Re: [Histonet] Formalin pH
Yes we are doing the same thing as Brian’s lab. Getting the certificates from the vendor. Emily Horst On Tue, Sep 12, 2023 at 10:17 PM Paula Sicurello via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of > formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating > over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin pH
Thank you Tony, for drawing attention to that excellent 1991 paper in the Journal of Histotechnology. It was published in the years when the NSH's journal was not included in Current Contents and was not taken by most libraries. Sadly, Norman Hew-Shue died in Toronto in 2022. Papers in J. Histotechnol. (including all back-issues) are now easily available as PDF downloads from libraries that subscribe to the many journals published by Taylor and Francis. That's probably most academic and biomedical libraries. Members of the NSH still get the paper journal and have access to the archive by way of an obscure members-only link on the society's web site; it is very difficult to find. Why won't the NSH make its journal conspicuous and easily accessible to all by way of a single click from https://www.nsh.org/home? Grrr! John Kiernan. = = = From: Tony Henwood via Histonet Sent: September 13, 2023 7:01 PM To: Bacon, Charles ; e...@pigs.ag Cc: HistoNet Subject: Re: [Histonet] Formalin pH Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple, when added to formalin solutions, serves as an indicator of pH as well as, from a safety aspect, labelling the solution as formalin making redundant the dangerous “smell” test. At an acidic pH (5.2) the intense indicator colour is yellow whereas at pH 6.8, the colour is purple. A saturated solution of the dye is prepared, and 2 to 4 drops are added to 10 litres of neutral buffered formalin. Other advantages are that the fixative is more readily distinguished from other colourless solutions such as saline, thus preventing accidental misuse, and formalin spills are more easily recognised. Hew-Shue N (1991) “Bromcresol Purple as a Colored Marker and pH Indicator for Ten Percent Neutral Buffered Forrnalin” J Histotechnol 14(4):257-260. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Bacon, Charles via Histonet<mailto:histonet@lists.utsouthwestern.edu> Sent: Thursday, 14 September 2023 6:42 AM To: e...@pigs.ag<mailto:e...@pigs.ag> Cc: HistoNet<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Formalin pH We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "e...@pigs.ag" wrote: > > Sooner or later the people have got to rise up > and tell those brain-dead paper-shuffling regulators > to just stuff it. Their mindless interference is > just wasting time and interfering with people's > ability to keep their own garden. > > -Original Message- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. > That's what we did and now they come with each lot. We do have to bug them > for them occasionally. > > I'm betting your vendor has at least heard about this from other customers so > it should be on their radar at the very least. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> > > ________ > From: Paula Sicurello via Histonet > Sent: Tuesday, September 12, 2023 7:12:05 PM > To: HistoNet > Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* > This email came from outside CHLA. Do not open attachments, click on links, > or respond unless you expected this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of > formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating > over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2a
Re: [Histonet] Formalin pH
Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple, when added to formalin solutions, serves as an indicator of pH as well as, from a safety aspect, labelling the solution as formalin making redundant the dangerous “smell” test. At an acidic pH (5.2) the intense indicator colour is yellow whereas at pH 6.8, the colour is purple. A saturated solution of the dye is prepared, and 2 to 4 drops are added to 10 litres of neutral buffered formalin. Other advantages are that the fixative is more readily distinguished from other colourless solutions such as saline, thus preventing accidental misuse, and formalin spills are more easily recognised. Hew-Shue N (1991) “Bromcresol Purple as a Colored Marker and pH Indicator for Ten Percent Neutral Buffered Forrnalin” J Histotechnol 14(4):257-260. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Bacon, Charles via Histonet<mailto:histonet@lists.utsouthwestern.edu> Sent: Thursday, 14 September 2023 6:42 AM To: e...@pigs.ag<mailto:e...@pigs.ag> Cc: HistoNet<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Formalin pH We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "e...@pigs.ag" wrote: > > Sooner or later the people have got to rise up > and tell those brain-dead paper-shuffling regulators > to just stuff it. Their mindless interference is > just wasting time and interfering with people's > ability to keep their own garden. > > -Original Message- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. > That's what we did and now they come with each lot. We do have to bug them > for them occasionally. > > I'm betting your vendor has at least heard about this from other customers so > it should be on their radar at the very least. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> > > ________ > From: Paula Sicurello via Histonet > Sent: Tuesday, September 12, 2023 7:12:05 PM > To: HistoNet > Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* > This email came from outside CHLA. Do not open attachments, click on links, > or respond unless you expected this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of > formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating > over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$<https://urldefense.com/v3/__http:/secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8c
Re: [Histonet] Formalin pH
We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "e...@pigs.ag" wrote: > > Sooner or later the people have got to rise up > and tell those brain-dead paper-shuffling regulators > to just stuff it. Their mindless interference is > just wasting time and interfering with people's > ability to keep their own garden. > > -Original Message- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. > That's what we did and now they come with each lot. We do have to bug them > for them occasionally. > > I'm betting your vendor has at least heard about this from other customers so > it should be on their radar at the very least. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> > > ________ > From: Paula Sicurello via Histonet > Sent: Tuesday, September 12, 2023 7:12:05 PM > To: HistoNet > Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* > This email came from outside CHLA. Do not open attachments, click on links, > or respond unless you expected this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of > formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating > over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$ > > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential or > legally privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePelpt2pjY$ > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin pH
Sooner or later the people have got to rise up and tell those brain-dead paper-shuffling regulators to just stuff it. Their mindless interference is just wasting time and interfering with people's ability to keep their own garden. -Original Message- From: Cooper, Brian via Histonet Reply-To: Cooper, Brian To: Paula Sicurello, HistoNet Subject: Re: [Histonet] Formalin pH Date: Today 10:19 AM Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> From: Paula Sicurello via Histonet Sent: Tuesday, September 12, 2023 7:12:05 PM To: HistoNet Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin pH
You can check the pH with a pH meter. This is the most accurate way, but the meter's electrode must be calibrated against at least two standard (usually bought) buffer solutions, such as 4.0 and 7.0. If you don't have a pH meter or the know-how to use one properly, you can use indicator papers, which are inexpensive but deteriorate with storage. With a few "books" of indicator papers covering the range 3 to 8 you should be able to get a value accurate to the nearest 0.2 pH units. I'm assuming that by "formalin" you mean the concentrated solution that is nominally 37-40% by weight of formaldehyde gas in water. Its pH falls with time. For more information about formalin, see https://www.cambridge.org/core/services/aop-cambridge-core/content/view/911F1870567E2487D56A51140EB8EA17/S1551929500057060a.pdf/div-class-title-formaldehyde-formalin-paraformaldehyde-and-glutaraldehyde-what-they-are-and-what-they-do-div.pdf. <https://www.cambridge.org/core/services/aop-cambridge-core/content/view/911F1870567E2487D56A51140EB8EA17/S1551929500057060a.pdf/div-class-title-formaldehyde-formalin-paraformaldehyde-and-glutaraldehyde-what-they-are-and-what-they-do-div.pdf> It's a free download, with >600 citations according to Google Scholar. John Kiernan Emeritus, UWO, London, Canada = = = From: Paula Sicurello via Histonet Sent: September 12, 2023 10:11 PM To: HistoNet Subject: [Histonet] Formalin pH Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin pH
Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> From: Paula Sicurello via Histonet Sent: Tuesday, September 12, 2023 7:12:05 PM To: HistoNet Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin pH
Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
