[Histonet] H Pylori IHC

[Rapp, Keith via Histonet]

Hello, we have just purchased the Leica Bond Max IHC stainer and love it. It's 
easy to use and produces beautiful stains.
However, Helico Pylori has been giving us problems with weak and incomplete 
staining (not all of the organisms staining).
Is there anyone using the Bond Max and successfully staining H. Pylori?
 We have tried two different antibody vendors, Leica and Biocare Medical giving 
similar results. We have also tried several different Antigen Retrieval methods.

Thank you,

Keith Rapp
Histology Technical Specialist
Rady Children's Hospital
Department of Pathology
kr...@rchsd.org
858-966-1700 x225181

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Re: [Histonet] H Pylori controls

[Mca Werdler via Histonet]

Unsubscribe

Op vr 22 jan. 2021 9:53 p.m. schreef Hans B Snyder via Histonet <
histonet@lists.utsouthwestern.edu>:

> Hello Cynthia,
>
> We have HP blocks.  Our tissues are great for IHC but not special stains.
> Please contact me directly if you are interested.
>
> Thank you
>
> Hans B Snyder
> JMD Histology & Histologistics Inc.
> 118 Main St, Store Front, Suite 1
> Webster, MA 01570
> Lab - 508-461-7207
> www.histologistics.com
> http://jmdhistology.com/
> -To Err is
> Human---
>
> The information contained in this message and any attachments is intended
> only
> for the use of the individual or entity to which it is addressed, and may
> contain
> information that is privileged, confidential and exempt from disclosure
> under
> applicable law. If you have received this message in error, you are
> prohibited
> from copying, distributing or using the information. Please contact the
> sender
> immediately by return e-mail and delete the original message.
>
>
> On Fri, Jan 22, 2021 at 1:12 PM  >
> wrote:
>
> > Send Histonet mailing list submissions to
> > histonet@lists.utsouthwestern.edu
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
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> > histonet-ow...@lists.utsouthwestern.edu
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Histonet digest..."
> >
> >
> > Today's Topics:
> >
> >1. H Pylori controls (Cynthia McGrady)
> >2. Service provider for growing fungal strains? (Craig)
> >3. Embedding (Ann Specian)
> >    4. water under sectios (Bernice Frederick)
> >
> >
> > --
> >
> > Message: 1
> > Date: Thu, 21 Jan 2021 13:21:14 -0500
> > From: Cynthia McGrady 
> > To: Histonet 
> > Subject: [Histonet] H Pylori controls
> > Message-ID:
> > <
> > cafn2hshezlsrd-8brzj+8ezknn3zxvoglz93+bgmov820sz...@mail.gmail.com>
> > Content-Type: text/plain; charset="UTF-8"
> >
> > Hello Histonetters.
> > Looking for positive H Pylori control block(s). We typically use
> > positive case tissue from old surgical cases, but seem to be running low.
> > If anyone has a surplus, and would be willing to share, or maybe we have
> > tissue you can use?? We could do a swap..Let me know..Thanks...Cindy
> > McGrady
> >
> > Cindy McGrady
> > HT(ASCP) QIHC
> > Hurley Medical Center
> > One Hurley Plaza
> > Flint, Mi 48503
> > 810-262-9138
> >
> > --
> >
> >
> > If you are not the intended
> > recipient(s), you are notified that any
> > disclosure, copying, distribution or
> > any action taken or omitted to be
> > taken in reliance on the contents of this
> > information is prohibited and may
> > be unlawful. If you receive this message in
> > error, or are not the named
> > recipient(s), please notify the sender, delete this
> > e-mail from your
> > computer, and destroy any copies in any form immediately.
> >
> >
> > --
> >
> > Message: 2
> > Date: Thu, 21 Jan 2021 12:35:25 -0600
> > From: Craig 
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Service provider for growing fungal strains?
> > Message-ID:
> >  > 9lyz12axoa67mv0cdfak1v0n5cmxnmfyu90vydfe7...@mail.gmail.com>
> > Content-Type: text/plain; charset="UTF-8"
> >
> > Hello all!
> >
> > Does anyone know a lab that can grow specific fungal strains?
> >
> > We are looking to make internal FFPE control blocks for specific fungal
> > strains. We have an internal method for producing FFPE blocks. Just need
> a
> > lab or service provider that can grow specific fungal cultures and send
> us
> > the fixed bugs.
> >
> > Any suggestions would super grateful!
> >
> > Best,
> > Craig
> > volle...@gmail.com
> > cr...@champdx.com
> > ?
> >
> >
> > --
> >
> > Message: 3
> > Date: Fri, 22 Jan 2021 15:43:26 + (UTC)
> > From: Ann Specian 
> > To: "histonet@lists.utsouthwestern.edu"
&g

[Histonet] H Pylori controls

[Hans B Snyder via Histonet]

Hello Cynthia,

We have HP blocks.  Our tissues are great for IHC but not special stains.
Please contact me directly if you are interested.

