Re: [Histonet] Advice for new grads?

[Victoria Baker via Histonet]

Pam,

So far a lot of excellent advice has been given. Along the lines of what
Tim has said, learn new tests. Any chance to get scope time with a
pathologist one on one or in a multi scope setting is an excellent learning
tool. It shows your interest & you will get to know your pathologists. This
will also encourage them to feel more comfortable in the lab.
Try different lab settings. Academic research you have a lot of interaction
with scientific people & you will have to figure things out/research them
yourself.  Clinical is where most people start out & what Tim said is spot
on. Pharma is really incredible, especially now. It will most likely mean
working with animal, cell culture & archival materials. You’ll need to
bring your A game to work each day, but most of the people in all these
settings will be willing to show an eager learner.
Good days come with bad days. It’s hard to not get discouraged, but
remember it is just one day.
Go get ‘em!

On Mon, May 9, 2022 at 1:16 PM Pam Barker RELIA Solutions via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Histopeeps!
>
> I hope this is the beginning of a fantastic week for you.  On Wednesday I
> will be speaking to a group of newly graduated histotechs.
>
> I wanted to ask if you could give someone just entering the field of
> histology a single piece of advice what would it be?
>
>
>
> Thanks-Pam
>
>
>
> Right Time, Right Place, Right Move with RELIA!
>
> Providing excellent service exclusively to the Histology Community!
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>
>
> Thank You!
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>
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> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
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>  
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Re: [Histonet] autopsy blocks

[Victoria Baker via Histonet]

Hi!

We are required to keep all of our materials for 20 years.

CAP I think is less, but we have to follow NYS.

Vikki Baker

On Fri, Jun 4, 2021 at 1:22 PM Nancy Schmitt via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello-
>
> Can you tell me what you are doing with autopsy blocks?
> How long are keeping these blocks/slides?
> Appreciated,
> Nancy
> Dubuque, IA
>
>
>
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Re: [Histonet] Question - Bone marrow core biopy specimens

[Victoria Baker via Histonet]

Hi Richard!

That is the duplicate of what we do in our lab.  We were putting both the
core and the clot on the same slide for in house testing, but den outs we
sometimes have to split the specimen and only on a slide.

I have a question for you about ISH Kappa/Lambda.  What decal protocol do
you use and do you see edge non specific detection/substrate on your
slides?  We use the Roche Iview blue.

I hope what I said helps.

Vikki

On Mon, May 3, 2021 at 4:12 PM Cartun, Richard via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello everyone:
>
> I'm wonder what your protocol is for cutting formalin-fixed,
> paraffin-embedded bone marrow core biopsy specimens?  Do you cut a limited
> number of slides upfront and then go back to the block if the pathologist
> requests histochemical stains or immunohistochemical tests?  We are
> implementing a protocol today where we will cut "15" slides upfront; 3 H
> at different levels and 12 unstains for potential histochemical stains
> and/or IHC tests.  Are we crazy?
>
> Thank you, and I hope everyone is doing well.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 (Office)
> (860) 545-2204 (Fax)
> richard.car...@hhchealth.org
>
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[Histonet] Frozen section QA

[Victoria Baker via Histonet]

Happy Monday morning!

We recently had an incident which led us to review our policy on frozen
section QA.
In your institution does the same pathologist who did the frozen section
read the permanent sections?  Also if they do then who is the pathologist
responsible for doing the QA?  How is this work flow completed?
Last, does any facility with less than 450 beds have their pathologists
specialized.

Thank you in advance for your help.

Have a wonderful week ahead!

Vikki Baker
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Re: [Histonet] QIHC Prep

[Victoria Baker via Histonet]

Ariel,

Go to the NSH learning they have a very good tool there.

