[Histonet] Collection station
I need some help. What exactly constitutes a "collection station". We are opening a satellite office and we are a Derm POL with our main lab at a different location. We plan on performing surgeries and bx and bringing the specimens back to our main lab. Does this constitute a collection station since we will be bringing the specimens back to the main office? Nicole Tatum BSH, HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clia requirements on specimens collected from satelite offices.
Histos, I have a question. We are a Derm POL and we recently opened a satellite office. I have been putting the satellite address on the pathology requisitions but do the path reports need to have that address or the address of the main lab where they were processed. Bacisly does CLIA want the address where the specimen was from, or the address where it was processed. Thank You, Nicole Tatum ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is there a Law for refusal of pathology services.
Please help, We had a patient today who had a punch bx of what is believed to be a clinical dermatofibroma. The patient stated they did not wish for the specimen to be sent for clinical testing. Our ARNP discussed the need for pathology at length and the patient stated she was a nurse and could sign a waiver denying pathology services. I have googled and gooled trying to find any specific law or statue. I can only find information pertaining to research or donated tissue. Stating a person no longer has rights or ownershipto the tissue once consented and removed, but this case is not for research. Could someone pls share an actual law with me. Seems silly to consent to the surgery but not to the diagnosis. Im not sure what to do at this point. Have them sign a document on our company letterhead stating there denial of services? Hold the tissue hold long? Accession it but only do gross description? Charge anything? Any thoughts or imformation would be greatly appreciated. Nicole Tatum HT BSH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shandon HistoCentre II question
I know most embedding machines put out heat due to the block warming chamber, paraffin vat and compressor of cooling consol. But those of you who have experience with this machine, do you think it seems hotter than other or does it have issues with retaining a constant temperature? Any thoughts.. Nicole Tatum, HTL ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Softening toenails procedure
Hi, I need a good procedure for softening toe nails to run on HE. I have used Nair and KOH. Anyone have a good procedure that works well that they would like to share. Thanks, Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] POL labs
Let me start by sharing this: Definition of FREE ENTERPRISE : freedom of private business to organize and operate for profit in a competitive system without interference by government beyond regulation necessary to protect public interest and keep the national economy in balance. Key Word being For Profit. Health care is a commodity that is bought and sold and the medical industry is big bucks for our economy. So what if a POL is for profit, so are some hospitals, pharmaceutical companies, pharmacies, and the local gas station. My point being is, just because a POL is for profit does not mean that the facility does not offer the same quality of care as a national laboratory who is also seeking profit. So, as far as Im concerned the Doctor, owner, or medical director is able to bill for any test he performs in his facility that is currently licensed and regulated. I really dont think the setting should be a factor. We all will see changes and cuts. I do not believe this thread has any thing to do specifically with the election. Besides it doesnt really matter what side of the fence your on. Cuts are comming, dare I say rationing. Even if socialized medicine does not get passed and Romney wins, Medicare will have to decrease its allowable payouts each year. I personally am more worried about what that will mean for our payscale. For those of you who dont know me, I DO work in a POL lab. Im not bias, but I don't think the location of my lab is relative to the fact that it shouldn't be allowed to exist because its for profit. Just my thought. Happy Halloween to all. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unregistered techs
LOL, I dont have much to say about this one. Like you Kim I have a fl HTL license and an HT ASCP. I have two classes left before I complete my BSH and will be able to sit for my ASCP HTL. I have 12yrs of experience but that is not reconginized in the eyes of licensure for ASCP. I do believe my skill level should determine my licensure status not the degree I hold. Because I promise every class im taking rt now means nothing towards my field. The word histology has not even be written in any of the books im studying. I think OJT techs are just as qualified as any person who completed an online programs, if not more so. I completed an online histology program and learned my skills from other people in the bizz with alot of experience who were willing to train me and be patient. Never one time did I question their, experience, licensure, or training. The OJTs carried Histology through the transisition of licensure and passed the skills and knowlegde on to us young bucks to carry the torch. That is all...lol Nicole Tatum HT ASCP (hahaha soon to be HTL ASCP) :)~~~ LOL, I was waiting for this thread to turn into a fist fight. I knew it would. A Fl Licensed HTL is someone who oviously had to take more test than a ASCP certified tech if they got it from 19 years ago. How do I know, I'm one of those who had to take all those test way back then. Florida is pretty stringent who can do what with what. Not as much as they used to be, because now the only route is through ASCP. You will not work in a hospital as a histologist without a Fl License. Maybe in a exclusive provider private office, but that is the only exception. As far as Monkeys. Whew! I think that's whats wrong with our profession, one thing anyway. Too many people veiw it that way. Personally I wish the Histo programs would go back to teaching on campus with using the MLT course comparison. Not to bash anyone who is either involved or has been involved with the internet programs that have popped up everywhere, but I'm not seeing the same level of technical skill or knowledge come out of these programs. I'm sure there are many super students who do get it, but many times they are pushed through on thier short rotations and used as gophers etc and dont have the skills even sometimes to cut a block. And forget about knowing what a good control for a GMS is. I'd like to see our profession go back to min AS degrees in Histology. The certification only have hurt us and the pay scale is changing I feel because of it. Also, if you really want to promote our field and improve it, be supportive of your state going to a license for our practice. ASCP supports states requiring license. Thats my 2cents for the day. Happy Friday! :) Kim D From: William Chappell cha...@yahoo.com To: Davide Costanzo pathloc...@gmail.com Cc: histonet histonet@lists.utsouthwestern.edu Sent: Thursday, May 24, 2012 7:02 PM Subject: Re: [Histonet] Unregistered techs I have respected Jay's input in the past, but I too must say something. Without realizing it, and by stating his opinion in a horribly crass way, Jay has touched upon an important truism. There are two types of histologists, those that have a job that pays the bills, and those who have a career in which they thrive. Neither are better than the other, both are needed. I suspect, however, that the majority of Histonetters -- especially avid contributors are in the latter group. I know I am. Histotechs who approach histology as a job, go into work, embed, cut, stain and go home. they are excellent techs, but are just not committed to expanding the field or doing more than is needed to provide the pathologist with a perfect slide. Jay refers to these people as no better than trained monkeys. That is a horrible insult with a small (very small) grain of truth. One day those histologists will be replaced by a mechanical/robotic process. The march of progress is unstoppable. The career histologist has a much longer life span however. We analyze and troubleshoot problems. We understand or endeavor to learn the organic chemistry of stains. We know EXACTLY how a Rabbit Monoclonal antibody is made. We know more about the practice of histology than ANY pathologist. We invent and develop antibodies and special stains. And we conceptualize and perfect the instruments that will replace the first group in the future. Jay, that is why so many are offended. We don't do this simply because it is a good paycheck. We are histologists because we are professionals who choose this career. You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice, not accident On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: I'm sorry - I cannot let this rest. The comment: we are just as much needed
[Histonet] Fwd: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs
Original Message Subject: Fwd: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs From:blondi33...@aol.com Date:Tue, May 8, 2012 3:57 pm To: nic...@dlcjax.com -- -Original Message- From: nfe1244 nfe1...@aol.com To: seaglstein seaglst...@gmail.com; pam p...@dlc.net; blondi1 blondi33...@aol.com Sent: Sun, May 6, 2012 9:06 am Subject: Fwd: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs good news! -Original Message- From: American Academy of Dermatology Association nore...@aadassociation.org To: N. Fred Eaglstein; DO; FAAD nfe1...