Re: [Histonet] Who can perform high complexity testing

[Walter Benton via Histonet]

Amy,

CLIA 88 Guidelines will answer your questions, but here are some PDFs that may 
helpful as well. Certifications are not listed in the CLIA guidelines, but 
educational achievements (e.g. degrees earned and/or college credits earned in 
specific disciplines may qualify an individual).

http://www.jointcommission.org/assets/1/18/clia_required_personnel_qualifications.pdf

http://www.cap.org/ShowProperty?nodePath=/UCMCon/Contribution%20Folders/DctmContent/education/OnlineCourseContent/2014/TLTM/TLTM_Preinsp_personnelrequirementsbytestingcomplexity.pdf

CFR > Title 42 > Chapter IV > Subchapter G > Part 493 > Subpart M > Section 
493.1489
42 CFR 493.1489 - Standard; Testing personnel qualifications.
eCFR
Authorities (U.S. Code)
What Cites Me
prev | next
§ 493.1489 Standard; Testing personnel qualifications.
Each individual performing high complexity testing must -

(a) Possess a current license issued by the State in which the laboratory is 
located, if such licensing is required; and

(b) Meet one of the following requirements:

(1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric 
medicine licensed to practice medicine, osteopathy, or podiatry in the State in 
which the laboratory is located or have earned a doctoral, master's or 
bachelor's degree in a chemical, physical, biological or clinical laboratory 
science, or medical technology from an accredited institution;

(2)

(i) Have earned an associate degree in a laboratory science, or medical 
laboratory technology from an accredited institution or -

(ii) Have education and training equivalent to that specified in paragraph 
(b)(2)(i) of this section that includes -

(A) At least 60 semester hours, or equivalent, from an accredited institution 
that, at a minimum, include either -

(1) 24 semester hours of medical laboratory technology courses; or

(2) 24 semester hours of science courses that include -

(i) Six semester hours of chemistry;

(ii) Six semester hours of biology; and

(iii) Twelve semester hours of chemistry, biology, or medical laboratory 
technology in any combination; and

(B) Have laboratory training that includes either of the following:

(1) Completion of a clinical laboratory training program approved or accredited 
by the ABHES, the CAHEA, or other organization approved by HHS. (This training 
may be included in the 60 semester hours listed in paragraph (b)(2)(ii)(A) of 
this section.)

(2) At least 3 months documented laboratory training in each specialty in which 
the individual performs high complexity testing.

(3) Have previously qualified or could have qualified as a technologist under § 
493.1491 on or before February 28, 1992;

(4) On or before April 24, 1995 be a high school graduate or equivalent and 
have either -

(i) Graduated from a medical laboratory or clinical laboratory training program 
approved or accredited by ABHES, CAHEA, or other organization approved by HHS; 
or

(ii) Successfully completed an official U.S. military medical laboratory 
procedures training course of at least 50 weeks duration and have held the 
military enlisted occupational specialty of Medical Laboratory Specialist 
(Laboratory Technician);

(5)

(i) Until September 1, 1997 -

(A) Have earned a high school diploma or equivalent; and

(B) Have documentation of training appropriate for the testing performed before 
analyzing patient specimens. Such training must ensure that the individual has -

(1) The skills required for proper specimen collection, including patient 
preparation, if applicable, labeling, handling, preservation or fixation, 
processing or preparation, transportation and storage of specimens;

(2) The skills required for implementing all standard laboratory procedures;

(3) The skills required for performing each test method and for proper 
instrument use;

(4) The skills required for performing preventive maintenance, troubleshooting, 
and calibration procedures related to each test performed;

(5) A working knowledge of reagent stability and storage;

(6) The skills required to implement the quality control policies and 
procedures of the laboratory;

(7) An awareness of the factors that influence test results; and

(8) The skills required to assess and verify the validity of patient test 
results through the evaluation of quality control values before reporting 
patient test results; and

(ii) As of September 1, 1997, be qualified under § 493.1489(b)(1), (b)(2), or 
(b)(4), except for those individuals qualified under paragraph (b)(5)(i) of 
this section who were performing high complexity testing on or before April 24, 
1995;

(6) For blood gas analysis -

(i) Be qualified under § 493.1489(b)(1), (b)(2), (b)(3), (b)(4), or (b)(5);

(ii) Have earned a bachelor's degree in respiratory therapy or cardiovascular 
technology from an accredited institution; or

(iii) Have earned an associate degree related to pulmonary function from an 
accredited institution; or

(7) For histopathology, meet the 

Re: [Histonet] slides/cassettes printers points of view

[Walter Benton via Histonet]

We have the original Slidemates (not the newer AS model) and Printmate. Both 
instruments work well.

The slidemate used a thermal ribbons to print. Certain slide coatings (in the 
area where you write/etch/print) are better suited for thermal print 
technology. Not sure what type of slides you use now, but you consider that in 
your purchase decision. In addition slidemates have a small footprint.

