[Histonet] Leica Bond Max - non OEM products?
Hello, I was referred to join this listserv due to a question we have about our Leica Bond Max. I work in a medical diagnostic laboratory. Does anyone know where we can get good quality but cheaper reagents to use on the Leica Bond Max, perhaps from a third party? The prices from Leica directly are somewhat pricey and the person who referred me said she believes that many labs use "aftermarket" supplies. I'm talking about epitope retrieval and wash solution, etc, not antibodies. Thanks in advance! Sincerely, Stephen W. Chang, MD, MBA United Medical Laboratories 1980 Gallows Rd, Ste 300 Tysons, VA 22182 703-356-4422 (phone) 703-356-2460 (fax) www.UnitedMedicalLab.com This message contains confidential information and is intended only for the individual(s) named. If you are not the named addressee you should not disseminate, distribute, or copy this email. You cannot use or forward any attachments in the email. If you have received this message in error, please notify the sender immediately and then delete this email (including any attachments) from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica bond Max
Hi all, Is there anyone interesting to the Leica bond max IHC stainer? Thanks, Kai Hong Research histotechnologist kh...@waxitinc.com 202 - 2386 East Mall Vancouver, B.C. V6T 1Z3 Canada Office: 604-822-1595 Lab: 604-822-2589 Fax: 604-822-0660 www.waxitinc.com -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Monday, February 25, 2019 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Muscle Enzyme Histochemistry (MONICA D. LOCKHART) -- Message: 1 Date: Mon, 25 Feb 2019 17:33:33 + From: "MONICA D. LOCKHART" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Muscle Enzyme Histochemistry Message-ID: <5ab0d33096f44da1bd81ad68d5be3...@luhs.org> Content-Type: text/plain; charset="us-ascii" Hello Histology World!!! Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. Thanks for your help. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockh...@luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 183, Issue 20 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond Max artifact
Hi Greg, It has been a problem for months across hundreds of kits. We added some washes and more dips in the DI water after the run to the protocol. It helps a little bit. I am going to try a more things in the next few weeks and I will keep the community updated. -Original Message- From: Greg Dobbin Sent: Thursday, October 11, 2018 11:04 AM To: linda.marg...@cookchildrens.org Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Bond Max artifact Hi Linda, Is this a problem that has just started or has the red kit always had this artefact? - If it just started, perhaps it is a problem with a particular kit lot number (or numbers). If so, get Leica Tech service involved to see if anyone else has reported a problem. - If it has been an ongoing issue for the red kit then perhaps you need to modify the Red Kit protocol by adding additional (and/or longer) wash steps. Here again Tech Service can help you with this. - The only other thing I can think to ask is: Do you have anyone new operating the instrument? Could a new person be putting undiluted Bond Wash in the bulk container? Let us know what you find out. Good luck. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond Max artifact
Hi Linda, Is this a problem that has just started or has the red kit always had this artefact? - If it just started, perhaps it is a problem with a particular kit lot number (or numbers). If so, get Leica Tech service involved to see if anyone else has reported a problem. - If it has been an ongoing issue for the red kit then perhaps you need to modify the Red Kit protocol by adding additional (and/or longer) wash steps. Here again Tech Service can help you with this. - The only other thing I can think to ask is: Do you have anyone new operating the instrument? Could a new person be putting undiluted Bond Wash in the bulk container? Let us know what you find out. Good luck. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond Max artifact
Hello Dr. Rosen and Histonetters, We have used a Leica Bond RX for over 2 years now, cleaning and caring for it just as you do. My work is mostly animal which is why we mainly choose the red chromogen alkaline phosphatase detection kit. We see the same artifact sporadically. Sometimes we will see it for several days and then maybe a week goes by and we don't see any of it. It may only be a small amount and I have seen it basically all over the slide. I know it is not related to our tissues or slides. Our Leica rep came out and increased washes but it really doesn't help. If anyone has a solution to the problem, I would also be interested. Best to all, Cathy Cathy M. Mathis Laboratory Technician IV Department of Pathology Section on Comparative Medicine - Clarkson Campus Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.1538 \ f 336.716.1515 cmmat...