Thank you

Hans B Snyder
JMD Histology & Histologistics Inc.
118 Main St, Store Front, Suite 1
Webster, MA 01570
Lab - 508-461-7207
www.histologistics.com
http://jmdhistology.com/
-To Err is
Human---

The information contained in this message and any attachments is intended
only
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information that is privileged, confidential and exempt from disclosure
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prohibited
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sender
immediately by return e-mail and delete the original message.


On Fri, Jan 22, 2021 at 1:12 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. H Pylori controls (Cynthia McGrady)
>2. Service provider for growing fungal strains? (Craig)
>3. Embedding (Ann Specian)
>4. water under sectios (Bernice Frederick)
>
>
> --
>
> Message: 1
> Date: Thu, 21 Jan 2021 13:21:14 -0500
> From: Cynthia McGrady 
> To: Histonet 
> Subject: [Histonet] H Pylori controls
> Message-ID:
> <
> cafn2hshezlsrd-8brzj+8ezknn3zxvoglz93+bgmov820sz...@mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hello Histonetters.
> Looking for positive H Pylori control block(s). We typically use
> positive case tissue from old surgical cases, but seem to be running low.
> If anyone has a surplus, and would be willing to share, or maybe we have
> tissue you can use?? We could do a swap..Let me know..Thanks...Cindy
> McGrady
>
> Cindy McGrady
> HT(ASCP) QIHC
> Hurley Medical Center
> One Hurley Plaza
> Flint, Mi 48503
> 810-262-9138
>
> --
>
>
> If you are not the intended
> recipient(s), you are notified that any
> disclosure, copying, distribution or
> any action taken or omitted to be
> taken in reliance on the contents of this
> information is prohibited and may
> be unlawful. If you receive this message in
> error, or are not the named
> recipient(s), please notify the sender, delete this
> e-mail from your
> computer, and destroy any copies in any form immediately.
>
>
> --
>
> Message: 2
> Date: Thu, 21 Jan 2021 12:35:25 -0600
> From: Craig 
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Service provider for growing fungal strains?
> Message-ID:
>  9lyz12axoa67mv0cdfak1v0n5cmxnmfyu90vydfe7...@mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hello all!
>
> Does anyone know a lab that can grow specific fungal strains?
>
> We are looking to make internal FFPE control blocks for specific fungal
> strains. We have an internal method for producing FFPE blocks. Just need a
> lab or service provider that can grow specific fungal cultures and send us
> the fixed bugs.
>
> Any suggestions would super grateful!
>
> Best,
> Craig
> volle...@gmail.com
> cr...@champdx.com
> ?
>
>
> --
>
> Message: 3
> Date: Fri, 22 Jan 2021 15:43:26 + (UTC)
> From: Ann Specian 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Embedding
> Message-ID: <655838156.2928090.1611330206...@mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> Is anyone aware of any NYS/CAP/CLIA regulations regarding credentialing
> required to perform embedding in NJ?Thank you, Ann
>
> --
>
> Message: 4
> Date: Fri, 22 Jan 2021 17:15:40 +
> From: Bernice Frederick 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] water under sectios
> Message-ID:
> <
> mwhpr05mb294386def0662af11f9ff66c8f...@mwhpr05mb2943.namprd05.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good morning (well, in Chicago anywa

[Histonet] H Pylori controls

[Cynthia McGrady via Histonet]

Hello Histonetters.
Looking for positive H Pylori control block(s). We typically use
positive case tissue from old surgical cases, but seem to be running low.
If anyone has a surplus, and would be willing to share, or maybe we have
tissue you can use?? We could do a swap..Let me know..Thanks...Cindy McGrady

Cindy McGrady
HT(ASCP) QIHC
Hurley Medical Center
One Hurley Plaza
Flint, Mi 48503
810-262-9138

-- 


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[Histonet] H. pylori stain

[Amy Johnson via Histonet]

Hello histoland

We currently use Ventana's h. pylori antibody on our Benchmark ultra.  Over the 
last few months our pathologists have been noticing "junk" that is staining 
with this antibody.  It seems to be on the tissue more than in the tissue and 
its not on all slides.  WE have tried changing our waterbath from tap water to 
distilled water with no difference in staining.  We thought maybe our paraffin 
was bad so we stained a "blank" block of paraffin but could not find the 
culprit...Any insight would be appreciated!

Thanks
Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401
715-847-2130

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[Histonet] H Pylori control blocks

[Mike Pence via Histonet]

Hey Histo world-
Does anyone have any blocks to spare that are + for H Pylori? I could sure use 
a couple for IHC.

