RE: [Histonet] Luciferase IHC
Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Perfect!! And I was afraid Halloween was going to sneak by unnoticed! Which vendor sells holy water, 'cause I'm gonna need some... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Haven't seen it in their catalog, but you could try Erie Scientific ; ) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, November 02, 2011 9:23 AM To: O'Donnell, Bill; Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Perfect!! And I was afraid Halloween was going to sneak by unnoticed! Which vendor sells holy water, 'cause I'm gonna need some... ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
OOOH!!! Why didn't I think of that! Good goin'! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
At times like these, I wonder where Joe Nocito is... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Yeah! We've really missed JTT! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, November 02, 2011 10:04 AM To: Beckham, Sharon; O'Donnell, Bill; Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC At times like these, I wonder where Joe Nocito is... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Luciferase IHC!
Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: Beckham, Sharon s...@stowers.org Subject: RE: [Histonet] Luciferase IHC To: 'O'Donnell, Bill' billodonn...@catholichealth.net, Kim Donadio one_angel_sec...@yahoo.com, Randolph-Habecker, Julie jhabe...@fhcrc.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf98aee2...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=iso-8859-1 You know sometimes I wish these emails had a LIKE button on them. I found this quite funny and wanted to LIKE it. Too much facebook for me! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Luciferase IHC/Holy water
You can make your own holy water. You put regular water on a stove and boil the hell out of it! Mike Titford USA - Pathology Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
And it's not even Friday! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio Sent: Tue 11/1/2011 7:00 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC/Holy water
Very similar to how I cook kidneys. (ever notice why there are no great Irish restaurants or cookbooks in the U.S.? William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Wednesday, November 02, 2011 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC/Holy water You can make your own holy water. You put regular water on a stove and boil the hell out of it! Mike Titford USA - Pathology Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Luciferase IHC
Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Luciferase IHC
You have to soak it in holy water, then all H311 will break out Sorry, I couldnt resist. From: Randolph-Habecker, Julie jhabe...@fhcrc.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
