[Histonet] RE: P16 and HPV
WHO is going to discontinue HPV testing? Dana Settembre University Hospital -UMDNJ Newark, NJ USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Tuesday, September 25, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 and HPV I've been told that the HPV testing will be discontinued as of end of year. HPV expresses the P16 gene but so does other type of cancers associated with HPV. It is possible to have a P16 pos and HPV negative test. But is it not possible to have HPV pos and P16 neg. if HPV is positive then the P16 is also positive. So the P16 cannot be used to replace the HPV testing can it? Antoinette Crill TEAM LEADER ANATOMIC PATHOLOGY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls and CAP
Hi Chris, CAP actually has a specific checklist question on Negative Controls. The question is ANP.22570 and if I remember correctly it is quite extensive. Most of the CAP questions are about 7 to 10 lines. The Negative control question is more than a page long. Get your hands a copy of the most current checklist for anatomic pathology - That will include Immunohistochemistry. By the way, the rule is that a negative control must be included with each run and there is much, much more. ANP.22570 Good Luck, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Friday, July 13, 2012 9:31 PM To: histonet@lists.utsouthwestern.edu Cc: cjac...@clinpath.com Subject: [Histonet] Negative controls and CAP Histonetters, I have been made the IHC lead in a fairly large laboratory that is seeking to become CAP accredited. I am definitely a newbie when it comes to CAP. A question was brought up today about how we do our negative controls. Specifically, should we run another negative control with any IHCs that need to be repeated or if we should run another negative control if at a later date a pathologist orders additional IHCs on a case. With our current protocol, we run one negative control per detection kit used, per case. Consequently, most cases end up with just one negative control slide. We do NOT run another negative control if we have to repeat one of a group of immunos or if a pathologist orders additional stains at a later point. I am wondering if this is good practice, and acceptable with CAP. I would love to find some literature that could help me make a case either way on this. Actually, any literature or pointers regarding IHCs and becoming CAP accredited will probably help save what little hair I have left. Thank you, Chris Jacobs, HT(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Re: Methanol in H2O2 explanation
Carl, H202 mixed with Buffer here.(instead of aq.) That's OK too, right? Dana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Tuesday, July 10, 2012 11:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Methanol in H2O2 explanation Why do some people use methanolic H2O2? Why do people rehydrate after dewaxing? Both are unnecessary, under usual conditions. Methanol was used in the early days as a peroxidase blocker by itself. The combination was devised as a belt and bracer method. As you stated, you use aq H2O2 effectively. So do I and many others. However, for unfixed frozen sections, I would never use aq H2O2, if I wanted sections remaining on my slides After dewaxing, rinse sections in 4x 100% alcohol, rinse in water, endog. Px block while you make up you AR solutions Carl Hobbs Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Happy Retirement to Jim Burchette
Best of Luck to Mr. Burchette. Who, I am sure, doesn't know me from Eve, but once sent me a positive control block. I didn't ask for anything in return. I was happy astonished. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Happy Retirement to Jim Burchette I am making this official and inviting all of you who know Jim to join me in wishing him a very happy retirement. His impact to our field and to the patient care at Duke University has been stellar. I had no idea his feet were so big gauging by the size of shoe left behind to fill. Best wishes JB! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking p16 - where is mtm labs?
