Thanks, Montina
The sections are 40um and I had no trouble with all my other brains
(sectioned myself). I had help with these last two and they are all curled.
I think they just got sectioned too fast or something. I'll try the shaker
and see if it helps.
Thanks!
-Teresa
On Wed, Sep 15, 2010 at 8:47 PM, Montina Van Meter
montina.vanme...@pbrc.edu wrote:
Teresa,
I would put the sections through several 5-10 min. washes on a shaker
table. It is very important to make sure you have all of the cryoprotectant
rinsed out of the tissue or it will inhibit IHC staining. How thick are the
sections? Were they cut on a cryostat or freezing microtome? I routinely
cut rat brain at 40um and don't have any curling issues. That sometimes
occurs when the knife has come through the section of brain too rapidly. A
slow and steady motion is needed when cutting frozens.
Good luck!
Tina Van Meter
Sent from my iPhone
On Sep 15, 2010, at 9:37 PM, Teresa Iglesias tligles...@ucdavis.edu
wrote:
--
__
Teresa Iglesias
Graduate Group in Animal Behavior
Department of Evolution and Ecology
University of California-Davis
One Shields Avenue
2320 Storer Hall
Davis, CA 95616
Office: 530-754-7837
Fax: 530-752-1449
tligles...@ucdavis.edu
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