Vikki

On Mon, Sep 14, 2020, 10:29 AM Ariel Liberda via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello all!
> I hope everyone is staying safe!
>
> I'm looking to get my QIHC and I was wondering what prep materials
> everyone found most useful? I've looked at the histonet archives and
> everything I found was mostly out of date. I'm also looking for info about
> the test itself. How difficult did you find it? Someone mentioned that they
> believed it was a test taken with notes available but I haven't been able
> to find confirmation of this. Any info will be helpful! I've worked with
> IHC's for a few years now so I'm looking forward to getting the official
> accreditation!
>
> Thank you all in advance! Histonet is such a wonderful wealth of knowledge!
> -Ari
>
> --
>
> Ariel Liberda, HT(ASCP)
> Lead Histotech
>
> CTALab
> c. [503.906.7300](
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Re: [Histonet] question about Digital Pathology Certificate

[Victoria Baker via Histonet]

Ranna,

I took the course and it was well worth the cost for me.  As to how it is
assisting my career right now it isn't as my facility stopped using imaging
about a year and a half ago.  With the pandemic they may be rethinking
this, but it won't be immediate.
This type of skill will be more important, I believe, with the advancement
of telemedicine and what we should be learning from the COVID-19 shut down
or limited resources of certain services.  Plus with so many facilities
merging to survive in the current environment this is going to be necessary.

What I would like to see is more follow up courses and more attention drawn
to this program.

Vikki



On Mon, Apr 6, 2020, 7:48 PM Ranna Mehta via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi All,
> I need inputs from my histo colleagues about Digital Pathology
> certification from NSH. If anyone has done this program, how is it helping
> in their career?
> what are pros and cons? Do they include MatLab  and R programming in
> syllabus? I know it is very resourceful in research lab but what about
> Clinical labs?
> Right now i am working on multiplex staining using Vectra inform and Halo.
> I am self learner; I don't have any help except company's technical
> support. I am wondering- attending this program will help me understanding
> algorithm and analysis? Thanks in advanced. Stay safe.
> sorry for lots of questions.
> Regards
> Ranna Mehta
> Associate Scientist
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Re: [Histonet] CPT Code 88172

[Victoria Baker via Histonet]

Hi,

I just referred back to my sometimes frustrating clear as mud 2019 CPT
coding binder.
88172 is not saying that it has to be done by a pathologist.  In many cases
it is completed by a Cytotechnologist.
The precise wording says
"Cytopathology, evaluation of fine needle aspirate; immediate
cytohistologic study to determine adequacy for diagnosis, first evaluation
episode each site
The professional code comes in on 88173 where it involves the
interpretation and report.

The stance that it is a tech component comes from the
Cytologist/pathologist obtaining the specimen.  However they are
responsible for the adequacy of of the specimen.  The argument goes both
ways.

At this institution we use it as a professional code only.

Vikki

On Wed, Sep 4, 2019, 11:43 AM Knutson, Deanne via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello Histonetters!
>
> I am wondering how others may be using the cpt code 88172-T?  Like in what
> type of scenarios?
>
> Thank you for your responses.
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
>
>
>
>
>
>
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[Histonet] Roche Symphony and Ultra users

[Victoria Baker via Histonet]

Happy Friday!

I'm looking to see if anyone out there is using these two specific
instruments together.

For about 8 or 9 months we have been experiencing what looks like water or
water droplets on the IHC slides.  It's not on all slides and we can go a
span of time with no issues only to have it come back.  It's not antibody
specific either.  We wash (Dawn dish detergent) and then load into the
Symphony.

I know there is the human factor, but right now I am just looking to see if
anyone is or has experienced this.

Thanks and enjoy your weekend.

Vikki
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Re: [Histonet] CD27

[Victoria Baker via Histonet]

On Mon, Jul 29, 2019, 9:45 PM Victoria Baker  wrote:

> Hi!
>
> Is anyone currently using CD27 on FFPE human tissue?
>
> I found one source and it is for lyophilized CD27.  I'd like to find one
> that is a concentrate.  I have given up on a pre-dilute.  I would like to
> see if there is both a mono and a poly clonal ab available and is I'm use.
>
> Thank you in advance for your help.
>
> Vikki
>
> Thank
>
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[Histonet] CD27

[Victoria Baker via Histonet]

Hi!

Is anyone currently using CD27 on FFPE human tissue?

I found one source and it is for lyophilized CD27.  I'd like to find one
that is a concentrate.  I have given up on a pre-dilute.  I would like to
see if there is both a mono and a poly clonal ab available and is I'm use.