Michael S. Pence | Supervisor of Laboratory Services
Great River Health Systems
1221 S. Gear Ave. | West Burlington, IA 52655
Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
mpe...@grhs.net | 
www.greatrivermedical.org.
www.Facebook.com/GreatRiverHealthSystems
 | www.Twitter/GreatRiverMed


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the sender and delete this communication from your device.



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[Histonet] h. pylori

[Dooley, Elaine via Histonet]

Hi histonetters,

We filter the H.pylori antibody with the filters that come with the Ventana 
Prep kits.

Elaine Dooley
Shands Teaching Hospital
352-265-0111 ext 72117
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[Histonet] H. pylori by IHC

[Joanne Clark via Histonet]

Recently someone placed a query regarding the precipitate with Cell Marques H. 
pylori marker.  The response was to filter it before use to clean it up.  May I 
ask how it is being filtered?  We have the same problem and it makes the slide 
difficult to interpret.


Joanne Clark, HT BAAS
Director of Histology
Pathology Consultants of New Mexico



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[Histonet] h. pylori

[Dooley, Elaine via Histonet]

Hi,

We filter our H.Pylori antibody from Cell Marque before using it on our Ventana 
Ultra and XTs and this has made it a cleaner stain.

Elaine Dooley
Shands Teaching Hospital
Gainesville FL
352-265-0111   ext 72117
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[Histonet] H. pylori control tissue

[Cartun, Richard via Histonet]

Nancy Schmitt from Dubuque, IA asked about control tissue for H. pylori.  I 
have tried e-mailing her, but my e-mail was returned as "Undeliverable".  
Nancy, please contact me.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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[Histonet] H. Pylori

[Nancy Schmitt via Histonet]

Good Morning-

I am looking for H. Pylori control - does anyone have any extra lying about?  
What are you currently using?  Purchasing?
I appreciate any and all feedback!

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001
Check us out at www.uclaccess.com





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[Histonet] H. Pylori Testing

[Amos Brooks]

Hi,
 If you are looking at the possibility of doing IHC manually, I would
really recommend the Shandon Sequenza. It is a box that holds clips that
attach to your slides. The slides are held vertically and you drop reagents
into the top. The reagents replace the previous reagent which drips into a
catch tray below. It makes it **really** easy to reproducibly do IHC
manually.

Amos Brooks


On Tue, Apr 28, 2015 at 12:24 PM, histonet-requ...@lists.utsouthwestern.edu
 wrote:

 Message: 8
 Date: Mon, 27 Apr 2015 16:49:25 -0400
 From: Garreyf garr...@gmail.com
 Subject: Re: [Histonet] H. Pylori Testing
 To: Michael Ann Jones mjo...@metropath.com
 Cc: histonet@lists.utsouthwestern.edu
 histonet@lists.utsouthwestern.edu,Vickroy, James
 jvick...@springfieldclinic.com
 Message-ID: e23744e7-8ab0-4d32-a3bf-cc0b7c656...@gmail.com
 Content-Type: text/plain;   charset=us-ascii

 Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris
 in house as well. I'm trying to create more revenue to support a 2nd
 histotech.

 Garrey

 Sent from my iPhone

  On Apr 27, 2015, at 4:11 PM, Michael Ann Jones mjo...@metropath.com
 wrote:
 
  We recently switched to IHC stain for HP, however, before that we used
  Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
  Cell Marque by hand (with great results) and now we are automated.
 
  Michael Ann
  Michael Ann Jones, HT (ASCP)
  Histology Manager
  Metropath
  7444 W. Alaska Dr. #250
  Lakewood, CO 80226
  303.634.2511
  mjo...@metropath.com

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Re[2]: [Histonet] H. Pylori Testing

[Brendal Finlay]

We do HP and Mart-1 testing in house by hand using concentrates.  It's a pretty 
basic set up using a pressure cooker, stain tray, pap pen, and the reagents. At 
this point in time it's less expensive than automation.  We start them around 
10 am and finish around 12 or 1 pm.  

  
Brendal C. Finlay, HT (ASCP)
Senior Histologist
Medical Center Clinic
8333 North Davis Highway
Pensacola, FL 32514
Phone 850.474.8581
Fax 850.474.8584 

-Original Message- 
From: Garreyf garr...@gmail.com 
To: Michael Ann Jones mjo...@metropath.com 
Cc: histonet@lists.utsouthwestern.edu, Vickroy, James 
jvick...@springfieldclinic.com 
Date: 04/27/2015 15:49 
Subject: Re: [Histonet] H. Pylori Testing 

Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris in 
house as well. I'm trying to create more revenue to support a 2nd histotech.

