RE: [Histonet] HE stain uneven
I had this problem awhile back. Did you change your solvent for deparaffinization? I changed from xylene to another solvent and realized I had to extend the time my slides were in it. Betsy Molinari HT(ASCP) Texas Heart Institue Cardiovascular Pathology 1101 Bates Street Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stain uneven Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE stain uneven
Everyone thank you all so much for your helpful responses, it was my oven I called the company they fixed it everything is back to normal. Thank goodness and thanks again great job virtual team Lol Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE stain uneven
Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stain uneven
Have you checked the temperature of the heater used to dry you slides? We ran into this problem a couple of years ago and the temp of the oven was too high. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 1:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stain uneven Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stain uneven
Check your dewaxing → hydrating protocol and change reagents frequently. René J. --- On Fri, 2/11/11, Madeline Gi madelin...@yahoo.com wrote: From: Madeline Gi madelin...@yahoo.com Subject: [Histonet] HE stain uneven To: Histonet@lists.utsouthwestern.edu Date: Friday, February 11, 2011, 1:44 PM Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
