[Histonet] Bringing new antibodies in house - Ki-67,P40,PAX8

2013-09-18 Thread Natalie Nagy
Hello Everyone,
  I am currently looking to bring a few new antibodies in 
house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and 
wanted to get some opinions from everyone. For P40 and PAX8; are you using a 
monoclonal or polyclonal? Also what company are you getting them from? My 
hospital deals mainly with Dako, Biocare, and Cellmarque.  We are a very small 
facility that does HIER with a waterbath, and then manual IHC pipetting with 
the old sequenza coverplate tiles. So usually we prefer concentrate for 
antibodies not pre-dilute because of the longer expiration times, and of course 
the cost. So I guess what I am asking is, what clone do you prefer. What 
company do you get it from? How much does it cost? And have you ever run it on 
a manual setup, if so how was it to work with?
 
Thanks for all the help in advance,
 
Natalie J. Nagy
Histology Supervisor
Holyoke Medical Center
 
nagy_nata...@holyokehealth.com


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[Histonet] Question about slide drying/ convection ovens

2013-01-28 Thread Natalie Nagy
Hi everyone,
  Just have a quick question for all of you out there in 
histo-land. Have you ever bought or worked with a gravity convection oven (the 
thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly 
dry all of your slides, did it keep temp?? I am thinking about buying one, and 
wanted some opinions. Or do you all prefer a real forced air slide drying oven?
Thanks in advance,
   
  Natalie Nagy (HT)ASCP
Histology Supervisor
Holyoke Medical Center
Holyoke, MA.
 


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[Histonet] ThrombinIn-Reply-To=

2013-01-28 Thread Natalie Nagy
Dear Laurie,
 We use thrombin and plasma to form our cell blocks. We order 
the Topical Thrombin 5,000 IU from King Pharmaceuticals.
Hope this helps,
Natalie Nagy (HT)ASCP
Holyoke Hospital
Holyoke, MA.


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[Histonet] Help with CPT code 88363 for archived tissue retrieval

2013-01-10 Thread Natalie Nagy
Hi everyone,
   I just have a question about CPT code 88363, first can it be 
used for pulling blocks for Oncotype DX testing, also is there a time limit on 
when this code can be used? Does it have to be within a year, a month, etc...of 
when the patient account went active?
 
Thanks for all the help,
 
Natalie J. Nagy (HT)ASCP
Histology Supervisor
Holyoke Medical Center


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