Re: [Histonet] Need a procedure
From: John Garratt Sent: Thursday, January 23, 2020 10:51 AM To: Terri Braud Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. John www.cpqa.ca<http://www.cpqa.ca> ‐‐‐ Original Message ‐‐‐ On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet wrote: > Hi fellow Histonetters - I'm in need of some help, please > Background - We currently use agar to capture our scant cell blocks for > processing. I am unfamiliar with the Plasma/Thrombin method of cell block > preparation and am interested in comparing it to our current method > Request - Could you please send me your procedures for this method, > specifically where you purchase your plasma and thrombin and what species are > used? > Thanks in advance. Histotechs rock! > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need a procedure
Terri, when I was in animal research we used the blood from the animal to make plasma and combined it with some thrombin from the hospital pharmacy. Of course that is tedious especially since histogel is available. I warmed the histogel by placing a tube of it in a beaker of hot water, do not mw it. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: John Garratt Sent: Thursday, January 23, 2020 10:51 AM To: Terri Braud Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. John www.cpqa.ca<http://www.cpqa.ca> ‐‐‐ Original Message ‐‐‐ On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet wrote: > Hi fellow Histonetters - I'm in need of some help, please > Background - We currently use agar to capture our scant cell blocks for > processing. I am unfamiliar with the Plasma/Thrombin method of cell block > preparation and am interested in comparing it to our current method > Request - Could you please send me your procedures for this method, > specifically where you purchase your plasma and thrombin and what species are > used? > Thanks in advance. Histotechs rock! > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need a procedure
Good points but: 1. Good QC of the technique is required. The plasma has been tested for known infectious agents and (in Australia) has been treated to remove/kill these organisms. When we received the expired plasma, usually in a bag, we immediately aliquot to smaller volumes and store frozen. They are defrosted when needed. 2. The issue with molecular testing has not been proven to be an issue (I have read the paper: Balassanian, R., Ng, D. L., & van Zante, A. (2019). Stop using expired plasma for cell blocks. Cancer cytopathology, 127(12), 737-738). If contaminating human DNA is a problem, then I prefer to scrape the material from an alcohol fixed, PAP stained slide rather than a cellblock. In fact the DNA and RNA retrieved is of better quality than from a formalin-fixed cell block anyway (also refer to: Knoepp, S. M., & Roh, M. H. (2013). Ancillary techniques on direct‐smear aspirate slides: a significant evolution for cytopathology techniques. Cancer cytopathology, 121(3), 120-128). Also, has anyone assessed the deleterious effect of heat on molecular testing? 3. It needs to be remembered that an accurate diagnosis is the aim of the biopsy. Using the plasma clot method followed by formalin fixation is the same as routine core biopsy fixation, so the expected IPX results should be the same. Molecular testing will only be requested after the correct diagnosis is made. 4. I have followed those researchers that have promoted formal-substitute fixatives as being the bee's knees and undoubtedly morphology, immunohistochemistry and molecular testing are good (sometimes better) than formalin-fixed biopsies BUT I have yet to see a follow-up study on these same tissues that were fixed 5, 10 or more years ago. Is the morphology just as good or, I fear, without the protection of formalin, just mushy! Is the IPX and nucleic acids of the same quality. The lack of these follow-up studies make me worried that maybe this may have been a mistake! 5. I am not sure what the ethical issues are. Surely we are just utilising anonymous, de-identified material that would have been disposed of? Hey, my rant for the year!! -Original Message- From: John Garratt [mailto:john.garr...