[Histonet] Light green for PAS

[Cheryl Crowder]

Michelle -


I agree that the light green is dead and you should make some new or buy a 
commercially made solution.  However, if you want to try a different 
counterstain try a light hematoylin (usually used for glycogen) or metanil 
yellow.  Either will give good contrast to the pink of the PAS.



Cheryl Crowder
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RE: [Histonet] Light Green Counterstain

[Hannen, Valerie]

 I do use a Fast Green Substitute for our Light Green counterstain.

Valerie Hannen

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lau...@blufrogpath.com
Sent: Friday, July 06, 2012 1:07 PM
To: Histonet post
Subject: [Histonet] Light Green Counterstain

Can I use fast green counterstain in place of light green for GMS and fungal 
PAS?  What is the difference between the two counterstains?

Laurie Colbert


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[Histonet] Light Green

[Breeden, Sara]

Why does my light green counter stain just stain the edges of the
sections???  

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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