Printmate also has a thermal ribbon and you can print 2D barcodes and other 
pertinent identifiers on the cassettes. Generally speaking the printer works 
well with minimal down time due to failures.

Feel free to reach out if you additional questions.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
410-768-5961 (Lab)
Chesapeakeurology.com
Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.




-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 28, 2017 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slides/cassettes printers points of view

Hello Histonettes!!!
I was wondering if somebody are using PRIMERA cassettes and slides printers 
and/or SLIDEMATE slide printer and PRINTMATE AS cassette printer From Thermo.
I am undecided which one to buy. I really love both. They have a lot of 
similarities in efficiency, price and quality BUT my manager is being hearing 
that primera works with cut corner slides ONLY otherwise will create a lot of 
problems.
Have not hear too much about slidemate yet.
If anybody can share their experiences with those printers that can help me to 
decide, I will appreciate. :)



UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] Section adherence issues (IHC)

[Walter Benton via Histonet]

Thanks. This is helpful information.

-Original Message-
From: Hujet, Matthew [mailto:matthew.hu...@ssmhealth.com]
Sent: Wednesday, July 12, 2017 1:35 PM
To: Walter Benton ; Greg Dobbin 
Cc: kgevele...@ihis.org; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Section adherence issues (IHC)

We have had really good luck with both the TOMO and Millennia 2000 adhesion 
slides. We initially tried using the TOMO slides for everything (IHC, special 
stains, and H's), but found that there was too much background staining on 
the specials and H's. For IHC, the TOMO slides were great. We then switched 
to the Millennia 2000 for everything, and they have been working really well 
across the board. With regards to just IHC, I personally feel that the TOMO 
slides are better, but if you only want one type of glass in your lab, the 
Millennia 2000 are the way to go.

Matthew Hujet
Histology Technical Specialist
SSM Health St. Mary's Hospital - Madison
700 S. Park St.
Madison, WI 53715

Please note my new email address: matthew.hu...@ssmhealth.com



-Original Message-
From: Walter Benton [mailto:wben...@cua.md]
Sent: Wednesday, July 12, 2017 10:34 AM
To: Greg Dobbin 
Cc: kgevele...@ihis.org; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Section adherence issues (IHC)

Greg,

We are running into a similar issue on the Biocare Nemesis platform and the 
support there suggest humidity to be the issue. Our sections stay adhered to 
the slides through depar, antigen retrieval, but appear to fall off during the 
staining process. It is not consistent. We are going to try some other slides 
and see if the issue persists. I'd be interested to hear your findings and 
resolution.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061




-Original Message-
From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Cc: kgevele...@ihis.org
Subject: [Histonet] Section adherence issues (IHC)

Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here 
for each other when needed!

My problem is section adherence during IHC staining. And it is not all of the 
time it is intermittent; so not all of the time and not on all slides when it 
does occur.

Background:
We have a Bond-III immunostainer and we use the Leica Apex charged slides for 
our IHC stains. We have no additives in our water baths. Our sections drain in 
a stand and then any trapped water under the section is either flicked or 
wicked away as needed prior to placing the slides in a rack in a
60 C oven for 30 mins prior to staining.  We do not do on board baking 
(primarily because on board baking is only 10 mins long and with the section 
lifting issue we want longer).

Some of the specimen types are more susceptible it would seem. Cervix LEEP 
specimens tend to be bad, we use a 4mm punch to obtain some of our control 
tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad 
one and sometimes our ER/PR control sections (but not always). Fine needle 
cores where a lot of tumour is present tend to be bad (again not always).

We have tried baking longer, we turn ourselves inside out trying to get all of 
the water out from under the sections, we have tried charged slides from 
another manufacturer. I have looked at the HIER protocols and none are 
extraordinary in nature. We use very clean covertiles (no scratches or 
blemishes). Our specimens are fixed for 24hrs before processing. We use the 
same slides for Special Stains and don't have this issue there.

I need some new suggestions to try! All ideas welcome.
Cheers,
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!* 
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Re: [Histonet] Section adherence issues (IHC)

[Walter Benton via Histonet]

Greg,

We are running into a similar issue on the Biocare Nemesis platform and the 
support there suggest humidity to be the issue. Our sections stay adhered to 
the slides through depar, antigen retrieval, but appear to fall off during the 
staining process. It is not consistent. We are going to try some other slides 
and see if the issue persists. I'd be interested to hear your findings and 
resolution.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061




-Original Message-
From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Cc: kgevele...@ihis.org
Subject: [Histonet] Section adherence issues (IHC)

Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here 
for each other when needed!

My problem is section adherence during IHC staining. And it is not all of the 
time it is intermittent; so not all of the time and not on all slides when it 
does occur.

