[Histonet] HS IHC project
Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an HE stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HS IHC project
T cell and B cell markers work well with human spleen. From: Patsy Ruegg pru...@ihctech.net To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu Date: 07/21/2014 11:08 AM Subject:[Histonet] HS IHC project Sent by:histonet-boun...@lists.utsouthwestern.edu Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an HE stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HS IHC project
I am so jealous of these kids. I didn't get near a microscope until college, let alone a microtome. I hope they have fun! Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Mon, Jul 21, 2014 at 2:19 PM, Jennifer MacDonald jmacdon...@mtsac.edu wrote: T cell and B cell markers work well with human spleen. From: Patsy Ruegg pru...@ihctech.net To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu Date: 07/21/2014 11:08 AM Subject:[Histonet] HS IHC project Sent by:histonet-boun...@lists.utsouthwestern.edu Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an HE stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
