Re: [Histonet] Histonet Digest, Vol 234, Issue 10

[Patsy Ruegg via Histonet]

Aren't they going to offend the mail histotechs with that name? Just kidding.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Wednesday, May 10, 2023 11:00 AM
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Subject: Histonet Digest, Vol 234, Issue 10

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Re: [Histonet] Histonet Digest, Vol 233, Issue 7

[Patsy Ruegg via Histonet]

this is a strange post on histonet

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Wednesday, April 19, 2023 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 233, Issue 7

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Re: [Histonet] Histonet Digest, Vol 223, Issue 14

[Patsy Ruegg via Histonet]

I used tonsil as + control.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, June 24, 2022 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 223, Issue 14

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Re: [Histonet] Histonet Digest, Vol 213, Issue 22

[Patsy Ruegg via Histonet]

I would suggest using something like histogel or agar to make layers of blood 
drops in a tube, then freeze them and make frozen sections. Sounds tricky.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Tuesday, August 31, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 213, Issue 22

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Re: [Histonet] Histonet Digest, Vol 211, Issue 7

[Patsy Ruegg via Histonet]

The blue stain from prussian blue is a chemical reaction and indicates that 
there is a lot of iron in the section, you would not want it not to stain some 
of the iron there would you? I agree with John, take an adjacent section for a 
general stain.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Monday, June 7, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 211, Issue 7

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Re: [Histonet] Water under sections

[Patsy Ruegg via Histonet]

This is all good advise. I always picked up section and tapped the slide on 
counter and then dried standing up. This is really a problem if you are at all 
above 5K feet in altitude where water boils violently at 92-93dc, which will 
destroy your tissue section. I have written a couple of papers about on this.


From: Greg Dobbin 
Sent: Friday, January 22, 2021 11:45 AM
To: b-freder...@northwestern.edu 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Water under sections

Hi Bernice,
In my lab, water under the sections is unique to charged slides. And you
are correct, if there is water under the section when the slides are heated
for antigen retrieval, the boiling (or at least very hot) water will damage
or entirely destroy the section.

We allow the charged slides to drain (upright) for a few minutes and then
carefully make a hole in the edge of the wax and use a Kimwipe or paper
towel to carefully wick the excess water out as much as we can without
touching the tissue section. Then we "flick" or shake sharply to remove any
residual water that may remain before we bake them (FYI, we choose not to
bake on the stainer). If after baking 30 mins at 60C, we notice water
(maybe someone was not as diligent earlier?), we wick away the excess and
bake for another 15 mins to ensure good adhesion of the section to the
slide before proceeding to the immunostainer. Hope this helps.
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*

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Re: [Histonet] Microtome at home

[Patsy Ruegg via Histonet]

I have done it, but you are right, I had my own private business, not sure why 
it would be a problem, especially for research.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jamie Watson 
Sent: Wednesday, April 15, 2020 5:44 PM
To: Histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Microtome at home

Hello all,

Our pathologist has come up with the idea of sending a microtome and waterbath 
home to someone that cannot come to work due to COVID 19.  We are a research 
lab and work with mouse and rat tissue.  Does anyone know of any issues with 
doing this?  I have never heard of anyone cutting slides at home other than 
someone with a private business.

Thank you.

Jamie

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Re: [Histonet] FW: Microtome at home

[Patsy Ruegg via Histonet]

I agree with this point and as far as clocking in and out, I would think you 
could work out something like getting paid piece mill, perhaps charge per slide 
or block cut, that way you could do it on your own time and not have to clock 
in.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Joseph Saby 
Sent: Thursday, April 16, 2020 8:03 AM
To: Porter, Amy ; Porter, Amy via Histonet 
; histonet@lists.utsouthwestern.edu 
; Steven Crochiere 
Subject: Re: [Histonet] FW: Microtome at home


You will need to make sure all pertinent SOPs and EOPs are followed, as well as 
all safety guidelines/protocols. Just because it is not human tissue doesn't 
mean that it can't have its share of nasties.
Joe Saby

Sent from Yahoo Mail on Android

  On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via 
Histonet wrote:   Make sure of insurance 
coverage and safety for the employee and that they are covered in case of 
injury - are they still clocking in and out in some fashion. just thinking 
in a bigger box.


