AW: [Histonet] FW: Masson's trichrome stain
Maybe I am wrong, but I think the usage of plastic forceps for trichrome stains is nonsense. I only know this from silver stains, but also have never seen problems personally. The polyacids have to stay onto the section, when anilinblue is added. There exists a theory, that PMA/PTA acts like a mordant and builds bridges between collagen and anilinblue. It is proven, that PMA is not washed out of the tissue and is bound to the fibers. On the other hand it seems, that the polyacids prevent the staining of globular proteins by anilinblue (the longer the polyacids the brighter the red colour). It also helps to keep the low staining-pH of anilinblue. Gudrun Lang From: Justine Lanzon [mailto:justinelan...@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamarg...@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: Masson's trichrome stain
The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf lindamarg...@gmail.com wrote: Here is a message from Justine... From: Justine Lanzon [mailto:justinelan...@hotmail.com] justinelan...@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamarg...@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Masson's trichrome stain
Here is a message from Justine... From: Justine Lanzon [mailto:justinelan...@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamarg...@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