@aol.com Sent: Fri, May 4, 2012 5:58 pm Subject: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs If you can't see the images in this email, please click here. This weekâs headlines: Indoor tanning bed labeling legislation introduced, fails to include FDA reclassification House and Senate Committees continue working on prescription drug, medical device legislation AADA provides comments to the FDA regarding biosimilars AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs Dermatology societies to collaboratively share recently approved AUC for Mohs surgery with payers Vermont becomes second state in the nation to ban tanning for minors California patient safety bill moves swiftly through state assembly Cosmetic tax proposals stripped from California bills Tanning bill endorsed by Missouri House of Representatives Mississippi enacts ÂPatientÂs Right to Informed Health Care Choices Act Maryland enacts board certification disclosure requirements SkinPAC to host fundraiser in Chicago Register now for the 2012 AADA Legislative Conference, Sept. 9  11, Washington D.C. Congressional action Indoor tanning bed labeling legislation introduced, fails to include FDA reclassification On April 19, Sens. Jack Reed (D-R.I.) and Johnny Isakson (R-Ga.) introduced S. 2301, the Tanning Transparency and Notification Act of 2012 which calls on the FDA to enhance indoor tanning bed labeling requirements based on recommendations the agency made as a result of the TAN Act of 2006. The AADA has been working closely with Sen. ReedÂs office to introduce a Senate companion bill to H.R. 1676, the Tanning Bed Cancer Control Act, which calls on the Food and Drug Administration (FDA) to reclassify indoor tanning beds, however Sen. Reed introduced his new Senate legislation removing the AADA-supported FDA reclassification language and leaving only the enhanced labeling portion of the bill. The AADA sent a letter to both senators stating the importance of including language calling on the FDA to reclassify tanning beds, in addition to the enhanced labeling requirements that, alone, do little to deter the use of indoor tanning beds. House and Senate committees continue working on prescription drug, medical device legislation The House Energy Commerce Committee and the Senate Health, Education, Labor, and Pensions (HELP) Committee are working on a bipartisan effort to reauthorize prescription drug and medical device user fee legislation. The resulting bills are expected to come to the House and Senate floors for action sometime in June. Both the House and Senate draft bills include language to address the ongoing prescription drug shortages that physicians across the country are facing and propose solutions to mitigate future shortages. Additionally, the Senate HELP Committee version currently includes legislation (S. 2301) introduced by Sens. Jack Reed (D-R.I.) and Johnny Isakson (R-Ga.) calling on the FDA to enhance indoor tanning bed labeling requirements based on the agencyÂs recommendations (see story above). As the Committee process moves forward, the AADA is monitoring the legislation and urging Congress to also include language calling on the FDA to reclassify indoor tanning beds. Federal agency focus AADA provides comments to the FDA regarding biosimilars On May 11, the Food and Drug Administration will convene a public hearing regarding biosimilars. The hearing will include conversations on naming, labeling, and pharmacovigilance of these new therapies. In anticipation of the hearing, the AADA has submitted comments to the agency highlighting our continued concerns regarding naming and pharmacoviligance issues. The Academy urges the agency to provide unique non-proprietary names for all biosimilars to reduce any confusion with the reference biologic products. An update on the May 11 meeting will appear in the next issue of Dermatology Advocate. Private payer activity AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs Aetna has
Re: [Histonet] RE: Qualifications for grossing
Yupp he strikes again. Joanne, I strongly agree with your perspective. Many Techs do not have formal expensive educations and have sat on the bench for many years and eventually became grandfathered in. Those techs are the life blood of pathology. It has only been in recent years that licensure has become a larger part of health care requiring personal to obtain certification to hopefully increase patient care. But, this argument is becoming a thing of the past, because CLIA, CAP, JOCA have set standards that personal must meet regardless of the extensive OJT. I am qualified to gross based on these accrediators standards. It is others opinions that think these standards are weak. If the argument is greed, than people should understand that employee payroll is the highest cost within a laboratory so to help cut cost to our bankrupt health care system, why not pay a Histologist who is clearly qualified to do a job they have been doing since the beginning of pathology. The pathologists assistant profession began in 1969 with a pilot training program at the Veterans Administration Medical Center (VAMC) in Durham, North Carolina. In 1856 William Perkin discovered the dye mauve that was used in the early 1860s by F W B Benke of Marlbery. Joseph Janavier Woodward, a surgeon in the US Army, used fuchsine and aniline blue to stain human intestines. Paul Ehrlich realized that the chemical dyes obtained from coal tar did not simply color cells but combined with the chemical elements within them to form new substances. The Swiss chemist Friedrich Miescher, in 1869 used aniline dyes to examine the cell nucleus. In 1875 Carl Weigart, Ehrlich's cousin, demonstrated the fuchsine derivative methyl violet stained bacteria as opposed to tissue. The first histologist, Marcello Malpighi (1628-1694), an Italian anatomist, is in fact considered the true Father of Histology. 1819, A. Mayer created the term Histology. In the sequence of the previous word tissue, made use of two classical Greek root words (histos = tissue and logos = study So, my point is I do believe Histo's have been involved since the very beginning. We as a profession have a combined experience well beyond that of any formal education. Last thought, and I quote, I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in health care? Mistakes should happen in school, not with a real, live patients tissue. This statement clearly conflicts with all aspect of becoming a medical professional. Our state/government funded hospitals employ thousands of residents each year who treat thousands of indigent and paying patients. This is their school. Histologist do interships within hospitals to get training. This is their school to. Nurses, MLT's, MA's, everyone in health care learns the actual (beyond books)trade from watching and working with skilled persons who have many years of experience. They would not place a student with a person who has a degree but no experience. My education qualifies me to be trained by anyone in my field of pathology, and I should be used where my skills will best serve my department and increase patient care. Each one of us serves a purpose and is valuable, no matter what job we perform with what amount of education. The law is weeding out those who are no longer qualified to work in out field, they set the standard. Let's let them make the decisions on who is qualified to do what and stick together to ensure its fair to each one of us. Can't we all just get along...hehehehehe Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Qualifications for grossing
Im sorry you feel that way about me. There is nothing snide here. I respect your opinion and have no foul words for you. Im happy that you have earned your education. I currently am in school trying to further mine, and I belive an education is so important. To a person and a profession. Have a wonderful day. Nicole Tatum, HT ASCP Try to keep your snide remarks quiet, and respond with some degree of respect. We will not always agree, but there is no strike there. You dislike me,, that's fine. But keep your personal comments to yourself. If you can be that mature. Sent from my Windows Phone From: Nicole Tatum Sent: 4/25/2012 12:09 PM To: Joanne Clark; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Qualifications for grossing Yupp he strikes again. Joanne, I strongly agree with your perspective. Many Techs do not have formal expensive educations and have sat on the bench for many years and eventually became grandfathered in. Those techs are the life blood of pathology. It has only been in recent years that licensure has become a larger part of health care requiring personal to obtain certification to hopefully increase patient care. But, this argument is becoming a thing of the past, because CLIA, CAP, JOCA have set standards that personal must meet regardless of the extensive OJT. I am qualified to gross based on these accrediators standards. It is others opinions that think these standards are weak. If the argument is greed, than people should understand that employee payroll is the highest cost within a laboratory so to help cut cost to our bankrupt health care system, why not pay a Histologist who is clearly qualified to do a job they have been doing since the beginning of pathology. The pathologists assistant profession began in 1969 with a pilot training program at the Veterans Administration Medical Center (VAMC) in Durham, North Carolina. In 1856 William Perkin discovered the dye mauve that was used in the early 1860s by F W B Benke of Marlbery. Joseph Janavier Woodward, a surgeon in the US Army, used fuchsine and aniline blue to stain human intestines. Paul Ehrlich realized that the chemical dyes obtained from coal tar did not simply color cells but combined with the chemical elements within them to form new substances. The Swiss chemist Friedrich Miescher, in 1869 used aniline dyes to examine the cell nucleus. In 1875 Carl Weigart, Ehrlich's cousin, demonstrated the fuchsine derivative methyl violet stained bacteria as opposed to tissue. The first histologist, Marcello Malpighi (1628-1694), an Italian anatomist, is in fact considered the true Father of Histology. 1819, A. Mayer created the term Histology. In the sequence of the previous word tissue, made use of two classical Greek root words (histos = tissue and logos = study So, my point is I do believe Histo's have been involved since the very beginning. We as a profession have a combined experience well beyond that of any formal education. Last thought, and I quote, I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in health care? Mistakes should happen in school, not with a real, live patients tissue. This statement clearly conflicts with all aspect of becoming a medical professional. Our state/government funded hospitals employ thousands of residents each year who treat thousands of indigent and paying patients. This is their school. Histologist do interships within hospitals to get training. This is their school to. Nurses, MLT's, MA's, everyone in health care learns the actual (beyond books)trade from watching and working with skilled persons who have many years of experience. They would not place a student with a person who has a degree but no experience. My education qualifies me to be trained by anyone in my field of pathology, and I should be used where my skills will best serve my department and increase patient care. Each one of us serves a purpose and is valuable, no matter what job we perform with what amount of education. The law is weeding out those who are no longer qualified to work in out field, they set the standard. Let's let them make the decisions on who is qualified to do what and stick together to ensure its fair to each one of us. Can't we all just get along...hehehehehe Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ACMS perspective on Aetna's new requirments.