Background:
We have a Bond-III immunostainer and we use the Leica Apex charged slides for 
our IHC stains. We have no additives in our water baths. Our sections drain in 
a stand and then any trapped water under the section is either flicked or 
wicked away as needed prior to placing the slides in a rack in a
60 C oven for 30 mins prior to staining.  We do not do on board baking 
(primarily because on board baking is only 10 mins long and with the section 
lifting issue we want longer).

Some of the specimen types are more susceptible it would seem. Cervix LEEP 
specimens tend to be bad, we use a 4mm punch to obtain some of our control 
tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad 
one and sometimes our ER/PR control sections (but not always). Fine needle 
cores where a lot of tumour is present tend to be bad (again not always).

We have tried baking longer, we turn ourselves inside out trying to get all of 
the water out from under the sections, we have tried charged slides from 
another manufacturer. I have looked at the HIER protocols and none are 
extraordinary in nature. We use very clean covertiles (no scratches or 
blemishes). Our specimens are fixed for 24hrs before processing. We use the 
same slides for Special Stains and don't have this issue there.

I need some new suggestions to try! All ideas welcome.
Cheers,
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
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Re: [Histonet] scrubs/business casual

[Walter Benton via Histonet]

Bill,

It is a required uniform for our staff. First three sets are provided upon 
hire. All additional scrubs are purchased by the employee.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates



-Original Message-
From: O'Donnell, Bill via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 21, 2017 9:36 AM
To: Histonet (histonet@lists.utsouthwestern.edu) 

Subject: [Histonet] scrubs/business casual

Just a little poll.

How many histology labs allow scrubs?

Of those that are allowed scrubs, do you purchase your own, or are they 
provided?

Bill O'Donnell
Good Sameritan Hospital
Histology

This email and attachments contain information that may be confidential or 
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Re: [Histonet] Microwave processors

[Walter Benton via Histonet]

Charles,

Milestone Medical http://www.milestonemed.com/

The processors are great. Easy to operate and use minimal chemicals based on 
the protocols you select.  H and IHC are very good.

Check out the various product offerings to see which unit will meet your needs 
best.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 14, 2017 9:39 AM
To: Histo List 
Subject: [Histonet] Microwave processors

Does anyone have any suggestions for microwave processing units? My lab 
processes about 110 blocks max a day from GI biopsies to breast tissue on size

Sent from my iPhone
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Re: [Histonet] CAP code for H

[Walter Benton via Histonet]

Hope this helps.

ANP.10042 Histologic Prep Quality Phase I
There is a written procedure that describes the process by which pathologists 
or their
designees provide feedback to the histology laboratory on the quality of 
histologic
preparations. This procedure must include the daily recording of the quality of 
the
histologic preparations for each day of tissue processing and slide preparation.
NOTE: Histologic preparations refer to H & E sections, histochemical stains,
immunohistochemistry preparations, and in situ hybridization preparations.
This requirement applies to laboratories that process and interpret histologic 
preparations at the
same location, as well as laboratories that interpret histologic preparations 
processed at another
laboratory (regardless of that outside laboratory's accrediting organization).
Records of such feedback and corrective action taken when problems are 
identified may be
incorporated into the laboratory's quality management program.
Specific quality control requirements for special stains, immunohistochemistry, 
and other special
studies are found elsewhere in this checklist.
Evidence of Compliance:
✓ Records of feedback and corrective action for problems identified with 
histologic prep quality

ANP.11734 Slide Quality Phase II
Slides are of sufficient quality for diagnosis.
NOTE: Histopathology slides must be of adequate technical quality to be 
diagnostically useful.
Criteria to evaluate include adequate tissue fixation, processing, thickness of 
sections, absence
of interfering tissue folds and tears, and good staining technique and cover 
slipping. For
hematoxylin and eosin and other routine stains, the patient slide serves as the 
internal control to
ensure adequate staining technique. The sections must be cut from sufficient 
depth in the block
to include the entire tissue plane.

**REVISED** 07/28/2015
CYP.04300 Daily QC Phase II
There are records of daily review of the technical quality of cytologic 
preparations by the
pathologist or supervisory-level cytotechnologist.
NOTE: The technical quality of cytologic preparations must be checked daily (on 
days processing
occurs). This includes checking all stains for predicted staining 
characteristics each day of use.
This check must include all of the types of preparations seen that day such as 
cytospins, cell
blocks, and liquid based preparations.
If preparation and staining is performed by a different laboratory, there must 
be a procedure for
the laboratory performing the preparation and staining to verify the 
acceptability of the quality
of preparations and the acceptability of controls (if needed) before transfer. 
Records of this
verification must be readily available to the laboratory performing 
interpretations. There should
also be a mechanism for feedback from the interpreting laboratory to the 
laboratory that prepared
the slides of any issues with the preparations.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.


