Re: [Histonet] Metals
Thanks much. I have already printed Mallory & Parker's paper and I do have Frieda's book and will probably have to search for the others. My team deals with infectious diseases so this is a big learning curve for me. From: John Kiernan Sent: Wednesday, October 2, 2019 1:01 AM To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) ; Tony Henwood (SCHN) Cc: Histonet Subject: Re: Metals Timm's sulphide-silver method is very sensitive, and modifications (mostly by Danscher) even more so. Sulphide-silver methods detect only those metals that have insoluble sulphides (copper and zinc but not aluminium, in Jeanine's list). It is necessary to fix in a special solution containing hydrogen sulphide - stink and also serious safety precautions! Tony's mention of Mallory & Parker's fresh hematoxylin stain prompted me to look it up. The 1939 paper is a free PDF download (Google Scholar: Mallory Parker Hematoxylin Stain Metals). Mallory FB, Parker F (1939) Fixing and staining methods for lead and copper in tissues. Am. J. Pathol. 15: 517-522 and Plates 83-85. The authors noted the importance of fixation (neutral formalin was OK for copper but no good for lead, which needed 95% or 100% alcohol). Like F.B. Mallory's other papers about staining methods, it's rather vague on technical details and has no references. The late Ralph D. Lillie reported more thorough investigations of staining for metals with haematoxylin in his classic book Histopathologic Technic and Practical Histochemistry (4th and last edn 1976, ISBN 0070378622), giving colours of the complexes with 30 metals introduced into tissues. I have tried Lillie's method on paraffin sections of rat tissues containing a few of these, and it works. ISBN 9781907904325 (p.333-334) may be more accessible than Lillie's book, which has become an expensive classic. For the more specific stains mentioned in Tony's message you need to do some critical reading. The best place to start may be Frieda Carson's Histotechnology textbook. ISBN 978-0891896401. Enough about metals for now! John Kiernan London, Canada = = = From: Tony Henwood (SCHN) via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: 30 September 2019 07:22 To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) mailto:j...@cdc.gov>> Cc: Histonet mailto:histonet@lists.utsouthwestern.edu>> Subject: Re: [Histonet] Metals Two good screening stains are Mallory and Parker's Fresh Hematoxylin Stain for Metals and Timm's Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue IronBlue-black LeadBlue ZincBlue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton's Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: Monday, 30 September 2019 20:49 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not t
Re: [Histonet] Metals
☺ Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | Principal Scientist; Adjunct Fellow, School of Medicine, University of Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of Science, University of Technology Sydney | Histopathology t: (02) 9845 3306 | e: tony.henw...@health.nsw.gov.au<mailto:tony.henw...@health.nsw.gov.au> | w: www.schn.health.nsw.gov.au<http://www.schn.health.nsw.gov.au> [http://res.schn.health.nsw.gov.au/signatures/facebook.gif]<http://res.schn.health.nsw.gov.au/signatures/chw_facebook.php> [http://res.schn.health.nsw.gov.au/signatures/twitter.gif] <http://res.schn.health.nsw.gov.au/signatures/chw_twitter.php> [http://res.schn.health.nsw.gov.au/signatures/chw.gif] Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia [http://res.schn.health.nsw.gov.au/signatures/shared_banner.jpg]<http://res.schn.health.nsw.gov.au/signatures/shared_forwarder.php> ♲ Please consider the environment before printing this email. From: John Kiernan [mailto:jkier...@uwo.ca] Sent: Wednesday, 2 October 2019 3:01 PM To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) ; Tony Henwood (SCHN) Cc: Histonet Subject: Re: Metals Timm's sulphide-silver method is very sensitive, and modifications (mostly by Danscher) even more so. Sulphide-silver methods detect only those metals that have insoluble sulphides (copper and zinc but not aluminium, in Jeanine's list). It is necessary to fix in a special solution containing hydrogen sulphide - stink and also serious safety precautions! Tony's mention of Mallory & Parker's fresh hematoxylin stain prompted me to look it up. The 1939 paper is a free PDF download (Google Scholar: Mallory Parker Hematoxylin Stain Metals). Mallory FB, Parker F (1939) Fixing and staining methods for lead and copper in tissues. Am. J. Pathol. 