This is a letter From the American College of Mohs Surgery in reguards to Aetna letter for CAP requiremnet. April 5, 2012 I read your letter of March 23, 2012, outlining Aetnas new requirement for CLIA and CAP certification for certain pathology services with dismay. I am very concerned these requirements could lead to lower cure rates, increased tissue loss and scarring, and even unnecessary deaths in your patients with problem skin cancer with Aetna insurance. CAP certification will be almost impossible to achieve for small Mohs Micrographic surgery frozen section laboratories. These labs can only be CAP certified if directed by an anatomic pathologist or dermatopathologist. Mohs surgeons, who have extensive additional training in pathology, and training to run their laboratories, do not fit into either niche. Mohs surgeons deal with the most difficult and recurrent skin cancers. Please find attached a copy of the CPT coding assistant from 2008 describing Mohs surgery, and detailing what services are included in it. With your new restrictions, immunohistochemistry (CPT code 88342) cannot be billed with Mohs surgery for malignant melanoma and spindle cell tumors. This will restrict the use of Mart 1 immuno stains and other special cytokeratin stains. This will result in lower cure rates and an increased number of deaths. These stains on additional frozen sections are not part of Mohs surgery, and are appropriately billed for separately. In addition, Mohs surgeons will not be able to bill for the occasional special stains on frozen sections (CPT code 8814) such as oil red O on a sebaceous carcinoma, or toluidine blue (in addition to the hematoxylin and Eosin stained frozen sections) to clarify an area of inflammation. Performing these special stains on ambiguous frozen sections often saves the patient additional surgery and tissue loss, and saves Aetna money, because another stage of Mohs surgery is avoided. These additional stains are not part of the Mohs surgery and are appropriately billed for separately. Denial of these CPT codes may result in more tissue being removed unnecessarily, lower cure rates, higher recurrence rates, and potentially deaths. Quality control of the frozen section laboratory is crucial, and mandatory for CAP lab approval. This quality control involves processing of confirming formalin sections off the frozen blocks of tissue is commonly performed in Mohs surgery laboratories for quality control and confirmation. This will no longer be a billable service (CPT code 88305) per your letter. This directly contradicts CAP own recommendations for the follow up processing of frozen sections (see attached). These confirming formalin sections are not part of Mohs surgery and are separately billable. Your decision not to cover code CPT 88305 makes it impossible, or at a minimum, fiscally prohibitive, to maintain quality control and to even consider complying with the new CAP accreditation you are demanding in the same letter. From the perspective of the American College of Mohs surgery, our patients could continue to receive the best quality of care, and the overall cost to Aetna may actually be lower (by avoiding additional stages of Mohs surgery), if CPT Codes 88314, 88305, and 88342 were exempted for providers who also bill the Mohs surgery CPT codes 17311 or 17313. Sincerely Brett Coldiron, M.D., F.A.C.P. President American College of Mohs Surgery ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] In House Labs in WSJ
Agreed , but the idea in recent health care has been early detection. So as technology increased, more diagnostic test were ordered. But, that is not only pathology, its micro, radiology, ultrasound, chemistry, etc. These early test did drive cost up, but also saved cost. Its kinda no win. If a radical tumor was detected early it could be removed by surgery and the patient could possibly still live a healthy cancer free life. If not detected, the patient could suffer through chemo and expensive radiation, and expensive hospice. Leaving the family devistated with medical bills and the loss of a loved one that a simple diagnostic test could have detected. Also, there is a huge problem with the malpractice suits in our country. If the pateint did suffer or die because a simple test was not ordered that could save their life the physician is held responsible and sued and could possibly lose his license and career. If less test are the answer to cutting cost to our rising health deficit, then doctors should be more protected when they make choices not to order tests that could save your life. Despite cost, in court they will argue it was a simple inexpensive test that could have saved his or her life. The physician is charged with protecting a patients health and he needs tool to do that. Tools that are being taken because they are unaffordable. We must learn how to manage our resources at every level. I for one would be devistated if I had cervical cancer because my OB did not submit a specimen when my pap came back as abnormal. I would be willing to pay the path fee out of pocket to have an answer. But, that's also part of the problem. People do not want to pay for services they recieve. But, they have a really nice flat screen and iphone. This economic crisis is a result of the public and health professional and gas prices, etc. We must stick together and come up with ways to still use diagnostic test effectively. They do save lives and save money, maybe on a small scale compared to those who are not diagnosed with any condition. Our current health care model has been based on detection and prevention. It will have to change for our industry to survive. Resouces will have to be rationed but I fear it is being given the title of over-utilization instead. As current tests decrease and physician are pushed to order less; I fear there will be an increase of misdiagnosises and an increase in malpractice suits. Its becomming scarry out there.. This change will effect each one of us. Nicole Tatum HT ASCP On 4/10/2012 5:33 PM, Kim Donadio wrote: Less screening = fewer biopsies = less revenue = less prostate cancers caught early = more deaths to prostate cancers. Would you not agree? No. There is very good scientific evidence that screening does not increase survival rates but it does drive up costs and unnecessary surgery and related complications. I can send the papers from NEJM if you like. Geoff And for all those advocating closure of private labs, do you also feel the same way about private pathologist owned labs who reep the benefits of getting all the out PT work from affiliated physicians while they also get a fee to serve as medical directors of hospital labs and get the pc portion of hospital work of which they can order as many test they want so they get the pc portion while the hospital gets the tc and all the big bills associated with doing the test making it hard on tax payer as well because so much in a hospital is already subsidize by the gov. Is what you really want is to have all pathologist as employees of the hospitals? And have the hospital bill global. And a few walmart like reference labs I'm just curious as to the exact position of some on here. Thanks Kim Sent from my iPhone On Apr 10, 2012, at 2:39 PM, Morken, Timothytimothy.mor...@ucsfmedctr.org wrote: Not surprising since our health care system is biased to pay for tests and treatments, not results. On top of this there are serious questions as to whether the PSA screening that leads to biopsies is useful in the long term. There is a recommendation out there to stop PSA screening for most men since it is largely non-specific. That test is what leads to the biopsies. Less screening = fewer biopsies = less revenue. Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Tuesday, April 10, 2012 11:22 AM To: Histonet Subject: [Histonet] In House Labs in WSJ The Wall Street Journal served up a timely article for us. You'll see both sides of the argument below. One side is right. DLS HEALTH INDUSTRY April 9, 2012, 7:22 p.m. ET Prostate-Test Fees Challenged By CHRISTOPHER WEAVER Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday
[Histonet] Aetna in-house
I have reviewed my letter from Aetna and it reads, This change is consistent with the Center for Medicare Medicaid Services recongnition of CAP as an approved accreditation organization for non-hospital anatomic pathology testing. I have called Medicare and they state that they have made no changes and CLIA is the only enitity they require to recieve reimbursment for path services reguardless of location. If that was the case I would definitly have to get my CAP. Because if Medicare does, it you know they will all follow suit. So, to any smaller lab. Medicare has not changed to CAP and CLIA remains the only certification you need. Nicole Tatum HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Aetna and In-Office Lab Accreditation