Thank you in advance for your help.

Vikki

Thank
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Re: [Histonet] Use of Freeze Spray in a Cryostat

[Victoria Baker via Histonet]

Jennifer,

I called Leica as we have 2 cryostats at different locations so we needed
to be sure that we could use our current spray that we get from Leica.

We were told that their spray with the 1,1,1,2 Tetrafluoroethane is safe to
use.
I would therefore check to see what you have in stock and if it is this you
should follow the guidelines on the can.

We do not use spray on possible airborne pathogen cases and we avoid
spraying the specimen directly at all times.

Hope this helps.

Vikki


On Tue, May 28, 2019, 1:11 PM Schumacher, Jennifer J via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
> Looking for some documentation regarding the use of Freeze Spray in
> cryostats.
>
> A Best Practice document would be ideal, but in the absence of such a
> document, I would like to hear how others are planning to address the
> "recall" by Leica where they do not recommend the use of the Freeze Spray
> in their cryostats due to the possibility of a fire (flammable reagent).
>
> I personally have always had concerns around infection since the specimens
> are fresh and using the spray could aerosolize any infectious agents that
> are present.
>
> There is a lot of pressure to freeze things rapidly to meet the FS TAT, so
> the battle goes back and forth.
>
> Any feedback is much appreciated.
>
> Jennifer
>
>
> 
>
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[Histonet] ISH kappa lambda on bone marrow core biopsy

[Victoria Baker via Histonet]

Good morning!

Just recently we started having an issue with a heavy blue precipitate on
our bone marrow cores.  We have changed nothing in our protocol and even
did parallel testing with a reference lab.  Both had the precipitate, but
to a lesser intensity.

Has anyone else been seeing this?

Thank you in advance any and responses are appreciated.

Vikki
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Re: [Histonet] Air pocket in paraffin blocks

[Victoria Baker via Histonet]

Thank you everyone for your responses.  It is very much appreciated.

It is an up hill issue as most of the technical staff have been here and
maybe one other lab in their entire career and "no one's complained
before".  I'd like that phrase and "that's the way we've always done it"
stricken from all languages and any alien dialects we might ever encounter
in our lives.

Happy Friday and have a great weekend!

Vikki

On Fri, Mar 8, 2019, 2:38 PM Mayer,Toysha N via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Vikki,
>
> I teach students that they should not have any air bubbles in their
> blocks.  This means those under the lip of the cassette as well as
> surrounding the tissues.  Air bubbles can cause instability in the block
> especially if the tissue is hard or very small.  When they go to a clinical
> rotation no preceptor checks their blocks as closely as I do, but they can
> get a 'perfect' block without much effort.  Our tissues and cassettes have
> mostly been donated, so the quality may not be as optimal as that in a
> clinical lab, so if they can get a good block embedded with me, they can do
> it anywhere.
> It is actually a scored item on our graded checklist, along with the
> overall embedded time.
> You might want to do an in-service on air bubbles and give them a CEU for
> it.
>
> Hope this helps.
>
> Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
> Assistant Professor/Associate Program Director
> HTL Program
> MD Anderson School of Health Professions
> 713.563.3481
> tnma...@mdanderson.org
>
>
>
> Message: 1
> Date: Fri, 22 Feb 2019 18:32:41 +
> From: "deGuzman, Jose R" 
> To: Victoria Baker ,
> "histonet@lists.utsouthwestern.edu"
> 
> Subject:
> Message-ID:
> <
> by2pr13mb06966aaed227c932c333509196...@by2pr13mb0696.namprd13.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="utf-8"
>
> Hello Vikki,
>
> What you are experiencing is the lack of quality feedback. The
> "experienced" techs may have never been told that their quality needs to
> improve due to complacency or management is afraid they might leave. This
> gets worse if they have gotten away with producing poor quality for a long
> time and nobody has provided much needed feedback to them. You may have an
> uphill battle ahead of you but very much worth taking.
>
> Jose
>
> -Original Message-
> From: Victoria Baker 
> Sent: Thursday, February 21, 2019 5:49 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Air pocket in paraffin blocks
>
> Hi,
>
> When I was trained to do embedding there were many things that the
> professor stressed to me need to be done in order to have the tissue block
> acceptable for sectioning.  One of these was air bubbles.
>
> Recently we had a new tech embed a derm block that had an bubble that was
> pretty big.  The other (experienced) techs didn't think anything of it
> either and sectioned it.  When I got the block for IHC screening I made a
> QA form stating that the block should have been re-embedded before giving
> it to be sectioned, or when the first tech sectioned it could have repaired
> the block or melted it down.  This air bubble was big enough to be seen so
> I don't think it could have been missed - unless the block wasn't checked
> right after embedding.
>
> What has me a little upset is that no one seemed to care about this.
>
> I would really appreciate some feedback about this from other people.
>
> Thanks in advance.
>
> Vikki
>
>
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Re: [Histonet] Roche "bashing"