Garrey

Sent from my iPhone

 On Apr 27, 2015, at 4:11 PM, Michael Ann Jones mjo...@metropath.com wrote:
 
 We recently switched to IHC stain for HP, however, before that we used
 Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
 Cell Marque by hand (with great results) and now we are automated.
 
 Michael Ann
 Michael Ann Jones, HT (ASCP)
 Histology Manager
 Metropath
 7444 W. Alaska Dr. #250
 Lakewood, CO 80226
 303.634.2511
 mjo...@metropath.com
 
 
 
 
 
 
 On 4/27/15, 2:04 PM, Vickroy, James jvick...@springfieldclinic.com
 wrote:
 
 
 We are a small lab processing mostly GI specimens.  Currently we are
 sending our H. Pylori testing to a local hospital for staining however I
 can predict that this year we may have around 1000 H. Pylori stains done.
 I am looking for a small platform or manual test kit to perform the H.
 Pylori's inhouse.   Can someone please give me their thoughts,
 suggestions, or similar experiences  how they have or would approach this
 endeavor?
 
 Jim Vickroy
 Histology Manager
 Springfield Clinic, Main Campus, East Building
 1025 South 6th Street
 Springfield, Illinois  62703
 Office:  217-528-7541, Ext. 15121
 Email:  
 jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com
 
 
 
 This electronic message contains information from Springfield Clinic, LLP
 that may be confidential, privileged, and/or sensitive. This information
 is intended for the use of the individual(s) or entity(ies) named above.
 If you are not the intended recipient, be aware that disclosure, copying,
 distribution, or action taken on the contents of this information is
 strictly prohibited. If you have received this electronic message in
 error, please notify the sender immediately, by electronic mail, so that
 arrangements may be made for the retrieval of this electronic message.
 Thank you.___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 ___
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Re: [Histonet] H. Pylori Testing

[Michael Ann Jones]

We recently switched to IHC stain for HP, however, before that we used
Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
Cell Marque by hand (with great results) and now we are automated.

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 4/27/15, 2:04 PM, Vickroy, James jvick...@springfieldclinic.com
wrote:


We are a small lab processing mostly GI specimens.  Currently we are
sending our H. Pylori testing to a local hospital for staining however I
can predict that this year we may have around 1000 H. Pylori stains done.
 I am looking for a small platform or manual test kit to perform the H.
Pylori's inhouse.   Can someone please give me their thoughts,
suggestions, or similar experiences  how they have or would approach this
endeavor?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  
jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP
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is intended for the use of the individual(s) or entity(ies) named above.
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strictly prohibited. If you have received this electronic message in
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Re: [Histonet] H. Pylori Testing

[Garreyf]

Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris in 
house as well. I'm trying to create more revenue to support a 2nd histotech.

Garrey

Sent from my iPhone

 On Apr 27, 2015, at 4:11 PM, Michael Ann Jones mjo...@metropath.com wrote:
 
 We recently switched to IHC stain for HP, however, before that we used
 Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
 Cell Marque by hand (with great results) and now we are automated.
 
 Michael Ann
 Michael Ann Jones, HT (ASCP)
 Histology Manager
 Metropath
 7444 W. Alaska Dr. #250
 Lakewood, CO 80226
 303.634.2511
 mjo...@metropath.com
 
 
 
 
 
 
 On 4/27/15, 2:04 PM, Vickroy, James jvick...@springfieldclinic.com
 wrote:
 
 
 We are a small lab processing mostly GI specimens.  Currently we are
 sending our H. Pylori testing to a local hospital for staining however I
 can predict that this year we may have around 1000 H. Pylori stains done.
 I am looking for a small platform or manual test kit to perform the H.
 Pylori's inhouse.   Can someone please give me their thoughts,
 suggestions, or similar experiences  how they have or would approach this
 endeavor?
 
 Jim Vickroy
 Histology Manager
 Springfield Clinic, Main Campus, East Building
 1025 South 6th Street
 Springfield, Illinois  62703
 Office:  217-528-7541, Ext. 15121
 Email:  
 jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com
 
 
 
 This electronic message contains information from Springfield Clinic, LLP
 that may be confidential, privileged, and/or sensitive. This information
 is intended for the use of the individual(s) or entity(ies) named above.
 If you are not the intended recipient, be aware that disclosure, copying,
 distribution, or action taken on the contents of this information is
 strictly prohibited. If you have received this electronic message in
 error, please notify the sender immediately, by electronic mail, so that
 arrangements may be made for the retrieval of this electronic message.
 Thank you.___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
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Re: [Histonet] H. Pylori Testing

[Michael Ann Jones]

If you do it by IHC it is the normal 2 hour or so process step-by-step.
Giemsa is quick 15 minutes or around there? Again, not as sensitive but
works.

