I used to do a stain on post mortem heart slices to look for recent
infarctions. the entire tissue turned a sort of bluey colour which remained
through formain fixation and processing. the tissue however was incubated in
solution of NBT in a sodium cyanide containing buffer - not sure if that
would still be allowed these days - but if you want - i could send you the
protocol we used
best regards
On Thu, Oct 28, 2010 at 8:51 PM, Laurie Colbert
laurie.colb...@huntingtonhospital.com wrote:
I previously posted a question regarding Nitroblue Tetrazolium Chloride,
but I didn't really receive the info that I needed - so I thought I
would ask again.
I need to purchase this item for a research doc. He wants to immerse
tissue in this solution for 24 hours and then process as usual. It is
my understanding that this is some kind of dye. I see that I can order
it from Sigma in tablet form. I've seen it from other companies in a
powder form.
Has anyone ever used this reagent in the capacity that I describe? Is
it available as a ready-to-use solution/liquid? Is there a certain
strength/percentage that I should use?
Thanks,
Laurie Colbert
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Bone Research Unit
University of the Witwatersrand
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