[Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Nope, sorry. All your fat is dissolved. Sent from my iPhone On Nov 13, 2012, at 8:52 AM, z o n k e d zon...@gmail.com wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating won't help. You can't replace the fat. Rena Fail On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d zon...@gmail.com wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d zon...@gmail.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 11/13/2012 08:53 AM Subject:[Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by:histonet-boun...@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
You really screwed it up! When you placed both pieces of liver in the processor both were subjected to the effect of ethanol and probably xylene and both reagents extracted the liver fat and no matter what you try to do now, there will be not enough fat in the pieces as to even try the ORO stain. Try to get another piece. Anything you will try will not render good cryosections and no fat staining. René J. From: z o n k e d zon...@gmail.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, November 13, 2012 11:52 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
