[Histonet] Processor Failure Troubleshooting

[jdhannasch--- via Histonet]

Hello everyone,

I’ve been fortunate enough to not deal with too many processor problems like a 
processor breaking down mid schedule or someone putting the wrong chemicals in 
wrong spot on the processor, but I want to plan for when these occurrences 
inevitably happens. Does anyone have any resources they could share about this? 
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[Histonet] Processor

[Anne Murvosh via Histonet]

I have a back-up processor we keep filled for a back-up and if we have an extra 
run. However with lower volumes lately we aren't using it. I was just wondering 
about the paraffin in particularly. Does anyone know if you are not using it 
how long it can stay there (its heated) before changing out completely? Does it 
lose its stability? Thanks Anne Murvosh (HT)
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Re: [Histonet] processor died overnight

[Tony Henwood (SCHN) via Histonet]

Yep start at last alcohol in other processor, xylene & wax as usual.
If the tissues have remained covered in alcohol, you should not have a problem

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 19 April 2017 10:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] processor died overnight

Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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Re: [Histonet] processor died overnight

[Mayer,Toysha N via Histonet]

To help this you could place the cassettes in the other processor in the last 
100% for about 5 min, just to freshen them up.  Then proceed with the remainder 
of the process as usual.  

FYI, this will be one of our discussion questions this year in our HTL program.

T
Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
Instructor/Education Coordinator
HTL Program
MD Anderson School of Health Professions
713.563.3481
tnma...@mdanderson.org





Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.






--

Message: 5
Date: Wed, 19 Apr 2017 13:41:47 + (UTC)
From: Rene J Buesa <rjbu...@yahoo.com>
To: Lauren Sweeney <lmari...@uga.edu>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] processor died overnight
Message-ID: <174147213.3806623.1492609307...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
program to continue the steps until melted paraffin.If there are delicate 
tissue perhaps they will be "over-dried" but that is easily "compensated" 
during microtomy.Ren? 

On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
<histonet@lists.utsouthwestern.edu> wrote:
 

 Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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--

Message: 6
Date: Wed, 19 Apr 2017 16:11:51 +
From: "Terri  Braud" <tbr...@holyredeemer.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] IF vs IHC another reason
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F8130EAF@HRHEX03-HOS.holyredeemer.local>

Content-Type: text/plain; charset="us-ascii"


Another reason that IHC is used instead of IF is with IHC, one preserves the 
ability to see tissue/cell morphology through Light Microscopy at the same time 
as the visual IHC label.  Morphology is difficult to see with IF, with the 
exception of the fluorescein labeled area.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 13 April 2017 11:10 PM
Hello!
I just need a help with a simple question...Is anyone can explain me what is 
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)
Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu





--

Message: 7
Date: Wed, 19 Apr 2017 09:17:24 -0700
From: Caroline Miller <mi...@3scan.com>
To: Rene J Buesa <rjbu...@yahoo.com>
Cc: Lauren Sweeney <lmari...@uga.edu>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] processor died overnight
Message-ID: <6156911d-ede8-4c77-9c6d-36ba64baf...@3scan.com>
Content-Type: text/plain;   charset=utf-8

Yes, totally +1 to Rene, they should be fine. 
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
> program to continue the steps until melted paraffin.If there are delicate 
> tissue perhaps they will be "over-dried" but that is easily "compensated" 
> during microtomy.Ren? 
> 
>On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> 
> Hello Histoworld,
> 
> I came in this morning to find that the processor died half

Re: [Histonet] processor died overnight

[McNabola, Angela via Histonet]

It has happened to us as well.  I'm not sure where you work, but our processors 
have alarms that go to security.  So I have gotten a call on off hours to come 
in and fix the problem or try to trouble shoot.  Some of the newer processors 
can be linked to your phone, etc.

-Original Message-
From: Caroline Miller via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 19, 2017 12:17 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] processor died overnight

Yes, totally +1 to Rene, they should be fine.
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
>
> In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
> program to continue the steps until melted paraffin.If there are delicate 
> tissue perhaps they will be "over-dried" but that is easily "compensated" 
> during microtomy.René
>
>On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
>
>
> Hello Histoworld,
>
> I came in this morning to find that the processor died halfway through 
> process last night. The tissues are in 100% ETOH exactly half point. We do 
> have a back- up processor. In your professional experiences, would these 
> tissues be salvageable? Could I create a new program on the backup processor 
> that finishes the process from that point and transfer the tissues over?
>
> Thanks.
>
>
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ___
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Re: [Histonet] processor died overnight

[Caroline Miller via Histonet]

Yes, totally +1 to Rene, they should be fine. 
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet 
>  wrote:
> 
> In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
> program to continue the steps until melted paraffin.If there are delicate 
> tissue perhaps they will be "over-dried" but that is easily "compensated" 
> during microtomy.René 
> 
>On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
>  wrote:
> 
> 
> Hello Histoworld,
> 
> I came in this morning to find that the processor died halfway through 
> process last night. The tissues are in 100% ETOH exactly half point. We do 
> have a back- up processor. In your professional experiences, would these 
> tissues be salvageable? Could I create a new program on the backup processor 
> that finishes the process from that point and transfer the tissues over?
> 
> Thanks.
> 
> 
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> 
> 
> 
> ___
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Re: [Histonet] processor died overnight

[Rene J Buesa via Histonet]

In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
program to continue the steps until melted paraffin.If there are delicate 
tissue perhaps they will be "over-dried" but that is easily "compensated" 
during microtomy.René 

On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
 wrote:
 

 Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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[Histonet] processor died overnight

[Lauren Sweeney via Histonet]

Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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[Histonet] Processor Troubleshooting

[Jayasri Narasimhan via Histonet]

Hi everyone,

For the past two months in the research lab I work in, we've been dealing with 
poorly processed mouse livers. The following is the protocol, which is 
optimized for mouse mammary tissue, but we've been using it for all tissues.