@ciqc.ca] Sent: Friday, 24 January 2020 11:36 AM To: Garrey Faller Cc: Tony Henwood (SCHN) ; Muhammad Azam ; Terri Braud ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure Good point regarding the molecular and another good reason to move away from plasma. Plus taking plasma, even outdated plasma, is fraught with ethical and regularity problems which are best to avoid now that ever milliliter of blood product needs to be accounted for. www.cpqa.ca ‐‐‐ Original Message ‐‐‐ On Thursday, January 23, 2020 3:41 PM, Garrey Faller wrote: > Great helpful information. > We use plasma /thrombin. > It’s easy to use, and we have plenty of access to it from our blood bank. > > However, we are looking to switch to Gel. > > Why? > > 1. If you don’t keep an eye on your plasma, at some point you could see > fungi in your specimen with a chance you might think it’s real and not a > contaminant. That’s a problem. Don’t keep it for too long in the > refrigerator. It’s a great culture medium for fungi. > You could freeze small aliquots I guess. > > 2. Molecular testing on cell blocks: > With the increasing use of molecular testing on cell blocks, plasma > thrombin method poses a problem. The plasma introduces other peoples DNA into > the patients sample.Think about that. > > Didn’t know about the heat issue with Gel. Important to know. Thanks ! > > Garrey > > Sent from my iPhone > > > > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet > > histonet@lists.utsouthwestern.edu wrote: > > Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the > > matrix (eg agar or other commercial matrixes like Histogel). > > Adding a heated matrix to unfixed, or even formalin fixed, material can > > denature some antigens (eg CEA) resulting in a false negative IPX. > > Unfortunately the importance of heat as a pre-analytical factor in > > immunohistochemistry is often not appreciated. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > > Principal Scientist, the Children’s Hospital at Westmead Adjunct > > Fellow, School of Medicine, University of Western Sydney > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > Pathology Department > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > > Westmead NSW 2145, AUSTRALIA -Original Message- > > From: John Garratt via Histonet > > [mailto:histonet@lists.utsouthwestern.edu] &g
Re: [Histonet] Need a procedure
Tony has great point. I used to use Histogel (research for cell lines but applicable to cell block). Switched to Low-melt agarose because of the temp consideration on unfixed cells. Low-temp is a little misleading. Melts (in oven at over 60 degrees) but COOL IT and remains liquid at 37degrees (body temp) and some do not gel until 26 degrees. RNA/DNA-ase free so molecular is fine. Temp low 37 degrees (when used to mix cells) so no DNA denaturation and proteins fine. That was 10 years ago. Just checked there are all sorts of "low-melt" agarose out there. Ray, Fair Director, Eastern Washington Regional Science and Engineering Fair. > On January 23, 2020 at 2:59 PM "Tony Henwood (SCHN) via Histonet" > wrote: > > > Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the > matrix (eg agar or other commercial matrixes like Histogel). > Adding a heated matrix to unfixed, or even formalin fixed, material can > denature some antigens (eg CEA) resulting in a false negative IPX. > Unfortunately the importance of heat as a pre-analytical factor in > immunohistochemistry is often not appreciated. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children’s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -Original Message- > From: John Garratt via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Friday, 24 January 2020 8:21 AM > To: Muhammad Azam > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Need a procedure > > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf > > > The above link will help. > > > > www.cpqa.ca > > ‐‐‐ Original Message ‐‐‐ > On Thursday, January 23, 2020 12:13 PM, Muhammad Azam > wrote: > > > Anybody has validated procedure for histogel > > > > Sent from my iPhone > > > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet > > > histonet@lists.utsouthwestern.edu wrote: > > > Hi Terri, I suggest you use Histogel for block preparation. It works > > > exceptionally well, it is good for IHC and does not have the pitfalls of > > > plasma/thrombin. > > > Plasma/thrombin does work well for cell blocks but you will have to > > > consider an ethical and safe source for your plasma. > > > The instructions for using Histogel are in the package insert though I > > > have one comment. Be careful how you warm the Histogel and use a heat > > > block. Do NOT use a microwave since there is a tendency to overheat the > > > gel and you will end up with poor quality IHC. > > > John > > > www.cpqa.ca > > > ‐‐‐ Original Message ‐‐‐ > > > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet > > > > histonet@lists.utsouthwestern.edu wrote: > > > > Hi fellow Histonetters - I'm in need of some help, please > > > > Background - We currently use agar to capture our scant cell > > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method > > > > of cell block preparation and am interested in comparing it to our > > > > current method Request - Could you please send me your procedures for > > > > this method, specifically where you purchase your plasma and thrombin > > > > and what species are used? > > > > Thanks in advance. Histotechs rock! > > > > Terri L. Braud, HT(ASCP) > > > > Anatomic Pathology Supervisor > > > > Laboratory > > > > Holy Redeemer Hospital > > > > 1648 Huntingdon Pike > > > > Meadowbrook, PA 19046 > > > > ph: 215-938-3689 > > > > fax: 215-938-3874 > > > > Care, Comfort, and Heal > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > __
