RE: [Histonet] Gomori Trichrome
Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gomori Trichrome
Joanna, I agree with Tim... We also see this at times if the tissue has freezing artifact. Gomori Trichrome is very unforgiving :) Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Thursday, December 11, 2014 11:19 AM To: 'Joanna'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gomori Trichrome Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v2/url?u=http-3A__mailto-3Ahistonet-2Dbounces-40lists.utsouthwestern.edud=AAIFAgc=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8r=7bt8tbqem5cRuX3hC3eOeQm=0vo4hfofYzOL04nyQnusz7sZCdl6SlMBYQxPhvKuLTos=9bOOBwtwMikwEx-4zwlWv08DLvU_t-ipNcKOpMi5GfAe= ] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=AAIFAgc=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8r=7bt8tbqem5cRuX3hC3eOeQm=0vo4hfofYzOL04nyQnusz7sZCdl6SlMBYQxPhvKuLTos=j_zGkNIiR0dF7a9QsR9pyFxqkZn_Q4JNpgF8pigo2UUe= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gomori Trichrome
Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Gomori Trichrome Staining
Hi, you can try, if you do not already, to wash briefly in a solution of 0.2 ml. of glacial acetic acid in 100 ml. of distilled water, before the final dehydration. That makes the colors more delicate and transparent. The Gomori's trichromic results are similar to those obtained with the Masson's trichrome staining: Cytoplasms from bright red to greenish. Kind Regards Massimo Tosi A humble Chemical Engineer who loves Histology Da: Eleonora Guadagnin guadagnin.eleon...@gmail.com A: histonet@lists.utsouthwestern.edu Inviato: Martedì 14 Maggio 2013 21:33 Oggetto: [Histonet] Gomori Trichrome Staining Hello everybody, I am performing the Gomori Trichrome staining on frozen muscle sections (10um) but I have couple of issues: 1. The green staining is TOO dark; 2. When the green is not too dark, all my muscle fibers are red. I checked the pH of the Gomori solution, is 2.15. If anyone has some advice, please Thanks a lot! Eleonora ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gomori Trichrome Staining
Hello everybody, I am performing the Gomori Trichrome staining on frozen muscle sections (10um) but I have couple of issues: 1. The green staining is TOO dark; 2. When the green is not too dark, all my muscle fibers are red. I checked the pH of the Gomori solution, is 2.15. If anyone has some advice, please Thanks a lot! Eleonora ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gomori Trichrome and nemaline bodies?
Hello All, I work in a core facility providing laboratory services to researchers. I had a request to cut some frozen sections on muscle tissue, and stain them with Gomori Trichrome, a technique we use routinely. I did the stain, checked them microscopically andconfirmed that the collagen fibers were nicely stained. But the researcher came back and said that the slides did not show what she was looking for, which was nemaline bodies, rod-shaped structures indicative of a disease called nemaline myopathy. I had never heard of this but went online and found several articles on the disease, a couple of which showed images of nemaline bodies stained with a modified Gomori Trichrome procedure. But no indications of what the modifications were. Is anyone familiar with the staining of these bodies, either using a modified Gomori trichrome, or some other method? Thanks. Paul Monfils ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
