[Histonet] (no subject)
Hi there, Any recommendations for a ductless, tabletop, fume hood (roughly 27 wide x 22 deep x 13 high)? I have done searches online, but would appreciate any recommendations. Thank you!Rhonda McCormick Medical laboratory supervisor CVM Histology Diagnostic Laboratory, Texas A University College station-TX-77843, TAMU -4467 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Long term museum specimen storage
Hi All, I am looking to replace the fixative for veterinary specimens that have been preserved as "museum specimens". They are kept in jars in a glass case outside our lab, however, some of the fixative is starting to turn brown (and we've pulled a few jars that have some slight cracks in them). The specimens are currently in Jore's Fixative: 100 mL Distilled water 10 mL 40% Formaldehyde2 g Magnesium Sulfate2 g Sodium Sulfate1 g Sodium Chloride Preserving specimens is new to me. I've never heard of Jore's fixative before and I'm wondering if I could get some advice, please? Do these specimens need to be replaced with the same solution? Could we rinse the specimen and replace the solution with 70% Alcohol? OR would 10% NBF be better to store the specimens in (or something al together different)? We have a varying display of specimens - anywhere from a small porcine optic nerve to a large equine granulosa cell tumor. Realizing it may be different based on the size of the specimen, approximately how often should the solution be changed ? Thank you so much! Any help or insight is much appreciated. Rhonda McCormickRhonda McCormick BS, HT (ASCP)cm Histology Diagnostic Lab Supervisor College of Veterinary Medicine Texas A University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gram Stain
Howdy,Does anyone have a recommendation for a Gram stain (or modification of a gram stain) that stains the background yellow with red gram-negative bacteria (no blue - gram positive staining).Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram negative bacteria. If so, would you mind to send your protocol please? Any recommendations for a gram negative (only) stain - specifically E. coli? One of our residents is asking. Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AP
Would anyone please share any information regarding staining FFPE tissue with Alkaline Phosphatase for osteosarcomas. Is it solely performed by IHC or are there any special stains that could be used?TIA Rhonda McCormick BS, HT (ASCP)cm Histology Diagnostic Lab Supervisor College of Veterinary Medicine Texas A University Lab: 979.845.5149rmccormic...@tamu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Crusting lesions and thick cattle skin
Hi!I'm looking for recommendations for cutting FFPE blocks that contain thick cattle hide/skin and crusting lesions. What do you all use to soften this kind of tissue? Our lab has used Millifex, fabric softener, and nair. We read ammonia may work to rehydrate the tissue enough to get a decent section. Has anyone tried that?Thanks in advance for your help!Rhonda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MelanA
I thought I'd reach out and see if anyone can help with some IHC troubleshooting (or know someone who might). Our Melan A that once was working on our canine tissue, no longer is. We are using: 1:300 dilution of the Leica MelA antibody BioCare IHC platform Antigen Retrieval performed in pressure cooker Alkaline Phosphatase detection We are also using known controls and still... no staining. We'd appreciate any ideas or recommendations (or even questions that might lead to those "lightbulb" moments). Sometimes when you're so mired in the problem, it just takes someone else to shed some light on it. Thank you Rhonda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Help! I became HT certified in 2004 under the HS degree and lab experience qualification. I do have a Bachelor’s Degree in Education,, with only 12 hours of science. I am looking to relocate to Texas and therefore am job hunting. The problem I’m running into is that without an Associates Degree or Bachelors in a Science related field, I am not being considered for job opportunities even though I have 17 years of experience (and am a good histo tech and good employee with great references). Is anyone else running into this problem? Does the Histonet world recommend I go back to school - or just be patient, trusting a job will eventually come along? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
