Re: [Histonet] Masson's on Frozens.

[Bob Richmond via Histonet]

Bernice Frederick HTL (ASCP) at Northwestern in Chicago USA asks:

>>When we stain and H&E on frozen [sections] we fix in 95% prior to
staining. As I never in 30 years have done a Masson's Trichrome on a
frozen section I ask: do I need to fix and then mordant as usual or go
straight to the  Bouin's and continue as normal? Do I need to mordant at
all? Tissue is rabbit liver.<<

Are you communicating with your pathologist? What are you trying to do?

The one-step trichrome techniques associated with the hallowed name of
George Gomori (particularly the Engel-Cunningham variant) are probably
preferable for frozen sections - they certainly are for skeletal muscle -
where I have a lot of experience, though many years ago. But find out
what's wanted.

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] Masson's on Frozens.

[Bernice Frederick via Histonet]

Question:
When we stain and H&E on frozen we fix in 95% prior to staining. As I never in 
30 years have done a Masson's Trichrome on a  frozen section I ask : do I need 
to fix and then mordant as usual or go straight to the  Bouin's and continue as 
normal? Do I need to mordant at all? Tissue is rabbit liver.
BErnice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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