In decalcified sections of bone, yes the osteoid or dense unmineralized
collagen matrix (mostly type I) will stain darker than mature native
demineralized bone. Even though the mature bone has been demineralized, it is
still more densely compact as compared to the newly formed bone that has not
begun mineralization and . In resin embedded undemineralized sections of bone,
the contrast is exactly opposite when staining with Von Kossa and
counterstaining with MacNeal's tetrachrome and similar to decalcified sections
in a Goldner's trichrome where the acid fuchsin stains osteoid darker than the
light green does the mineralized bone. So the answer is yes, tissue density is
what plays the major role in contrast staining and stain intensity.
Jack
On Feb 18, 2011, at 6:45 AM, Keller, Pat kell...@ent.wustl.edu wrote:
We have always noticed, at least in the middle/inner ear, that newly
deposited bone stains darker than mature bone, both with HE and
toluidine blue. Is this increased eosinophilic quality due to a lack of
mineralization and therefore higher density of osteoid components in the
new bone or some other difference in composition of the osteoid? The
contrast is quite striking when we observe bone remodeling due to middle
ear infections, so I wanted to be able to offer an accurate explanation
of why that is...
Patricia Keller
Sr. Research Tech/Core Histologist
Washington University School of Medicine
Department of Otolaryngology
4566 Scott Ave
Campus Box 8115
St. Louis, Mo 63110
314-747-7166
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