-Original Message-
From: Nina J. Rich via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, April 24, 2017 11:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP code for H

Does anyone know if CAP requires that labs run an H control daily? If so what 
is the # for that requirement?  Also, for pap stains?

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Re: [Histonet] looking for a slider printer

[Walter Benton via Histonet]

http://accuplace.com/products/pslim/

Manufacturers of the original slidemate (Thermo Fisher).

Solid instruments with minimal fuss. Keep them clean and maintained according 
to manufacturer specs and they work well.

Using proper Thermal Slides are also important for getting a good print and not 
destroying the print head.

Feel free to reach out if you have additional questions.

Walter Benton HT(ASCP)QIHC


-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, April 21, 2017 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] looking for a slider printer

Dear Histonets!!!
We are looking to buy a slide printer for a low cost, durable and easy to work. 
W are a very small lab, we don't' need anything fancy. If you know somebody or 
have a good experience with one please let me know.


Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] Survey!!!!!!

[Walter Benton via Histonet]

​Patti,


If you are able to incorporate the special stains along with the H on the 
automated stainer, I think that would be the best option. Based on your 
information you may have 120-180 slides to coverslip by hand each day.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant 
Sent: Friday, March 31, 2017 3:50 PM
To: Walter Benton; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Survey!!

Walter the staff consist of 1 Histotech and 1 lab assistant. On an average 
there will be 2 special stains per block. Hope that helps.

Sincerely,

PATTI NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.


-Original Message-
From: Walter Benton 
To: Patti Nelson - PNP Lab Consultant ; Histonet 

Sent: Fri, Mar 31, 2017 12:39 pm
Subject: Re: [Histonet] Survey!!

How many slides are you producing from that number of blocks? How many techs do 
you have that are able to hand stain and/or hand coverslip?

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant via Histonet 
>
Sent: Friday, March 31, 2017 2:58 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Survey!!

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated.



Sincerely,

PATTI NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law. Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful. If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call 909-841-9761.

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Re: [Histonet] Survey!!!!!!

[Walter Benton via Histonet]

How many slides are you producing from that number of blocks? How many techs do 
you have that are able to hand stain and/or hand coverslip?

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant via Histonet 

Sent: Friday, March 31, 2017 2:58 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Survey!!

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated.



Sincerely,

PATTI NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.

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Re: [Histonet] Newbe help

[Walter Benton via Histonet]

Start with this link and navigate through the website. There are various online 
and in-person programs. Best of luck.
http://nsh.org/content/schools


-Original Message-
From: Keith Brown via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, March 06, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Newbe help

Hi.
I've looked through the archives of this group to little avail. If i missed an 
answer to my questions that was previously posted to this list, my apologies.

I'm looking to get into the histotech field, but I'm not sure how to go about 
it. I'm changing careers after 20 years as a journalist. I have an associates 
and a bachelor's degree, neither of which are in anything remotely science-y: 
Liberal Arts and Journalism, respectively.

I live in New Jersey, where there does not appear to be any histology training 
programs, yet most of the jobs I see posted throughout the state want a 
certification.

I have a friend in Seattle who fell into histology after journalism, but he 
admits he got lucky and still does not have a certification. He gets paid 
slightly less, he says, but not by much. It sounds like a good career choice 
for me, but I'm not sure how to get it started.

Any and all advice is appreciated.

Thanks,

Keith.
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Re: [Histonet] hand staining immunos

[Walter Benton via Histonet]

http://www.agilent.com/cs/library/technicaloverviews/public/08002_ihc_staining_methods.pdf


-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 09, 2017 10:51 AM
To: Jennifer ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] hand staining immunos

You do not have to "babysit" the procedure for it has well defined/timed steps. 
You just need a timer and check the slides when required. As to procedural "dos 
& donts" try to get a copy of the DAKO IHC manual.René

On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet 
 wrote:


 Hello,



My dermatopathologist wants me to start hand staining immunos  for MITF. I 
would really like some feedback about everyone's experiences/thoughts with 
manually hand staining immunos and especially with MITF. Pros and cons? I am 
concerned about the amount of time this will take for a tech to babysit the 
stain and also about the consistency of the quality in the end result.  Your 
opinions are much appreciated.



Thank you!



Jennifer

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Re: [Histonet] Syringe for Lab Vision 720D Stainer Needed!

[Walter Benton via Histonet]

Check Biocare Medical. They use the same "box" and just place their name on it. 
Biocare calls it the Nemesis.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Sandra Cheasty via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, January 30, 2017 4:01 PM
To: Histonet (histonet@lists.utsouthwestern.edu) 

Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed!