Re: [Histonet] Need a procedure
Good point regarding the molecular and another good reason to move away from plasma. Plus taking plasma, even outdated plasma, is fraught with ethical and regularity problems which are best to avoid now that ever milliliter of blood product needs to be accounted for. www.cpqa.ca ‐‐‐ Original Message ‐‐‐ On Thursday, January 23, 2020 3:41 PM, Garrey Faller wrote: > Great helpful information. > We use plasma /thrombin. > It’s easy to use, and we have plenty of access to it from our blood bank. > > However, we are looking to switch to Gel. > > Why? > > 1. If you don’t keep an eye on your plasma, at some point you could see > fungi in your specimen with a chance you might think it’s real and not a > contaminant. That’s a problem. Don’t keep it for too long in the > refrigerator. It’s a great culture medium for fungi. > You could freeze small aliquots I guess. > > 2. Molecular testing on cell blocks: > With the increasing use of molecular testing on cell blocks, plasma > thrombin method poses a problem. The plasma introduces other peoples DNA into > the patients sample.Think about that. > > Didn’t know about the heat issue with Gel. Important to know. Thanks ! > > Garrey > > Sent from my iPhone > > > > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet > > histonet@lists.utsouthwestern.edu wrote: > > Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the > > matrix (eg agar or other commercial matrixes like Histogel). > > Adding a heated matrix to unfixed, or even formalin fixed, material can > > denature some antigens (eg CEA) resulting in a false negative IPX. > > Unfortunately the importance of heat as a pre-analytical factor in > > immunohistochemistry is often not appreciated. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > > Principal Scientist, the Children’s Hospital at Westmead > > Adjunct Fellow, School of Medicine, University of Western Sydney > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > Pathology Department > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -Original Message- > > From: John Garratt via Histonet [mailto:histonet@lists.utsouthwestern.edu] > > Sent: Friday, 24 January 2020 8:21 AM > > To: Muhammad Azam ajj...@gmail.com > > Cc: histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Need a procedure > > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD Documents/Product Manuals > > & Specifications/Histology Equipment and Supplies/Embedding Cassettes and > > Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf > > The above link will help. > > www.cpqa.ca > > ‐‐‐ Original Message ‐‐‐ > > > > > On Thursday, January 23, 2020 12:13 PM, Muhammad Azam ajj...@gmail.com > > > wrote: > > > Anybody has validated procedure for histogel > > > Sent from my iPhone > > > > > > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet > > > > > histonet@lists.utsouthwestern.edu wrote: > > > > > Hi Terri, I suggest you use Histogel for block preparation. It works > > > > > exceptionally well, it is good for IHC and does not have the pitfalls > > > > > of plasma/thrombin. > > > > > Plasma/thrombin does work well for cell blocks but you will have to > > > > > consider an ethical and safe source for your plasma. > > > > > The instructions for using Histogel are in the package insert though > > > > > I have one comment. Be careful how you warm the Histogel and use a > > > > > heat block. Do NOT use a microwave since there is a tendency to > > > > > overheat the gel and you will end up with poor quality IHC. > > > > > John > > > > > www.cpqa.ca > > > > > ‐‐‐ Original Message ‐‐‐ > > > > > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet > > > > > histonet@lists.utsouthwestern.edu wrote: > > > > > Hi fellow Histonetters - I'm in need of some help, please > > > > > Background - We currently use agar to capture our scant cell > > > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin > > > > > method of cell block preparation and am interested in comparing it to > > > > > our current method Request - Could you please send me your procedures &
Re: [Histonet] Need a procedure