Money is at the root of all finicial decisions, in-house labs and hospitals. There are many over utilization of resources within the health care field. Many gallbladder surgerious are performed unneccesarly by general surgeous who's practice are within hospitals walls. Tonsilectomy. etc. How are those specimens not self reffered to the hospitals AP lab. David you made the comment about specialities staying with there specialty and not branching out. A dermatopathologist specializes in derm specimens so why is it so far fetched that he would read derm specimens from all sources, hospitals or in-house labs. My in-house lab has a higher turn around rate, lower overhead, and cuts courier fees out. We also do a service to our patients by allowing them one stop shopping. We can service all there needs and they do not have to have multiple appointments at different facilities. This cuts down on their copay and billing from multiple doctors. Also, it would cost more for a person to have Mohs surgery in a hospital setting. As we all know cost are higher at a hospital because they have higher overhead. The hospital is self reffering when they let a surgery center or group be affiliated with them. The surgery center was allowed to join the hospital so the hospital could reep the revenue generated and process their specimens. Either way, we are all joined by a common form of employment, and one facility is not better than another. My field provides jobs and creates revenue just like yours. Insurance company are going to make changes to try and make revenue during this change into OBAMA CARE. Remeber we are not the enemy they are. Who are they to dictate how my company runs. Insurance companies have to much power and the decisions they force us to make do not always provide the best patient care. And that is the ultimate goal for any provider, to give best patient care right? This is just another hurdle we all must jump through in these comming changes. I vote we stick together and try our best to protect all our jobs. Wasnt that long ago that each of us we trying to get pay increases and bring the importance of our jobs to the fore front of pathology. The financial squeeze of the helath care system is going to be felt by all. Histology, pathology, radiology, cytology, we all must do our best to role with the punches and ensure quality care and our incomes, as well as our field, reguardless of location. Nicole Tatum, HT ASCP Thank you for that. How are things at Hartford Hospital? One of my favorite places, rotated there many years ago. Very impressive facility! Is Dr. Ricci still there? On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun rcar...@harthosp.org wrote: This was released today. Richard Statline Special Alert: New Evidence Links Self-Referral Labs to Increased Utilization, Lower Cancer Detection Rates Study co-funded by CAP Appears in April 2012 Issue of Health Affairs April 9Self-referring urologists billed Medicare for nearly 75% more anatomic pathology (AP) specimens compared to non self-referring physicians, according to a study published today in a leading health care policy journal. Furthermore, the study found no increase in cancer detection for the patients of self-referring physicians-in fact, the detection rate was 14% lower than that of non self-referring physicians. These findings, from an independent study co-funded by the CAP, provide the first clear evidence that self-referral of anatomic pathology services leads to increased utilization, higher Medicare spending, and lower rates of cancer detection. The study, led by renowned Georgetown University health care economist Jean Mitchell, PhD, will appear in the April 2012 issue of Health Affairs and is now available on the journals website. Daniel Schneider dlschnei...@gmail.com 4/9/2012 4:47 PM This is all about the money. The rest is rationalization. The reason a group of non-pathologist physicians opens an in-house pathology lab and hires an employee pathologist is first and foremost to harvest profit from pathology reimbursement. Be a fly on the wall in the partners' meetings and you would know that's what they are talking about. To suggest otherwise is disingenuous. And the implication that the generalist anatomic pathologist is unqualified to be signing out skins, prostates, GI's and whatever is reprehensible. This is not cardiac bypass surgery, and AP pathologists *are* trained to do all of the above. I eagerly defer to subspecialty expert consultants as needed, but most of the time they're not needed. Hospital labs that see few, if any skins, prostates, GI's, are only in that pickle because of the cherrypicking they've already been subjected to. *in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source
Re: [Histonet] Aetna and In-Office Lab Accreditation
Well Said To suggest that any physician who goes into private practice and has their own lab is any more of a money hound than any other physician at a hospital would also be disingenuous . And of course this is about money. It's about one group of people trying to get another group of people out of the lab business because they want that money. It's also about the government squeezing insurance companies into these more stringent regulations. Now I'm not against more stringent regulations but I do find it offensive of how they are going about it. The little guy will take the hits on this one. I guess what they want is a bunch of walmart like labs. Private practices serve a patient care cause just as hospital labs do. They all make a diagnosis. They all deserve to be paid. My 2 cents Sent from my iPhone On Apr 9, 2012, at 4:47 PM, Daniel Schneider dlschnei...@gmail.com wrote: This is all about the money. The rest is rationalization. The reason a group of non-pathologist physicians opens an in-house pathology lab and hires an employee pathologist is first and foremost to harvest profit from pathology reimbursement. Be a fly on the wall in the partners' meetings and you would know that's what they are talking about. To suggest otherwise is disingenuous. And the implication that the generalist anatomic pathologist is unqualified to be signing out skins, prostates, GI's and whatever is reprehensible. This is not cardiac bypass surgery, and AP pathologists *are* trained to do all of the above. I eagerly defer to subspecialty expert consultants as needed, but most of the time they're not needed. Hospital labs that see few, if any skins, prostates, GI's, are only in that pickle because of the cherrypicking they've already been subjected to. *in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source of employment for our professions is a good thing.* Really? The jobs follow the specimens. Given the same number of specimens, there's the same number of jobs, more or less, just under different circumstances and in different locations Unless you're suggesting that in-office labs will generate increased specimens, and thus increased jobs though overutilization, i.e. excessive numbers of unnecessary biopsies and abuse of the patient and the taxpayer. In which case I have to say there's a grain of truth. And the truth hurts. And it's not a good thing. None of this should be taken as criticism of histotechs and pathologists who find themselves working in an in-office lab. I know there's bills to pay, families to take care of, and god knows it's hard for a pathologist to find a job these days with the numbers our residency programs keep churning out (but that's another rant...). Dan Schneider, MD Amarillo, TX On Mon, Apr 9, 2012 at 12:52 PM, jdcoch...@aol.com wrote: Histonetters: In-office AP labs provide a valuable service to the practices they serve by facilitating 1) better communication between pathologists and ordering clinicians, 2) quality metrics that are practice-specific, and 3) high volume, sub-specialization for both histotechnologists and pathologists. In other words, the more of one type of histopathology a lab does (e.g., skin, prostate, GI), the better it gets. Most people would not think of having their cardiac bypass surgery done at a community hospital doing 50/year; you want to go where more than 500/year are done. In histopathology, the kinds of volume you want are in the thousands for each tissue type. Many hospital labs do little skin or prostate histology anymore. Many sub-specialty in-office AP labs may do thousands of cases of one tissue type every year. Aside from that, in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source of employment for our professions is a good thing. This requirement by an insurer for accreditation will help to validate these in-office AP labs' commitment to quality and put them on the level with their hospital counterparts. John D. Cochran, MD, FCAP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Aetna and In-Office Lab Accreditation