Hello everyone!
Can someone tell me where I can get a replacement syringe for 
the Lab Vision 720 stainer? It is supposed to be part # NM014. Thermo Fisher 
has been unable to help me so far.
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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Re: [Histonet] Slide and Block Storage

[Walter Benton via Histonet]

Depending on the contract that you select with your vendor, you can determine a 
cutoff time for same day deliveries. We order by noon and have the delivery by 
3pm the same day. Orders placed after 12pm are delivered on the next business 
morning.

I'm sure there are more rapid options, but they are probably more costly as 
well.

-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, January 12, 2017 10:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and Block Storage

Happy Thursday connected to Friday,

Have a few questions about slide and block storage...


Are you blocks and slides stored on-site or off-site at a record control type 
facility?

If your blocks and slides are stored off-site how do you get the material that 
you need at any given point of the day?



Thanks in advance for your help,
Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org

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Re: [Histonet] CAP checklist help

[Walter Benton via Histonet]

Leica sells a temperature verification kit that should meet your needs for CAP 
compliance and documentation. We use it for our Leica Thermobrites used in the 
processing of FISH.

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, December 28, 2016 11:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP checklist help

I need help with figuring out how to meet checklist # ANP.23420.   We use
the Leica Bond III and MAX  to do our ISH slides.  How do I test the 
temperature and how would you recommend recording the results?

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] Temp Assignment for Chesapeake Urology

[Walter Benton via Histonet]

To all:

Our lab is in search of temp help to cover an extended absence by a staff 
member.

The ideal candidate will have Prostate Grossing experience and be eligible to 
gross based on CLIA 88 Guidelines. In addition, this person should be a strong 
prostate biopsy cutter and embedder. The assignment will be 4 weeks in length. 
This will be a 40hr/week assignment. The shift may start as early as 4 am or 
6am depending on the individuals skills and abilities to meet the needs of the 
lab.

If interested, please send your resume along with desired salary to:
mhw...@cua.md and myself 
wben...@cua.md


From: Walter Benton
Sent: Monday, December 19, 2016 12:07 PM
To: histonet@lists.utsouthwestern.edu
Cc: Michael Hwang 
Subject: Temp Assignment for Chesapeake Urology

To all:

Our lab is in search of temp help to cover an extended absence by a staff 
member.

The ideal candidate will have Prostate Grossing experience and be eligible to 
gross based on CLIA 88 Guidelines. In addition, this person should be a strong 
prostate biopsy cutter and embedder. The assignment will be 4 weeks in length. 
This will be a 40hr/week assignment. The shift may start as early as 4 am or 
6am depending on the individuals skills and abilities to meet the needs of the 
lab.

If interested, please send your resume along with desired salary to:
mhw...@cua.md and myself 
wben...@cua.md

Thanks

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

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Re: [Histonet] specimen submission pads

[Walter Benton via Histonet]

Biopsy wraps or 
http://www.amazon.com/Graham-Jumbo-Papers-Perms-1000ct/dp/B001J9R93W

Either of these items can be wrapped to form an envelope preventing the tissue 
from floating around.

-Original Message-
From: Linda Margraf via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 06, 2016 12:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] specimen submission pads

Hi Histonetters,
We are having trouble in the Gross room,  removing small specimens from the 
pads/gauze that OR personnel put them on which they then immerse in formalin.  
We thought the gauze was challenging to remove tiny specimens from but have 
found that the Telfa pads the OR is now using come apart in the fixative and 
are even more challenging to work with. The GI lab uses sponges but they would 
be too small for the usual size containers the OR send us.  Any suggestions?

Thanks in advance,
Linda M
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[Histonet] Brain IHC

[Walter Benton via Histonet]

Please see message below. Feel free to reach out directly to the Pathologist.

From: Rudolph J Castellani [mailto:rudolph.castell...@med.wmich.edu]
Sent: Friday, March 25, 2016 8:55 AM
To: Walter Benton 
Subject:


Another question - is there any option of embedding big brain slices in 
paraffin and doing 50 micron IHC on them, or is that not possible?


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Re: [Histonet] Sliding Microtome

[Walter Benton via Histonet]

Thanks to those who have responded.

Anyone able to share or train on techniques for free floating IHC? He needs 
that skill set too.

-Original Message-
From: Walter Benton via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, March 24, 2016 8:45 PM
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Fw: Sliding Microtome

(2nd attempt)?

To all:



I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Walter Benton
Sent: Thursday, March 24, 2016 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: Sliding Microtome

To all:

I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com<https://www.cua.md/owa/chesapeakeurology.com>

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
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[Histonet] Fw: Sliding Microtome

[Walter Benton via Histonet]

(2nd attempt)?

To all:



I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Walter Benton
Sent: Thursday, March 24, 2016 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: Sliding Microtome

To all:

I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
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Re: [Histonet] Plastic coverslip Loosening

[Walter Benton via Histonet]

This may work. I have used a version of this product on glass coverslips and 
slides to transfer paraffin sections.

http://www.newcomersupply.com/product/mount-quick


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.




