Re: [Histonet] I have a couple of quick questions
Yes. to remove mounting media you need to leave them in the Xylene after the coverslip is removed. If the mounting media is not totally removed your stain, whatever one you are doing, will not give you the results you need. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Lewis, Patrick patrick.le...@se attlechildrens.or To gHistonet@lists.utsouthwestern.edu Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] I have a couple of quick questions 10/14/2010 07:11 PM If I want to remove a cover slip that is coversliped with permount. Do I need to use xylene to get rid of the semi hardened permount gunk? Also, If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] I have a couple of quick questions
Yes, xylene is the way to go. No, 37ºC will not hurt the sections no matter for how long they are at that temp. René J. --- On Thu, 10/14/10, Lewis, Patrick patrick.le...@seattlechildrens.org wrote: From: Lewis, Patrick patrick.le...@seattlechildrens.org Subject: [Histonet] I have a couple of quick questions To: Histonet@lists.utsouthwestern.edu Date: Thursday, October 14, 2010, 7:11 PM If I want to remove a cover slip that is coversliped with permount. Do I need to use xylene to get rid of the semi hardened permount gunk? Also, If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I have a couple of quick questions
If I want to remove a cover slip that is coversliped with permount. Do I need to use xylene to get rid of the semi hardened permount gunk? Also, If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] I have a couple of quick questions
Yes, soak slides in xylene to remove coverslip. A few days in 37 degree oven won't hurt Roxanne Sent from my Verizon Wireless Phone - Reply message - From: Lewis, Patrick patrick.le...@seattlechildrens.org Date: Thu, Oct 14, 2010 7:11 pm Subject: [Histonet] I have a couple of quick questions To: Histonet@lists.utsouthwestern.edu If I want to remove a cover slip that is coversliped with permount. Do I need to use xylene to get rid of the semi hardened permount gunk? Also, If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