Really, An undertaker. Yea, theres definately a conflict here, you. No since in wasting my time. Nicole Start with reading Dr. Schneider's post. Then read Richard Cartun's post. Those should deal will what you are talking about very well. These in-office labs should not exist, for the very same reason the undertaker is no longer the ambulance driver. There is a very real, and significant conflict of interest. Sent from my Windows Phone From: Nicole Tatum Sent: 4/10/2012 6:45 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation Money is at the root of all finicial decisions, in-house labs and hospitals. There are many over utilization of resources within the health care field. Many gallbladder surgerious are performed unneccesarly by general surgeous who's practice are within hospitals walls. Tonsilectomy. etc. How are those specimens not self reffered to the hospitals AP lab. David you made the comment about specialities staying with there specialty and not branching out. A dermatopathologist specializes in derm specimens so why is it so far fetched that he would read derm specimens from all sources, hospitals or in-house labs. My in-house lab has a higher turn around rate, lower overhead, and cuts courier fees out. We also do a service to our patients by allowing them one stop shopping. We can service all there needs and they do not have to have multiple appointments at different facilities. This cuts down on their copay and billing from multiple doctors. Also, it would cost more for a person to have Mohs surgery in a hospital setting. As we all know cost are higher at a hospital because they have higher overhead. The hospital is self reffering when they let a surgery center or group be affiliated with them. The surgery center was allowed to join the hospital so the hospital could reep the revenue generated and process their specimens. Either way, we are all joined by a common form of employment, and one facility is not better than another. My field provides jobs and creates revenue just like yours. Insurance company are going to make changes to try and make revenue during this change into OBAMA CARE. Remeber we are not the enemy they are. Who are they to dictate how my company runs. Insurance companies have to much power and the decisions they force us to make do not always provide the best patient care. And that is the ultimate goal for any provider, to give best patient care right? This is just another hurdle we all must jump through in these comming changes. I vote we stick together and try our best to protect all our jobs. Wasnt that long ago that each of us we trying to get pay increases and bring the importance of our jobs to the fore front of pathology. The financial squeeze of the helath care system is going to be felt by all. Histology, pathology, radiology, cytology, we all must do our best to role with the punches and ensure quality care and our incomes, as well as our field, reguardless of location. Nicole Tatum, HT ASCP Thank you for that. How are things at Hartford Hospital? One of my favorite places, rotated there many years ago. Very impressive facility! Is Dr. Ricci still there? On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun rcar...@harthosp.org wrote: This was released today. Richard Statline Special Alert: New Evidence Links Self-Referral Labs to Increased Utilization, Lower Cancer Detection Rates Study co-funded by CAP Appears in April 2012 Issue of Health Affairs April 9Self-referring urologists billed Medicare for nearly 75% more anatomic pathology (AP) specimens compared to non self-referring physicians, according to a study published today in a leading health care policy journal. Furthermore, the study found no increase in cancer detection for the patients of self-referring physicians-in fact, the detection rate was 14% lower than that of non self-referring physicians. These findings, from an independent study co-funded by the CAP, provide the first clear evidence that self-referral of anatomic pathology services leads to increased utilization, higher Medicare spending, and lower rates of cancer detection. The study, led by renowned Georgetown University health care economist Jean Mitchell, PhD, will appear in the April 2012 issue of Health Affairs and is now available on the journals website. Daniel Schneider dlschnei...@gmail.com 4/9/2012 4:47 PM This is all about the money. The rest is rationalization. The reason a group of non-pathologist physicians opens an in-house pathology lab and hires an employee pathologist is first and foremost to harvest profit from pathology reimbursement. Be a fly on the wall in the partners' meetings and you would know that's what they are talking about. To suggest otherwise is disingenuous
Re: [Histonet] Slide Brite
I have used this product for about 10yrs and love it. It has no fumes and no foul odor like the orange smelly stuff which gives me the worst headache. I use it in my processor. To deparrafinize and in my routine stain line. It works great with special stains to. I have not used it for IHC since the lab I work in does not deal with that test. It is non toxic as well. I purchase my supply from Chad at Mercedes Medical. P.S. There are few draw backs. I feel that the solution has to be changed more often than xylene as it becomes saturated faster. It also does not remove coverslip as well as xylene. Nicole Tatum, HT ASCP Hello. I saw a post this morning about Slide Brite substitute for Xylene and would like to get your opinion(s) on the product. Thank you, Nanne Marsh HT (ASCP) Histology Specialist II 1000 E 50th Street Kansas City, MO. 64110 (816) 926-4305 n...@stowers.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Respirators and Routine Histology
I also do not wear any type of respirator. Not at any point of my day. I annually wear a formalin badge to test for exposure rate, but thats it. I gross under a fume hood and I use Slide Bright instead of xylene. It does not have any fumes or noxious odor and is non toxic. My stain line is also contained under a fume hood. No one ever smells or complains about my fumes, unless im changing the processor, they tend to smell the alcohol then. Mask are a required safety supply, and I do believe in some situations a respirator may be needed, but it is ultimately up to the tech. Besides the lab should have adequite ventilation that a respirator should not be needed for the entire shift, maybe during specfic tasks with high fumes. Nicole Tatum, HT ASCP Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histo Aide Duties
Most professional labs require a person who has completed an accrediated histology programs, but CLIA states that a person can work within a histology lab if they have a combination of 12hrs of college chemistry or biology. In any combination. Then that person needs to be trained in each area they will work in and be signed off on by the directions as being able to perform tasks. To say that an unlicensed person working in histology can not coverslip is silly. I agree that this is the last chance to verify stain quality as well as tissue quality, but, before licensure there were thousands of OJT employees filling our histology positions. Many of whom trained each of us to have a critical eye when we were students performing our internships. If the person is working in ur labs as an unlicensed assistant, you must of had some confidence in them or you would not have them working in your lab. The evolution and technical skill of our trade has been taught from one person to another, at a time when licensure was not as important. That being said, licensure is now becomming critial to work in our field. But that does not mean that currecntly there are many unlicensed competent persons working in histology. They work as Mohs techs , and prep techs, and gross techs, etc. I would hope that a nonlicensed Mohs tech would know how to access their slides and coverslip effectively. My point, all persons working in our field contribute and get the job done, despite their licensure situations. They could still be very competent individuals if training by a patient person who is willing to educate others. Nicole Tatum HT ASCP ASMS I don't want to seem nit picky, but I tend to strongly agree with Rene's point about coverslipping. It is not really the act or task of coverslipping to me, but rather the fact that it is one of the last opportunities to assess the slide for technical quality, overall presentation, and information accuracy before passing out. I feel that this should involve microscopic assessment, and also the judgment that arrives from knowledge and experience to determine if the slide is acceptable. I don't think it would be reasonable to expect all of this to occur with a person who has not had the benefit of training. many errors could be allowed out of the lab if labels are just put on without really looking at the slides, just think of the impact on perception of quality, accuracy and competency of histology. Most times, it is the oops that seem to stay in memory, and not the bulk, which go out fine. Not worth it to me.Joelle http://www.linkedin.com/in/joelleweaver Date: Wed, 7 Dec ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: cassette marker
