hours.  Not 
sure if that is what you are looking for:)

We have a primary embedder each day.



Nancy Schmitt HT, MLT(ASCP)

Histology Coordinator

*



Message: 17

Date: Thu, 7 Aug 2014 11:54:00 -0500

From: Webb, Dorothy L 
dorothy.l.w...@healthpartners.commailto:dorothy.l.w...@healthpartners.com

Subject: [Histonet] questions

To: 'histonet@lists.utsouthwestern.edu'

  
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

Message-ID:

  
65365f35c0f2ef4d846ec3ca73e49c4302f6d319b...@hpemx3.healthpartners.intmailto:65365f35c0f2ef4d846ec3ca73e49c4302f6d319b...@hpemx3.healthpartners.int



Content-Type: text/plain; charset=us-ascii



I have a couple of questions to ask where there is no right or wrong answer, 
just curious as to the process that other labs use.

1.  After processing, how do you determine the order in which to cut and stain 
the blocks..numerical or priority driven?  2. Do you adhere to the 6-72 hours 
of fixation for breasts or make certain all breast tissue is fixed for a 
minimum time of, say, 24 hours but, of course no longer than 72?



I appreciate your responses and thanks for your time!!  I am retiring at the 
end of this year and trying to optimize some processes beforehand:).



Dorothy Webb, HT (ASCP)

Regions Histology Technical Specialist

651-254-2962




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[Histonet] P16

[Nancy Schmitt]

Actually comes from Roche..sorry about that.

Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator



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[Histonet] control slides

[Nancy Schmitt]

Good Morning-

We keep a control tissue log that documents where the tissue comes from, what 
stains it is used and has been tested for, when each block is put into use and 
exhausted and pathologist of record.  When a block goes into use, do you mark 
on each slide the date it was cut (for those control tissues that are cut 
ahead)?  How long are slides good for?

Thank you-
Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator




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[Histonet] safety shower

[Nancy Schmitt]

How often are you testing your safety shower ?  Are you always checking for 
water volume,  temperature and clarity?

I appreciate your thoughts.,

Nancy Schmitt MLT, HT(ASCP)
Dubuque, IA



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[Histonet] from saline to formalin

[Nancy Schmitt]

Do you know where there might be a reference for….”How long can a specimen be 
in saline until it should be placed in formalin or other fixative?”

Thank you

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001



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[Histonet] GATA-3

[Nancy Schmitt]

Hi All-
Looking for recommendations on the use of the antibody GATA-3 on the Leica Bond 
Max.
Are you satisfied with the results?
Thank you in advance-

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001
visit us at www.uclaccess.com




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[Histonet] RE: Histonet Digest, Vol 127, Issue 3

[Nancy Schmitt]

Hi Denise-
I would HIGHLY recommend the online program at IUPUI - we have had 4 people go 
through this program including myself.
When finished you are ready to sit for the test! It is really well done and 
they are so helpful along the way.
Contact info: Debra M. Wood, Director,
 Histotechnology Program
 Clinical Assistant Professor
 Indiana University School of Medicine
 Department of Pathology  Laboratory Medicine
 350 W. 11th St. ROOM 6002A
 Indianapolis, IN 46202
 Program Office Phone: 317-491-6410

Good Luck!
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA



_
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Denise
Sent: Tuesday, June 03, 2014 12:18 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] HTL Online Courses?

Hi all!

I have been doing massive research on this for past several days but no solid 
leads.  I am wondering about HT/HTL Histology online courses... I live in St. 
Louis Missouri and they don't offer any histology courses around here - only 
out of states.  I cannot relocate or do 1-2 years program out of state due to 
my full-time job and family reason.

Do anyone know any good Histology online courses that I can take without 
conflicting with my job?

Greatly appericated!  Thanks!

Denise Smith

smit...@kids.wustl.edumailto:smit...@kids.wustl.edu



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[Histonet] negative controls for IHC

[Nancy Schmitt]

We also stopped running negative controls more than a year ago.

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA  


From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 Abbott, Tanya
 Sent: Tuesday, February 11, 2014 12:54 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Negative Controls for IHC

 I just read in Advance Dec 2013 that there is a possibility of laboratories 
utilizing fewer QC controls to cut costs? Has anyone stopped running negative 
controls for IHC?

 Tanya G. Abbott RT (CSMLS)
 Manager Technologist, Histology/Cytology St. Joseph Medical Center 
 Reading, PA 19603-0316 ph  610-378-2635 fax 610-898-5871
 email: tanyaabb...@catholichealth.ne


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[Histonet] p16

[Nancy Schmitt]

Good Morning!

Where are you currently obtaining p16 from?

Thanks a bunch!

Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Please visit our website: http://www.uclaccess.com/




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[Histonet] slides with flimsy coverslip coming off

[Nancy Schmitt]

We received some slides (being used in a school program) - they have flexible 
plastic coverslips that are coming off and the tissue is adhered to the 
coverslip.
 Does anyone have any experience or thoughts on how we might get the tissue to 
adhere back to the slide?
Thank you!

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories




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[Histonet] wax on the floors

[Nancy Schmitt]

Happy Hump Day!

What is everyone doing to clean floors of paraffin?  Does anyone have any great 
secrets to how the floors are cleaned of the debris?

Thanks for any input!

Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories




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