[Victoria Baker via Histonet]

Kelly,

It is not bashing, it is frustration and concern.

We need our instruments and reagents to work so that we deliver reliable
and consistent results to our pathologists.  They trust our work and
dedication to them and ultimately the patient.  When something like this
happens and we can't get a real resolution it undermines many things, but
trust is first for me.

Vikki

On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Such a wonderful insight into the thought process of Roche.  Accused of
> bashing when I am telling the truth about failed lots when they claim the
> problem is fixed.
> If only they would put that much effort into repairing the problem and
> fostering better customer relations, than how to market their product
> around this annoying truth.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
>
>1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley)
> Message: 1
> Date: Fri, 1 Mar 2019 13:15:58 -0500
> From: "Jordan, Kelley" 
> Content-Type: text/plain; charset="UTF-8"
> More bad press in histonet...not sure if we should pass it on to
> Marketing??
> @Will, Who as Teri Blaud at   Holy Redeemer Hospital ? Teri is always
> always bashing us.
> Kelley Jordan
> Strategic Account Manager - SC, NC, TN and KY
> A Member of the Roche Group
> Ventana Medical Systems
> Mobile:  803.504.1135
> Customer/Technical Support: 1.800.227.2155
> kelley.jor...@roche.com
> www.ventana.com
>
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Re: [Histonet] Ventana dispensers - last word

[Victoria Baker via Histonet]

Terri,

We went through hell with the Roche dispensers in late 2017 to early 2018.
The only thing positive I have to say is that the field engineer and our
FAS are great.  I have little respect for the sales staff or inside
support.

We pay top dollar for these instruments and the required reagents and all
Roche shows they are truly concerned with is the next sale

We also had a sit-down with their scientific people as well,  and here we
are again.  We haven't had the magnitude of failures this time but it is
disheartening to see that what they said they were going to fix has not
been fixed.

I was also wondering why they would send product failure notifications to
clinicians and not to the laboratories that are using the reagents.  I got
a notification from one of our oncology groups - but I never received one
in our lab.

Ventana doesn't exist and I miss that very much.  With each buy out,
merger or supposed partnership that I see with suppliers and manufacturers
things are turned more to the bottom line and in the end the patient
suffers and it's wrong.

But that's big business.

Vikki



On Mar 5, 2019 3:05 PM, "Terri Braud via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

To those of you who wrote in support of my comments, thank you sincerely.
To the Company's  credit, I did receive a call of apology and a listening
ear concerning the ongoing problems associated with the dispensers.  I
sincerely hope they understand the frustration that such a problem can
cause.
I want to state that I really do like the Benchmark Ultra, but the
instrument is only as good as the individual parts.  Based on many private
responses that I received, I think that dispenser failure is probably being
vastly underreported. I can only hope that a moment's indiscretion on their
part will lead to a more open dialogue in their company concerning a true
solution to this problem.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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Re: [Histonet] Air pocket in paraffin blocks

[Victoria Baker via Histonet]

Yes, it does. The air bubble went directly under the tissue.

