Re: [Histonet] Bleaching in the histo lab

2012-02-01 Thread Kim Donadio
Maybe those scented bleach wiped would work. I love those things and they smell 
pretty good.  Just a thought. I like the greencan fresh scent
products/clorox-disinfecting-wipes/
KimD
Sent from my iPhone

On Feb 1, 2012, at 6:03 PM, "Lee & Peggy Wenk"  wrote:

> 
> - Need to clean off the corrosive bleach off the surfaces. That would also

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Re: [Histonet] Bleaching in the histo lab

2012-02-01 Thread Lee & Peggy Wenk

Several questions and comments, in no particular order:

1. What percent of bleach?
- 10% is all that is needed for biohazards. If you are concerned about the 
smell, it might be too high a percent.


2. How good is your ventilation? How long do you continue to smell the 
chlorine?
- If you continue to smell it hours later, or even the next day, have your 
safety officer and maintenance people check out the ventilation.


3. After wiping down with 10% bleach, are you wiping down the counter with 
water?
- Need to clean off the corrosive bleach off the surfaces. That would also 
help with the smell. But takes more time.


3. What locations in the lab are you cleaning with dilute bleach?
- The only areas that need to be cleaned with a disinfectant are those areas 
that have fresh or not completely fixed tissue, so around the grossing 
stations and the cryostat. Maybe where specimens are received into the lab.
- The areas where you process tissue, embed, microtome, do staining, file 
slides and blocks should not need to be disinfected with bleach. The tissue 
has been fixed in formalin, and gone through alcohol, xylene (or 
substitute), and placed in 60 degree C (140 degree F) paraffin. That should 
kill almost all microorganisms. Therefore, should not need to clean up with 
anything beyond soap and water. If you have a very underprocessed tissue 
block, and it's oozing and weeping all over the counter and microtome, you 
may want to disinfect the area. (If it's a CJD case, you are going to need 
strong solutions than 10% bleach, but that's a whole new conversation.)
- So talk with your safety officer, about how there are no biohazards in the 
other parts of the lab. They may be thinking more of the clinical pathology 
labs, with blood tubes and petri dishes, needing to be disinfected with 
bleach every day/shift.


4. Chemical incompatibility:
Bleach is incompatible with ammonia (makes chlorine gas - deadly)
Bleach is incompatible with acids
Bleach is an oxidizer, and formaldehyde is supposed to be kept away from 
oxidizers.
So, yes, I would be a little worried about chemical interaction. However, 
wiping down the area first with water, to remove other chemicals, before the 
bleach, would take care of this problems.


5. What does Epidemiology suggest for disinfectant?
Our epidemiology is suggesting other cleaning solutions for disinfecting, 
rather than bleach, in many cases.

- not as corrosive
- less obnoxious fumes
- more "green"
- better disinfectant and faster, than bleach

Peggy Wenk, HTL(ASCP)SLS
Beaumont Health Systems
Royal Oak, MI 48073
(Comments reflect my opinions, not that of my hospital)

-Original Message- 
From: angela smith

Sent: Wednesday, February 01, 2012 8:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bleaching in the histo lab

I have been told by our safety officer that it is standard practice too 
clean the lab at the end of the day with diluted bleach. I have noticed a 
chemical reaction (smell) when cleaning the main area of the lab. I have 
concerns that this is not a good practice due to chemical reactions as we 
use so many chemicals in histology. What do other people do?  Also I believe 
it is unsafe to use bleach with anything formalin related.
Please let me know if you have a "standard" practice or mandated cleaning 
from your facility.

Angela
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RE: [Histonet] Bleaching in the histo lab

2012-02-01 Thread WILLIAM DESALVO

The short answer is that you need a detailed procedure for all immediate and 
regular cleaning of infectious materials and hazardous chemicals used in your 
lab.
 
I believe the standard practice your safety officer is referring used in the 
clinical lab is a practice to clean all surfaces after each shift to 
remove/decontaminate all contaminated or potentially contaminated from the work 
surfaces of blood or other infectious material w/ 10% bleach solution (1:10 
dilution of 5.25% solution of sodium hypochlorite) or other lab cleaners 
approved for biohazard approved contamination. The waste generated by this 
process should be disposed of in the non-regulated medical waste. Typically in 
the Histology room there should not be blood or other infectious materials (you 
are working w/ fixed and processed tissue samples), unless you have your frozen 
section and/or gross dissection processes connected to and part of the main 
Histology room. I suggest you use the bleach solution whenever there is known 
or suspicion of contamination of a potentially infectious material.
 
For areas/surfaces and equipment where lab chemicals are used, always remove 
the spilled chemical according to MSDS recommendations and then clean the area 
w/ a damp cloth with water and a detergent and them wipe clean and dry. If or 
when a hazardous chemical is spilled (i.e. Xylene, Formalin or chemicals 
associated w/ IHC or Special staining), it should be treated as a hazardous 
chemical spill and there area should be cleaned according to your hazardous 
chemical spill protocol. Small spills (up to 300 cc) - neutralize and/or 
adsorption; medium spill (300 cc to 5 liters) - adsorption spill kit; Large 
spill (>5 liters) - outside help. disposal will be in regulated hazardous waste.
 
The main point here, instances of contamination of infectious materials or any 
size spill of hazardous chemicals should be treated seriously and properly. 
Your cleaning and disposal procedure must be very detailed to protect the 
employees and meet lab, municipality, state and regulatory requirements. I am 
very passionate about properly handling chemicals and protecting everyone that 
must have contact w/ these necessary solutions/products. I suggest you and your 
safety officer have a sit down and discuss how to document and address these 
issues, a drive-by by a safety officer is really not adequate.
  
William DeSalvo, B.S., HTL(ASCP)

 

> Date: Wed, 1 Feb 2012 05:57:27 -0800
> From: we3smi...@yahoo.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Bleaching in the histo lab
> 
> I have been told by our safety officer that it is standard practice too clean 
> the lab at the end of the day with diluted bleach. I have noticed a chemical 
> reaction (smell) when cleaning the main area of the lab. I have concerns that 
> this is not a good practice due to chemical reactions as we use so many 
> chemicals in histology. What do other people do?  Also I believe it is unsafe 
> to use bleach with anything formalin related. 
> Please let me know if you have a "standard" practice or mandated cleaning 
> from your facility.
> Angela 
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> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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[Histonet] Bleaching in the histo lab

2012-02-01 Thread angela smith
I have been told by our safety officer that it is standard practice too clean 
the lab at the end of the day with diluted bleach. I have noticed a chemical 
reaction (smell) when cleaning the main area of the lab. I have concerns that 
this is not a good practice due to chemical reactions as we use so many 
chemicals in histology. What do other people do?  Also I believe it is unsafe 
to use bleach with anything formalin related. 
Please let me know if you have a "standard" practice or mandated cleaning from 
your facility.
Angela
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