[Histonet] revised Cap regulation ANP .21395 ??
Hello Histonetters, What is the take home message of this revision? How are other labs complying with respect to Special staining? Do you keep records of individual case control verification or just individual block verification? Does anyone run NEG controls for Special staining? Is this regulation requiring Neg Controls for special stains? Any thoughts are appreciated. " **revised** 08/17/2016 ANP .21395 Special Stains/Studies For special stains, including histochemical stains, and studies using immunologic and ISH methodology, positive and negative controls are verified and recorded as acceptable prior to or concurrent with the reporting of patient results and records maintained. " Thanks you, Ryan Roy BS, HTL (ASCP)CM Laboratory: Histology Department Manchester VA Medical Center Manchester NH 603 -624-4366 extension 6640 ** Disclaimer: The content of this email does not reflect the views or opinions of the Veterans Administration*** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Syringe Pump error Ventana??
Does anybody know what the Syringe Pump is on the Ventana Benchmark Special Stainer?? What does it refer to in layman's terms? The operators manual is really vague, unless I missed a page? Ryan Roy BS, HTL (ASCP)CM Laboratory: Histology Department Manchester VA Medical Center Manchester NH 603 -624-4366 extension 6640 ** Disclaimer: The content of this email does not reflect the views or opinions of the Veterans Administration*** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Aperio CS2
Anyone out there working with Aperio CS2? How does it function for you? We want to use it for consultations. Ryan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histology AND incinerators?
Are any histology labs out there using incinerators for decontamination of lab forceps? We are setting up to begin using Germinator 500 at both gross and embed center, was wondering if any other labs have used this type of technology, any insights helps. Thanks in advance, Ryan Roy HTL (ASCP)CM Manchester VA *** Disclaimer: NO content in this email reflects the opinions of the Veterans Aministration. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Cap cross contamination at gross bench
Anyone out there see the new CAP requirement regarding cross contamination at gross bench and cleaning of forceps. How are other people planning to comply. My supervisor has instructed me to use a Germinatior 500 at gross bench and at Embedd center. I have no experience using these sterilizers but my initial thought is that I will need to be really careful not to inadvertently apply too much heat to the tissue. Any thoughts appreciated, Ryan Roy HTL (ASCP) Manchester VA Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [EXTERNAL]
Hello, I wish you had contacted me a few months back. Management moved a tech over internally here. I will put you in contact with White River Junction VAMC . They may have a postion open. Also, I would check University of Vermont Med center;, Brattleboro Memorial hospital, Dartmouth Medical Center, Catholic Medical Center, Manchester. I know people at all these locations, often times a latent position (everyone knows about it ) won't post until a certain period so you wouldn' t know its there without asking. That said, Ill ask around. Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opinions of the VA. From: Kaylie Grenier [mailto:kgreni...@student.goodwin.edu] Sent: Tuesday, May 17, 2016 11:42 AM To: Roy, Ryan Subject: [EXTERNAL] Good morning, My name is Kaylie Grenier, I am a histology student at Goodwin College. Kelli Goodkowsky gave me your email address so I can get in contact with you. I am in my final semester of the program and I am hoping to relocate to New Hampshire at the end of August and am planning on taking my HT certification as soon as I am eligible. I was just wondering if you knew of any potential positions up in NH or if you knew of anyone I could get in contact with. I will be moving to the New London, NH area. Thanks! -Kaylie Grenier ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Salutations from former GC histo sci student
Hello Kelli, You may remember me as one of your online graduates from the GC histological science program. How are you? For some reason I thought your involvement with the GC histo program had changed, but seeing your recent post to the histonet it appears your still the director of that program? I hope things are well. You were instrumental in helping me complete that program successfully and were also a good instructor. Ive been at the VA Manchester NH for 2 years now. I am the lone histotech here since the other technician left. Things are going great though we have a critical need for another qualified technician. Please let me know if any of your students especially those already holding a BS would be interested in employment at the VA Hospital in Manchester NH. I could certainly guide them through the application process. Thanks a ton, Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opinions of the VA. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology position
Hello Histonetters, The Manchester VA Medical Center in Manchester NH currently has a critical need for a qualified Histologist. It's an excellent position with great benefits. If you are interested please review the link below. Any further questions can be directed to contact Kevin Hall at (603)624-4366 ext. 6638. Feel free to share the link. https://www.usajobs.gov/GetJob/ViewDetails/422133100 This is not a third party posting. Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opinions of the VA. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] recycled alcohol versus reagent grade alcohol
Hello Histonet, How do other labs test and verify the percent grade of alcohols and what is the assumed percent grade of reagent alcohol? We use recycled alcohols but today based on not having reagent grade we used 97% tested recycled as our flush. The reasoning behind this was that Reagent grade varies from 95%-greater than 100%. Is this true? Thanks in advance, Ryan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sections blowing apart in stainer
Hello, Cut some skins the today (3 sections one slide) and the top two sections seemed to blow apart and drift in the stainer (not on the bath). The bottom section looks good. This is strange as I have seen the bottom section move as a result of water or not adhering to slide. However is in this case the top two sections appear blown apart and drifted and this occurred while staining? Any thoughts appreciated. Thanks, Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opions of the VA. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CBG recyler and recycled Formalin