From: Steven Crochiere via Histonet 
Sent: Thursday, April 16, 2020 6:36 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] FW: Microtome at home

Jaime,

I don't see a problem with a research setting. If it was patient care, CLIA 
would need to inspect the set up in the person home. The same goes for our 
pathologists who read slide at home.

Steve

-Original Message-
From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 15, 2020 7:51 PM
To: Jamie Watson ; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome at home

I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from 
my Verizon, Samsung Galaxy smartphone
 Original message From: Jamie Watson via Histonet 
 Date: 4/15/20  6:44 PM  (GMT-06:00) To: 
Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello 
all,Our pathologist has come up with the idea of sending a microtome and 
waterbath home to someone that cannot come to work due to COVID 19.  We are a 
research lab and work with mouse and rat tissue.  Does anyone know of any 
issues with doing this?  I have never heard of anyone cutting slides at home 
other than someone with a private business.Thank 
you.Jamie___Histonet mailing 
listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Re: [Histonet] Need a procedure

[Patsy Ruegg via Histonet]

From: John Garratt 
Sent: Thursday, January 23, 2020 10:51 AM
To: Terri Braud 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Need a procedure

Hi Terri, I suggest you use Histogel for block preparation. It works 
exceptionally well, it is good for IHC and does not have the pitfalls of 
plasma/thrombin.
Plasma/thrombin does work well for cell blocks but you will have to consider an 
ethical and safe source for your plasma.
The instructions for using Histogel are in the package insert though I have one 
comment. Be careful how you warm the Histogel and use a heat block. Do NOT use 
a microwave since there is a tendency to overheat the gel and you will end up 
with poor quality IHC.

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet 
 wrote:

> Hi fellow Histonetters - I'm in need of some help, please
> Background - We currently use agar to capture our scant cell blocks for 
> processing. I am unfamiliar with the Plasma/Thrombin method of cell block 
> preparation and am interested in comparing it to our current method
> Request - Could you please send me your procedures for this method, 
> specifically where you purchase your plasma and thrombin and what species are 
> used?
> Thanks in advance. Histotechs rock!
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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Re: [Histonet] Histonet Digest, Vol 195, Issue 10

[Patsy Ruegg via Histonet]

I never minded being called in to assist for an aspect of transplants at the U, 
I figured I was helping a very needy patient.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Thursday, February 13, 2020 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 195, Issue 10

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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Re: [Histonet] Need a procedure

[Patsy Ruegg via Histonet]

Terri, when I was in animal research we used the blood from the animal to make 
plasma and combined it with some thrombin from the hospital pharmacy.  Of 
course that is tedious especially since histogel is available.  I warmed the 
histogel by placing a tube of it in a beaker of hot water, do not mw it.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: John Garratt 
Sent: Thursday, January 23, 2020 10:51 AM
To: Terri Braud 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Need a procedure

Hi Terri, I suggest you use Histogel for block preparation. It works 
exceptionally well, it is good for IHC and does not have the pitfalls of 
plasma/thrombin.
Plasma/thrombin does work well for cell blocks but you will have to consider an 
ethical and safe source for your plasma.
The instructions for using Histogel are in the package insert though I have one 
comment. Be careful how you warm the Histogel and use a heat block. Do NOT use 
a microwave since there is a tendency to overheat the gel and you will end up 
with poor quality IHC.

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet 
 wrote:

> Hi fellow Histonetters - I'm in need of some help, please
> Background - We currently use agar to capture our scant cell blocks for 
> processing. I am unfamiliar with the Plasma/Thrombin method of cell block 
> preparation and am interested in comparing it to our current method
> Request - Could you please send me your procedures for this method, 
> specifically where you purchase your plasma and thrombin and what species are 
> used?
> Thanks in advance. Histotechs rock!
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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Re: [Histonet] Frozen Gel Pack with paraffin block for shipping?

