Re: [Histonet] Coverslipping mystery
We have a CV5030 and use the suggested Surgipath micromount. It works well. As has been previously mentioned, you can adjust the amount of mountant dispensed as desired. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: Saturday, July 11, 2015 9:18 AM To: John Kiernan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
It sounds totally plausible, but just to put a spanner in the works; I have a plastic pipette in my DPX that I leave in there for at least 6 months and I don't get that artifact. We may have different pipettess though. Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 13, 2015, at 12:30 AM, Adam Boanas a.boa...@epistem.co.uk wrote: Hello again, Thought I might offer an update on the coverslipping issue as it might be of use in future. I ran a test last week of manual coverslipping using blank charged and un-charged slides and using DPX and Pertex as the mountant. I also used 2 methods of application. 1) Mountant applied using plastic Pasteur pipette 2) Mountant applied using aluminium screw cap tube. Following immersion in xylene for 5 mins the coverslips were applied. From viewing this morning, all slides were clear with the exception of those coverslipped using DPX applied with the Pasteur. In each case, these slides had the 'parched earth' artefact having been left to dry over the weekend. I suspect that the DPX has had a reaction with the plastic of the pipette during application and the artefact is caused by residual `molten` plastic from the pipette that only reveals itself over time. Does this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) Thanks Adam -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: 11 July 2015 15:18 To: John Kiernan Cc: Adam Boanas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman
Re: [Histonet] Coverslipping mystery
Hello again, Thought I might offer an update on the coverslipping issue as it might be of use in future. I ran a test last week of manual coverslipping using blank charged and un-charged slides and using DPX and Pertex as the mountant. I also used 2 methods of application. 1) Mountant applied using plastic Pasteur pipette 2) Mountant applied using aluminium screw cap tube. Following immersion in xylene for 5 mins the coverslips were applied. From viewing this morning, all slides were clear with the exception of those coverslipped using DPX applied with the Pasteur. In each case, these slides had the 'parched earth' artefact having been left to dry over the weekend. I suspect that the DPX has had a reaction with the plastic of the pipette during application and the artefact is caused by residual `molten` plastic from the pipette that only reveals itself over time. Does this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) Thanks Adam -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: 11 July 2015 15:18 To: John Kiernan Cc: Adam Boanas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
Adam: Just guessing, but I think the mystery is caused by how fluid the DPX is. Guessing again, but it probably is more dense as it should.I would dilute it to the lowest density it can be used in your coverslipper. Give it a try.René On Thursday, July 9, 2015 9:40 AM, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
I am not familiar with DPX, but is there a compatibility issue between it and the slide clearant you are using? Tresa -Original Message- From: Adam Boanas [mailto:a.boa...@epistem.co.uk] Sent: Thursday, July 09, 2015 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipping mystery Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this o ccurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
Look in your manual, near the back. There should be mountant type calibration settings for yo to adjust the machine to. That, hopefully will solve your issue! Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -Original Message- From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, July 09, 2015 9:47 AM To: Adam Boanas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery Adam: Just guessing, but I think the mystery is caused by how fluid the DPX is. Guessing again, but it probably is more dense as it should.I would dilute it to the lowest density it can be used in your coverslipper. Give it a try.René On Thursday, July 9, 2015 9:40 AM, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
What type of clearing agent are you using? The aliphatic hydrocarbons are not compatible with all mounting media. From: Adam Boanas a.boa...@epistem.co.uk To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 07/09/2015 06:16 AM Subject:[Histonet] Coverslipping mystery Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance whe n this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslipping mystery
Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this o ccurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