-Original Message-
From: Helen Fedor via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, March 22, 2016 9:45 AM
To: HISTONET (histonet@lists.utsouthwestern.edu) 

Subject: [Histonet] Plastic coverslip Loosening

Hello, I did try and search the archives to see if there was any advice on this 
but didn't find an answer.

Do you know of a good way to fix H slides that had plastic coverslips where 
the coverslip is lifting from the slide and in some cases coming completely 
off? In all cases the tissue is on the coverslip.

Thanks in advance.



Helen L. Fedor


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Re: [Histonet] Microscopic slides

[Walter Benton via Histonet]

Try Globe and Tanner slides, both should work well in your slidemate.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Vickroy, James via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, March 07, 2016 1:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microscopic slides


We are once again reviewing the microscopic slides we use.  We have found the 
sldies we have used on a regular basis take too long to dry properly which 
gives us some undesireable artifacts.  I have longed for a "one size fits all" 
microscopic slide.One of the slides I used in the past is not possible 
given that I have the Thermofisher Slidemates.   The slidemates require a very 
smooth labeled end.   Recently we tested several slides both hydrophobic and 
hydrophilic.  We really liked a hydrophilic slide by Leica but am told it 
doesn't work well for IHC's and special stains.   Our current slide is a 
"hydrophobic" slide which traps water easily underneath the section and 
therefore we having drying problems.  If we don't dry them for at least twenty 
minutes we get nuclear bubbling.  Leica has another slide we tried that is 
"hydrophobic" but with "hydrophilic" tendencies.  My staff thought tried that 
slide and found them to be very similar to the "hydrophobic" slide we already 
have.

We have tried the Stat-lab M2000 and they seem to  dry better but there often 
is a "trick" to getting the sections to stick to the slide without sliding off 
of the end.Of course we might just have to use two different slides, one 
for our routine H's and one for the IHC's and Special stains.   Anybody have 
any thoughts on this?

And is a "hydrophobic slide" that has "hydrophilic tendencies" really possible?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
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Re: [Histonet] Giemsa Tissue control options

[Walter Benton via Histonet]

http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MGIEMSA.PDF


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, February 23, 2016 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Giemsa Tissue control options

I need to run a giemsa test and haven't done it in a long time. Is there 
something I can use as a control tissue other than a blood smear or spleen?

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Need Slide Cabinets

[Walter Benton via Histonet]

http://www.phoenixmetalproducts.com/


-Original Message-
From: Rayen Gonzalez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, February 12, 2016 12:05 PM
To: histonet@lists.utsouthwestern.edu
Cc: Lin Bustamante 
Subject: [Histonet] Need Slide Cabinets

Hello all!

Our lab is in need for STACKABLE METAL SLIDE CABINETS. I would like to know 
which vendors supply this product and if you can send me a quote? Or if your 
lab is content with the slide cabinets you use, if you can give me your vendors 
information?


Thank you,


Rayen Gonzalez B.S., HT (ASCP)
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Re: [Histonet] NSH Continue education award

[Walter Benton via Histonet]

http://www.nsh.org/content/scholarships


-Original Message-
From: Bustamante, Lin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 10, 2016 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] NSH Continue education award

I would like to nominate a student I have. Can someone guide me what to do next?
Thank you very much.
Lin.

Lin S. Bustamante B.Sc. H.T.(ASCP)
Research Associate
Texas A University
College of Veterinay Medicine
VIBS Histology Laboratory Supervisor
Room 107 VMA
College Station, Texas 77843-4458
(979)845-3177
(979)458-3499 Fax

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Re: [Histonet] Temperatures

[Walter Benton via Histonet]

Secondary thermometer in paraffin reservoir of  embedding center.

-Original Message-
From: Fawn Bomar via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 03, 2016 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperatures

Hi All,



I was wondering how everyone tracked their cryostat and paraffin temperatures.  
Do you all just use the machine temperature, or do you all use the machine and 
a second thermometer as verification?



Thank you

Fawn
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Re: [Histonet] tissue flotation bath

[Walter Benton via Histonet]

http://www.sakura.eu/Our-products/item/8/Microtomy/31/Sakura-Water-Bath-Hot-Plate/Downloads

It appears to have a light, but can't tell for sure.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, February 02, 2016 11:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue flotation bath

Hello everyone,

I have a Surgipath flotation bath that I love. It is square, black inside, 
lighted and no glass liner. The things heats up in 5 minutes!

I know Surgipath is no longer, but I thought I found a similar product online a 
year or so ago but now cannot find anything like it. (Except for 1 but has no 
light)

Can anyone direct me? And vendors are more than welcome to reply.

