Re: [Histonet] A Question About Paraffin Times

[Kari Simeone via Histonet]

I agree with Tony. I have on many occasions left the block baskets sit at room 
temperature (sat them in an OFF oven) and then turned the oven on to temp and 
re-embedded the tissue with no issues. You could also just return the basket to 
the warming tray until they are warm again to embed. 

Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor




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To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 162, Issue 17

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Today's Topics:

   1. Histotech and Histology Supervisor job openings in
  Birmingham, AL (Brannon Owens)
   2. Re: A Question About Paraffin Times (Tony Henwood (SCHN))
   3. Re: A Question About Paraffin Times (Garrey Faller)
   4. Decal Solution (Diana Martinez-Longoria)
   5. Histo job opportunity in a GI lab-Oak Lawn Illionios
  (dusko trajkovic)
   6. Grosser Needed in Southern California (Paula)


--

Message: 1
Date: Wed, 17 May 2017 19:40:39 +
From: Brannon Owens <bran...@alliedsearchpartners.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Histotech and Histology Supervisor job openings in
Birmingham, AL
Message-ID: <d5421f6f.d4340%bran...@alliedsearchpartners.com>
Content-Type: text/plain; charset="us-ascii"

We are currently seeking candidates for several openings in the Birmingham, AL 
area.  Relocation assistance is offered!

Position 1:  Histology Supervisor (3rd shift)
Position 2:  Histotechnician (1st shift)
Position 3:  Histotechnician (3rd shift)

Full job descriptions available on the Allied Search Partners website as well 
as additional opportunities in the field of histology.

We look forward to hearing from you!

Thank you,


To view a complete list of Allied Search Partners current openings go to:  
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.alliedsearchpartners.com_careers_=DQICAg=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4=OYAKUwWjo2B60ZnbVZNPcYLy-11xcbih2bPn7znIoII=

Brannon Owens
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Allied Search Partners
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--

Message: 2
Date: Wed, 17 May 2017 23:26:24 +
From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au>
To: P Sicurello <pat...@gmail.com>
Cc: "histonet@lists.utsouthwestern.edu"
    <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] A Question About Paraffin Times
Message-ID:
<0237449de79dbc45b686ab82cdcd16ff95554...@svdcmbx-mex008.nswhealth.net>

Content-Type: text/plain; charset="us-ascii"

It is best to remove the blocks and let them set at room temperature.

Leaving them at 60 or more degrees can adversely affect antigens.

We routinely do this with our autopsies and have not noted any issues.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fa

Re: [Histonet] A Question About Paraffin Times

[Garrey Faller via Histonet]

I have had to deal with this in the past and could not find an answer .
My intuition was that it can't be good to leave in hot wax. So, on the rare 
occasion when my only Histotech called in sick I had to take the blocks out and 
cool them down. When ready they were put back in the tissue embedder to heat up 
and everything was fine. That's my experience,
Garrey

Sent from my iPhone

> On May 16, 2017, at 2:48 PM, Cindy Bird via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> I agree?
> 
> Sent from my iPhone
> 
>> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet 
>> <histonet@lists.utsouthwestern.edu> wrote:
>> 
>> Hello Paula,
>> So why must the cassettes be removed on Saturday morning if you aren't 
>> embedding until Monday morning?
>> Don't you have a "delay start" feature on your processor? Neither option A 
>> or B seem like a good thing for the tissue!
>> We time our processor to finish at 5 AM Monday when the first Histotech 
>> arrives for embedding.
>> The cassettes remain in formalin until the processor starts up on Sunday 
>> evening.
>> 
>> Regards,
>> 
>> 
>> Shannon H. Logan  B.S., HTL (ASCP)
>> Pathology Department
>> 
>> Bellin Health Memorial Hospital
>> 744 South Webster Avenue
>> Green Bay, WI 54305-3400
>> 920-433-3653  X3727
>> 
>> 
>> 
>> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
>> Sent: Tuesday, May 16, 2017 10:34 AM
>> To: HistoNet
>> Subject: [Histonet] A Question About Paraffin Times
>> 
>> Good Morning Listers,
>> 
>> I am asking the collective wisdom of the Histonet this question:
>> 
>> 
>> Is it better to remove baskets from the processor on Saturday morning and:
>> 
>> A. Let the cassettes freeze, then melt them down and embed Monday morning?
>> OR
>> B. Leave the cassettes in molten paraffin and embed Monday morning?
>> 
>> 
>> I am of the opinion that leaving the samples (not fatty, like breast cores)
>> in molten paraffin (62 degrees C) is bad practice, and causes them to get
>> "crunchy", among other things.
>> 
>> What do you think?
>> 
>> Thank in advance.
>> 
>> Sincerely,
>> 
>> Paula Sicurello, HTL (ASCP)CM
>> 
>> Histotechnology Specialist
>> 
>> UC San Diego Health
>> 
>> 200 Arbor Drive
>> 
>> San Diego, CA 92103
>> 
>> (P): 619-543-2872 <#>
>> 
>> 
>> 
>> *Confidentiality Notice*: The information transmitted in this e-mail is
>> intended only for the person or entity to which it is addressed and may
>> contain confidential and/or privileged material. Any review,
>> retransmission, dissemination or other use of or taking of any action in
>> reliance upon this information by persons or entities other than the
>> intended recipient is prohibited. If you received this e-mail in error,
>> please contact the sender and delete the material from any computer.
>> ___
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Re: [Histonet] A Question About Paraffin Times