I absolutely love mercedes medical pens called platunium line mer marker. They are smudge prrof and stay on. Love them. Im sure if you called CHAD at mercedes he could send you a free sample. Nicole Tatum Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000. Best for staying on but sensitive to writing on surfaces that are not absolutely dry. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Saturday, December 03, 2011 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette marker Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problems with Frozen tissues
Igor, I perform Mohs which is a frozen section procedure. There are a couple of things that could help you. You do not have to leave the slides out to airdry for an HE only do this is a specfic procedure requires it. Do not places slides strait into water. As Kim, stated some people use a fixative prior to staining. I place slides into 95% alcohol and then they are rinsed. The next thing to consider is how thick you are cutting the tissue. It can be difficult to keep thick, fatty, or tissue with alot of cartiladge on the slide. So cut as thin as possible. Ok next is the oct or mounting media ur using. In between each section you should wipe the oct. OCT is water solauble. If the tissue is placed over OCT on the slide it will definitly wash off. So if you are trying to put multiple sctions on a slide, Place enough room between them so the tissue is directly placed on the clean surface of the charged slide, or wipe excess oct in between sections. Last note. If you have automatic stainer and it has an aggitate function.Turn it off. Stain the slides as gently as possible. Hope this helps, Nicole Tatum HT ASCP Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. Kim From: Igor Deyneko igor.deyn...@gmail.com To: Histonet@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:56 AM Subject: [Histonet] Problems with Frozen tissues I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do HE and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] background checks required by AHCA
I am currently filing out my ahca renewal application. There are many changes and the form and I do not find it user friendly. Anyways here my question: Medical directors and chief finicial officers or any person who stands to gain profit must undergo a level 2 background screeening. On the new app is states all empolyees and health care providers. I called acha licensing division and they said only directors and so forth. But, on ahca website statue 408(something, ill have to get exact number) when into effect in 2010 that says all employees and newly hired employees must underground background screening. So does all the arnp, pa, ht, and ma's need to be screened because we have a lab? Nicole Tatum, HT ASCP http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_WhoRequiredToBeScreened.pdf taken directly from ahca site: Employees and Contractors employed before August 1, 2010 Every employee/contractor must attest to meeting the requirements of this chapter and agreeing to inform the employer immediately if arrested for any of the disqualifying offenses while employed by the employer. [Section 435.05(2)]. This attestation must be maintained in the employees personnel file. You may use the Affidavit of Compliance with Background Screening to satisfy the attestation requirement. If an employer becomes aware that an employee/contractor has been arrested for a disqualifying offense, the employer must remove the employee/contractor from contact with any vulnerable person that places the employee/contractor in a role that requires background screening until the arrest is resolved in a way that the employer determines that the employee/contractor is still eligible for employment/contracting under this chapter. [Section 435.06(2)(b)] Rescreening ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histoville
To all of you out there who dont already know, Ive created a facbook page called Histoville. It's a great place to ask for help, meet friends and discuss any issues relating to our field. We have already almost 300 members. I hope you will come and join us and help grow our community page. I look forward to making new histo friends. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Florida Society of Dermatologic Surgeons
The Florida Society for Dermatologic Surgeons will be holding there 30th annual meeting at the Peabody Hotel in Orlando, Fl Dec 2-4, 2011. They are looking for venders or members who are intersted in setting up a booth. The physicians interests are in: Pharmaceutical. Equipment. Pathology labs. Mohs consultation.Cosmetics. Please contact me and I can fax you the required documentation. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene
Might not be toxic but the fumes of citrus give me headaches and the smell is terrible.. Im a slide bright girl, no odor, and non flammable Hello Histonetters I have been reading about xylene, how dangerous it is. Have you thought about using mounting medium made from Limonene. Here is some info: *AR-6504 Organo (Limonene) mountâ¢:** *This mounting medium is made with limonene a natural product from orange peels. It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in Immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. This Organo mounting medium is also suitable with alkaline phosphatase chromogens, an organic solvent resistant Super Fast Red (IBSC, cat AR-8211). It is an excellent choice for mounting H and E stained slides. Coverslip is required. *DIRECT SUBSTITUTE OF Biomedaâs Clarion* This mounting medium is made by ImmunoBioScience corp. www.ImmunoBioScience.Com Have a nice day/weekend Mit freundlichen GrüÃen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種ã¨ã«ã¤ã㦠Bader Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: bade...@gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene alternative
Summary Explanation: SLIDE BRITE is a revolutionary dewaxing and clearing reagent for histologic techniques. It is a safe and effective alternative to xylene. It contains no carcinogens, no toxins, is odorless, and is classified as non-flammable and non-hazardous. Its flash point is above 140ºF (almost double of that of xylene). A qualified laboratory that is certified by the State of California Health Services has performed toxicity screening. SLIDE BRITE was designated non-hazardous on the basis of aquatic toxicity; thus eliminating hazardous waste and exposure to women of child-bearing age to xylene and cancer-causing reagents. It has non hazad shipping. No orange smell. does not have to be disposed of with hazardious chemicals. non-toxic. does not need to be stored in fire caninet. The only draw back I can find is it becomes saturated alot easier than xylene. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene alternative
Amber, I have a dermpath/ mohs lab and I use slide bright for everything. I have used the product for 9yrs now, at least. Slide bright is in my processor, H%E stain line and my deparrafin stain line. Definitly call and get sample. I love it. I get mine from Mercedes medical, belair(advantik), PSS, statlab, ect. I think 4gallons cost more than xylene, but save on shipping, disposal, and my overall health, so for me its a no brainer. The only strong odor I have is when I coverslip. PS does not counteract with acid or bluing. Works with all chemicals Good luck, Nicole Tatum HT, aSCP Who sells Slide Brite? Is that a cardinal product? Can you use it with regular alcohol, bluing and Acid alcohol on the HE stainer? That sounds like a great product to try in subbing for the xylene. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Wednesday, September 28, 2011 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene alternative Summary Explanation: SLIDE BRITE is a revolutionary dewaxing and clearing reagent for histologic techniques. It is a safe and effective alternative to xylene. It contains no carcinogens, no toxins, is odorless, and is classified as non-flammable and non-hazardous. Its flash point is above 140ºF (almost double of that of xylene). A qualified laboratory that is certified by the State of California Health Services has performed toxicity screening. SLIDE BRITE was designated non-hazardous on the basis of aquatic toxicity; thus eliminating hazardous waste and exposure to women of child-bearing age to xylene and cancer-causing reagents. It has non hazad shipping. No orange smell. does not have to be disposed of with hazardious chemicals. non-toxic. does not need to be stored in fire caninet. The only draw back I can find is it becomes saturated alot easier than xylene. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene alternative