Michael Ann




On 4/27/15, 2:49 PM, Garreyf garr...@gmail.com wrote:

Is it a pain in the neck to do it  by hand? I'd like to bring my h
pyloris in house as well. I'm trying to create more revenue to support a
2nd histotech.

Garrey

Sent from my iPhone

 On Apr 27, 2015, at 4:11 PM, Michael Ann Jones mjo...@metropath.com
wrote:
 
 We recently switched to IHC stain for HP, however, before that we used
 Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
 Cell Marque by hand (with great results) and now we are automated.
 
 Michael Ann
 Michael Ann Jones, HT (ASCP)
 Histology Manager
 Metropath
 7444 W. Alaska Dr. #250
 Lakewood, CO 80226
 303.634.2511
 mjo...@metropath.com
 
 
 
 
 
 
 On 4/27/15, 2:04 PM, Vickroy, James jvick...@springfieldclinic.com
 wrote:
 
 
 We are a small lab processing mostly GI specimens.  Currently we are
 sending our H. Pylori testing to a local hospital for staining however
I
 can predict that this year we may have around 1000 H. Pylori stains
done.
 I am looking for a small platform or manual test kit to perform the H.
 Pylori's inhouse.   Can someone please give me their thoughts,
 suggestions, or similar experiences  how they have or would approach
this
 endeavor?
 
 Jim Vickroy
 Histology Manager
 Springfield Clinic, Main Campus, East Building
 1025 South 6th Street
 Springfield, Illinois  62703
 Office:  217-528-7541, Ext. 15121
 Email:  
 jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com
 
 
 
 This electronic message contains information from Springfield Clinic,
LLP
 that may be confidential, privileged, and/or sensitive. This
information
 is intended for the use of the individual(s) or entity(ies) named
above.
 If you are not the intended recipient, be aware that disclosure,
copying,
 distribution, or action taken on the contents of this information is
 strictly prohibited. If you have received this electronic message in
 error, please notify the sender immediately, by electronic mail, so
that
 arrangements may be made for the retrieval of this electronic message.
 Thank you.___
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
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[Histonet] H. Pylori Testing

[Vickroy, James]

We are a small lab processing mostly GI specimens.  Currently we are sending 
our H. Pylori testing to a local hospital for staining however I can predict 
that this year we may have around 1000 H. Pylori stains done.  I am looking for 
a small platform or manual test kit to perform the H. Pylori's inhouse.   Can 
someone please give me their thoughts, suggestions, or similar experiences  how 
they have or would approach this endeavor?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
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[Histonet] H. pylori

[Abbott, Tanya]

Can anyone recommend a good commercially bought slide for H.pylori?
Thanks!  Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

This electronic mail and any attached documents are intended solely for the 
named addressee(s) and contain confidential information. If you are not an 
addressee, or responsible for delivering this email to an addressee, you have 
received this email in error and are notified that reading, copying, or 
disclosing this email is prohibited. If you received this email in error, 
immediately reply to the sender and delete the message completely from your 
computer system.
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Re: [Histonet] H pylori and Ventana instruments

[Roger Heyna]

We use Ventana's HP antibody, and we do see some of the small, non-specific 
globs of stain, but the pathologists are comfortable differentiating between 
the true and false staining since they are familiar with this artefact.
 
Roger Heyna

 Rae Staskiewicz raest...@grics.net 5/7/2013 12:56 PM 
Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori 
antibody you are using, and whether or not you are experiencing non-specific 
staining, or staining that looks specific but with a stippled effect. 

Thanks

Rae Staskiewicz
UnityPoint Health-Methodist


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RE: [Histonet] H pylori and Ventana instruments

[Truscott, Tom]

I haven't used HP, but have noticed this stippled effect especially when using 
40X on the microscope and looking at slides labeled with an Ventana alk-phos 
kit. I seem to have solved the problem by making sure no trace of H2O is left 
on the slides. It helps after washing slides to run them thru a few changes of 
alcohol then one acetone and then a couple xylene before coverslipping. Ventana 
suggested the acetone step. Tom T

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna
Sent: Wednesday, May 08, 2013 8:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] H pylori and Ventana instruments

We use Ventana's HP antibody, and we do see some of the small, non-specific 
globs of stain, but the pathologists are comfortable differentiating between 
the true and false staining since they are familiar with this artefact.
 
Roger Heyna

 Rae Staskiewicz raest...@grics.net 5/7/2013 12:56 PM 
Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori 
antibody you are using, and whether or not you are experiencing non-specific 
staining, or staining that looks specific but with a stippled effect. 

Thanks

Rae Staskiewicz
UnityPoint Health-Methodist


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[Histonet] H pylori and Ventana instruments

[Rae Staskiewicz]

Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori 
antibody you are using, and whether or not you are experiencing non-specific 
staining, or staining that looks specific but with a stippled effect. 

