[Histonet] Formula 83: xylene substitute
Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83: xylene substitute
Kiran, We use Formula 83 on both staining and processing without any problem at all. You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene. We also use CBG's recycler to recycle Formula 83 without any problem. We have not had any problems with our IHC using Formula 83 either. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiran Sent: Tuesday, May 14, 2013 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83: xylene substitute Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83: xylene substitute
Thanks, Linda. What about the odor? Does it have strong or irritating odor? How did you staff responded to the change? We would like to know all before trying it out. Currenly, our staff is having issues with Xylene fumes and goal is to limit any fumes or exposure to Xylene. I would like to hear from others who tried to use it and didn't work for them. -Kiran --- On Tue, 5/14/13, Blazek, Linda lbla...@digestivespecialists.com wrote: From: Blazek, Linda lbla...@digestivespecialists.com Subject: RE: [Histonet] Formula 83: xylene substitute To: Kiran kira...@sbcglobal.net, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, May 14, 2013, 7:13 AM Kiran, We use Formula 83 on both staining and processing without any problem at all. You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene. We also use CBG's recycler to recycle Formula 83 without any problem. We have not had any problems with our IHC using Formula 83 either. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiran Sent: Tuesday, May 14, 2013 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83: xylene substitute Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83
We have 2 cases of CBG's Formula 83. We would like to find it a home instead of dumping it as hazardous waste. Our lab is closing so we can no longer use it. If you would like it and are willing to set up and pay for shipping it is yours. Cindy DuBois Integrated Pathology Stockton, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83
Mr. Richmond, I was contacted off site and asked to clarify something you posted to the Histonet. I hope you do not mind. The flashpoint of Formula 83 is not 7 degrees Fahrenheit. It is 7 degrees Celsius, or 45 degrees Fahrenheit. Also please note that the auto ignite temperature is 482 degrees Fahrenheit. Respectfully, Beth Austin-Sell CBG Biotech -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: GI, Uro, or Derm Path Lab Set Up (Sally Price) 2. Formula 83 (Robert Richmond) 3. RE: cleaning cryostat (Ingles Claire ) 4. What slides do you use? (Scott Hendricksen) 5. New slide drier recommendations please (Scott Hendricksen) 6. Re: BioCare immunostainer (Pat Laurie) 7. Slide baking before IHC (Pat Laurie) 8. Re: RE: GI, Uro, or Derm Path Lab Set Up (Jay Lundgren) 9. Temperature and Humidity Monitoring System (teri.hall...@midmichigan.org) 10. RE: Slide baking before IHC (Morken, Tim) 11. RE: Temperature and Humidity Monitoring System (Liz Chlipala) -- Message: 1 Date: Thu, 4 Feb 2010 19:48:23 -0500 From: Sally Price sprice2...@gmail.com Subject: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up To: histonet@lists.utsouthwestern.edu Message-ID: ffd26fa81002041648y5e648596sc4034bf7e8437...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 ...and another thing -- since when is it OK to post such blatant solicitations on the HistoNet? other vendors are chastised for this on a regular basis! Sally -- Message: 2 Date: Thu, 4 Feb 2010 21:14:50 -0500 From: Robert Richmond rsrichm...@gmail.com Subject: [Histonet] Formula 83 To: Histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: abea52a61002041814g4e82823dx50ebc41d77dd8...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Allison Hutton, HTL(ASCP)cm, Lead Tech Histology, Doylestown Hospital, Doylestown, Pennsylvania asks: For those of you who are using Formula 83, could you please contact me off line? We have completed our demo but still have a few questions. Formula 83, offered by CBG Biotech Ltd., is a napthenic (cycloalkane) hydrocarbon used as a xylene substitute. It differs in structure from the aliphatic hydrocarbons such as Richard Allan's Clear-rite 3. It's in use in a lab I'm working in at the moment. I have two reservations about it. The first is that, unlike the aliphatics, it has a definite odor, which could be objectionable in a confined space. The second reservation: Formula 83 has a flash point of only 7 degrees Fahrenheit, lower than xylene (78 degrees) and other aliphatics, some of which have flash points as high as 144 F. The fire hazard here is worth considering. Formula 83 has been discussed before on Histonet, and the posts are worth looking up. When purchasing a new aliphatic, there are several important considerations. Your purchasing people need to understand that they can't change the xylene substitute on you, just because something a bit cheaper comes along. If you recycle, every different xylene substitute has a different distillation routine. It's important to ask everyone in the lab - in particular, the cytotechnologist is likely to have very strong opinions about the solvents they use in their ascending sequence before coverslipping. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Thu, 4 Feb 2010 22:44:24 -0600 From: Ingles Claire cing...@uwhealth.org Subject: RE: [Histonet] cleaning cryostat To: histonet@lists.utsouthwestern.edu Message-ID: f2f030053f9b7345831bed293a6d57e109a...@uwhc-mail01.uwhis.hosp.wisc.edu Content-Type: text/plain;charset=iso-8859-1 I work in a Mohs Lab. (high output frozen skin) We wipe out the cryostat with 95% ETOH and clear out the shavings every day we use our machines. We normally tear down and completely defrost our cryostats every other month or so, depending on humidity
