Re: [Histonet] Modified Davidson solution
I agree. Making some of these simple solutions that need to be FRESH is the better way to go. As John said, there are ma ny books with these formulas in them. I have used Davidson's for different projects and I make mine in the lab fresh each time tissue is harvested. Colleen Forster HT(ASCP)QIHC On Wed, Mar 6, 2024 at 5:26 AM Tony Henwood via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I agree. > > A great book. > > > Regards, > > > > Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) > > Principal Scientist, the Children’s Hospital at Westmead (Retired) > > Adjunct Fellow, School of Medicine, University of Western Sydney. > > > From: John Kiernan via Histonet > Sent: 06 March 2024 18:32 > To: Histonet ; Naira Margaryan < > nmargarya...@gmail.com>; Davoli, Katherine A > Subject: Re: [Histonet] Modified Davidson solution > > The Davidson/Hartmann fixative is just another alcoholic formaldehyde > mixture acidified with acetic acid. Like all others of that ilk it was > intended for making up in the lab soon before using. Storage causes slow > deterioration. The ethanol slowly gets esterified by the acetic acid, > making ethyl acetate, which has no fixative properties. This change is > evident from the fruity smell of the ester, which becomes evident after a > week or two. All this has been in the textbooks for more than 50 years. > Why doesn't every lab spend perhaps $200 on a couple of books? For > obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from > the publisher, which is $30 less than Amazon's price), but there are other > histotechnology books that are very good. A book may cost much less than a > big bottle of a deteriorating mixture of questionable value, and you and > your staff could learn a lot by reading. > John Kiernan > = = = > > From: Davoli, Katherine A via Histonet > Sent: March 5, 2024 4:40 PM > To: Histonet ; Naira Margaryan < > nmargarya...@gmail.com> > Subject: Re: [Histonet] Modified Davidson solution > > Hi Naira, I get Hartmann's from Electron Microscopy Sciences > Cat# 64133-10 (for 1L, but they have larger sizes I believe) > > > Katherine Davoli, HTL(ASCP)cm(they/them/theirs) > > Lab Manager, Tissue Culture and Histology Core Facilities > > U. Pitt Dept of Ophthalmology > > 7.373 UPMC Mercy Pavilion > > 1622 Locust St., Pittsburgh PA 15219 > > (412) 624-8508 this number cannot receive texts > > > From: Naira Margaryan via Histonet > Sent: Tuesday, March 5, 2024 1:27 PM > To: Histonet > Subject: [Histonet] Modified Davidson solution > > Hello! > > What is the best company to buy a ready to use Modified Davidson solution? > > Thanks in advance, > Naira > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From: Davoli, Katherine A via Histonet > Sent: March 5, 2024 4:40 PM > To: Histonet ; Naira Margaryan < > nmargarya...@gmail.com> > Subject: Re: [Histonet] Modified Davidson solution > > Hi Naira, I get Hartmann's from Electron Microscopy Sciences > Cat# 64133-10 (for 1L, but they have larger sizes I believe) > > > Katherine Davoli, HTL(ASCP)cm(they/them/theirs) > > Lab Manager, Tissue Culture and Histology Core Facilities > > U. Pitt Dept of Ophthalmology > > 7.373 UPMC Mercy Pavilion > > 1622 Locust St., Pittsburgh PA 15219 > > (412) 624-8508 this number cannot receive texts > > > From: Naira Margaryan via Histonet > Sent: Tuesday, March 5, 2024 1:27 PM > To: Histonet > Subject: [Histonet] Modified Davidson solution > > Hello! > > What is the best company to buy a ready to use Modified Davidson solution? > > Thanks in advance, > Naira > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ &
Re: [Histonet] Modified Davidson solution
I agree. A great book. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: John Kiernan via Histonet Sent: 06 March 2024 18:32 To: Histonet ; Naira Margaryan ; Davoli, Katherine A Subject: Re: [Histonet] Modified Davidson solution The Davidson/Hartmann fixative is just another alcoholic formaldehyde mixture acidified with acetic acid. Like all others of that ilk it was intended for making up in the lab soon before using. Storage causes slow deterioration. The ethanol slowly gets esterified by the acetic acid, making ethyl acetate, which has no fixative properties. This change is evident from the fruity smell of the ester, which becomes evident after a week or two. All this has been in the textbooks for more than 50 years. Why doesn't every lab spend perhaps $200 on a couple of books? For obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from the publisher, which is $30 less than Amazon's price), but there are other histotechnology books that are very good. A book may cost much less than a big bottle of a deteriorating mixture of questionable value, and you and your staff could learn a lot by reading. John Kiernan = = = From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm(they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm(they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Modified Davidson solution
The Davidson/Hartmann fixative is just another alcoholic formaldehyde mixture acidified with acetic acid. Like all others of that ilk it was intended for making up in the lab soon before using. Storage causes slow deterioration. The ethanol slowly gets esterified by the acetic acid, making ethyl acetate, which has no fixative properties. This change is evident from the fruity smell of the ester, which becomes evident after a week or two. All this has been in the textbooks for more than 50 years. Why doesn't every lab spend perhaps $200 on a couple of books? For obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from the publisher, which is $30 less than Amazon's price), but there are other histotechnology books that are very good. A book may cost much less than a big bottle of a deteriorating mixture of questionable value, and you and your staff could learn a lot by reading. John Kiernan = = = From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm(they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm(they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Modified Davidson solution
Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm(they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=05%7C02%7Ckatherine.davoli%40pitt.edu%7C2b8c6695cc2d4e30566908dc3d4201ed%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C638452600931337419%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C=h5lm3d9fxa3KMnkU7kfLsnULNGoYzsc3BQVtiPtVLVM%3D=0<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Modified Davidson solution
Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