[Tony Henwood (SCHN) via Histonet]

It is best to remove the blocks and let them set at room temperature.

Leaving them at 60 or more degrees can adversely affect antigens.

We routinely do this with our autopsies and have not noted any issues.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 17 May 2017 1:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores) in 
molten paraffin (62 degrees C)  is bad practice, and causes them to get 
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is 
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Re: [Histonet] A Question About Paraffin Times

[Weems, Joyce K. via Histonet]

And that should say MUCH better..

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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regarding the error in a separate email. 


-Original Message-
From: Weems, Joyce K. via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 16, 2017 3:15 PM
To: P Sicurello <pat...@gmail.com>
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] A Question About Paraffin Times

Must better to remove them on Sat than to leave in the hot paraffin. We do this 
all the time so we can meet the CAP time in formalin for the breasts.

Good luck! j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
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intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:34 AM
To: HistoNet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores) in 
molten paraffin (62 degrees C)  is bad practice, and causes them to get 
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is 
intended only for the person or entity to which it is addressed and may contain 
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dissemination or other use of or taking of any action in reliance upon this 
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Re: [Histonet] A Question About Paraffin Times

[Cindy Bird via Histonet]

I agree?

Sent from my iPhone

> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> Hello Paula,
> So why must the cassettes be removed on Saturday morning if you aren't 
> embedding until Monday morning?
> Don't you have a "delay start" feature on your processor? Neither option A or 
> B seem like a good thing for the tissue!
> We time our processor to finish at 5 AM Monday when the first Histotech 
> arrives for embedding.
> The cassettes remain in formalin until the processor starts up on Sunday 
> evening.
> 
> Regards,
> 
> 
> Shannon H. Logan  B.S., HTL (ASCP)
> Pathology Department
> 
> Bellin Health Memorial Hospital
> 744 South Webster Avenue
> Green Bay, WI 54305-3400
> 920-433-3653  X3727
> 
> 
> 
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Tuesday, May 16, 2017 10:34 AM
> To: HistoNet
> Subject: [Histonet] A Question About Paraffin Times
> 
> Good Morning Listers,
> 
> I am asking the collective wisdom of the Histonet this question:
> 
> 
> Is it better to remove baskets from the processor on Saturday morning and:
> 
> A. Let the cassettes freeze, then melt them down and embed Monday morning?
> OR
> B. Leave the cassettes in molten paraffin and embed Monday morning?
> 
> 
> I am of the opinion that leaving the samples (not fatty, like breast cores)
> in molten paraffin (62 degrees C) is bad practice, and causes them to get
> "crunchy", among other things.
> 
> What do you think?
> 
> Thank in advance.
> 
> Sincerely,
> 
> Paula Sicurello, HTL (ASCP)CM
> 
> Histotechnology Specialist
> 
> UC San Diego Health
> 
> 200 Arbor Drive
> 
> San Diego, CA 92103
> 
> (P): 619-543-2872 <#>
> 
> 
> 
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited. If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> ___
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Re: [Histonet] A Question About Paraffin Times

[Seeley, Heather via Histonet]

We do a delay protocol as well. The tissue samples will sit in formalin and 
start processing Sunday night for a normal amount of time to come off Monday 
morning. If this is not a possibility, it would be better to let them harden in 
the paraffin than to leave them in the hot paraffin, as this will lead to the 
tissue becoming brittle and very difficult to section. 