@wakehealth.eduwakehealth.edu -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, October 10, 2018 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 179, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Leica Bond Max artifact (Linda Margraf) 2. Re: FW: Leica Bond Max artifact (Rene J Buesa) 3. H for H. pylori? (P Sicurello) 4. Re: H for H. pylori? (Rene J Buesa) 5. Re: H for H. pylori? (Tony Henwood (SCHN)) 6. Job Opening in Massachusetts: IHC Lab Supervisor (Erin Sarricks) 7. Low Oxygen Sensor/Alarms (Cooper, Brian) -- Message: 1 Date: Tue, 9 Oct 2018 17:06:27 + From: Linda Margraf To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] FW: Leica Bond Max artifact Message-ID: Content-Type: text/plain; charset="utf-8" Here is a message I am posting for Dr. Rosen: Hello, I am a longtime admirer of this list, and I am sure someone on here can help us out We have an artifact on our immunohistochemistry (IHC) slides. I will attempt to attach pictures using the upload tool. In case that doesn?t work, I am attaching a google drive link to some jpgs. The artifact is usually golden brown to red/pink in color, is clustered, appears both on top of the tissue and around it. If we do an aggressive rundown, it appears less red/brown and bluer. We are using a Leica Bond MAX IHC system which is about a year old. We usually do two runs a day and 95% of the time we only use a red detection kit. We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H slides are fine. We started having this artifact after a month or two of production. 85% of the time we have some sort of artifact. Sometimes its perfect, sometimes it makes the tissue unreadable. We haven?t figured out any precipitating factors. We have tried a number of things, but we still can?t get control of this. Here is a brief overview of our protocols: - Processor: Leica ASP300s tissue processor. -Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) melting point 57c ? we tried switching, and it didn?t help. - We only use distilled water in our baths - Mercedes Starfrost charged slides - Oven for 30 min at 62c - We always use a cold red detection kit -Sometimes our runs are delayed but not more than 4 hours - Rundown is done in our stainer (standardized), and slides are coverslipped with tape. - The bulk fluid containers in the bond are cleaned weekly and refrigerated between runs. - The probe is cleaned every 300 slides. - Mixing wells are cleaned out every two weeks and replaced often. -Covertiles are well tended to. We have tried to correct this problem, but we can't seem to fix it. Any ideas?
[Histonet] Leica Bond Max
Good Morning, I have a question about the Leica Bond Max reagents. Has anyone ever heard of purchasing the reagents from a 3rd party rather than from Leica ? If so, what is the company's contact info. Thanks for your help. Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Bond Max - any opinions?
I would really suggest you look closely at the Intellipath from BioCare. They are a truly open system. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Tuesday, March 31, 2009 8:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Max - any opinions? Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Bond Max - any opinions?
I agree with Linda. I researched 5 different automated IHC stainers to be used for toxicology research applications, and found the intelliPATH to be the best solution for our needs. Biocare, as well, has a vast array of anti rat and mouse primary antibodies and detection systems. Additionally, the cost for the intelliPATH is quite reasonable, compared to the other platforms. Jackie O' Blazek, Linda lbla...@digestivespecialists.com Sent by: histonet-boun...@lists.utsouthwestern.edu 04/01/2009 06:22 AM To 'Michelle MacVeigh-Aloni' macve...@usc.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Leica Bond Max - any opinions? I would really suggest you look closely at the Intellipath from BioCare. They are a truly open system. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Tuesday, March 31, 2009 8:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Max - any opinions? Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond Max - any opinions?
Michelle, We also only work with animal tissue. Have you considered researching Biocare's Intellipath immunostainer? We also love their products and this is an open system. I am not the one who made the purchase decision or used both of the stainers, but, although the Leica immunostainer was also well liked, for our purposes (animal tissue), the Intellipath was chosen. Dolroes The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond Max - any opinions?
Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