Looking to get current pricing and availability from mtm labs on p16 and no one answers their phone - 800# nor their direct# Seems Roche has taken them over. Need to reach them. Any help would be appreciated Dana Settembre Immunohistochemistry University Hospital - UMDNJ Newark, NJ USA Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] THANKS for p16 info
From: Amanda Kelley [mailto:akell...@slu.edu] Sent: Tuesday, June 12, 2012 12:13 PM To: Settembre, Dana Cc: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] looking p16 - where is mtm labs? You have to get current pricing on P16 from your local Ventana rep. It is available both in the vial and in the Ventana dispenser. Amanda On Tue, Jun 12, 2012 at 11:05 AM, Settembre, Dana sette...@umdnj.edumailto:sette...@umdnj.edu wrote: Looking to get current pricing and availability from mtm labs on p16 and no one answers their phone - 800# nor their direct# Seems Roche has taken them over. Need to reach them. Any help would be appreciated Dana Settembre Immunohistochemistry University Hospital - UMDNJ Newark, NJ USA Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. Grand Blvd. St. Louis Mo. 63104 Phone: (314) 977-7868 Fax: (314) 977-8740 akell...@slu.edumailto:akell...@slu.edu DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of Saint Louis University. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP Question -ER/PR Validation
You should pose that question to CAP via acc...@cap.org they answer quickly and when they do you can print out their answer and keep for when your inspectors come. When I asked them that question, they said that it was usually at the discretion of the pathologist in charge. But instead of taking my word for It, email them and when you receive their response, print it. Then follow their instructions. Dana Settembre -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Monday, April 16, 2012 11:34 AM To: Histonet Subject: [Histonet] CAP Question I have a question regarding a new CAP checklist question dealing with ER/PR validation. The new question is ANP.22976 ER/PgR Validation and states: If the laboratory performs immunohistochemistry for estrogen receptor (ER) and/or progesterone receptor (PgR) as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assay(s). Note: Initial test validation should include a minimum of 40 cases (20 positive and 20 negative cases) for FDA-approved/cleared tests; laboratories should consider using higher numbers of test cases if a Laboratory Developed or Laboratory Modified Test is to be validated. Validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. Acceptable concordance levels are 90% for positive results and 95% for negative results. If significant changes are made to the testing methods (e.g. antibody clones, antigen retrieval protocol or detection system) revalidation is required. Our original validation was done in 2005 and was performed with 20 cases. Do we need to revalidate to be compliant with this question? Should we start from scratch or just do an additional 20 cases? Any feedback is appreciated. Thanks! Schaundra Walton BS HTL(ASCP) Histology Supervisor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New Reagent Lot Verification
We are saving slides from inspection to inspection, about 2 years. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, April 12, 2012 11:26 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We're saving both for at least 2 years. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be Records of verification of new reagent lots. What is everyone's interpretation of records? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Does xylene cause skin cancer?
I would be worried too. Wearing gloves in the lab is always a good practice and I believe, a requirement in my lab whenever handling reagents or the possibility of coming into contact with reagents. You have the right to wear gloves if you want. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Tuesday, February 21, 2012 10:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does xylene cause skin cancer? I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC and negative controls?
Tom, As Tunde says, the Checklist 7/11/2011 question ANP.22570 does explain that you can do as your pathologists suggests, BUT there are rules to be followed if that is what you will do. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks ... 2. The positive control slide or the patient test slides, if these slides contain tissue elements that should not react with the antibody. [this is the one you want to follow] 3. A separate negative tissue control slide. The type of negative tissue control used ... SHOULD BE SPECIFIED in the laboratory manual. ... Evidence of Compliance: Written procedure of the selection and use of ... tissue controls for IHC... Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tunde Ajibade Sent: Thursday, February 16, 2012 10:40 AM To: 'histonet@lists.utsouthwestern.edu' Cc: 'histonet-boun...@lists.utsouthwestern.edu' Subject: [Histonet] RE: IHC and negative controls? I am not sure if they can do that because CAP ANP.22570, explain this. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 16, 2012 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and negative controls? Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MYO-D1
Hi Donna, I use Dako's Myo-D1 cat. M3512. Use it at 1:25 I use Dako's labeled polymer kit, Envision + Mouse. Use Trilogy (from Cell Marque) in a steamer. That means: Start the steamer and put a two Coplin jars in filled with Trilogy. Place all your slides in 1st Coplin jar for 30 min. right then, at RT. The temp will go up to 95 degrees as the Trilogy acts as a deparaffinizer. Then put he slides in the 2nd Coplin jar for 30 min. and Trilogy acts as AR. Good luck. Dana Settembre University Hospital - UMDNJ Newark, NJ 07103 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna Liaros Sent: Friday, September 09, 2011 4:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MYOD1 Any protocol recommendations for MYOD1? I am having a difficult time in trying to work up this antibody. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP slides
Her-2 IHC Dana Settembre -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Coppin, Margaret Sent: Wednesday, August 31, 2011 5:53 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP slides Hello, A few months back, there was a discussion on the list about CAP Proficiency Test slides that weren't staining as expected. Some of you had even contacted CAP and they acknowledged that there had been an issue. Any of you whose PT was affected, do you mind jogging my memory as to which PT's were the problem batch? Thanks in advance as always. Margaret --- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: pten Antibody
