Re: [Histonet] curling of kidney sections in water path
There is two things I do for kidney and liver tissue. 1. Add 5ml glycerol to the 100% Alcohol in the processor 2. After I face the block I soak the block in a dilution of liquid fabric softener. 40% fabric softener 60% water. If the first thing doesn't fix the problem then I move on to the second solution. */Science is organized knowledge. Wisdom is organized life./* */Immanuel Kant/* */(1724-1804)/* *Karen Bowden Staff Research Associate II*** *University of CA, San Diego//* *Department of Medicine* *9500 Gilman Dr. 0682* *La Jolla, CA 92093-0682* *858-534-0575 **/ CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./*** On 12/10/15 3:59 PM, mohamed abd el razik via Histonet wrote: dear all, i'm facing an issue that trouble us when we process tissue samples of different organs and cutting them; all organs are ok and spread fine and somoothly on ater path except for kidney sections (looks wrinkled and curled as if the praffin fram around it prevent it from spread!!!). i have tried using alcohol befor path and elevating the water temprature but no change in the sctions happened even after stay in the incubator) any suggestions please thank you for your help Mohamed Cairo University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] canine teeth
I would decal with TBD-2 for about two weeks. TBD-2 is a formic acid decal solution. I would shake the specimen 24 hrs a day. Vacuum and release the specimen several times a day. Change the decal every couple of days. Do not remove the paraffin it helps to protect from over decalcifying. *//* */-- You are what you do - not what you say --/* */Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 3525 John Hopkins Ct. 0863 San Diego, CA 92121-08630 858-822-1251 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./* On 4/26/11 10:48 AM, Perry, Margaret wrote: Hi I was given blocks today of a canine tooth that had been decaled for 8 days. We processed it on our regular overnight program. It was too hard to cut and popped out of the paraffin. The biopsy trim sheet did not indicate it was a tooth so I put the pieces in ammonium hydroxide for 10 min. It didn't help. Do you have any suggestion on how I can save this tissue? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] staining of plastic sections
I use McNeal as a substitute for HE. I use it on MMA embedded bone. It is to intense (for us) to be used on JB-4. I think you might need to try a few different stains to select the best. */ /* */-- You are what you do - not what you say --/* */Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 3525 John Hopkins Ct. 0863 San Diego, CA 92121-08630 858-822-1251 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./* Walters, Katherine S wrote: Perhaps you could try the Paragon stain. It looks more like an HE. Kathy Walters -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Wednesday, September 30, 2009 10:12 AM To: histonet@lists.utsouthwestern.edu Cc: Pete Finger Subject: [Histonet] staining of plastic sections Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried HE and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 100 micron sections
Hi, I am being asked to section paraffin embedded fat at 100 microns. I haven't sectioned anything in paraffin thicker than 20 microns. What are the tricks to get it to unroll in the water bath? -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER./* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fat histology
I work with bone and connective tissue. I have just been asked to process and section some fat. I would like to get some information on how to work with fat. Any information will be very helpful. -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER./* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opinions on motorized microtomes wanted
I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would like to get feedback from people who have used them. I have a problem with my shoulder and the continuous motion of using a rotory microtome is a issue for me. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opinions: motorized microtomes Thank you!!
Thank you for your responces. They have been very helpful. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. kbowden wrote: I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would like to get feedback from people who have used them. I have a problem with my shoulder and the continuous motion of using a rotory microtome is a issue for me. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bones
One of the departments (body donations) here uses bugs to clean soft tissue off of bones. I thinks it takes about a week or so. You might look into the type of bugs you have in your region for that purpose. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Ian Montgomery wrote: I have some bones, from various species, that I want to clean of muscle, tendons, etc, etc. The method currently used is boiling the bones in water for several hours, days until they are completely clean. Problem, it's a wee bit smelly, in fact a big bit smelly. Me being a delicate soul more used to various exotic eau de parfum wonder if there is another technique available. Some species respond to soaking for several weeks in laboratory detergent while others don't. NaOH or KOH, again some do others don't. What I would ideally like is a universal method that's reasonably quick, but not smelly, can anyone help. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] citrisolve?
I have used Citrisolve for serveral years. I don't think anything compares to Xylene, but I do think this comes very close. I change it about once a week. It pretty much deparaffinizes in the same amount of time. Disposal is dependent on the regulations in your area. When I started using it our Environmental Health and Safety said pour it down the drain, but as time goes by and with more regulations now I have to collect it and have it removed with hazardous waste. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Patten, Nicole (NIH/NIAAA) [F] wrote: Does anyone have any experience using Citrisolve as a Xylene alternative to deparaffinize tissue sections? Do I use it just like Xylene for the same amount of time? Can it be reused or should I use new Citrisolve each time? How do I dispose of it? Any help at all would be much appreciated. Thanks! Nicole J. Patten Post-Baccalaureate Fellow/IRTA National Institutes of Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