Vikki

On Thu, Feb 21, 2019, 8:43 PM Jennifer Phinney 
wrote:

> Vikki,
> Did the air bubble affect the section quality on the slide? If not, would
> it have been worth the delay to the case in order to melt and re-embed the
> tissue?
>
> I only re-embed blocks with air bubbles if it will affect the quality of
> the slide myself.
>
>
> Jennifer
>
> -Original Message-
> From: Victoria Baker via Histonet 
> Sent: Thursday, February 21, 2019 4:49 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Air pocket in paraffin blocks
>
> Hi,
>
> When I was trained to do embedding there were many things that the
> professor stressed to me need to be done in order to have the tissue block
> acceptable for sectioning.  One of these was air bubbles.
>
> Recently we had a new tech embed a derm block that had an bubble that was
> pretty big.  The other (experienced) techs didn't think anything of it
> either and sectioned it.  When I got the block for IHC screening I made a
> QA form stating that the block should have been re-embedded before giving
> it to be sectioned, or when the first tech sectioned it could have repaired
> the block or melted it down.  This air bubble was big enough to be seen so
> I don't think it could have been missed - unless the block wasn't checked
> right after embedding.
>
> What has me a little upset is that no one seemed to care about this.
>
> I would really appreciate some feedback about this from other people.
>
> Thanks in advance.
>
> Vikki
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[Histonet] Air pocket in paraffin blocks

[Victoria Baker via Histonet]

Hi,

When I was trained to do embedding there were many things that the
professor stressed to me need to be done in order to have the tissue block
acceptable for sectioning.  One of these was air bubbles.

Recently we had a new tech embed a derm block that had an bubble that was
pretty big.  The other (experienced) techs didn't think anything of it
either and sectioned it.  When I got the block for IHC screening I made a
QA form stating that the block should have been re-embedded before giving
it to be sectioned, or when the first tech sectioned it could have repaired
the block or melted it down.  This air bubble was big enough to be seen so
I don't think it could have been missed - unless the block wasn't checked
right after embedding.

What has me a little upset is that no one seemed to care about this.

I would really appreciate some feedback about this from other people.

Thanks in advance.

Vikki
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Re: [Histonet] Closed doors in Histology

[Victoria Baker via Histonet]

Mike,

Yes that is correct.  Histology labs because of their toxic chemicals and
less than pleasant odors need to keep the doors shut for proper
ventilation.  This doesn't always happen when there is a high volume of
people going in and out.
I like it to the requirement that there be a sink (clean sink) in every
Histology lab too.  We have two sinks, 1 for special stains and the other
for tissue processor loading and formalin neutralization.  Which sink do
you think might be cleaner

Vikki Baker

On Fri, Nov 16, 2018, 11:35 AM Mike Pence via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> OK, How many of you keep your doors to your Histology Dept. closed all the
> time? We were told by a CIHQ inspector that Histology Dept must be under
> negative air flow ALL THE TIME. This was a new one for me. The standard is
> from the ASHRAE 170 table 7.1
> This is just an FYI for you all that these are the kinds of things CMS is
> looking at now days.
>
> Michael S. Pence | Supervisor of Laboratory Services
> Great River Health Systems
> 1221 S. Gear Ave. | West Burlington, IA 52655
> Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
> mpe...@grhs.net | www.greatrivermedical.org<
> http://www.greatrivermedical.org>.
> www.Facebook.com/GreatRiverHealthSystems<
> http://www.Facebook.com/GreatRiverHealthSystems> |
> www.Twitter/GreatRiverMed
>
>
> Information in this communication, including attachments, is confidential
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[Histonet] HIF-1alpha and EBNA1

[Victoria Baker via Histonet]

To All,

I am seaching for a lab that can run both of these antibodies on human FFPE
tissue.

HIF-1 alpha is Hypoxia Inducible 1 alpha
EBNA1 is Epstein Barr Nuclear Antigen 1

If anyone performs these tests or knows of a lab that does, please contact
me.

Thank you in advance

Vikki
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Re: [Histonet] HT Position in Alaska

[Victoria Baker via Histonet]

Me as well.  It's beautiful there!