Leica sells the NovoCastra line pTEN. That's what I have been using. Which vendor have you been using? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Monday, August 15, 2011 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pten Antibody Can anyone recommend a pten antibody for use in FFPE? Antibody I was using is no longer available Thanks Luis Luis Chiriboga Ph.D OCS Experimental Pathology IHC Core Lab Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 luis.chirib...@nyumc.org /PRE html body br / This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.br / = /body /html PRE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: TFE-3
We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: P16 on the BOND
Hi Nancy, I am using the p16 with the Bond. I am using the Bond's ER1 for 20min with their Refine detection kit. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Monday, July 18, 2011 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 on the BOND Happy Monday- I should have been more specific in my question - is anyone using p16 on the BOND IHC instrument? Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: P16
The only company currently allowed to sell it (have it, etc.) MTM Labs 1-866-686-5227 CAT#: 9581 Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, July 14, 2011 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 What are folks using for P16? Thanks for your help- Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase
This is so much fun. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, June 30, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu; McAuliffe, William Subject: Re: [Histonet] Luciferase You can, but the retrieval involves a young priest and an old priest. ff McAuliffe mcaul...@umdnj.edu 6/30/2011 12:42 PM It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: ok there is a really BAD religious joke coming soon, isn't there? Rene J Buesarjbu...@yahoo.com 6/30/2011 12:00 PM You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu, 6/30/11, sgoe...@mirnarx.comsgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.comsgoe...@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PAX-2
Want to purchase Pax-2 for FFPE human tissue. I have been told that there is a countrywide issue that has it on BACKORDER - I have been waiting for about a 3 to 4 weeks. I have been told that a release date may come in a couple of months. I. Does anyone know enough to confirm this? II. Where do you get yours? Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ 07103 USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC verification forms
Validate. Dana Settembre University Hospital - UMDNJ Newark, NJ 07103 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, June 16, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC verification forms Please help me understand, do we need to verify antibodies or validate them, or is it essentially the same thing? Also, can I please get a couple examples of the forms you might be using, perhaps email me a copy??? I'm still new to the IHC world and trying to get things inline as they need to be. Thanks, curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Her2 CAP survey
Laurie, when we called CAP they also told us to use Code 11. That's a GOOD point - specimen unsatisfactory is this issue. Dana Settembre -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 11, 2011 11:16 AM To: Richard Cartun; Jayne Halli; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Her2 CAP survey Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for unable to analyze (11), and to write an explanation on the last page. My pathologist is questioning why we wouldn't mark specimen unsatisfactory (33). What are others that had light staining doing and/or what have you been told by CAP? CAP also told me that they didn't have any replacement slides to send out. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 05, 2011 1:06 PM To: Jayne Halli; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Her2 CAP survey Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Jayne Halli jayne.ha...@avera.org 5/5/2011 3:01 PM Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.ha...@avera.org - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cap new ANP.22760
ANTIBODY LOTS I have a Dako and I have a Bond too. For the Dako I use my positive control. I use 3 slides. One is treated as the negative control - no antibody One is treated with the current lot One is treated with the new lot. I keep all QC slides together. I keep the 3 slides together. For my Bond, I use 2 slides of my positive control When a new lot comes in I run the new lot and the negative control. Then I compare them to the previous lot that I ran in the past. I keep the slides in chronological order - the previous lot goes back with The slides from that date. The NEW Lot slides get filed with today's date. DETECTION SYSTEM LOTS For the Bond I QC the detection kit using an antibody like Ki-67, it works well and always shows good nuclear staining. I indicate the received date, and QC date. I run the QC on my Ki-67 positive control. I use 2 slides. One is treated as the negative control - no antibody. One is treated with Ki-67. I compare these to the previous Detection kit QC slides I created a written procedures for all of the above. Hope this helps. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Friday, April 29, 2011 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap new ANP.22760 hi everyone, just wondering how others were handling this checklist statement? thanks so much for your input. seems like a lot of extra work when running control with each show if each is working. anita dudley providence hosp mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pax-2 on FFPE human tissue
Hi All, Having a tough time looking for a reliable vendor for Pax-2. What vendors are you using? I need to use this on formalin fixed paraffin embedded human tissue. What control do you use? Thank you. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lab week
Lab Week is April 24th through April 30th. Dana Settembre -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yahoo Sent: Monday, March 28, 2011 9:26 PM To: Histonet Subject: [Histonet] Lab week Can someone tell me when lab week is? Thanks in advance. Sent from the iPhone of Kim Tournear ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: negative controls on immunos