Thank you,

Jeanine H. Sanders, BS, HT (ASCP), QIHC
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS/G-32
Atlanta, GA 30329
j...@cdc.gov
404-639-3590

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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

Histoscreen cassettes will work as well. Generally the cassette options are 
expensive and may not work in all cassette printers, if you are using one.

http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscreen-cassettes.html

Ultimately, get samples of whatever you like to use.

From: Caroline Miller [mailto:mi...@3scan.com]
Sent: Friday, January 29, 2016 1:36 PM
To: Walter Benton <wben...@cua.md>
Cc: Charles Riley <cri...@dpspa.com>; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processing question

I really like this type:
https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584
(although I buy them from mastertech, but they seem to have dissapeared from 
their website)
They are great for both large tissues, and also biopsies. A long time ago when 
I worked in a clinical lab we used the tissue paper and I found that if 
everything was not heated just right the biopsies would stick and things like 
currettes were hard to scrape up from there, I always thought I was doing the 
tissue damage
yours
mills

On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet 
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet 
[mailto:histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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without making a copy.

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--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Disposal of blocks and slides

[Walter Benton via Histonet]

Charles,

Not sure if you are CAP accredited or will be, but this is from our checklist 
for the last inspection period.

ANP.12500 Record Retention Phase II
Surgical pathology records and materials are retained for an appropriate period.
NOTE 1: Minimum requirements for surgical pathology, providing these are not 
less stringent
than local, state and national regulations, are:
1. Accession log records - 2 years
2. Wet tissue (stock bottle) - 2 weeks after final report
3. Paraffin blocks - 10 years (subject to Notes 2 and 3, below)
4. Glass slides (including control slides) and reports - 10 years (slides must 
remain
readable for this period)
5. Surgical pathology reports - 10 years (see Notes 4 and 5, below)
6. Fluorochrome-stained slides - at the discretion of the laboratory director
7. Fine needle aspiration slides - 10 years
8. Images of FISH studies - 10 years (see Note 6, below)
There must be a documented policy for protecting and preserving the integrity 
and retrieval
of surgical pathology materials and records. The retention period should be 
extended, when
appropriate, to provide documentation for adequate quality control and medical 
care.

NOTE 2: Regarding extra-institutional release of blocks for research purposes: 
Federal
regulations require that a laboratory retain paraffin blocks for two years 
unless the tissue is
blocked specifically for research and not used for patient diagnostic 
purposes.* The CAP
Commission on Laboratory Accreditation (CLA) requires, however, that paraffin 
blocks used for
patient diagnostic purposes must be kept for at least 10 years. Nevertheless, 
such blocks may
be released for research purposes after the two-year regulatory requirement if 
all of the following
criteria are met:
1. For laboratories subject to US regulations, formal written authorization is 
obtained
in accordance with the requirements of HIPAA if identifiable patient 
information is
released.
2. The laboratory retains sufficient blocks to support the diagnosis for the 
full 10-year
period.
3. Provision is made for retrieval by the laboratory of any blocks or material 
that remain
after use in research, if the blocks or material are needed for diagnostic, 
legal, or
other legitimate purposes.
4. The laboratory meets other relevant requirements including but not limited 
to the
requirements of the institution, the directives of any applicable institutional 
review
board (IRB) or similar entity; and state and local laws and regulations.
NOTE 3: Given that patient survival rates are increasing and the continued 
emergence of
treatment based on biomarker testing, which at times may be required on the 
original tissue, it
is recommended that, whenever feasible, tissue block retention from patients 
with diagnosed
malignancies be retained beyond the 10 year requirement.
NOTE 4: Pathology reports may be retained in either paper or electronic format. 
If retained
in electronic format alone, however, the electronic reports must include a 
secure pathologist
electronic signature. Images of paper reports--such as microfiche or PDF 
files--are acceptable.
NOTE 5: Reports of outside consultations performed on cases from the laboratory 
(whether or
not such consultation was requested by the laboratory) must be retained for 10 
years after the
date on which the original report was issued.
NOTE 6: There is no retention requirement for images when the source slides 
remain readable
for the required 10-year retention period. The 10-year retention requirement 
applies to images of
slide preparations that are not readable for the 10-year period (e.g. FISH 
studies).
*The restriction on release of blocks does not prohibit release of blocks for 
purposes of
treatment, diagnosis, prognosis, etc., for patients on research protocols as 
long as release
is consistent with patient privacy regulations (e.g. HIPAA) and applicable 
state and local


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 12, 2016 8:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Disposal of blocks and slides

Hello all,

 What are the guidelines for disposal of blocks and slides? This was never 
discussed in my program and I am now in charge of the department. No one who 
currently works here has been through the process. Any help will greatly be 
appreciated.