[Histonet] Formula 83
Allison Hutton, HTL(ASCP)cm, Lead Tech Histology, Doylestown Hospital, Doylestown, Pennsylvania asks: For those of you who are using Formula 83, could you please contact me off line? We have completed our demo but still have a few questions. Formula 83, offered by CBG Biotech Ltd., is a napthenic (cycloalkane) hydrocarbon used as a xylene substitute. It differs in structure from the aliphatic hydrocarbons such as Richard Allan's Clear-rite 3. It's in use in a lab I'm working in at the moment. I have two reservations about it. The first is that, unlike the aliphatics, it has a definite odor, which could be objectionable in a confined space. The second reservation: Formula 83 has a flash point of only 7 degrees Fahrenheit, lower than xylene (78 degrees) and other aliphatics, some of which have flash points as high as 144 F. The fire hazard here is worth considering. Formula 83 has been discussed before on Histonet, and the posts are worth looking up. When purchasing a new aliphatic, there are several important considerations. Your purchasing people need to understand that they can't change the xylene substitute on you, just because something a bit cheaper comes along. If you recycle, every different xylene substitute has a different distillation routine. It's important to ask everyone in the lab - in particular, the cytotechnologist is likely to have very strong opinions about the solvents they use in their ascending sequence before coverslipping. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83
For those of you who are using Formula 83, could you please contact me off line. We have completed our demo but still have a few questions. Thanks in advance, Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital 595 W. State St Doylestown, PA 18901 215-345-2264 ahut...@dh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83
Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83
Phyllis, I have used Formula 83 for several years and have no problem with it. I do use it just like xylene. As for how frequently you need to change it on the processor really depends on your volume of processing. I didn't have to change the frequency when I switched from xylene to Formula 83. I also recycle my Formula 83. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Wednesday, July 22, 2009 10:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83 Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formula 83 users?
Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. René J. --- On Wed, 4/8/09, Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca wrote: From: Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca Subject: [Histonet] Formula 83 users? To: Histonet Histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83 users?
With all due respect René; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 09, 2009 9:56 AM To: Histonet; Jacqueline Farnsworth Subject: Re: [Histonet] Formula 83 users? Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. René J. --- On Wed, 4/8/09, Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca wrote: From: Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca Subject: [Histonet] Formula 83 users? To: Histonet Histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83-Thank you!
Thank you all for taking time to respond to my inquiry about Formula 83. We received a free sample and will perform some trials and I will post our results on the Histonet asap. Of course, there can always be hidden challenges bringing reagents over the Canadian border, so that in itself can prove interesting, (eh!) Thanks Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83 users?
HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83 users?
I can't speak to its functionality, but per its MSDS it is extremely flammable (FP= 45F) and would, thus be considered a hazardous waste by the EPA.EPA's regulations for all solid hazardous waste (which includes liquids) produced by healthcare facilities and others require that they be handled (including monitoring, storage, and disposal) in accordance with EPA's requirements, and specifically state that hazardous wastes generated in the (healthcare) laboratory cannot be disposed into drains. Since Micro-ClearT is not classified as hazardous by EPA, it is drain-disposable under its regulations. Peter Peter G. Baldwin Director of Sales, Marketing Business Development pbald...@micronenvironmental.com Micron Environmental Industries, Inc. Green Chemistry for LifeSM www.MicronEnvironmental.com 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