15: 517-522 and Plates 83-85. The authors noted the importance of fixation (neutral formalin was OK for copper but no good for lead, which needed 95% or 100% alcohol). Like F.B. Mallory's other papers about staining methods, it's rather vague on technical details and has no references. The late Ralph D. Lillie reported more thorough investigations of staining for metals with haematoxylin in his classic book Histopathologic Technic and Practical Histochemistry (4th and last edn 1976, ISBN 0070378622), giving colours of the complexes with 30 metals introduced into tissues. I have tried Lillie's method on paraffin sections of rat tissues containing a few of these, and it works. ISBN 9781907904325 (p.333-334) may be more accessible than Lillie's book, which has become an expensive classic. For the more specific stains mentioned in Tony's message you need to do some critical reading. The best place to start may be Frieda Carson's Histotechnology textbook. ISBN 978-0891896401. Enough about metals for now! John Kiernan London, Canada = = = From: Tony Henwood (SCHN) via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: 30 September 2019 07:22 To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) mailto:j...@cdc.gov>> Cc: Histonet mailto:histonet@lists.utsouthwestern.edu>> Subject: Re: [Histonet] Metals Two good screening stains are Mallory and Parker’s Fresh Hematoxylin Stain for Metals and Timm’s Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue IronBlue-black LeadBlue ZincBlue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton’s Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA _
Re: [Histonet] Metals
Timm's sulphide-silver method is very sensitive, and modifications (mostly by Danscher) even more so. Sulphide-silver methods detect only those metals that have insoluble sulphides (copper and zinc but not aluminium, in Jeanine's list). It is necessary to fix in a special solution containing hydrogen sulphide - stink and also serious safety precautions! Tony's mention of Mallory & Parker's fresh hematoxylin stain prompted me to look it up. The 1939 paper is a free PDF download (Google Scholar: Mallory Parker Hematoxylin Stain Metals). Mallory FB, Parker F (1939) Fixing and staining methods for lead and copper in tissues. Am. J. Pathol. 15: 517-522 and Plates 83-85. The authors noted the importance of fixation (neutral formalin was OK for copper but no good for lead, which needed 95% or 100% alcohol). Like F.B. Mallory's other papers about staining methods, it's rather vague on technical details and has no references. The late Ralph D. Lillie reported more thorough investigations of staining for metals with haematoxylin in his classic book Histopathologic Technic and Practical Histochemistry (4th and last edn 1976, ISBN 0070378622), giving colours of the complexes with 30 metals introduced into tissues. I have tried Lillie's method on paraffin sections of rat tissues containing a few of these, and it works. ISBN 9781907904325 (p.333-334) may be more accessible than Lillie's book, which has become an expensive classic. For the more specific stains mentioned in Tony's message you need to do some critical reading. The best place to start may be Frieda Carson's Histotechnology textbook. ISBN 978-0891896401. Enough about metals for now! John Kiernan London, Canada = = = From: Tony Henwood (SCHN) via Histonet Sent: 30 September 2019 07:22 To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) Cc: Histonet Subject: Re: [Histonet] Metals Two good screening stains are Mallory and Parker’s Fresh Hematoxylin Stain for Metals and Timm’s Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue IronBlue-black LeadBlue ZincBlue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton’s Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet Sent: Monday, 30 September 2019 20:49 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Metals
Two good screening stains are Mallory and Parker’s Fresh Hematoxylin Stain for Metals and Timm’s Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue IronBlue-black LeadBlue ZincBlue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton’s Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet Sent: Monday, 30 September 2019 20:49 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Metals
Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