On Wed, Jun 6, 2018, 12:27 PM Colleen Forster via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> This sounds wonderful..I wish I were in a position to swoop it up!
>
> Colleen Forster HT(ASCP)QIHC
>
> On Wed, Jun 6, 2018 at 9:24 AM, Arrington, Karla A via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Hello All,
> >
> > I hope everyone is enjoying the early part of summer.
> >
> > In beautiful Alaska, our hospital caters to the Alaskan Native population
> > with their entire medical needs being met.
> > Currently we have a Histology Technician position available in our
> > pathology department. This position is M-F, no weekends and a rotating
> > on-call schedule.
> > This position comes with excellent benefits and a team work environment.
> > This also offers relocation assistance for the correct candidate. Must be
> > ASCP certified or eligible.
> >
> > For immediate consideration, email or FAX a current resume to
> > kaarring...@anthc.org and it will be
> > forwarded to our hiring manager. Also visit our website at anthc.org and
> > select Careers to apply online.
> >
> > If you have questions regarding this position or how to submit an online
> > application, please call ANTHC Human Resources at (907) 729-1301 or email
> > care...@anthc.org.
> >
> >
> > Karla Arrington, HT(ASCP) HIT(AHIMA)
> > Pathology Supervisor
> > Alaska Native Medical Center
> > 4315 Diplomacy Drive
> > Anchorage, AK 99508
> > Tele: 907-729-1810
> > Fax:  907-729-1226
> >
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> >
>
>
>
> --
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> B173 PWB  612-626-1930
>
> *If submitting histology request please also forward to Lori Holm at
> ho...@umn.edu *
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[Histonet] Epic and Cerner users, looking for feedback on these systems

[Victoria Baker via Histonet]

Happy Friday!

Our hospital is starting to look at new HIS vendors.  The two that are
being strongly looked at are Cerner and Epic.

Of course the hospital is using a consulting firm who have sort of tied our
hands in terms of following up with vendors and any after the demo
questions we are to filter through them.  We have been told though, by one
of the hospital directors that we can reach out to colleagues and questions.
Currently we have SoftPath DX.  The system is labor intensive and the
version we have is not in keeping with our needs.  But it has taken us
further than we were before in terms of being able to track cases and
monitor our workflow.  We are paired in the hospital with several different
systems such as NexGen and Sorian for financials.  There is also Oracle
database which causes some headaches as well.
I would like to get an idea of what people think that use either of these
systems.  Do they slow down work flow?
  Is it generally user friendly?
 Can you track all entries and produce accurate statistics?
 Are either of these companies strong in support before, during and after
GO LIVE?
Can you incorporate PDF files into reports or access information from the
HIS into the LIS  with little or no hand to computer combat?

These are just surface type questions, but I would truly appreciate
anyone's input.

Thank you and have a nice weekend.

Vikki Baker
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Re: [Histonet] Ann Preece Manual

[Victoria Baker via Histonet]

We currently have a student in our lab.  She took her classes through
Broome community college were the program is in its infancy.  I think she
would benefit from this book.

I can give you contact information off line and it would be a hospital
address.

Vikki Baker
UHS Wilson Hospital


On Oct 28, 2017 6:31 PM, "Victor via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> I have a copy, 1st edition of A Manual for Histologic Technicians by Ann
> Preece. On the inside cover it says Wyoming Valley Hospital Laboratory
> 1962. I don’t recall how I got it, but I never worked for the facility.
> Great for a new student or Histology program. I’ll pay shipping to whomever
> is interested.
>
> Victor
>
> Sent from Mail for Windows 10
>
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Re: [Histonet] Ann Preece Manual

[Victoria Baker via Histonet]

We currently have a student in our lab.  She took her classes through
Broome community college were the program is in its infancy.  I think she
would benefit from this book.

I can give you contact information off line and it would be a hospital
address.