[Patsy Ruegg via Histonet]

whether I used an ice pack or not, I always bagged each block in it's own 
individual container/plastic bag, etc., just in case there was melting the 
tissue could possibly be recovered.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Paula 
Sent: Wednesday, March 6, 2019 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Gel Pack with paraffin block for shipping?

Hello,



This question has come up here at work a few times. Apparently, the
department does different protocols, depending on who is doing the job of
shipping out a paraffin block for additional testing. Our transcription
department handles this and I was asked to look into it so I can submit a
protocol for them to follow.



Is there an industry standard to follow?  I'm leaning towards always putting
in an frozen gel pack during the warmer months inside the shipping container
to avoid any melted blocks. I know the melting point is somewhere around
136F (58C), but I still want to ensure the block doesn't get warped or
altered in any way because of the heat. Plus, we shouldn't have to check the
weather before sending out and I think it  just should be our standard
protocol.



What are your thoughts and if there is a standard, please share that with
me.



Thank you very much,

Paula

Lab Manager


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Re: [Histonet] Histonet Digest, Vol 184, Issue 1

[Patsy Ruegg via Histonet]

here here

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Joseph Saby 
Sent: Tuesday, March 5, 2019 9:47 AM
To: Jordan, Kelley; Mark Tarango
Cc: HistoNet
Subject: Re: [Histonet] Histonet Digest, Vol 184, Issue 1

The more important question is who the h*ll is Kelly Jordan.
Terri has established her competency and reputation in NSH and the Histonet for 
probably over 20 years.
You have done your company a great disservice.
People will remember you, probably not as you would wish.
Joe Saby, retired


On Tuesday, March 5, 2019, 11:42:30 AM EST, Mark Tarango via Histonet 
 wrote:

 How about passing on to the department that could fix the issue?  Where's
the empowering innovation?

On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> More bad press in histonet...not sure if we should pass it on to
> Marketing??
>
> @Will, Who as Teri Blaud at  Holy Redeemer Hospital ? Teri is always
> always bashing us.
> Kelley Jordan
>
> Strategic Account Manager - SC, NC, TN and KY
>
>
> A Member of the Roche Group
>
> Ventana Medical Systems
>
> Mobile:  803.504.1135
> Customer/Technical Support: 1.800.227.2155
>
> kelley.jor...@roche.com
>
> www.ventana.com
>
>
>
> Empowering | Innovation
>
>
> Confidentiality Note: This message is intended only for the use of the
> named recipient(s) and may contain confidential and/or proprietary
> information. If you are not the intended recipient, please contact the
> sender and delete this message. Any unauthorized use of the information
> contained in this message is prohibited.
>
> histonet 
>
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Re: [Histonet] Histonet Digest, Vol 184, Issue 1

[Patsy Ruegg via Histonet]

Maybe that is the trouble, they keep passing these technical issues on to 
Marketing instead of R

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com

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Re: [Histonet] New York Qualifications for Histology Supervisor

[Patsy Ruegg via Histonet]

In my opinion this issue is related to the fact that HT's are not required to 
have BS degrees, like Med Techs and Cytotechs, and that clia/cap does not 
require ASCP certification to work in a histology lab.  We have always been the 
red headed step child in the lab because of this.  This is why NSH has fought 
so hard to at least require the AS degree with certain science credits.  As far 
as I know, unless it has changed CLIA doesn't even require that those working 
in histology be ASCP certified.  I may be behind on that rule???  I know many 
very qualified folks who are stars in histology who do not have a BS degree, 
but I think those times are behind us, as sheep skin seems to count for more 
than experience in this new world.  It is a shame because that sheep skin does 
not a good Histotech make, on it's own.

