RE: [Histonet] Osteoclast
You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it to work on formic acid decaled bone. I also believe that there is an antibody for osteoclasts, but I have not tried it. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante [lbustama...@cvm.tamu.edu] Sent: Tuesday, October 05, 2010 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osteoclast We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Osteoclast
Hi Loralee, I am having similar problems with Formic acid Decal'd sections for TRAP. After EDTA, are you doing a TRAP IHC or the enzyme assay from Sigma? As for molecular markers, Cathepsin K is pretty good for Osteoclasts but I do westerns in a research setting..has anyone had good success with any antibody for immunostaining? Best, Praveen Arany, Graduate Student, Harvard University, Cambridge MA On 10/6/2010 8:43 AM, McMahon, Loralee A wrote: You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it to work on formic acid decaled bone. I also believe that there is an antibody for osteoclasts, but I have not tried it. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante [lbustama...@cvm.tamu.edu] Sent: Tuesday, October 05, 2010 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osteoclast We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Osteoclast
We were never able to get the TRAP working with Formic Acid decal. Using a home brewed TRAP kit or the Sigma Kit. But Cell Marque has a TRAcP antibody (catalog 341M-95) that is used as a hairy cell marker. But it also stains macrophages and osteoclasts. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Praveen Arany [ar...@fas.harvard.edu] Sent: Wednesday, October 06, 2010 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Osteoclast Hi Loralee, I am having similar problems with Formic acid Decal'd sections for TRAP. After EDTA, are you doing a TRAP IHC or the enzyme assay from Sigma? As for molecular markers, Cathepsin K is pretty good for Osteoclasts but I do westerns in a research setting..has anyone had good success with any antibody for immunostaining? Best, Praveen Arany, Graduate Student, Harvard University, Cambridge MA On 10/6/2010 8:43 AM, McMahon, Loralee A wrote: You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it to work on formic acid decaled bone. I also believe that there is an antibody for osteoclasts, but I have not tried it. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante [lbustama...@cvm.tamu.edu] Sent: Tuesday, October 05, 2010 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osteoclast We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Osteoclast
We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Osteoclast
You can do TRAP ( Tartrate-resistant Acidic Phosphatase) stain for osteoclasts. On 10/5/10 4:58 PM, Lin Bustamante lbustama...@cvm.tamu.edu wrote: We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