Vikki Baker
UHS Wilson Hospital


On Oct 28, 2017 6:31 PM, "Victor via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> I have a copy, 1st edition of A Manual for Histologic Technicians by Ann
> Preece. On the inside cover it says Wyoming Valley Hospital Laboratory
> 1962. I don’t recall how I got it, but I never worked for the facility.
> Great for a new student or Histology program. I’ll pay shipping to whomever
> is interested.
>
> Victor
>
> Sent from Mail for Windows 10
>
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Re: [Histonet] IHC counterstain offline

[Victoria Baker via Histonet]

Curt,

I am not sure how much you will actually save if you factor in the cost of
a person hand counter staining these slides.  I will be the first to say
Ventana is over priced, but off line counter staining brings in an
additional variable and cost.  In the end is it a real savings?  It's an
individual call, but I wouldn't do it.

Vikki

On Oct 12, 2017 4:43 PM, "Curt via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> We currently use the benchmark ultra and are, in an effort to save a
> little money, looking at running out counter stain offline. Anyone have any
> suggestions or thoughts they might offer? We currently run our H with
> Richard Allen Hematoxylin 7111. I am curious if that is suitable for use in
> your experience or if you recommend a different Hematoxylin for IHC counter
> stains. How much bluing time, etc.
>
>
>
> Thanks,
>
> Curt
>
>
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Re: [Histonet] on call autopsy services

[Victoria Baker via Histonet]

Have  you looked at an ad locum pathologist?  Or a retired pathologist that
would be willing to work as a per diem?

The county coroner might even be willing to get you information or know of
a pathologist willing to come in.  But do you have a dinner or a
Histologist willing to do the assisting?  There are also funeral or
certified morticians who can do assisting.

I apologise for any misspelled words.  I haven't had my tea and my phone
thinks it's a spelling scholar.
Vikki

On Aug 17, 2017 6:53 AM, "Eck, Allison via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Good morning Histonet,
> I am not sure if anyone can help me with this question, but I was asked to
> look into the possibility of finding a pathologist who would be willing to
> come in and perform our autopsies.  We do only few each year so we are not
> looking to hire someone full time but are exploring the option of
> performing the autopsies in house.  Can anyone point me in a direction to
> start?
>
> Thanks in advance
> Allison
>
> Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT)
> Lead Tech Histology
> Doylestown Hospital
> 595 W State St
> Doylestown, PA 18901
> 215-345-2264
> a...@dh.org
>
>
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[Histonet] For NYS laboratories

[Victoria Baker via Histonet]

I'm trying to reach out to other NYS Histology labs.  I can't really get a
real answer regarding a non-CLT doing the embedding/microtomy and staining
of just Autopsy material.  I think that they can't but the actual
documentation is not completely clear.

What are other labs doing?

Thank you in advance for any information.
Vikki
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Re: [Histonet] Stand Alone Heat Extractor

[Victoria Baker via Histonet]

ThermoFisher.  It is on there website.   I don't have the catalog number
but I just ordered one

On May 15, 2017 12:48 PM, "Amanda Reichard via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Everyone
>
> I am hoping someone can help me out here...  I am in search of stand-alone
> heat extractors for our cryostat.  I am told they are hard to come by and
> was curious if anyone knew of any companies that have them?  The one I know
> of isn't an approved vendor yet, and I am trying to avoid going through
> that process.
>
> Thanks in advance!
>
> Amanda Reichard, HTL (ASCP)cm
> Histology/Cytology Supervisor
> Licking Memorial Health Systems
> 1320 W. Main St.
> Newark, OH 43055
> (220) 564-4163
> areich...@lmhealth.org
>
>
> 
>
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Re: [Histonet] Great input on recycled reagents

[Victoria Baker via Histonet]

By using a hygrometer (did I spell that right?) you should be able to see
the purity of the alcohol.  We usually get between 97 to 98 percent.  We do
use fresh absolute alcohol always in the processor.  We start with 80, 95
all recycled.

On Feb 22, 2017 4:55 PM, "Gareth Davis via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Thank you so much.  I will start using them on the processor, but start
> slowly.  Then I can monitor to make sure no problems occur.  I really
> appreciate all the responses.
> Thanks,
>
> --
> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm
> Yuma Gastroenterology
> Yuma, AZ 85364
> 928-248-5259
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Re: [Histonet] Recycled reagents in VIP processor

[Victoria Baker via Histonet]

We routinely recycle xylene and alcohol on a CBG recycler without issues.