Toni, Slide bright is everywhere xylene would have been. Proceesor, stainline, etc. I use either TBS shur/mount( xylene based) or S mounting media (toulene based). I think the xylene based media works better. As per the directions it can be flushed down the drain with copious amounts of water. But always check with your local enviromental agency. Nicole Tatum, HT ASCP Do you use this product for processing and staining? Do you need to use a particular type of mounting medium? How is it disposed of? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Wednesday, September 28, 2011 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene alternative Summary Explanation: SLIDE BRITE is a revolutionary dewaxing and clearing reagent for histologic techniques. It is a safe and effective alternative to xylene. It contains no carcinogens, no toxins, is odorless, and is classified as non-flammable and non-hazardous. Its flash point is above 140ºF (almost double of that of xylene). A qualified laboratory that is certified by the State of California Health Services has performed toxicity screening. SLIDE BRITE was designated non-hazardous on the basis of aquatic toxicity; thus eliminating hazardous waste and exposure to women of child-bearing age to xylene and cancer-causing reagents. It has non hazad shipping. No orange smell. does not have to be disposed of with hazardious chemicals. non-toxic. does not need to be stored in fire caninet. The only draw back I can find is it becomes saturated alot easier than xylene. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Facebook
Hello Histonetters, Just drooping in to say thanks to all those Who have joined Histo-ville on facebook. We are forming an awesome community and hope more will join. Stop by and like us. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Facebook
https://www.facebook.com/#!/pages/Histo-Ville/114622425272649 Here is the link for those of you who need it. Hello Histonetters, Just drooping in to say thanks to all those Who have joined Histo-ville on facebook. We are forming an awesome community and hope more will join. Stop by and like us. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] VIP PROGRAMMING
Kristy, 00 is today friday 01 tomorrow sat 02 the day after tommorrowsun 03 three days from today mon 04 four days from todaY TUES so for a regular weekend you would want the end time to be on day 03 at what ever time you set. this will end monday morning For a three days weekend or holiday you would want your end time to be 04 at what ever time you set. this will end tuesday morning. Hope this helps, Nicole Tatum, HT ASCP HI, EVERYONE QUESTION: WITH THE VIP SAKURA WHEN PROGRAMMING FOR A WKEND IS THE END DAY/TIME 3/00:00 OR 2/00:00 AND THEN FOR A 3 DAY WKEND (LIKE MEMORIAL DAY) IS THE END DAY/TIME 4/00:00 OR 3/00:00. JUST WANT TO MAKE SURE. THANK YOU FOR ALL THE HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Glass Slides
I put mine in the sharps box, unless I have alot then I put in cardboard hazard box. Nicole Tatum, HT ASCP I have a lot of slides that I have precut for stains. These slides are only labeled with the accession numbers and were not used as any part of the patient diagnosis. I was wondering how other labs were disposing of these slides. Thanks, Travis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathlogix
To those of you utilizing this software. Or if I have already spoken with you about it. Please contact me throught me personal email. I have figured it out!!! Nicole Tatum nic...@dlcjax.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] embedding centers
Stephanie, I prefur the Sakura Tissue-Teks. III being my fav, but it is becomming out dated and hard to find parts for. V is good to. The Leica brand is always a winner. Lots of people have these, so there easy to get parts and have maintenced. Make sure you check and see how many cassettes the warming tray can hold. If you processor holds 200, and your embedding cneter only hold 60 where do you put the rest of the blocks. Make sure the warming/holding area is large enough to hold all ur blocks. Good luck. If you buy refurbished you can save lots of money and with some companies still get a one year warrenty. Nicole.. Hello histonetters! I know this has been asked before, but there's not much in the recent archives. I'd like to see what everyone thinks is now the best paraffin embedding center. They all seem very similar now, and I don't see any one instrument that looks much different from the others. My primary concern is reliability and long working life, but of course I would also like an instrument that is user friendly, ergonomic and affordable. Please let me know if you have a very good experience with any embedding center or especially if anyone has had a particularly bad experience and let me know any features that you find spectacular or useless. Thanks for the advice! Stephanie Weaver Texas Veterinary Medical Diagnostic Laboratory ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Facebook
If you havent already come by and liked us, COME CHECK OUT HISTO-VILLE on facebook. We have over 100 member so far and hoping to expand. Come by and say hi. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Facebook
Under medical community How is this site listed? I searched for Histo-Ville, but nothing came up. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, September 13, 2011 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Facebook If you havent already come by and liked us, COME CHECK OUT HISTO-VILLE on facebook. We have over 100 member so far and hoping to expand. Come by and say hi. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo-ville
Thank you for joining the facebook group Histo-ville. I have already meet some great people. I hope more of you will join and use the facebook site as a way to communicate, make friends, and troubleshoot. I hope you meet you soon, Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Facebook
Hey histonetters, Ive created a community page for histologist on Facebook. Please join or like Histo-ville. It a page for people to make friends, troubleshoot, or discuss issues. I hope to create a large community of friends. So stop by and say hi. I look forward to meeting fellow techs. Nicole Tatum, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathlogix software
Hello Histos, Im looking for a women I spoke with in reguards to Pathlogix software. She helped me navigate the system..I need you please contact me ASAP. Nicole Tatum 904-400-5902 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening KC, Missouri
Busy Derm practice looking for an independant, hard working histotech, for a dermatopathology lab. Must be proficient in all aspect of routine histology. Grossing, staining, accessioning, mircotonomy. Full time position. Please call Tammi for immediate consideration. (816)584-8100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing of derm specimens
How many 100% do you have. I have 3. This is where the dehydration comes in. The 100 takes all the mositure out of the specimen. So this step is critical. Water and xylene are not soluable so if the specimens are not getting dehydrated properly, the xylene will not penertate the specimens either. Next is make sure your specimen are not thicker then 3mm. Espceially lipomas and cyst. Try to cut them as thin as possible. Note, the caseous inside of a cyst will likely not process and usually expoldes on a water bath. Next, make sure the specimen cassettes are propely placed inside the tissue rack. If flow between cassettes is restricted, a portion of a specimen could be raw. Hope this helps. Nicole Tatum HT ASCP I have recently had a problem with my skin specimens being underprocessed. I use a Leica 300ASP. The schedule is as follows: 10% NBF x 2 for 1 hour ea. 80% Reagent Alcohol for 1 hour 95% Reagent Alcohol x 2 for 1 hour each 100% Reagent Alcohol for 1 hour each Xylene x 3 for 1 hour each Paraffin x 3 for 1 hour each The specimens are mushy and swell on the ice Any input is welcome. send response to : _scrochiere@nedlc.com_ (mailto:scrochi...@nedlc.com) thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cell blocks
you definitly can run them together. I would just test one before I put them all in there, because when they are small they can over process and be very hard and brittle making intrepretation difficult. As far as your larger fatty gooey specimens. Make sure they are no thicker than 3mm. Anything bigger doesnt process well on a normal 7hr cycle. Hope this helps Nicole Tatum HT ASCP How does everyone process their cell blocks? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process. Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time. I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y. What does everyone else do? We use standard 10% NBF, xylene, and paraplast xtra on our processors. We would process on a Leica Peloris. Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opening in KC, Missouri
Full time or part time opening in Kansas City, Missouri for a busy Dermatopathology lab. Must be HT or HTL certified. Proficient in all aspects of routine histology. Grossing, coversliping, accessioning, microtonomy, and HE stains. Please call Tami at 816.584.8100 for immediate consideration. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] MOHS daily flow...
Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu mailto:bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Service Technician
Amanda, Im not exactly sure which part of your machine is freezing based on your description, but, I have a leica 1510 and it has freezing issues. Everyone I have ever used does this. The bar that hold the specimen chucks ices and freezes over like crazy.. It will frezze the chuck in the bar holder solid. I have to get a hammer and beat it sometimes..lol. So, before I use my machine I wipe 100% alcohol across the bar to de-ice it and the chucks dont get stuck..Do not use so much that you lower your temp. Also, do not get on the oct or the stage because your blocks will not cut and be mush... Just wipe the areas daily with alcohol.. Hope this helps. Nicole Tatum, HT ASCP Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Could you please recommend? (Bascaramurty, Saro)
Not sure about the UV thingy but I love the Leica 1850 or the 1510 works great. But, if I had to choose between the two I would go with the Leica 1850.. You can purchased a used cryo for about 12-15,000. Most times with a one year warrenty. I recommend calling Margaret at IMEB for a quote. She is wonderful and will find what you need. Nicole Tatum HT(ASCP) I second the Leica! :o) Michelle Sent from my iPhone On Jun 29, 2011, at 1:14 PM, White, Lisa M. lisa.whi...@va.gov wrote: We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz, Leica and a unit so old I think it came with Noah on the boat don't even remember the maker. Leica is the favorite. It sections well and was the first one that the anti-roll plate would work correctly. It decontaminates well. No regrets, love it and would recommend. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome blade donations
Do they take volunteer histos, :) I would love to do somethig like that. Nicole Hi, One of our pathologists is going on a volunteer mission to a pathology lab in Malawi, Africa in a few weeks, and he is looking for donations of low profile microtome blades for the histology lab there. He said they will need around 200 blades or so, but any amount would help. He leaves on July 21st, so we are under a bit of a time crunch. Thanks! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen tissue question
I run a Mohs lab that processes skin by frozen section. There is no need to use any fixative before hand. But, if you are looking for melanoma or melanocytes, freezing can cause artifact and make it difficult to read slides. Limit the amount of nitrogen you use to freeze the specimen. Some places use alcohol as a fixative after the slide is cut. It will for all purposes remove the water continent from the tissue which will preserve tissue. Hope this helps. Nicole Tatum HT ASCP We have a new doctor in our lab who swears that all frozen tissue must be fixed in formalin with a subsequent sucrose treatment before freezing in OCT because not fixing it will cause the structures to be distorted and you can't get good antibody attachment. In my previous experience, we have done this with tissue that came from an animal that was perfused with formalin before the tissue was removed. However, the majority of my previous frozen specimens were flash frozen in OCT and fixed after sectioning. It is also my understanding that fixation in formalin can cause poor antibody detection because of cross-linking caused by the formalin. I would like to hear some other opinions on this please. The particular specimen we will be working with is skin. Thanks in advance, Joel Reichensperger -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensper...@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Keeping Histo room floor clean?
I have a tool that I got from those odd ball lab supply company that carry a little of everything. Its long like a broom handle and has a scraper blade on the bottom with a removable replaceable blade. Cost like 20 bucks works great then I just sweep it up. Nicole Tatum, HT ASCP You can buy this type of thing too if you aren't the McGiver type. For instance...from American Mastertech item # CPW04200E Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Tuesday, June 14, 2011 7:50 PM To: JR R Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Keeping Histo room floor clean? We don't keep it off the floor, but do use a wide-bladed putty knife attached to a mop handle to scrape the residual wax off the floor. It woks quite nicely and doesn't remove the actual floor wax like a razor blade scrapper would. Michelle Sent from my iPhone On Jun 14, 2011, at 6:44 PM, JR R rosenfeld...@hotmail.com wrote: We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station. Short of laying down a tarp, what do folks do keep wax off of the floor? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AP software
Hello Histonetters, I need help. So I have used Pathlogix software for about 4yrs and I love it. It so super simple and have never had any problems. Well, except for one. The cost of leasing has increase by 19% each year for no apprearent reason. We are now paying quarterly triple what we leased it for 4yrs ago. The owner says cost are riseing due to the economy and prices are subject to change without notice. Really! 19%. Cause im pretty sure thats bad business and thats how you lose customer instead of keeping them. At any rate, I need to find a new company. I have looked into a few systems but the cost are hugh. We are a small derm lab with one reading physician and im the only tech. So I do not need a large system. I need to be able to generate path reports. Accession, create reports and logs for about 3000 specimen a year. I wish I could just purchase outright but will lease if we have to. I havent had must luck at finding software for small labs except Pathlogix. Love the software, not so fond of the company. Please tell me what software is out there and If you guys like it. Thank a million, Nicole Tatum HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Does a Path lab for any reason need a disposal
Does anyone know, for any reason why a dermatopathology lab would be or is required to have a garbage disposal? Sound like a stupid question, but wanted to make sure... Thanks fellow Histo's Nicole Tatum HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Two Questions
1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on HE slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biopsy Run
I use the sponges and I keep them in a water so the formalin can penetrate when placed in the processor. Make sure when you put the sponges in the water that you mash them to get out the air bubbles. I use water just in cause my little bowl spills. If it was in formalin I would have to break down my whole grossing station and turn on hood. You can also use kim wipes. Just cut you some squares and place tissue in middle then fold and put in cassette. Hope this helps. Nicole And one more thing Soak the sponges in formalin... j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yahoo Sent: Wednesday, April 27, 2011 08:56 To: Marshall, Kimberly K Cc: Histonet Subject: Re: [Histonet] Biopsy Run Mesh cassettes are known to hold air pockets in them, so extending your times would only over process your tissue. I would suggest you use sponges, filter paper, or bx bags and use the cassettes you use for your larger specimens until you can get a different cassette. We found in our lab (we didn't have a printer) that the ink will fade if it's not dry enough before blocks are put in formalin container. Same on the slides. Hope this helps. Sent from the iPhone of Kim Tournear On Apr 27, 2011, at 7:21 AM, Marshall, Kimberly K kkmarsh...@anthc.org wrote: Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil nuclear artifact. We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problem with IMEB
I recently used IMEB for the first time and have had a wonderful experience. My rep Margaret has gone above and beyond to satisfy all my questions and needs. She was always very deligant in getting answers to my questions. The poor women probably had to do four or five quotes before we finally hashed it all out. I have used Belair and Pacific in the past. But, I would give my order to Margaret anytime. Nicole Tatum HT(ASCP) Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] clia reg on ventilation
Can anyone help me find some kind of standard for ventilation in a pathology laboratory. I cant seem to find anything more then adequate ventilation. Ok well what definies adequate? Is there a set Clia standard or AHCA standard for ventilation in Flordia. Thank you for your help. Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] link to OSHA reg for lab ventilation
http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=standardsp_id=10106 link to lab standard including ventilation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica autostainer XL
Does anyone know if you can just deparaffinize slides in the stainer. Im just wondering if I also need a small stain line to dewax specials. Thank you, Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
