I have used Citrisolve for serveral years. I don't think anything compares to Xylene, but I do think this comes very close. I change it about once a week. It pretty much deparaffinizes in the same amount of time. Disposal is dependent on the regulations in your area. When I started using it our Environmental Health and Safety said pour it down the drain, but as time goes by and with more regulations now I have to collect it and have it removed with hazardous waste.
--
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
858-534-4655 voice
858-534-5304 fax


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Patten, Nicole (NIH/NIAAA) [F] wrote:
Does anyone have any experience using Citrisolve as a Xylene alternative
to deparaffinize tissue sections? Do I use it just like Xylene for the
same amount of time? Can it be reused or should I use new Citrisolve
each time? How do I dispose of it?
Any help at all would be much appreciated. Thanks!

Nicole J. Patten
Post-Baccalaureate Fellow/IRTA
National Institutes of Health

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