Re: [Histonet] Cut FFPE Slides for ISH

[Michael Gudo via Histonet]

Hello Jenniffer,

we store the slides with section at 4-8 °C for many month or even longer and we 
do not have any problems with ISH.

Best regards
Michael



> Am 28.08.2017 um 17:30 schrieb Jennifer Phinney via Histonet 
> :
> 
> Hello Histonet,
> I am hoping someone can point me in the right direction to find some 
> references for how long FFPE slides intended for ISH can be kept before 
> staining.  I have a researcher who swears their slides have to be stained 
> within 24 hours of being cut, which does not sound correct to me.  I've used 
> ACD's RNAscope before and their protocol lists the timeframe as 3 months (so 
> this is a bit of a difference).
> 
> There's a lot of information about fixation times, but I've come up empty 
> handed for cut slides.
> 
> Thanks everyone!
> 
> Jennifer Phinney QIHCCM
> Project Coordinator
> Kansas State University
> Veterinary Diagnostic Lab
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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[Histonet] Cut FFPE Slides for ISH

[Dorothy Hu via Histonet]

Hi Jennifer,

My ISH slides (FFPE) were stored in -20oC for several months or more than
one year and the targets were less abundant. I didn't use RNAscope though,
which is more sensitive. I don't know what kind of RNA your researcher try
to locate and need to do ISH within 24 hours after cutting?  Fresh cut
section will yield more signal for sure. I think as long as you have a few
copies of genes in your tissue, RNAscope should be able to detect.

Dorothy Hu
HSDM







> Message: 2
> Date: Mon, 28 Aug 2017 15:30:08 +
> From: "Jennifer  Phinney" <jh...@vet.k-state.edu>
> To: "Histonet@lists.utsouthwestern.edu"
> <Histonet@lists.utsouthwestern.edu>
> Subject: [Histonet] Cut FFPE Slides for ISH
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> namprd05.prod.outlook.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonet,
> I am hoping someone can point me in the right direction to find some
> references for how long FFPE slides intended for ISH can be kept before
> staining.  I have a researcher who swears their slides have to be stained
> within 24 hours of being cut, which does not sound correct to me.  I've
> used ACD's RNAscope before and their protocol lists the timeframe as 3
> months (so this is a bit of a difference).
>
> There's a lot of information about fixation times, but I've come up empty
> handed for cut slides.
>
> Thanks everyone!
>
> Jennifer Phinney QIHCCM
> Project Coordinator
> Kansas State University
> Veterinary Diagnostic Lab
>
>
>
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[Histonet] Cut FFPE Slides for ISH

[Jennifer Phinney via Histonet]

Hello Histonet,
I am hoping someone can point me in the right direction to find some references 
for how long FFPE slides intended for ISH can be kept before staining.  I have 
a researcher who swears their slides have to be stained within 24 hours of 
being cut, which does not sound correct to me.  I've used ACD's RNAscope before 
and their protocol lists the timeframe as 3 months (so this is a bit of a 
difference).

There's a lot of information about fixation times, but I've come up empty 
handed for cut slides.

Thanks everyone!

Jennifer Phinney QIHCCM
Project Coordinator
Kansas State University
Veterinary Diagnostic Lab
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