We also run ER PR HER2 breast panel, ISH and routine IHC

Vikki Baker

On Feb 22, 2017 3:37 PM, "Gareth Davis via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Hi,
> I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in
> processors.  I am using a VIP 300, refurbished, and I would rather not use
> recycled reagents in it.  But, during the last CAP inspection they
> suggested I use the recycled to save money.  And now my administration
> wants to cut cost.  Just wanted to know what labs were doing.
> Thanks,
>
>
> --
> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm
> Yuma Gastroenterology
> Yuma, AZ 85364
> 928-248-5259
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[Histonet] Dialyzed iron solution

[Victoria Baker via Histonet]

Hi.

Back when I was in research we did Hale's Colloidal Iron stain.  Making the
dialyzed iron was really the big part and back then we were using a
cellulose tubing that we had from another group.  I've been trying to find
the right type but there is just too many choices.  I've also been trying
to find a company that makes it as well.
If anyone can forward some information on where I could find either of
these, I would be grateful for it.

Have a happy weekend!

Vikki
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[Histonet] Cell Blocks

[Victoria Baker via Histonet]

Hi - Happy Friday!

Is anyone on makeing cellblocks with thromboplasting/plasma on cells fixed
in sodium citrate?  These would be pleural fluids and bronch brushings and
washings.

Thanks in advance.

Vikki
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[Histonet] Thromboplastin for cell blocks

[Victoria Baker via Histonet]

Hi

I am looking for Thromboplastin to make cell blocks.  I can't remember
who we got it from years ago.

Any information is much appreciated.

Vikki

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Re: [Histonet] IHC Weekend Coverage

[Victoria Baker via Histonet]

We have Saturday lab coverage which includes IHC.
Case/slides vary between 8-15/25-85.  This includes multiplex and ISH.

Vikki
On Aug 23, 2015 10:49 AM, Cartun, Richard via Histonet 
histonet@lists.utsouthwestern.edu wrote:

 How many of you working in hospital-based pathology laboratories run IHC
 on weekends?  Thank you.

 Richard

 Richard W. Cartun, MS, PhD
 Director, Histology  Immunopathology
 Director, Biospecimen Collection Programs
 Assistant Director, Anatomic Pathology
 Hartford Hospital
 80 Seymour Street
 Hartford, CT  06102
 (860) 972-1596
 (860) 545-2204 Fax


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Re: [Histonet] Breast fixation

[Victoria Baker via Histonet]

Karen
We take ours off on Sunday.  The tissue processor is set up with 6 hours of
fixation the minimum time.  We have VIP 5's so on Friday the tissue goes on
a delay run that is set to come out of formalin at 12:30 AM Sunday
morning.  The end of the process is at 9 AM Sunday morning.  Just take the
tissue off and let it solidify.   When your next tech comes in they can put
the cassette in the embedding center to heat up again and embed it.  You
will follow your same protocol and record your times accordingly.  It might
mean having to ask someone to come in for the 5 minutes it takes to do this
but it works.  If needed you can adjust your times to fit your schedule for
someone being available to take them off.
I hope this will help.
Vikki
On Jul 29, 2015 11:33 AM, Heckford, Karen - SMMC-SF via Histonet 
histonet@lists.utsouthwestern.edu wrote:

 Good Morning,
 I have a question about breast fixation.   I am in a little bit of a
 pickle with the 6-72 hour rule for the fixation on breast tissue.   Friday
 I am getting 2 breast cases in the afternoon and both will not have the
 required minimum 6 hour formalin fixation for my per diem to cut early
 Saturday morning.   He will not be able to make it in again until Monday
 night.  The tissue will be about 3-4 hours (this includes time on the
 processor)  over the 72 hour maximum.Does anyone have any suggestions
 on what can be done?  We are a one person show here.

 Thanks,

 Karen Heckford HT ASCP CE
 Lead Histology Technician
 St. Mary's Medical Center
 450 Stanyan St.
 San Francisco, Ca. 94117
 415-668-1000 ext. 6167
 karen.heckf...@dignityhealth.org

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