Thanks

Rae Staskiewicz
UnityPoint Health-Methodist


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RE: [Histonet] H pylori and Ventana instruments

[Sebree Linda A]

Rae,

We use Ventana's HP antibody with no problems.

Linda A. Sebree

University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792

(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz
Sent: Tuesday, May 07, 2013 12:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H pylori and Ventana instruments

Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori 
antibody you are using, and whether or not you are experiencing non-specific 
staining, or staining that looks specific but with a stippled effect. 

Thanks

Rae Staskiewicz
UnityPoint Health-Methodist


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Re: [Histonet] H Pylori Stain

[Katelin Lester]

We use the HP Blue stain by Anatech.

Katelin Lester, HTL
On Jan 3, 2013 11:23 AM, Tasha Fraser tfra...@olympicmedical.org wrote:

 Hi everyone!  Does anyone have a good h-pylori stain?  We currently use
 the Steiner and Steiner Stain.  We've done some testing with the Alcian
 Yellow-Toluidine Blue Method, but not getting the same results as the
 book shows.  Does anyone have any suggestions please.



 Tasha Fraser , HT (ASCP)

 Olympic Medical Center

 Port Angeles, WA




 -
 Confidentiality Notice:  This e-mail message, including any attachments,
 is for the sole use of the intended individual(s) named above and may
 contain confidential, privileged, and/or protected information.  Any
 unauthorized review, use, disclosure, copying, or distribution of its
 contents is prohibited.  If you are not the intended recipient, you have
 received this email in error.  If so, please notify the sender immediately
 by reply email and delete/destroy the original and all copies of this
 communication.  Also know that Internet e-mail is not secure.  In choosing
 to communicate with Olympic Medical Center by email you will assume these
 confidentiality risks.   Internet messages may become corrupted,
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[Histonet] H Pylori Stain

[Tasha Fraser]

Hi everyone!  Does anyone have a good h-pylori stain?  We currently use
the Steiner and Steiner Stain.  We've done some testing with the Alcian
Yellow-Toluidine Blue Method, but not getting the same results as the
book shows.  Does anyone have any suggestions please.

 

Tasha Fraser , HT (ASCP)

Olympic Medical Center

Port Angeles, WA

 


-
Confidentiality Notice:  This e-mail message, including any attachments, is for 
the sole use of the intended individual(s) named above and may contain 
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prohibited.  If you are not the intended recipient, you have received this 
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RE: [Histonet] H Pylori Stain

[joelle weaver]

If you want a special stain- How about the good 'ole Giemsa with Jenner's? 
Works fine, is cheap and quick. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: tfra...@olympicmedical.org
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 3 Jan 2013 11:22:46 -0800
 Subject: [Histonet] H Pylori Stain
 
 Hi everyone!  Does anyone have a good h-pylori stain?  We currently use
 the Steiner and Steiner Stain.  We've done some testing with the Alcian
 Yellow-Toluidine Blue Method, but not getting the same results as the
 book shows.  Does anyone have any suggestions please.
 
  
 
 Tasha Fraser , HT (ASCP)
 
 Olympic Medical Center
 
 Port Angeles, WA
 
  
 
 
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 Confidentiality Notice:  This e-mail message, including any attachments, is 
 for the sole use of the intended individual(s) named above and may contain 
 confidential, privileged, and/or protected information.  Any unauthorized 
 review, use, disclosure, copying, or distribution of its contents is 
 prohibited.  If you are not the intended recipient, you have received this 
 email in error.  If so, please notify the sender immediately by reply email 
 and delete/destroy the original and all copies of this communication.  Also 
 know that Internet e-mail is not secure.  In choosing to communicate with 
 Olympic Medical Center by email you will assume these confidentiality risks.  
  Internet messages may become corrupted, incomplete, or may incorrectly 
 identify the sender.
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Re: [Histonet] H Pylori Stain

[Rene J Buesa]

You can find my procedure at http://www.histosearch.com/rene.html
René J.

From: Tasha Fraser tfra...@olympicmedical.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Thursday, January 3, 2013 2:22 PM
Subject: [Histonet] H Pylori Stain

Hi everyone!  Does anyone have a good h-pylori stain?  We currently use
the Steiner and Steiner Stain.  We've done some testing with the Alcian
Yellow-Toluidine Blue Method, but not getting the same results as the
book shows.  Does anyone have any suggestions please.



Tasha Fraser , HT (ASCP)

Olympic Medical Center

Port Angeles, WA




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Confidentiality Notice:  This e-mail message, including any attachments, is for 
the sole use of the intended individual(s) named above and may contain 
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Medical Center by email you will assume these confidentiality risks.  Internet 
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[Histonet] H. pylori

[Rathborne, Toni]

Happy Valentines Day!