Hello, We are getting a new CBG that recycles xylene , alcohol, and formalin. We purchase buffered formalin? Does anyone know if after recycling the recycled formalin would or would not need be re-buffered?? Thanks in advance, Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opions of the VA. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] Re: [Histonet] gelatin
That's interesting, I didn't realize gelatin is the water soluable protein of collagen. No experience with staining gelatin, but have you considered MassonTrichrome. It is used to differentiate collagen from smooth muscle... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Tuesday, February 24, 2015 12:48 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] gelatin You need to explain treated tissue. Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the HE method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang ynw...@u.washington.edu wrote: Hello, Does anyone know of a stain specific for gelatin? I would like to distinguish between firbous collagen and gelatin in treated tissue. thank you Yak-Nam University of Washington Seattle, WA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CBG recycler
Hello histonet, Has anyone out there dealt with a busted Heat Resistor in a CBG reagent recycler. Any thoughts or insights appreciated. Thanks in advance, Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Contiued Ed HTL
Hello, Anyone have any suggestions CE courses? This is my first time working on these as I am a new HTL. Thanks in advance, Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] Re: [Histonet] Re: Embedding
I use a block trimmer. I think it is safer for wrists. Being that you push the block against it and I seemed to always pull when I scraped blocks. I am guitar player and need my wrists. I don't think it is a strong argument to say its faster then hand scraping atleast not until a good technique is developed by the tech. Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opions of the VA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, January 22, 2015 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Re: Embedding That is so cool!! I wish I had one of those!! By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Wed, Jan 21, 2015 at 1:59 PM, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: We also use the para-trimmer. In my view, it is worth its weight in gold! I can melt 5 blocks at a time, works like a charm. I am one who does not mind the wax on the sides, as I am most confident that there is enough paraffin to support the cassette. Michelle Sent from my iPhone On Jan 21, 2015, at 11:41 AM, Morken, Timothy timothy.mor...@ucsf.edu wrote: I agree. We got one of these a couple years ago and the techs love it. It is a heated block on which you rub the cassette. The paraffin melts away. It is especially good for preserving barcodes (but don't press the printed surface on the heat block too long - you can soften the print and cause some damage, but nothing like can happen with scraping). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, January 21, 2015 8:29 AM To: gayle.cal...@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest price by far - and are saving our finger joints. The amount of wax remaining on the cassette also appears to depend on the brand of mold used. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 21, 2015 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] RE: [Histonet] cutting honey bees
I agree with Patsy. If you have a access to a plastic histo lab, that would be the best, as you could easily section any part of the Bee's anatomy. Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 2:13 PM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: [EXTERNAL] RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Formic acid disposal?
Hello, We Label our waste containers with the type of waste, waste code, and date of collection. Then a waste company picks up as necessary. Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Monday, January 05, 2015 11:28 AM To: Johnson, Carole; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Formic acid disposal? Our waste disposal company has us combine our formic acid waste with our special stain/HE stain waste. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Carole Sent: Monday, January 05, 2015 5:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formic acid disposal? Hello everyone and Happy New Year, Can anyone who is using formic acid in their lab tell me how they handle/dispose of it after use? I realize there is some variability by region, but any help would be appreciated. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Formic acid disposal?
I got this to.. Can an administrator explain or delete the source? thanks -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Monday, January 05, 2015 1:22 PM To: remove Subject: [EXTERNAL] FW: [Histonet] RE: Formic acid disposal? I keep getting these emails. Since I can't read in whatever language this is I don't know what it says. They are asking for verification. What does this mean? In this day I don't just go putting my info out there to anyone. Is this person legit? Is anybody else getting these? Andi G. From: moine...@mokpo.ac.kr [moine...@mokpo.ac.kr] Sent: Monday, January 05, 2015 11:13 AM To: Grantham, Andrea L - (algranth) Subject: RE: [Histonet] RE: Formic acid disposal? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/logo.gif] ★스팸메일차단을 위한 인증 서비스 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] 편지 감사합니다. 저는 moine...@mokpo.ac.kr 이메일주소 사용자 입니다 국립목포대학교 웹메일은 스팸방지를 위해 인증서비스를 시행하고 있습니다. 따라서 저에게 편지를 보내시려면 처음 한 차례의 인증이 완료되어, 저의 주소록에 귀하가 등록이 되어야만, 저에게 정상적으로 메일을 전송할 수 있습니다. 본 인증 이후 저의 주소록에 등록되면,그 후 발송되는 메일은 인증과정이 생략됩니다 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button.gif]http://mmp.mokpo.ac.kr:8886/mmpmgr/user/auth_view.php?msgid=54AAD4507E25 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] 경고: 30일 이내에 인증번호를 전송하지 않을 경우, 보관된 메일이 삭제되오니, 본 메일 확인 후, 꼭 인증번호를 입력해 주시기 바랍니다. [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] ★Confirmation Service for Spam free [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] Thank you for your email This is moine...@mokpo.ac.kr Please complete the verification so that I can receive your email. “After finishing this verification, you can be in my address book forever and won't need to do it again.” [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button_1.gif]http://mmp.mokpo.ac.kr:8886/mmpmgr/user/auth_view.php?msgid=54AAD4507E25 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] Warning: you must c_img to deliver the mail, or it will be droped in 30 days! [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/bottom.gif] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] THICK AND THIN SECTIONS ?
