Re: [Histonet] Derm IHC question

2019-02-10 Thread Tony Henwood (SCHN) via Histonet 
Possibly, the edges have been allowed to dry prior to immersion in fixative.

Also is there evidence of cautery artefact?


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: Debra Siena via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Saturday, 9 February 2019 3:51 AM
To: 'histonet'
Subject: [Histonet] Derm IHC question

Hello fellow Histonetters
I would like to ask you a question about IHC staining and derm cases.  I am 
seeing a peculiar issue going on, where the melanocytes in the middle of the 
tissues are staining pretty well but when you get to the ends of the tissues 
either shaves or ellipses, they are not staining. This is sporadic, not every 
case and there is no consensus as to a common thread between the cases.I 
feel that this may be a fixation issue but was just wondering if anyone had 
ever seen the same phenomena and would be willing to share the theory or even 
better what was the remedy behind this issue.  The fixative is 10% Neutral 
Buffered Formaliln  and the cells in question that are "dropping out" which is 
what the pathologist is describing are melanocytes, especially with Sox-10 and 
Mart 1 antibodies.
Thanks for the assistance, I definitely appreciate it very much.

Best wishes,

[image001]  Debbie Siena, HT(ASCP)QIHC
Empowering Anatomic Pathology
Technical Support Manager, StatLab
2090 Commerce| McKinney, TX 75069
t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 
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Re: [Histonet] Derm IHC question

2019-02-08 Thread Cartun, Richard via Histonet 
Doesn't sound like a fixation issue to me.  Could the tissue be drying out 
before it's placed in formalin?  Also, are these specimens inked for assessment 
of margins?  I've seen ink interfere with immunoreactivity.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Debra Siena via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, February 08, 2019 11:51 AM
To: 'histonet'
Subject: [Histonet] Derm IHC question

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Hello fellow Histonetters

I would like to ask you a question about IHC staining and derm cases.  I am 
seeing a peculiar issue going on, where the melanocytes in the middle of the 
tissues are staining pretty well but when you get to the ends of the tissues 
either shaves or ellipses, they are not staining. This is sporadic, not every 
case and there is no consensus as to a common thread between the cases.I 
feel that this may be a fixation issue but was just wondering if anyone had 
ever seen the same phenomena and would be willing to share the theory or even 
better what was the remedy behind this issue.  The fixative is 10% Neutral 
Buffered Formaliln  and the cells in question that are "dropping out" which is 
what the pathologist is describing are melanocytes, especially with Sox-10 and 
Mart 1 antibodies.

Thanks for the assistance, I definitely appreciate it very much.



Best wishes,



[image001]  Debbie Siena, HT(ASCP)QIHC

Empowering Anatomic Pathology

Technical Support Manager, StatLab

2090 Commerce| McKinney, TX 75069

t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369

dsi...@statlab.com<mailto:dsi...@statlab.com>|https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M&s=y7Dkipiv1JdLkqba8XolhtugffjOReKOTWuW0mZaJJI&e=<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M&s=uHeyPcAgHiInkXaGAN29W0BdT9ZKO4f1uuvarGmSNRs&e=>



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[Histonet] Derm IHC question

2019-02-08 Thread Debra Siena via Histonet 
Hello fellow Histonetters
I would like to ask you a question about IHC staining and derm cases.  I am 
seeing a peculiar issue going on, where the melanocytes in the middle of the 
tissues are staining pretty well but when you get to the ends of the tissues 
either shaves or ellipses, they are not staining. This is sporadic, not every 
case and there is no consensus as to a common thread between the cases.I 
feel that this may be a fixation issue but was just wondering if anyone had 
ever seen the same phenomena and would be willing to share the theory or even 
better what was the remedy behind this issue.  The fixative is 10% Neutral 
Buffered Formaliln  and the cells in question that are "dropping out" which is 
what the pathologist is describing are melanocytes, especially with Sox-10 and 
Mart 1 antibodies.
Thanks for the assistance, I definitely appreciate it very much.

Best wishes,

[image001]  Debbie Siena, HT(ASCP)QIHC
Empowering Anatomic Pathology
Technical Support Manager, StatLab
2090 Commerce| McKinney, TX 75069
t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369
dsi...@statlab.com|www.statlab.com

StatLab is an ISO 13485 Certified Company

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