Our lab is looking into ordering H. pylori now and I was wondering what clone, 
or whose antibody everyone is using.
As always, thanks for your responses. I know I can always count on this group 
to offer opinions and suggestions :)

Toni




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and are intended only for the addressee.  The information contained in this
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printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
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Re: [Histonet] H. pylori

[Richard Cartun]

Keep in mind that many of the commercially-available monoclonal antibodies to 
H. pylori do not label Helicobacter helilmannii, often seen in children, 
especially those with exposure to animals.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Rathborne, Toni trathbo...@somerset-healthcare.com 2/14/2012 10:21 AM 
 
Happy Valentines Day!

Our lab is looking into ordering H. pylori now and I was wondering what clone, 
or whose antibody everyone is using.
As always, thanks for your responses. I know I can always count on this group 
to offer opinions and suggestions :)

Toni




CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
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[Histonet] H Pylori Control

[Rae Staskiewicz]

Good Afternoon everyone,

 

My institution is about to start running H Pylori for another lab. They
microwave process their tissue. Would there be anyone out there who
microwave processes and would have one or two blocks of H Pylori positive
control tissue they would  be willing to let us have?

 

Thanks in advance,

 

Rae Ann Staskiewicz

Methodist Medical Center of Illinois

Peoria, IL

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[Histonet] H. Pylori - IHC

[Beth.Fye]

Does anyone have recommendations for a good vendor for H.Pylori  for IHC?  We 
have tried many, but our pathologists complain that they are not specific for 
H.Pylori and that all gram negative bacteria are staining.  Currently we are 
not running this but our pathologists are interested in it.

Thanks!

Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638
mailto:beth@hcahealthcare.com




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Re: [Histonet] H. Pylori - IHC

[Drew Meyer]

We use the Rabbit Polyclonal H. pylori from Cell Marque on the Ventana
Ultra's... We're a natinowide reference laboratory and our clients are all
very happy with the specificty of the staining.  Let me know if there's any
more information you need.

Drew
On Wed, Nov 16, 2011 at 17:15, beth@hcahealthcare.com wrote:

 Does anyone have recommendations for a good vendor for H.Pylori  for IHC?
  We have tried many, but our pathologists complain that they are not
 specific for H.Pylori and that all gram negative bacteria are staining.
  Currently we are not running this but our pathologists are interested in
 it.

 Thanks!

 Beth A. Fye, CT (ASCP)
 Pathology Technical  Manager
 HCA Richmond Hospital Laboratories
 office:  (804)228-6564
 fax: (804)323-8638
 mailto:beth@hcahealthcare.com




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RE: [Histonet] H. Pylori - IHC

[Sebree Linda A]

We use Ventana's, manufactured by Cell Marque.  Most, if not all, of out
pathologists have opted for this instead of histochemical staining for
HP. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
beth@hcahealthcare.com
Sent: Wednesday, November 16, 2011 4:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H. Pylori - IHC

Does anyone have recommendations for a good vendor for H.Pylori  for
IHC?  We have tried many, but our pathologists complain that they are
not specific for H.Pylori and that all gram negative bacteria are
staining.  Currently we are not running this but our pathologists are
interested in it.

Thanks!

Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638
mailto:beth@hcahealthcare.com




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[Histonet] H. PYLORI STAIN

[Rathborne, Toni]

Hi,
I was wondering if anyone has used the Poly Scientific Rapid Staining Method 
for H. Pylori in Gastric Biopsies staining kit.  Is it easy to use? Is the 
stain crisp and easy to read? Any feedback would be appreciated.

Toni


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and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
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exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
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Re: [Histonet] H pylori control on ventana immuno stainer

[Mark Tarango]

Hi Gloria,

Are you still looking for some good HP controls?  I could send you a block
of one of our controls if you're intersted.  I have blocks from
several different specimens.  Just let me know your address and fedex
number.

Thanks,

Mark

On Sun, Nov 21, 2010 at 12:30 PM, Gloria Cole gloria.c...@usa.net wrote:

 Hi,

 I am setting up a new lab and I am using the Ventana immuno stainer, does
 anyone know of any good HP controls I can purchase for now that will work
 well with the Ventana?

 Thanks,

 Gloria
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[Histonet] H pylori control on ventana immuno stainer

[Gloria Cole]

Hi,

I am setting up a new lab and I am using the Ventana immuno stainer, does 
anyone know of any good HP controls I can purchase for now that will work well 
with the Ventana?

Thanks,

Gloria
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[Histonet] H. pylori RTU antibody

[Drew Meyer]

I was just wondering if a lot of people use the RTU antibodies available out
there for H. pylori.  Currently, we're testing the RTU from Dako and we're
having issues with a lot of background staining.  After tweaking the
protocol a lot, I've managed to get a lot of it removed, but there are still
some issues.  I was wondering if most people are making their own dilutions
to get a better stain or if the RTUs are commonplace.  I appreciate the
feedback!

