Re: [Histonet] Mounting Medium Issues
Jim, I must say that I have used Eukitt exclusively for over 13 years and the only problem I have had with Eukitt is when I do not use enough and especially when coverslipping thicker sections. It is xylenes based so maybe you are experiencing excessive evaporation during drying??? If you are diluting the Eukitt with xylenes so that it flows better (not as viscious) and reduces the chance for air bubbles, this might cause excessive shrinkage and/or the problems you are experiencing. Jack On May 14, 2010, at 3:22 PM, Herrick, James L. (Jim) herrick.ja...@mayo.edu wrote: Hello everyone, Hope you have all had a good week!! Has anyone had any negative experiences using Eukitt as your mounting media? We embed our specimens (human - iliac crest, animal - femurs, tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period of time that a fairly large number of our slides are beginning to show signs of the medium breaking up below the cover slip. It looks as if there are large air pockets left behind. We are not yet sure why this is happening. Would any of you geniuses have a resolution for us or know of a better mounting media to use with plastic embedded specimens - brightfield and fluorescence? Thanks for your expertise. It is very much appreciated. Have a great weekend!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Mounting Medium Issues
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. (Jim) Sent: Friday, May 14, 2010 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium Issues Hello everyone, Hope you have all had a good week!! Has anyone had any negative experiences using Eukitt as your mounting media? We embed our specimens (human - iliac crest, animal - femurs, tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period of time that a fairly large number of our slides are beginning to show signs of the medium breaking up below the cover slip. It looks as if there are large air pockets left behind. We are not yet sure why this is happening. Would any of you geniuses have a resolution for us or know of a better mounting media to use with plastic embedded specimens - brightfield and fluorescence? Thanks for your expertise. It is very much appreciated. Have a great weekend!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting Medium Issues
Hello everyone, Hope you have all had a good week!! Has anyone had any negative experiences using Eukitt as your mounting media? We embed our specimens (human - iliac crest, animal - femurs, tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period of time that a fairly large number of our slides are beginning to show signs of the medium breaking up below the cover slip. It looks as if there are large air pockets left behind. We are not yet sure why this is happening. Would any of you geniuses have a resolution for us or know of a better mounting media to use with plastic embedded specimens - brightfield and fluorescence? Thanks for your expertise. It is very much appreciated. Have a great weekend!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