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] NSH Financial and Tax Statements

[Walter Benton via Histonet]

The website has some of the information you are speaking about, but I don't 
think tax statements are usually provided. They have the financials there for 
your review and I have included the link as well.

http://www.nsh.org/sites/default/files/2014%20Annual%20Report%20Final.pdf


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: WILLIAM DESALVO via Histonet 
Sent: Thursday, January 7, 2016 4:31 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] NSH Financial and Tax Statements

As a due paying member of NSH, I ask all members and anyone interested in 
Histotechnology request from NSH office to see the Financial and Tax Statements 
for 2013 / 2014. This is public information. We should all know where our dues 
money, money from educational activities and vendor money from the convention 
is being spent.

Sent from my Windows Phone
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[Histonet] Lab Manger Position

[Walter Benton via Histonet]

Hello, thanks Walter for your assistance.

We slightly modified our posting. Here is the updated version. For more 
information about our lab, feel free to visit our 
website.

Please contact one of the two Pathologists listed below if you are interested 
in more information -
adnan.k...@icmdiagnostics.com  (Medical 
Director)
tom.hueb...@icmdiagnostics.com (Chief 
Pathologist)

Summary Description:
Directs and controls the day-to-day Pathology/Histology department operations 
including personnel activities, technical oversight and work flow.

Essential Functions:
* Manages all histology activities, including recruiting, hiring and training 
of staff.
* Ensures competency, motivates and encourages professional growth.
* Controls costs by monitoring productivity, personnel utilization, overtime, 
material usage rates, analyzing fluctuations in types and volumes of tests, and 
implements corrective actions.
* Ensures compliance with regulatory and accreditation agencies' rules and 
regulations.
* Designs and implements effective risk control processes.
* Researches and resolves client problem/issues.
* Oversees and coordinates startup and implementation activities resulting from 
new services or transfer of services.
* Participates in department, inter-department, inter-facility, and 
inter-regional level projects which help the regional laboratory achieve its 
goal of providing quality service and client support in a cost effective manner.
* Develops transition plan for new services/tests, outreach programs for 
transfer of work, timelines, and monitors milestones to achieve service 
expectations.
* Coordinates internal resources to support new service.
* Develops needs analysis as appropriate.

Basic Qualifications:
Experience
* Minimum five (5) years of experience in high-volume histology laboratory 
required.
* Minimum three (3) years of supervisory/managerial experience.
Education
* Bachelor's degree in biological sciences or related field required.
License, Certification, Registration
* Certification by the American Society for Clinical Pathologists.

Location:
Greenbelt, Maryland

Thank you,
Adnan


Adnan R. Khan, MD
Medical Director
Integrated Cellular & Molecular Diagnostics
P: 844.522.4263 | F: 301.363.1099 | C: 
703.282.1796
adnan.k...@icmdiagnostics.com
www.icmdiagnostics.com


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
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Re: [Histonet] Patient identifiers

[Walter Benton via Histonet]

Full name and date of birth.

-Original Message-
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, September 15, 2015 10:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Patient identifiers

What are people accepting for the "two" identifiers on the small formalin 
bottles that are used for prostate biopsy specimens?  Can you accept the 
patient's initials in place of the full name?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] Automatic coverslippers

[Walter Benton via Histonet]

Jessica,

I have the same Leica stainer with the attached coverslipper. CV 5030. It uses 
glass coverslips in varying sizes (you choose) based on what works for your 
lab. If you have a large volume of work to coverslip each day this will 
definitely save you time. More importantly, the coverslipping will not vary, as 
it can from tech to tech. The slides can be dry in 24-48 hours completely, but 
that is determined more by the mountant type and volume being applied. Archival 
is fine with no problems since the glass system is not as particular as the 
tape from other vendors. ***Tape is faster though.***

As for common issues that you may experience if the unit is not cleaned and 
maintained properly. Broken coverglass can cause jams or even worse break 
slides. The mountant needle can bend if the brush is allowed to dry and becomes 
hard, which can then cause your slides to receive little to no mountant on 
them. The arm that picks up slides can get out alignment, but this can easily 
be fixed with a restart (re-initialization) of the coverslipper.

As for removing the left facing side of the unit, I think that can be done to 
retrofit the instrument with the link system, but check with your Leica rep to 
confirm. Linking the two systems removes the need for constant interaction by 
techs loading and unloading racks throughout the day.

The take home message to all of this is, keep it clean and it generally works 
great every day without issue. If you don't it will eventually cause you havoc.

We are very happy with our unit.

If you have any other questions, please don't hesitate to reach out to me.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

-Original Message-
From: Piche, Jessica via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, September 09, 2015 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automatic coverslippers

Hey Everyone,

I've been tasked to collect information on automatic coverslippersI was 
wondering what everyone else is using out there? Do you like them? Do they make 
your lives easier or more difficult? How do the slides hold up over time? We 
have a Leica Autostainer XL. Can it be retrofitted for an Autostainer?

Thanks in advance>:)

Jessica Piche, HT(ASCP)
Waterbury Hospital



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