Thanks,
Drew
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RE: [Histonet] H. pylori RTU antibody

[McMahon, Loralee A]

Use the RTU from Biocare.  It is clean and easy.  You can use it with the Dako 
Flex Kit.  

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer 
[41dm...@gmail.com]
Sent: Wednesday, June 30, 2010 10:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H. pylori RTU antibody

I was just wondering if a lot of people use the RTU antibodies available out
there for H. pylori.  Currently, we're testing the RTU from Dako and we're
having issues with a lot of background staining.  After tweaking the
protocol a lot, I've managed to get a lot of it removed, but there are still
some issues.  I was wondering if most people are making their own dilutions
to get a better stain or if the RTUs are commonplace.  I appreciate the
feedback!

Thanks,
Drew
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Re: [Histonet] H. pylori RTU antibody

[Mark Tarango]

Biocare has a rabbit polyclonal anti-HP ab and a mouse monoclonal.  Which
one do you use?

Mark
On Wed, Jun 30, 2010 at 8:05 AM, McMahon, Loralee A 
loralee_mcma...@urmc.rochester.edu wrote:

 Use the RTU from Biocare.  It is clean and easy.  You can use it with the
 Dako Flex Kit.

 Loralee McMahon, HTL (ASCP)
 Immunohistochemistry Supervisor
 Strong Memorial Hospital
 Department of Surgical Pathology
 (585) 275-7210
 
 From: histonet-boun...@lists.utsouthwestern.edu [
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [
 41dm...@gmail.com]
 Sent: Wednesday, June 30, 2010 10:41 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] H. pylori RTU antibody

 I was just wondering if a lot of people use the RTU antibodies available
 out
 there for H. pylori.  Currently, we're testing the RTU from Dako and we're
 having issues with a lot of background staining.  After tweaking the
 protocol a lot, I've managed to get a lot of it removed, but there are
 still
 some issues.  I was wondering if most people are making their own dilutions
 to get a better stain or if the RTUs are commonplace.  I appreciate the
 feedback!

 Thanks,
 Drew
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[Histonet] H pylori by IHC

[Kelley Durden]

Does anyone have a recommendation for learning IHC for H pylori?
 
Nice product?
Nice protocol?
Nice learning tool?



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RE: [Histonet] H pylori by IHC

[Sebree Linda A]

Check the archives, I recently responded to Histonet on this very topic.

Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelley Durden
Sent: Friday, March 19, 2010 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H pylori by IHC


Does anyone have a recommendation for learning IHC for H pylori?
 
Nice product?
Nice protocol?
Nice learning tool?


  
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[Histonet] H. pylori on Rhesus macaque tissue

[Zerfas, Patricia (NIH/OD/ORS) [E]]

Dear List servers,
Did anyone try this antibody on Rhesus macaque tissue?  Do you 
have a protocol?  How were the results?

Thanks,

Patricia Zerfas
National Institutes of Health
Building 28A, Room 112
28 Library Drive
Bethesda, MD  20892
ph:   (301) 496-4464
fax:  (301) 402-1068


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[Histonet] H. pylori on Rhesus macaque tissue

[Amos Brooks]

Hi,
 I am sure this will work. Granted I haven't tried it... The antibody is
directed against the bug, the H. pylori bacteria, not against a product of
the human it exists in. So it shouldn't matter where the bug is, it should
have the epitope the antibody binds with. I do Hepatitis B on non human
tissue all the time and it works fine.

Good luck,
(and let me know for sure how it goes)
Amos Brooks



Message: 9
Date: Thu, 11 Mar 2010 11:25:16 -0500
From: Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov
Subject: [Histonet] H. pylori  on Rhesus macaque tissue
To: 'histonet@lists.utsouthwestern.edu'
   histonet@lists.utsouthwestern.edu
Message-ID:
   a7dde3764cfed64984b33e0373eca56b025336b...@nihmlbx09.nih.gov
Content-Type: text/plain; charset=us-ascii

Dear List servers,
   Did anyone try this antibody on Rhesus macaque tissue?  Do
you have a protocol?  How were the results?

Thanks,

Patricia Zerfas
National Institutes of Health
Building 28A, Room 112
28 Library Drive
Bethesda, MD  20892
ph:   (301) 496-4464
fax:  (301) 402-1068
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[Histonet] H pylori immuno kit

[Rutledge, Nancy]

Looking for a company that sells simple, all inclusive H pylori
immunoperoxidase kit to be done manually.

 

Please reply directly to pmea...@capecodhealth.org

 

Vendors welcome.

 

Thank you.

 

Nanacy Rutledge

Falmouth Hospital

Falmouth,

MA  02540

 

 

 

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