Re: [Histonet] Coverslip Instruments
We use the Tissue Tek Glas with our Ventana labeled slides with no problem. The key is to insure the label does not hang off the edge in the slightest. However, it does not put them in a tray, however, that function only takes about 10 seconds. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion Message: 2 Date: Fri, 17 Mar 2023 21:11:20 + From: "Dooley, Elaine O." Subject: [Histonet] coverslip machines Hi everyone, Does anyone know of a cover slipping machine other than the Roche HE 600, in which the slides are loaded in 20 folder type trays. The slides are not picked up by the machine, rather the coverslip is applied to the slides in the folder. Elaine Dooley Shands Labs at Rocky Point 352-265-0111 ext 72117 Or does anyone have good luck putting slides from a Ventana ultra with clear plastic overlays on an automated coverslipper? Our old coverslippers always had problems with the labels sticking to the robotic arm that lifts the slide. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslip machines
Hi everyone, Does anyone know of a cover slipping machine other than the Roche HE 600, in which the slides are loaded in 20 folder type trays. The slides are not picked up by the machine, rather the coverslip is applied to the slides in the folder. Elaine Dooley Shands Labs at Rocky Point 352-265-0111 ext 72117 Or does anyone have good luck putting slides from a Ventana ultra with clear plastic overlays on an automated coverslipper? Our old coverslippers always had problems with the labels sticking to the robotic arm that lifts the slide. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip tape
Hello: Is there a way to get a small free sample of that tape? Does it save money or have some other pluses? Gratefully, Jorge On Fri, Oct 9, 2015 at 9:34 AM, Abbott, Tanya via Histonet < histonet@lists.utsouthwestern.edu> wrote: > This is more a Cyto question, but, does anyone out there use the Sakura > Coverslip tape to coverslip their Thin Preps? We seem to be having a > problem down the sides of the slide where the tape is not being pushed down > and access glue seems to accumulate there. Sakura tells us it's a drying > issue but I really don't think it is, we let the slides dry long enough. It > seems to me that the bar that pushes the tape down is not pushing down well > enough on the sides. We have changed the springs recently and are on a new > roll of tape. Our H slides don't seem to be affected. > Any input is appreciated! Happy Friday! > Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > Penn State Health St. Joseph > (phone) 610-378-2635 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip tape
This is more a Cyto question, but, does anyone out there use the Sakura Coverslip tape to coverslip their Thin Preps? We seem to be having a problem down the sides of the slide where the tape is not being pushed down and access glue seems to accumulate there. Sakura tells us it's a drying issue but I really don't think it is, we let the slides dry long enough. It seems to me that the bar that pushes the tape down is not pushing down well enough on the sides. We have changed the springs recently and are on a new roll of tape. Our H slides don't seem to be affected. Any input is appreciated! Happy Friday! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip
Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter. It has to do with the coverslip using the resin tape. However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys. It looks almost like when water freezes and gives that frosted look. Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip
We have found this to be a culmination of a couple of things: Bad lot of tape from vendor Drying slides before cover slipping Too much xylene drip during coverslip (dilutes the glue stuff on the tape allowing for weak adhesive) We use Sakura cover slipping tape for more consistent quality and adjust xylene drip accordingly and make sure slides do not sit out very long at all as xylene from the stainer will evaporate (drying slides) rather quickly. Hope this helps. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/25/14, 7:47 AM, Tommy Mai t...@phenopath.com wrote: Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter. It has to do with the coverslip using the resin tape. However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys. It looks almost like when water freezes and gives that frosted look. Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip
Usually these odd results have 2 origins that you should look into: 1- using a different (cheaper and harder) plastic than the originally recommended; and 2- a regulation problem in the amount of xylene dropped into the film René J. On Friday, July 25, 2014 9:47 AM, Tommy Mai t...@phenopath.com wrote: Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter. It has to do with the coverslip using the resin tape. However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys. It looks almost like when water freezes and gives that frosted look. Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip Sealant
Hi there, Does anybody know of a good alternative to nail polish for sealing round coverslips (or any shape, for that matter!). Ideally I am looking for a solution that is both an aqueous mount media and sealant, if such a beast exists... Thanks! Gil PS: Happy New Year! -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Coverslip Sealant
ProLong Gold (Invitrogen) Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Guillermo Palchik Sent: Friday, December 30, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslip Sealant Hi there, Does anybody know of a good alternative to nail polish for sealing round coverslips (or any shape, for that matter!). Ideally I am looking for a solution that is both an aqueous mount media and sealant, if such a beast exists... Thanks! Gil PS: Happy New Year! -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslip for in-situ hybridization
Hello, Histoneter, Do you mind to tell me where are you buying coverslip for in-situ hybridization probe incubation? What's the catalogue number? Thank you very much for sharing your information. Best, Xu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip removal question
Hi Histonetters When I soak my slides in xylene to remove the coverslip, do I then need to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and then H20. And then drying and re applying permount? Or can I go straight from xylene to drying to re-coversliping with permount? Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip removal question
No, once you have removed the coverslip, just wash the section thoroughly in xylene to remove any thick remnant of the old permount, and reapply permount and cover. René J. --- On Fri, 10/22/10, Lewis, Patrick patrick.le...@seattlechildrens.org wrote: From: Lewis, Patrick patrick.le...@seattlechildrens.org Subject: [Histonet] Coverslip removal question To: Histonet@lists.utsouthwestern.edu Date: Friday, October 22, 2010, 3:55 PM Hi Histonetters When I soak my slides in xylene to remove the coverslip, do I then need to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and then H20. And then drying and re applying permount? Or can I go straight from xylene to drying to re-coversliping with permount? Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip Film
Hello Does anyone use a non-Sakura coverslip film on your Sakura coverslipper? My boss handed me a flyer from a company that sells film and it's a lot less money than the Sakura brand. Thanks, Paula Lab Manager Path Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip Film
Once I tried one and it was harder, reacted less readily with xylene, and tended to peel-off easier. You could ask for a sample and try it. René J. --- On Thu, 10/7/10, Paula Lucas plu...@biopath.org wrote: From: Paula Lucas plu...@biopath.org Subject: [Histonet] Coverslip Film To: histonet@lists.utsouthwestern.edu Date: Thursday, October 7, 2010, 12:45 PM Hello Does anyone use a non-Sakura coverslip film on your Sakura coverslipper? My boss handed me a flyer from a company that sells film and it's a lot less money than the Sakura brand. Thanks, Paula Lab Manager Path Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslip removal
I am using aqueous mounting media, faramount, for the first time. The slides I coverslipped several days ago look dry. Will soaking in water remove the coverslip? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslip issues
Hi all, I'm looking for some help in choosing a new vendor for coverslips for the Thermo Consul coverslipper. We have been buying coverslips from Thermo since we bought the instrument, and the last shipment (of 10 cases) is from a new supplier and is not working in the instrument. The coverslip cannot be detected and the mountant is not dispensing. I spoke with the technician (Diane is wonderful) and she walked me through a diagnostic on the Consul and the instrument is not the problem. Does anyone out there have a suggestion as to a coverslip that they know works in the Consul? I have already tried the Vista from VWR (which is okay, but not perfect) and am eagerly awaiting requested samples. Thanks for your help. Denise Crowley Facility Manager Histology David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-427 Cambridge MA 02139 617-258-8183 [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