Patsy


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Perl , Alison 
Sent: Thursday, February 21, 2019 10:46 AM
To: 'Clare Thornton'; 'Kelli Goodkowsky'
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] New York Qualifications for Histology Supervisor

Very exciting to hear NSH is working on it with CLIA! I hope the NYS 
legislature/OOP will take cues from them

And I just emailed my rep :)

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
(914) 302-8424
ap...@cmmedical.com


-Original Message-
From: Clare Thornton [mailto:cthorn...@dahlchase.com]
Sent: Thursday, February 21, 2019 12:39 PM
To: 'Kelli Goodkowsky'; Perl , Alison
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] RE: [Histonet] New York Qualifications for Histology 
Supervisor

NSH is currently working directly with CLIA to addresss issues such as this.  
Stay tuned!


Clare J. Thornton, HTL(ASCP)CM, QIHCCM
Lead Immunohistochemistry Tech
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com




-Original Message-
From: Kelli Goodkowsky via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 21, 2019 12:11 PM
To: Perl , Alison 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] New York Qualifications for Histology Supervisor

This is good information, Alison.  Thank you for sharing it.  Our Histology 
Program is in CT and I have many students from the southern part of the state, 
making NY licensing a viable option for them. It would be interesting to note 
how NY is defining “judgment” when it comes to the role of 
histotechnicians/histotechnologists.  I sit on the Educator’s Task force for 
the NSH and will bring this to their attention as well.


Kelli Goodkowsky, M.Ed., HT (ASCP)
Program Director, Histologic Science
President, Faculty Senate
Goodwin College
(860) 727-6917
kgoodkow...@goodwin.edu
http://www.goodwin.edu










On Feb 21, 2019, at 11:51 AM, Perl , Alison via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:

Hi Melissa
This is true, and a source of my neverending frustration with NYS Office of 
Professions. Stephanie Shulman 
(stephanie.shul...@health.ny.gov) is a 
good person at NYS to get clarification, but she will reiterate the same.

NYS has explained to me that "histotechs don't exercise judgment" and thus are 
not qualified to take on the role of supervisor, even for a histology-only 
laboratory. The supervisors here in my lab and myself all have Clinical Lab 
Technologist licenses, from when they were grandfathered back in 2007. I have 
techs who went to school specifically for Histology, but can never become 
supervisors. I don't know what anyone will do for the next generation of 
supervisors - your situation is a real fear.

Anyone in NYS who would like to raise this issue with your representatives, I 
encourage you to do so!!

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
(914) 302-8424
ap...@cmmedical.com


-Original Message-
From: Melissa Owens via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 21, 2019 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor

Hello,

I have a question about the requirements to be a supervisor in New York. New 
York dept. of Ed states qualifications for a Medical Technologist/Laboratory 
Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it 
mention qualifications for a Histototech/Histology Supervisor. So therefore, I 
have heard that in New York, a Histology Supervisor must qualify under the 
Medical Technologist/Laboratory Supervisor qualifications statues. This seems 
impossible to me as this would have to be an individual who becomes a Medical 
Technologist and by random transfers into a histology role then becomes a 
supervisor. Does anyone out 

Re: [Histonet] Bone PMMA sections

[Patsy Ruegg via Histonet]

Stefano, after cutting the sections, dry them on a heat plate, then they can be 
stored dried stacked next to each other.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: MANTERO Stefano RIC 
Sent: Thursday, February 21, 2019 7:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone PMMA sections

Good morning Histonetters!

I'm starting to cut out of bone samples included in PMMA.
I would like some suggestion how to storage the slides cut.

Many thanks in advance

Stefano

Dott. Stefano Mantero

Human Genome Laboratory  CNR-IRGB
c/o Humanitas Research Hospital

Via Rita Levi Montalcini (Ex Via Dainese)
20090 Pieve Emanuele (MI)

Ph. +39 02 8224 5164 (desk)
Ph. +39 02 8224 5177 (lab)
Fax +39 02 8224 5191



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persone diverse dal destinatario è severamente proibito, ed è pregato di 
rinviarlo immediatamente al mittente distruggendone l'originale.
Grazie
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Re: [Histonet] Re guinea pig IHC

[Patsy Ruegg via Histonet]

good advise Carl and you asked some of the things I was wondering.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Hobbs, Carl 
Sent: Saturday, February 2, 2019 12:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re guinea pig IHC



Ms Ruegg, as usual , gives excellent advice: avoid HRP.
Use Alk phos?
Block end. alk phos by using levamisole.