HEATHER SEELEY, HT(ASCP)
Histotech
803-985-4676 OFFICE
803-327-7598 FAX



From: P Sicurello [pat...@gmail.com]
Sent: Tuesday, May 16, 2017 11:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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Re: [Histonet] A Question About Paraffin Times

[Weems, Joyce K. via Histonet]

Must better to remove them on Sat than to leave in the hot paraffin. We do this 
all the time so we can meet the CAP time in formalin for the breasts.

Good luck! j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:34 AM
To: HistoNet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores) in 
molten paraffin (62 degrees C)  is bad practice, and causes them to get 
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is 
intended only for the person or entity to which it is addressed and may contain 
confidential and/or privileged material.  Any review, retransmission, 
dissemination or other use of or taking of any action in reliance upon this 
information by persons or entities other than the intended recipient is 
prohibited.  If you received this e-mail in error, please contact the sender 
and delete the material from any computer.
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Re: [Histonet] A Question About Paraffin Times

[Garcia, Lori, M.Sc. via Histonet]

Hi Paula,

I am in agreement with everyone else about putting the processor on a delay so 
it will finish when needed. However, if that is not possible, it is perfectly 
fine for the blocks to harden at room temp, not freezing, and then be warmed up 
again for embedding. This has come up in my research lab, and we haven't had 
any problems.

Regards,
Lori

This message has been marked as Medtronic Controlled
-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:30 AM
To: Logan, Shannon <shannon.lo...@bellin.org>
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] A Question About Paraffin Times

Hi Shannon and Everyone Else,

Usually the samples are placed on a weekend delay.  This time, however, the 
breast cases were sitting in formalin since Thursday and the run had to end on 
Saturday due to the CAP 72 hour rule for breast and HER2 testing.

The problem is, the person in charge of Histology not a Histologist.  The 
person in charge was told by several experienced histotechs that letting the 
samples sit in molten paraffin is not a good thing to do.  They were told that 
it was routine (which it isn't) to let them sit in hot wax for days.

I just need information that states that sitting in paraffin for any excessive 
length of time is bad.  I found it mentioned in Sheehan and Hrapcek's "Theory 
and Practice of Histotechnology".

I can't find my Carson.  Does she mention excessive times in paraffin?

If so, please let me know.

Thank you for all the assistance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is 
intended only for the person or entity to which it is addressed and may contain 
confidential and/or privileged material.  Any review, retransmission, 
dissemination or other use of or taking of any action in reliance upon this 
information by persons or entities other than the intended recipient is 
prohibited.  If you received this e-mail in error, please contact the sender 
and delete the material from any computer.

On Tue, May 16, 2017 at 11:13 AM, Logan, Shannon <shannon.lo...@bellin.org>
wrote:

> Hello Paula,
>
> So why must the cassettes be removed on Saturday morning if you aren’t
> embedding until Monday morning?
>
> Don’t you have a “delay start” feature on your processor? Neither
> option A or B seem like a good thing for the tissue!
>
>  We time our processor to finish at 5 AM Monday when the first
> Histotech arrives for embedding.
>
> The cassettes remain in formalin until the processor starts up on
> Sunday evening.
>
>
>
> Regards,
>
>
>
>
>
> Shannon H. Logan  B.S., HTL (ASCP)
>
> Pathology Department
>
>
>
> Bellin Health Memorial Hospital
>
> 744 South Webster Avenue
>
> Green Bay, WI 54305-3400
>
> 920-433-3653  X3727 <(920)%20433-3653>
>
>
>
>
>
>
>
> *From:* P Sicurello via Histonet
> [mailto:histonet@lists.utsouthwestern.edu]
>
> *Sent:* Tuesday, May 16, 2017 10:34 AM
> *To:* HistoNet
> *Subject:* [Histonet] A Question About Paraffin Times
>
>
>
> Good Morning Listers,
>
> I am asking the collective wisdom of the Histonet this question:
>
>
> Is it better to remove baskets from the processor on Saturday morning and:
>
> A. Let the cassettes freeze, then melt them down and embed Monday morning?
> OR
> B. Leave the cassettes in molten paraffin and embed Monday morning?
>
>
> I am of the opinion that leaving the samples (not fatty, like breast
> cores) in molten paraffin (62 degrees C) is bad practice, and causes
> them to get "crunchy", among other things.
>
> What do you think?
>
> Thank in advance.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872 <(619)%20543-2872> <#>
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail
> is intended only for the person or entity to which it is addressed and
> may contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action
> in reliance upon this information by persons or entities other than
> the intended recipient is prohibited. If you received this e-mail in
> error, please contact the sender and delete the material from any computer.
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

Re: [Histonet] A Question About Paraffin Times

[P Sicurello via Histonet]

Hi Shannon and Everyone Else,

Usually the samples are placed on a weekend delay.  This time, however,
the breast cases were sitting in formalin since Thursday and the run had to
end on Saturday due to the CAP 72 hour rule for breast and HER2 testing.