Great helpful information. We use plasma /thrombin. It’s easy to use, and we have plenty of access to it from our blood bank. However, we are looking to switch to Gel. Why? 1. If you don’t keep an eye on your plasma, at some point you could see fungi in your specimen with a chance you might think it’s real and not a contaminant. That’s a problem. Don’t keep it for too long in the refrigerator. It’s a great culture medium for fungi. You could freeze small aliquots I guess. 2. Molecular testing on cell blocks: With the increasing use of molecular testing on cell blocks, plasma thrombin method poses a problem. The plasma introduces other peoples DNA into the patients sample.Think about that. Didn’t know about the heat issue with Gel. Important to know. Thanks ! Garrey Sent from my iPhone > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet > wrote: > > Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the > matrix (eg agar or other commercial matrixes like Histogel). > Adding a heated matrix to unfixed, or even formalin fixed, material can > denature some antigens (eg CEA) resulting in a false negative IPX. > Unfortunately the importance of heat as a pre-analytical factor in > immunohistochemistry is often not appreciated. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children’s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -Original Message- > From: John Garratt via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Friday, 24 January 2020 8:21 AM > To: Muhammad Azam > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Need a procedure > > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf > > > The above link will help. > > > > www.cpqa.ca > > ‐‐‐ Original Message ‐‐‐ >> On Thursday, January 23, 2020 12:13 PM, Muhammad Azam >> wrote: >> >> Anybody has validated procedure for histogel >> >> Sent from my iPhone >> >>>> On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet >>>> histonet@lists.utsouthwestern.edu wrote: >>> Hi Terri, I suggest you use Histogel for block preparation. It works >>> exceptionally well, it is good for IHC and does not have the pitfalls of >>> plasma/thrombin. >>> Plasma/thrombin does work well for cell blocks but you will have to >>> consider an ethical and safe source for your plasma. >>> The instructions for using Histogel are in the package insert though I have >>> one comment. Be careful how you warm the Histogel and use a heat block. Do >>> NOT use a microwave since there is a tendency to overheat the gel and you >>> will end up with poor quality IHC. >>> John >>> www.cpqa.ca >>> ‐‐‐ Original Message ‐‐‐ >>> >>>> On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet >>>> histonet@lists.utsouthwestern.edu wrote: >>>> Hi fellow Histonetters - I'm in need of some help, please >>>> Background - We currently use agar to capture our scant cell >>>> blocks for processing. I am unfamiliar with the Plasma/Thrombin method of >>>> cell block preparation and am interested in comparing it to our current >>>> method Request - Could you please send me your procedures for this method, >>>> specifically where you purchase your plasma and thrombin and what species >>>> are used? >>>> Thanks in advance. Histotechs rock! >>>> Terri L. Braud, HT(ASCP) >>>> Anatomic Pathology Supervisor >>>> Laboratory >>>> Holy Redeemer Hospital >>>> 1648 Huntingdon Pike >>>> Meadowbrook, PA 19046 >>>> ph: 215-938-3689 >>>> fax: 215-938-3874 >>>> Care, Comfort, and Heal >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > &g
Re: [Histonet] Need a procedure
Hi all, Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel). Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX. Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: John Garratt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, 24 January 2020 8:21 AM To: Muhammad Azam Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf The above link will help. www.cpqa.ca ‐‐‐ Original Message ‐‐‐ On Thursday, January 23, 2020 12:13 PM, Muhammad Azam wrote: > Anybody has validated procedure for histogel > > Sent from my iPhone > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet > > histonet@lists.utsouthwestern.edu wrote: > > Hi Terri, I suggest you use Histogel for block preparation. It works > > exceptionally well, it is good for IHC and does not have the pitfalls of > > plasma/thrombin. > > Plasma/thrombin does work well for cell blocks but you will have to > > consider an ethical and safe source for your plasma. > > The instructions for using Histogel are in the package insert though I have > > one comment. Be careful how you warm the Histogel and use a heat block. Do > > NOT use a microwave since there is a tendency to overheat the gel and you > > will end up with poor quality IHC. > > John > > www.cpqa.ca > > ‐‐‐ Original Message ‐‐‐ > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet > > > histonet@lists.utsouthwestern.edu wrote: > > > Hi fellow Histonetters - I'm in need of some help, please > > > Background - We currently use agar to capture our scant cell > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method of > > > cell block preparation and am interested in comparing it to our current > > > method Request - Could you please send me your procedures for this > > > method, specifically where you purchase your plasma and thrombin and what > > > species are used? > > > Thanks in advance. Histotechs rock! > > > Terri L. Braud, HT(ASCP) > > > Anatomic Pathology Supervisor > > > Laboratory > > > Holy Redeemer Hospital > > > 1648 Huntingdon Pike > > > Meadowbrook, PA 19046 > > > ph: 215-938-3689 > > > fax: 215-938-3874 > > > Care, Comfort, and Heal > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need a procedure