However, Ms Shivers does not state the fixation status of her FS.
Is the Gpig tissue perfused-fixed then frozen orfrozen as unfixed...then 
fixed?
Alsowhy 0.3% H2O2?
Use 3%kill the enzyme, not feed it?
NOT aq for FS
Make up in IMS ( 74OP)
No tissue disruption
However: you state that you get loadsa bubbles...so what? Is your section still 
attached to your slide?
Can you then carry out successful IF/IHC?
If yesno problem.
Sure, there's the argument that using a coagulant ( alcohol) in block is a No No
I never had a problemprovided that  the Formalin fixation was sufficient 
for unfixed crosections ( 15 mins)
I do STILL severely dislike FS ( sure, I spent many years in Diagnostic 
Histopath doing Operative FS)
 Why not use Pwax sections, Ms Shivers?

Curious-illy

Carl



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813



From: histonet-requ...@lists.utsouthwestern.edu 

Sent: 02 February 2019 18:00
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 183, Issue 2


Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
 
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Today's Topics:

   1. Re: cytology listserv (Webster, Thomas S.)
   2. Re: guinea pig IHC (Patsy Ruegg)


--

Message: 1
Date: Fri, 1 Feb 2019 18:28:30 +
From: "Webster, Thomas S." 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] cytology listserv
Message-ID: <93fc6a1cc62f41f5ad5890786ab04...@crh.org>
Content-Type: text/plain; charset="us-ascii"

Haven't seen any cytology listservs except the one for members of the ASC.  
There are some cytology facebook pages where you could get questions answered. 
This Histonet listserv is very informative.


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Columbus Regional Hospital
2400 East 17th Street
Columbus, Indiana 47201

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Message: 2
Date: Fri, 1 Feb 2019 18:39:54 +
From: Patsy Ruegg 
To: Jan Shivers , histonet

Subject: Re: [Histonet] guinea pig IHC
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Especially a very blood tissue like GP spleen.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Patsy Ruegg 
Sent: Thursday, January 31, 2019 11:51 AM
To: Jan Shivers; histonet
Subject: Re: [Histonet] guinea pig IHC

In my experience it is not that GP have a higher peroxidase level, it is frozen 
sections in general that cannot be blocked with h202, unless they are fixed for 
a long time in formalin.  What are others experiences with h202 blocking on 
frozen sections.  I always  used an IHC detection system that did not require 
h202 blocking for frozen sections.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jan Shivers 
Sent: Tuesday, January 29, 2019 12:58 PM
To: histonet
Subject: [Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And 

Re: [Histonet] guinea pig IHC

[Patsy Ruegg via Histonet]

Especially a very blood tissue like GP spleen.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Patsy Ruegg 
Sent: Thursday, January 31, 2019 11:51 AM
To: Jan Shivers; histonet
Subject: Re: [Histonet] guinea pig IHC

In my experience it is not that GP have a higher peroxidase level, it is frozen 
sections in general that cannot be blocked with h202, unless they are fixed for 
a long time in formalin.  What are others experiences with h202 blocking on 
frozen sections.  I always used an IHC detection system that did not require 
h202 blocking for frozen sections.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jan Shivers 
Sent: Tuesday, January 29, 2019 12:58 PM
To: histonet
Subject: [Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like continuous champagne bubbles
rising out of the tissue, even after 20 minutes in the H2O2 solution.

I can't find anything in the literature that mentions guinea pigs having a
higher peroxidase content in their tissues.

Thanks for any help that anyone can provide.

Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

*Confidentiality Notice: This message, together with any attachments, is
intended only for the use of the individual or entity to which it is
addressed and may contain confidential or privileged information. If you
think you have received this message in error, please advise the sender and
then delete this message and any attachments immediately.*


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Re: [Histonet] guinea pig IHC

[Patsy Ruegg via Histonet]

In my experience it is not that GP have a higher peroxidase level, it is frozen 
sections in general that cannot be blocked with h202, unless they are fixed for 
a long time in formalin.  What are others experiences with h202 blocking on 
frozen sections.  I always used an IHC detection system that did not require 
h202 blocking for frozen sections.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jan Shivers 
Sent: Tuesday, January 29, 2019 12:58 PM
To: histonet
Subject: [Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like continuous champagne bubbles
rising out of the tissue, even after 20 minutes in the H2O2 solution.

I can't find anything in the literature that mentions guinea pigs having a
higher peroxidase content in their tissues.

Thanks for any help that anyone can provide.

Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

*Confidentiality Notice: This message, together with any attachments, is
intended only for the use of the individual or entity to which it is
addressed and may contain confidential or privileged information. If you
think you have received this message in error, please advise the sender and
then delete this message and any attachments immediately.*

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Re: [Histonet] IDH1 source

[Patsy Ruegg via Histonet]

Hi Richard,
How are you?  Dianova is a really good ab supplier, I used their CD31 for 
animal research and it was excellent.  Happy Holidays.  Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: John Garratt 
Sent: Sunday, December 16, 2018 2:37 PM
To: Anne van Binsbergen via Histonet
Subject: Re: [Histonet] IDH1 source

If you check out the assessments page at www.ciqc.ca you 
will see EQA runs for IDH1. The protocols in the run reports will show the 
source of the Ab.

John

Sent from ProtonMail Mobile

On Sat, Dec 15, 2018 at 5:15 PM, Anne van Binsbergen via Histonet 
 wrote:

> Good morning to all
> Richard Cartun: we use IDH1 on FFPE tissue.
> We purchase from Dianova, Germany. (I believe they are still the only 
> suppliers worldwide).
> Purchased direct via their website.
> Vendors will also supply from the same source but at a higher price.
> Good luck
> Merry Christmas everyone!
> Anne
>
> Anne S van Binsbergen
> Supervisor, Histology Laboratory
> Pathology and Laboratory Medicine
> Sheikh Khalifa Medical City
> Abu Dhabi
> United Arab Emirates
>
> Sent from my iPhone
>
>> On Dec 15, 2018, at 10:00 PM, histonet-requ...@lists.utsouthwestern.edu 
>> wrote:
>>
>> Send Histonet mailing list submissions to
>> histonet@lists.utsouthwestern.edu
>>
>> To subscribe or unsubscribe via the World Wide Web, visit
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>> histonet-requ...@lists.utsouthwestern.edu
>>
>> You can reach the person managing the list at
>> histonet-ow...@lists.utsouthwestern.edu
>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>> 1. IDH1 (Cartun, Richard)
>>
>>
>> --
>>
>> Message: 1
>> Date: Fri, 14 Dec 2018 20:38:38 +
>> From: "Cartun, Richard" 
>> To: "histonet@lists.utsouthwestern.edu"
>> 
>> Subject: [Histonet] IDH1
>> Message-ID:
>> <9215bd4b0ba1b44d962a71c758b68d2eac0e2...@hhcexchmb03.hhcsystem.org>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> For those labs that are performing IDH1 IHC testing on FFPE tissue, which 
>> clone are you using and where do you get it? Thank you.
>>
>> Happy Holidays to everyone.
>>
>> Richard
>>
>> Richard W. Cartun, MS, PhD
>> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
>> Proteomics Laboratory
>> Director, Biospecimen Collection Programs
>> Assistant Director, Anatomic Pathology
>> Hartford Hospital
>> 80 Seymour Street
>> Hartford, CT 06102
>> (860) 972-1596 (Office)
>> (860) 545-2204 (Fax)
>> richard.car...@hhchealth.org
>>
>>
>> This e-mail message, including any attachments, is for the sole use of the 
>> intended recipient(s) and may contain confidential and privileged 
>> information. Any unauthorized review, use, disclosure, or distribution is 
>> prohibited. If you are not the intended recipient, or an employee or agent 
>> responsible for delivering the message to the intended recipient, please 
>> contact the sender by reply e-mail and destroy all copies of the original 
>> message, including any attachments.
>>
>>
>> --
>>
>> Subject: Digest Footer
>>
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>> End of Histonet Digest, Vol 181, Issue 5
>> 
>
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[Histonet] Fw: Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL

[Patsy Ruegg via Histonet]

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com




From: ancillaryp...@mac.com 
Sent: Saturday, October 7, 2017 6:03 PM
To: ihcrg ihcrg
Subject: Announcing the 12th annual retreat of applied immunohistochemistry and 
molecular pathology at the beautiful Marriott, Key Largo, FL

Dear colleagues at IHC Resource Group,

Following pre-hurricane preparation and 2 weeks of lack of electricity 
post-hurricane, we’re finally able to get our lives back on track, hence this 
delayed announcement for the 12th annual AIMP retreat. Please forward this 
email to as many folks as you can. Those of you who attended previous retreats 
will receive a decent discount as specified on the website.

Please let me know if you have any questions. I hope to see you in the FL Keyes 
in February.

Best regards,

Hadi

Hadi Yaziji, MD, FCAP, FASCP
Medical Director
Vitro Molecular Laboratories
8700 W. Flagler Street, Suite 100  l   Miami, FL 33174
www.vitromolecular.com
Vitro Molecular Laboratories | Anatomic 
Pathology
www.vitromolecular.com
Vitro Molecular Laboratories provides routine and specialty anatomic pathology 
services to a loyal physician clientele.


hyaz...@vitromolecular.com
Office 305-267-7979  l   Fax 786-513-0175


[https://gallery.mailchimp.com/b4d3e9ff192b16fd68e3363a0/images/9e4a48fa-48d5-43fe-ac8c-f45b596f7767.jpg]




12th Annual International Retreat on Applied Immunohistochemistry & Molecular 
Pathology


Previous AIMP retreat attendees have attested that AIMP has been “the best 
pathology CME event ever attended”. Our 12th annual retreat will have the 
following list of speakers (arranged alphabetically):



Noah Brown, MD; University of Michigan

Richard Cartun, PhD; Hartford Hospital

Carol Cheung, MD, PhD., JD, FRCPC, University of Toronto

Brenda Cox, FHFMA, CPC, MT (ASCP)

Richard Eisen, MD; Banner Health

Joel Greenson, MD; University of Michigan

Jason Hornick, MD, PhD; Harvard University

Jeffrey Myers, MD; University of Michigan

Emina Terlacovich, MD, PhD; University of Saskatchewan

Megan Troxell, MD; Stanford University

Hadi Yaziji, MD, Vitro Molecular Laboratories



Location: Key Largo Marriott (Key Largo, FL, USA)

Date: February 4 - February 8, 2018


Topics at a glance:



Sunday, February 4

Welcome and Opening Remarks [Hadi Yaziji]

Pre-Test [Hadi Yaziji]

Interpreting FISH Assays by The Practicing Pathologist - General Principles  
[Hadi Yaziji]

Best of USCAP 2017: IHC Applications [Richard Eisen]

Developing Fit-For-Purpose IHC Assays [Carol Cheung]

Validation vs. Verification of IHC Assays:  The Clue is in the Test Performance 
Characteristics [Emina Torlakovic]

Tissue Tools are Power Tools for the IHC Laboratory.  [Carol Cheung]

Controlling the Controls in IHC – a Path to Standardization [Emina Torlakovic]

Monday, February 5:

Malignant Mesothelioma and Other Diffuse Pleural Tumors [Jeffrey Myers]