Probably, Also, just because a manufacturer doesn't sell a part doesn't mean you can't get a part somewhere else. Ryan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, December 16, 2014 10:00 AM To: Klaus Dern; histonet Subject: [EXTERNAL] RE: [Histonet] THICK AND THIN SECTIONS ? This is at least the third time I have seen this message. Is this a form of advertisement on the Histonet? Grumpy in San Diego because it is supposed to rain today. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Klaus Dern [klaus.der...@gmail.com] Sent: Tuesday, December 16, 2014 6:01 AM To: histonet Subject: [Histonet] THICK AND THIN SECTIONS ? If you are using one of the following Microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG2035 LEICA - CM 1850 Cryostat SAKURA SRM200 You could be faced with purchasing a new Microtome because these models are no longer supported by the manufacturer. ( No parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to fix this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.der...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] Re: [Histonet] Water baths
Hello, I agree, we record daily all our temperatures for all equipment in histology. Ryan Roy HTL (ASCP)cm Veterans Affairs Medical Center Manchester NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Tuesday, December 02, 2014 10:47 AM To: Abbott, Tanya Cc: Histonet Subject: [EXTERNAL] Re: [Histonet] Water baths Speaking for myself and the labs I have managed, I have been checking ALL temps and recording them since the early 90’s. These include waters baths for tissue flotation, and ones used for special stains, ovens, refrigerators, freezers, cryostats, tissue processors, embedding centers, decloaking chambers and room temps. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.commailto:aalli...@montereygi.com Tele: (831) 375-3577 X117 On Dec 2, 2014, at 7:29 AM, Abbott, Tanya tanyaabb...@catholichealth.netmailto:tanyaabb...@catholichealth.net wrote: Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.netmailto:tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] Re: [Histonet] fixative
I agree that alcohol is also less toxic. What type of tissue are you fixing? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, November 25, 2014 10:46 AM To: Schade, Adelle Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] fixative What are you going to accomplish? Alcohol will work as a fixative and dehydration solution. Less of a disposal issue. William DeSalvo william.desa...@sonoraquest.ccom 602-768-3692 Sent from my iPhone On Nov 25, 2014, at 8:30 AM, Schade, Adelle a_sch...@conradweiser.org wrote: Hello, I am considering the following fixatives for a high school histology experiment and I would appreciate any input considering safety, disposal, etc. 1- Ultrum II (American MasterTech): promotes disposal in local sewer? 2- Excell Plus (American MasterTech): low-hazard but any idea on disposal from those who use it? 3- Histochoice Tissue Fixative Anyone using these products/ advice is greatly appreciated! Ms. Adelle L. Schade, B.S., M.Ed. Anatomy and Physiology Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 610-693-8599 x6736 a_sch...@conradweiser.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Specimen numbering systems
I agree with Dawn . Basically use a numeric for the Block and an Alpha for the specimen. This way a large resection specimen would receive an A and all blocks for said specimen would be labeled as A1,A2,A3...ect -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Monday, November 24, 2014 2:26 PM To: 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [EXTERNAL] [Histonet] RE: Specimen numbering systems We do it the same as Toni and from the CAP Uniform Labeling of slides and Blocks presentation I attended at NSH that is what CAP came up with for uniform labeling (S14-1234 A1, A2, A3, B1, B2, etc...). Dawn Schneider, HT(ASCP) Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, November 21, 2014 2:18 PM To: 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We use a numeric for the accession number (S-14-1234), followed by alpha for the specimen(A, B, C), and within that have numeric for the number of blocks in each specimen (A1, A2, A3). We haven't ever gone to Z in our current LIS, and the ability to add unlimited blocks to each specimen makes it easy to deal with. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, November 21, 2014 3:11 PM To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We are currently using Numeric for the specimen (or part) and Alpha for the block. I don't like it; we frequently have 1Z blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended
