Re: [Histonet] RE: Paraffin Temperature Checks
I like to use a chart recorder on paraffin dispensers in case the thing goes whacko over a weekend and boils the paraffin, then goes back to normal before anyone notices. Sent from my iPhone On Jan 8, 2015, at 6:10 PM, Elizabeth Chlipala l...@premierlab.com wrote: All of our logs are use logs so we record when we use any piece of equipment and any QC or maintenance if required, we do not record or document anything if we do not use it, other than lab temperature and humidity which we record daily. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, January 08, 2015 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Temperature Checks For those of you out there that are fortunate enough to have tissue processors or embedding centers that are used less often than daily-like maybe even weekly (or even less frequently,) how often are you checking and documenting the paraffin temperatures on said pieces of equipment? For our daily equipment, it's no big deal obviously. But we have a research processor and embedding center that doesn't get used often-sometimes for a week or two, and if we're not touching the machine, it seems overkill to document paraffin temps daily. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Salary exempt/nonexempt status
Are you paid a. by the hour or was your pay quoted to you by b. yearly salary? C. Do you fill out a time card to record your hours? If A and C are yes, you are non exempt. Your human resource department should have the answer. If you don't have an HR dept and you cant ask your boss to clarify, then you have a crummy relationship with your boss, and should look for a better job. Sent from my iPhone On Oct 25, 2014, at 4:38 PM, Sebree Linda A lseb...@uwhealth.org wrote: I fought this very same battle having been salaried for many years and gradually working more and more OT. I worked solely with hourly employees and was not a manager or supervisor. Everyone I worked with accrued OT pay but I did not and I was usually working the most OT. I contacted our state office of labor and workforce development and learned about the exempt/nonexempt statuses. Upon bringing this information to both my institution's personnel department and eventually my manager, they reviewed my position description along with several other employees. They resolved the issue by making me and some other people hourly with no cut in pay so now I still work some overtime but accrue OT pay. I also received back pay from 2 years of overtime. Maybe CA has a labor department that could help you as well. Good luck, Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of GMail [nguy0...@gmail.com] Sent: Saturday, October 25, 2014 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary exempt/nonexempt status Can someone please help me, I've been losing sleep over this ever since moving to CA and working here. If you are paid a salary but NOT a supervisor or manager are you considered exempt or nonexempt? I was told by someone that if you are salary but not in a managerial position you are considered nonexempt and are entitled to earn OT. I have tried searching the laws in CA and the only thing I could find is: 1. If you earn twice the wage of minimum wage you are exempt. 2. If you are a type of professional (doctors and lawyers etc) or in a managerial position you are exempt With that being said, I believe a lot of people in the U.S with other professions make twice the wage of #1, so does that mean they are all exempt? Please help me answer this question! Thank you in advance! Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Preventative Maintenance Fees Gone Wild
Pm or a service contract? Sent from my iPhone On Aug 29, 2014, at 11:58 AM, Sandra Cheasty cheas...@svm.vetmed.wisc.edu wrote: Does anyone else think that $8,266 sounds a bit high for a PM on a Lab Vision Immunostainer? Does that price include a Happy Ending? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Cornflaking artifact
Is this a tape coverslipper? If so, you do have minute traces of water carryover to your xylene. If there is even the hint of pink in you last dehydrant before xylene, you will get that cornflake artifact. Acetone wont help, because water still be present. Increase number of absolute alcohol before xylene, and check often for eosin carryover. This fixed our problem. Jackie O' Sent from my iPhone On Mar 12, 2014, at 21:59, Sharon Scalise sscal...@beaumont.edu wrote: I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Soaking artifact
Your supervisor is wrong, and inexperienced. What artifact? Some tissues MUST be soaked on wet ice, spleen, liver, eye lens, anything bloody - You just can't get quality sections without soaking some tissues. You can tell her I said so. I'd like to know what artifact she is 'seeing'. Jackie O'Connor -Original Message- From: Deanna Leslie dea.les...@gmail.com To: histonet histonet@lists.utsouthwestern.edu Sent: Sun, Jan 5, 2014 3:44 pm Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rodent eye paraffin microtomy
I have a study coming up where obtaining perfect lens histology is critical. We do a pretty good job routinely, but since the lens is of interest, I would appreciate any tricks and techniques that can help us improve our paraffin lens histology within intact rodent eyes. Thanks, Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting issues
My educated guess would be that the tissues are being dried out prior to being placed in formalin at time of collection. Surgeons are our biggest histology problems. Jackie O' -Original Message- From: April P - Assocd apr...@associateddermhelena.com To: histonet histonet@lists.utsouthwestern.edu Sent: Thu, Dec 12, 2013 11:24 am Subject: [Histonet] Cutting issues Need feedback. We are a Derm lab We are having an issue with some small punches and shaves being crushed or arched. It does not happen every day and it is maybe one or two when it is appening. Does anyone have any ideas on what can be causing this issue? ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histology tracking systems for pharma or contract lab
xybion is in the process of developing a histology module for Phama histo. We at AbbVie will be using this system. Jackie Sent from my iPhone On Dec 5, 2013, at 13:23, Kim Merriam kmerriam2...@yahoo.com wrote: Hi, My company is considering a new tracking system for our histology studies. This system needs to track everything from trimming, decal, embedding, cutting, routine and molecular pathology/staining. It also needs to be able to accommodate complex study designs (multiple animals, groups, necropsy timepoints, etc). I am interesting in hearing from people in other pharma/biotech/CROs to see what they are using to capture this information. Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] coverslipping question
Are you using tape or glass coverslips? We have found that when using tape coverslips, at least two very clean 100% alcohol steps must be added before slides go into the clearing reagent, be it xylene or Histoclear. If not, brown spots appear on some tissues, usually liver and bone. If there is any TRACE of water retained in the tissue, it will show up as a brown artifact on the tissue. This doesn't happen with conventional glass coverslipping and mounting medium - I think the mounting medium compensates for the retained moisture in the tissues. Jackie -Original Message- From: Kim Donadio one_angel_sec...@yahoo.com To: Gautier, Nicole M. nga...@lsuhsc.edu Cc: histonet histonet@lists.utsouthwestern.edu Sent: Wed, Nov 13, 2013 1:37 pm Subject: Re: [Histonet] coverslipping question I would try adding 1 or 2 more 100% alcohols before the histoclear. Sent from my iPhone On Nov 12, 2013, at 9:32 AM, Gautier, Nicole M. nga...@lsuhsc.edu wrote: My lab has been having a problem with specks appearing on our slides after they come out of Histoclear. Our protocol is to dehydrate in 2 minutes each of 70%, 80%, 95%, and 100% ethanol before 2 minutes 2 times in Histoclear. The slides are perfectly clear when they come out of the ethanols, but not when they come out of the Histoclear. Since it's not a problem we had when we first started, I tried changing out the Histoclear, but the problem remained. At the end of last week, I changed out everything - all glassware, all ethanols, all Histoclear. The problem went away and the 24 slides looked fine. Today, the slides are looking speckled again. So I guess my questions are: What could be causing the speckled appearance of the slides? and How often should I have to change the ethanols and Histoclear? I seem to remember years ago when I did this that we only changed the solutions monthly... Nicky Gautier Research Associate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] processing eyes
We transfer eyes out of Mod Davidsons after 24 hours and process with the other
soft tissues in formalin- why transfer to alcohol? We have beautiful eye
sections.
Jackie O'
Sent from my iPhone
On Aug 8, 2013, at 3:12 PM, Bea DeBrosse-Serra bdebrosse-se...@isisph.com
wrote:
Patsy,
18-24 hours in Davidson's followed by 70% ethanol prior to processing. Some
people also store the eyes in 10%NBF. I will attach a schedule for you off
line.
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pru...@ihctech.net
Sent: Thursday, August 08, 2013 1:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] processing eyes
Can someone direct me to a protocol for processing eyes which have been
injected with Davidson's fixative and are now in 10% NBF. We had an eye
expert at the U for years I was going to send my techs to but apparently she
has finally retired because we cannot get a hold of her. Where are you Mary
Jo?
We need insight on how to process non-human primate eyes? They have been
injected with Davidson's fixative and are now in 10%NBF. We need a specific
processing schedule, embedding and sectioning advice.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
mailto:prueg...@hotmail.com prueg...@hotmail.com
mailto:rueggihcconsultin...@outlook.com rueggihcconsultin...@outlook.com
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Re: [Histonet] monkey control slides
It depends on the species. All monkeys are not created equal. Some primaries work on Rhesus but not Cyno. Sent from my iPhone On Oct 25, 2012, at 12:54 PM, Eric Hoy eric@utsouthwestern.edu wrote: Amy, Try MeDiCa in Carlsbad, CA. They have a variety of animal tissues for autoantibody detection. Their website, which is not very helpful, but will give you contact info: http://www.medica-dx.com Let me know if you can't get in touch with them. Eric Hoy === Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric@utsouthwestern.edu === On 10/25/12 5:29 PM, Amy Lee amylee...@yahoo.com wrote: Hello, I was asked to do IHC on monkey atrium, kidney and spleen or liver tissue. Could anybody tell me which company carry these monkey slides? Thanks! Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gross macro digital camera
Would appreciate suggestions for a good digital camera for taking macro photos during necropsy. Any takers? Thanks much. Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Neutrophil Antibodies
While on the topic of neuts-anyone have an antibody for canine neutrphils in ffpe? Sent from my iPhone On May 5, 2012, at 9:59 AM, John Shelley jshel...@sanfordburnham.org wrote: Hi All in Histoland, Problem solving never ends! I am working up two antibodies for neutrophils which are NIMP-R14 from Abcam which it is a rat monoclonal and also Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I have tried different conditions without any luck and was wondering if anyone has worked with these antibodies either from Abcam or another supplier and might have working conditions that have given results in the past. We are trying this on FFPE sections which the antibodies say they work on but I am thinking that I may try frozens. I was trying to avoid this however because I am using these antibodies on mouse WAT. Any help would be appreciated. Thanks!!! Happy Monday morning!!! Kind Regards! John J Shelley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unregistered HT testing
The pathologist is interpreting the staining result, right? The HT is only evaluating the stain quality, not the results. Sent from my iPhone On Apr 18, 2012, at 9:43 AM, Dessoye, Michael J mjdess...@commonwealthhealth.net wrote: Our interpretation of special stains/IHC/ISH is that it is the 'interpretation' of these stains that is 'high complexity'. I believe that CLIA is the place where 'high complexity' is defined (although CAP may expand on this) and CLIA does not recognize ASCP registration. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Konop, Nicole [mailto:nko...@chw.org] Sent: Tuesday, April 17, 2012 3:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shipping prepared histology slides
Can someone provide a quick reference to ANYTHING I can use to prove to my EHS that fixed, processed, coverslipped slides are not HAZMAT? They insist shipping finished slides cannot be performed by histotechs. Wha? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vet tissue processing
I'm looking for a histology processing lab who will process NBF veterinary tissues to H+E slides. I need a short turn around time, like 7-10 days. Any suggestions will be considered. Thanks, **Dell Deals: Treat yourself to a sweet deal on popular laptops! (http://pr.atwola.com/promoclk/100126575x1223100673x1201716527/aol?redir=http:%2F%2Faltfarm.mediaplex.com%2Fad%2Fck%2F12309%2D81939%2D1629%2D7) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