The problem is, the person in charge of Histology not a Histologist.  The
person in charge was told by several experienced histotechs that letting
the samples sit in molten paraffin is not a good thing to do.  They were
told that it was routine (which it isn't) to let them sit in hot wax for
days.

I just need information that states that sitting in paraffin for any
excessive length of time is bad.  I found it mentioned in Sheehan and
Hrapcek's "Theory and Practice of Histotechnology".

I can't find my Carson.  Does she mention excessive times in paraffin?

If so, please let me know.

Thank you for all the assistance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
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intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.

On Tue, May 16, 2017 at 11:13 AM, Logan, Shannon <shannon.lo...@bellin.org>
wrote:

> Hello Paula,
>
> So why must the cassettes be removed on Saturday morning if you aren’t
> embedding until Monday morning?
>
> Don’t you have a “delay start” feature on your processor? Neither option A
> or B seem like a good thing for the tissue!
>
>  We time our processor to finish at 5 AM Monday when the first Histotech
> arrives for embedding.
>
> The cassettes remain in formalin until the processor starts up on Sunday
> evening.
>
>
>
> Regards,
>
>
>
>
>
> Shannon H. Logan  B.S., HTL (ASCP)
>
> Pathology Department
>
>
>
> Bellin Health Memorial Hospital
>
> 744 South Webster Avenue
>
> Green Bay, WI 54305-3400
>
> 920-433-3653  X3727 <(920)%20433-3653>
>
>
>
>
>
>
>
> *From:* P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
>
> *Sent:* Tuesday, May 16, 2017 10:34 AM
> *To:* HistoNet
> *Subject:* [Histonet] A Question About Paraffin Times
>
>
>
> Good Morning Listers,
>
> I am asking the collective wisdom of the Histonet this question:
>
>
> Is it better to remove baskets from the processor on Saturday morning and:
>
> A. Let the cassettes freeze, then melt them down and embed Monday morning?
> OR
> B. Leave the cassettes in molten paraffin and embed Monday morning?
>
>
> I am of the opinion that leaving the samples (not fatty, like breast cores)
> in molten paraffin (62 degrees C) is bad practice, and causes them to get
> "crunchy", among other things.
>
> What do you think?
>
> Thank in advance.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872 <(619)%20543-2872> <#>
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited. If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
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Re: [Histonet] A Question About Paraffin Times

[Logan, Shannon via Histonet]

Hello Paula,
So why must the cassettes be removed on Saturday morning if you aren't 
embedding until Monday morning?
Don't you have a "delay start" feature on your processor? Neither option A or B 
seem like a good thing for the tissue!
 We time our processor to finish at 5 AM Monday when the first Histotech 
arrives for embedding.
The cassettes remain in formalin until the processor starts up on Sunday 
evening.

Regards,


Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727



From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 10:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A. Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B. Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C) is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material. Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited. If you received this e-mail in error,
please contact the sender and delete the material from any computer.
___
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Re: [Histonet] A Question About Paraffin Times

[cynthia haynes via Histonet]

Please don't leave the tissue in the hot wax.They will harden and be brittle. 
Cynthia James H.T 

Sent from Yahoo Mail on Android 
 
  On Tue, May 16, 2017 at 10:50 AM, P Sicurello via 
Histonet wrote:   Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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Re: [Histonet] A Question About Paraffin Times

[Rene J Buesa via Histonet]

I consider taking out → freezing →  melting → casting blocks is worse than 
leaving the tissues in molten paraffin.It seems that your Monday tissue 
processing ends on Saturday. I suggest to process your tissues with a "delay" 
(most tissue processers have this feature) and leave  them more time in 
formalin and make coincide their ending time with your Monday start time.René 

On Tuesday, May 16, 2017 11:51 AM, P Sicurello via Histonet 
 wrote:
 

 Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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[Histonet] A Question About Paraffin Times

[P Sicurello via Histonet]

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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