Role of the Pathologist in Diagnosis and Management of Patients with NSCLC 
[Jeffrey Myers]

Interpreting FISH Assays: Common Assays & Specific Scenarios  [Hadi Yaziji]

Molecular Genetics of Colon Cancer, what a practicing surgical pathologist 
needs to know [Joel Greenson]

Specimen Considerations for Solid Tumor Molecular Testing [Noah Brown]

PD-L1: Interpretation Pitfalls & Update on Utility as a Companion Diagnostic 
Assay [Hadi Yaziji]

Tuesday, February 6:

HER2 Testing: 2018 ASCO/CAP Guidelines - What’s New and Why [Hadi Yaziji]

Troubleshooting Diagnostic Immunohistochemistry [Rich Cartun]

Open Mic: Bring Your Own Questions - Part I  [Hadi Yaziji]

Best “Specialized” Markers (Myoepithelial, Cytokeratins, Neuroendocrine, 
Endothelial, Histocytic, Megakaryocitic, Erythroid, ETC) in IHC [Hadi Yaziji]

Surgical Pathology Case Presentations - Session 1 [Faculty]

Surgical Pathology Case Presentations - Session 2 [Faculty]

Wednesday February 7:

The Evolution of IHC for Soft Tissue Tumors in the 21st Century: From 
Differentiation to Molecular Genetics [Hornick]

Immunohistochemistry in Kidney Tumors: The New WHO Classification and Beyond 
[Megan Troxell]

Beyond Lineage: Diagnostic and Predictive Molecular IHC for Surgical 
Pathologists [Jason Hornick]

Immunohistochemistry in Transplant Pathology [Megan Troxell]

Surgical Pathology Case Presentations - Session 3 [Faculty]

Surgical Pathology Case Presentations - Session 4 [Faculty]



Thursday, February 8:

Essential Coding & Compliance: 2018 Update - Part I [Brenda Cox]

Essential Coding & Compliance: 2018 Update - Part II [Brenda Cox]

Open Mic: Bring Your Own Questions - Part II  [Hadi Yaziji]

Open Mic: Bring Your Own Questions - Part III  [Hadi Yaziji]

Surgical Pathology Case Presentations - Session 5 [Faculty]

Surgical Pathology Case Presentations - Session 6 [Faculty]


To 

Re: [Histonet] Survey!!!!!!

[Patsy Ruegg via Histonet]

I have seen so many more problems with auto coverslippers, they break down, the 
cover does not hold up over time for archiving, etc., that I would would 
probably chose an autostainer.



Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com




From: Ifeoluwa Ajayi 
Sent: Saturday, April 1, 2017 6:11 AM
To: Patti Nelson - PNP Lab Consultant
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Survey!!

Personally I will prefer auto coverslipper, because cover slipping is
cumbersome and takes time.

Ajayi Ifeoluwa, BMLS, AMLSCN, MSc,
UCH Ibadan, Nigeria.

On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi Everyone,
>
> I just wanted to get everyone's opinion. If you had to chose between
> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you
> chose? Lets say your volume was around 60 to 80 blocks a day and you worked
> for a GI Lab. Everyone's input would be greatly appreciated.
>
>
>
> Sincerely,
>
> PATTI NELSON  H.T.(ASCP)
> PNP LABORATORY CONSULTANTS
> SUPERVISOR DGC/ZADEH LABS
> PO BOX 412
> CABAZON, CA. 92230
> 909-841-9761
> nelsonr...@verizon.net
> CONFIDENTIALITY NOTICE:This message and any included attachments are from
> Patti Nelson, PNP Laboratory Consultants and are intended only for the
> addressee. The information contained in this message is confidential and
> may contain privileged, confidential, proprietary and/or exemption from
> disclosure under applicable law.  Unauthorized forwarding, printing,
> copying, distribution, or use of such information is strictly prohibited
> and may be unlawful.  If you are not the addressee, please promptly delete
> this message and notify the sender ofthe delivery error by e-mail or you
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