Yes, you should run negative controls with each run - today and tomorrow. Something can happen in tomorrow's run so tomorrow's negative control will have different issues from today's negative control. The negative control should go through everything that your slides go through, whether it's today's great run or tomorrows electrical black out or whatever mishap. Whenever you run a test you must have a positive control and a negative control. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ 07103 USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Tuesday, March 01, 2011 1:30 PM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls on immunos We do not run additional negatives with each run on the same block. If it was a different block then yes we would run a negative. It takes up too many spaces on the machine and uses reagents. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmcc...@ckha.on.ca] Sent: Tuesday, March 01, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls on immunos If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CD21 antibody
Hi Linda, I found Dako's CD21 mouse antibody reliable. M0784 Dana Settembre University Hospital - UMDNJ 150 Bergen Street Newark, NJ 07103 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, February 07, 2011 1:16 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CD21 antibody Can anyone out there in Histoland recommend a reliable and robust CD21 for FFPE human tissue? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any problems with Bond Max Heater Assembly (SSA)
Has anyone had any problems with the Leica Bond Max's Heater assembly. We have 2 Bonds that are less than 2 years old and one of the heater assembles went. That is 10 less slides that we have to work with. Thank you, Dana Settembre University Hospital - UMDNJ Newark, NJ 973-972-5232 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, January 28, 2011 10:06 AM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] LEAN Processes/ new lab Hi Nancy, We built a brand new pathology lab from the bottom up last year. Drop me an email off line or give me a call and we can discuss our lessons learned etc. I worked with a LEAN workflow specialist on the design and equipment placement prior to the plans being approved. We included the architect in all of out discussions about this. You can always come to snowy New Hampshire and check it out yourself? Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, January 28, 2011 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEAN Processes/ new lab Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ihc slides
Hi Cindy, That too has happened to me and the process that you do to remedy it, works for me too. (take off coverslip properly, re-apply DAB, and you get a reaction.) My experience is that the slides are not shot, as described by another Histonetter. There was definitely something else going on. I work with the Dako too. Is it possible that the Autostainer was not programmed to apply antibody in the crucial zones? Look into that possibility. Good Luck. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Monday, January 24, 2011 7:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ihc slides Happy Monday Histonetters I need to pick the brains of the IHC experts out there in histoland. I run IHC on the Dako autostainer. On one of our runs there was not enough DAB to dispense on all the slides. My tech dehydrated the slides and cover slipped them. We printed the stain log on the run and confirmed that the DAB was not dispensed on three slides. The cover slips were removed and the slide were soaked in xylene to remove the cover slipping media. The slides were rehydrated and DAB was applied. There was still no reaction after 10 minutes, The DAB was reapplied for up to 30 minutes with no reaction. Can anyone out there give me a reason that the slides would not react after the reapplication of the DAB. I have done this before when the DAB solution was short on the run and it worked. I can't figure out why this didn't react. I called tech service at Dako and talked to the supervisor, she agrees with me that the reapplication should have worked. I checked the run log and the only missing step is the DAB. The antibodies with the missing DAB have their own controls on the slides. Any thought out there as to what the problem is. I have had the PM done on the Dako which should resolve the problem according to Dako. There has not been a problem since. This mystery will drive me crazy until I can figure out why. I could use help. Thanks in advance, I appreciate any input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MPB antibody
Hi Jean, I use Leica's MBP which the get from NovoCastra cat. NCL-MBP I use it on formalin fixed paraffin embedded human tissue on my Dako. I use Dako's Target Ret. Soln in a steamer for 40 minutes Incubate 15 min with it at the dilution of 1:25. Then I use Dako LSAB2 kit. It works fine. Good luck, Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, December 23, 2010 2:14 PM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] MPB antibody Hi Everyone, Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ISH edge effect on TMA
Has anyone experience edge affect on TMA when doing ISH? Dana Settembre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Kathleen Dittrich, where are you?
Kathleen Dittrich, formerly from Dako. Where are you? Dana Settembre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Edge affect on TMA?
Does anyone experience edge affect on TMA? Dana Settembre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problems with staining IHC
Are you staining by hand or are you automated? Dana Settembre University Hospital Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 10:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 antibody
Hi Michele, Yes, it's true, only MTM labs sells it and they Sell it as a kit and they also sell it along with a negative control reagent. That's how I buy it. They have a license or something and no else can sell it now. Their cat# is 9518 and I think it's called the CINteck Histology V-kit, (you should double check with them.) They will make you open up an account... Good Luck Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr Sent: Thursday, October 07, 2010 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this antibody that I could use on the bond autostaining machine. Thank you, Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