Anybody has validated procedure for histogel Sent from my iPhone > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet > wrote: > > Hi Terri, I suggest you use Histogel for block preparation. It works > exceptionally well, it is good for IHC and does not have the pitfalls of > plasma/thrombin. > Plasma/thrombin does work well for cell blocks but you will have to consider > an ethical and safe source for your plasma. > The instructions for using Histogel are in the package insert though I have > one comment. Be careful how you warm the Histogel and use a heat block. Do > NOT use a microwave since there is a tendency to overheat the gel and you > will end up with poor quality IHC. > > John > > > www.cpqa.ca > > ‐‐‐ Original Message ‐‐‐ >> On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet >> wrote: >> >> Hi fellow Histonetters - I'm in need of some help, please >> Background - We currently use agar to capture our scant cell blocks for >> processing. I am unfamiliar with the Plasma/Thrombin method of cell block >> preparation and am interested in comparing it to our current method >> Request - Could you please send me your procedures for this method, >> specifically where you purchase your plasma and thrombin and what species >> are used? >> Thanks in advance. Histotechs rock! >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need a procedure
Terri, I agree with John on the use of Histogel. I use it routinely for all the cell blocks and tiny matrix samples that come down and I get excellent results. I do a lot of IHC and the results are good. Respectfully, Colleen On Thu, Jan 23, 2020 at 11:51 AM John Garratt via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Terri, I suggest you use Histogel for block preparation. It works > exceptionally well, it is good for IHC and does not have the pitfalls of > plasma/thrombin. > Plasma/thrombin does work well for cell blocks but you will have to > consider an ethical and safe source for your plasma. > The instructions for using Histogel are in the package insert though I > have one comment. Be careful how you warm the Histogel and use a heat > block. Do NOT use a microwave since there is a tendency to overheat the gel > and you will end up with poor quality IHC. > > John > > > www.cpqa.ca > > ‐‐‐ Original Message ‐‐‐ > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Hi fellow Histonetters - I'm in need of some help, please > > Background - We currently use agar to capture our scant cell blocks for > processing. I am unfamiliar with the Plasma/Thrombin method of cell block > preparation and am interested in comparing it to our current method > > Request - Could you please send me your procedures for this method, > specifically where you purchase your plasma and thrombin and what species > are used? > > Thanks in advance. Histotechs rock! > > > > Terri L. Braud, HT(ASCP) > > Anatomic Pathology Supervisor > > Laboratory > > Holy Redeemer Hospital > > 1648 Huntingdon Pike > > Meadowbrook, PA 19046 > > ph: 215-938-3689 > > fax: 215-938-3874 > > Care, Comfort, and Heal > > > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need a procedure
Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. John www.cpqa.ca ‐‐‐ Original Message ‐‐‐ On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet wrote: > Hi fellow Histonetters - I'm in need of some help, please > Background - We currently use agar to capture our scant cell blocks for > processing. I am unfamiliar with the Plasma/Thrombin method of cell block > preparation and am interested in comparing it to our current method > Request - Could you please send me your procedures for this method, > specifically where you purchase your plasma and thrombin and what species are > used? > Thanks in advance. Histotechs rock! > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need a procedure
Hi fellow Histonetters - I'm in need of some help, please Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? Thanks in advance. Histotechs rock! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