Fixation:
~48 hours in 10% nbf, shaking.
70% ethanol until processing; it sits in there anywhere from a couple of days 
to weeks.

Processing (Sakura Tissue Tek VIP - 5)
70% ethanol   5 min
80%30 min
95% - 1  30 min
95% - 2  40 min
100% -130 min
100% -230 min
100%-2 30 min
Xylene-1  45 min
Xylene-2  40 min
Xylene-3  30 min
Paraffin1  40 min
Paraffin2  45 min
Paraffin3  45 min

The worst livers expanded on the ice bath, and had the hepatocytes appeared to 
have perinuclear cytoplasmic clearing. We ran PAS and glycogen doesn't fully 
account for their appearance.

We've pm'ed our processor, compared runs on our processor to runs from the 
research histocore, and processed a few cassettes in surgical pathology using 
their protocol.

By far, livers processed in Surg Path were the best. Their protocol starts with 
NBF and then goes into 80% alchohol. From then, each cycle is 40-60 minutes, so 
a bit longer than ours.

Finally, the Sakura tech ran a warm water flush. The specimens run after this 
flush look similar to the ones run at the research histocore, so the processing 
problems seem to be resolved.

My main question: what could the warm flush have done to resolve our processing 
issues? Since we're storing our tissues in 70% before putting them on the 
processor, aren't we preventing salt precipitation that could affect 
processing? Does anyone know the chemistry/science behind what might have 
happened here?

Thanks,
JN
OHSU Lab

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[Histonet] Processor Validation

[Diana Martinez-Longoria via Histonet]

Hello Histonetters,



I was wondering if any of you have processor runs for 2 and 4 hour? I am trying 
to run validations for our upcoming CAP inspection. The processor that we are 
using is Tissue Tek VIP 5 Vacuum Infiltration Processor. This will greatly help 
me out. Thanks in advance!



Diana Martinez-Longoria

Bachelors of Science in Biology

 Histotechnician (ASCP)cm

El Centro Regional Medical Center

Phone: 760-339-7267

Fax: 760-339-4570

Email:dmlongo...@ecrmc.org




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Re: [Histonet] Processor

[Tina Van Meter]

​I beg to differThe VIP 5 is capable of processing 300 cassettes. On
the first page of my VIP 5, Operating Manual (p. 1.1), it says it is
capable of handling 150-300 cassettes.


Tina Van Meter
Histology Core Manager
Scripps Research Institute
Jupiter, FL

On Tue, Dec 2, 2014 at 3:22 AM, Jamal j.rowa...@alborglaboratories.com
wrote:

 Hi
 Sakura VIP 5 dose not process more than 150 cassettes per day

 I recommend Sakura VIP 6 or Leica ASP 6025


 Best Regards,


 Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
 Medical Laboratories |  Mobile +966 503629832|
 j.rowa...@alborglaboratories.com
 Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
 Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
 www.alborglaboratories.com


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tina Van Meter
 Sent: Monday, December 01, 2014 10:15 PM
 To: Abbott, Tanya
 Cc: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Processor

 Sakura VIP 5 or 6​

 On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya 
 tanyaabb...@catholichealth.net wrote:

  Looking for a tissue processor to process 200-300 cassettes per run, any
  favourites?
 
  Tanya G. Abbott RT (CSMLS)
  Manager Technologist, Histology/Cytology
  St. Joseph Medical Center
  Reading, PA 19603-0316
  ph  610-378-2635
  fax 610-898-5871
  email: tanyaabb...@catholichealth.net
 
  This email and attachments contain information that may be confidential
 or
  privileged. If you are not the intended recipient, notify the sender at
  once and delete this message completely from your information system.
  Further use, disclosure, or copying of information contained in this
 email
  is not authorized, and any such action should not be construed as a
 waiver
  of privilege or other confidentiality protections.
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[Histonet] Processor

[Abbott, Tanya]

Looking for a tissue processor to process 200-300 cassettes per run, any 
favourites?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
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Re: [Histonet] Processor

[Tina Van Meter]

Sakura VIP 5 or 6​

On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya 
tanyaabb...@catholichealth.net wrote:

 Looking for a tissue processor to process 200-300 cassettes per run, any
 favourites?

 Tanya G. Abbott RT (CSMLS)
 Manager Technologist, Histology/Cytology
 St. Joseph Medical Center
 Reading, PA 19603-0316
 ph  610-378-2635
 fax 610-898-5871
 email: tanyaabb...@catholichealth.net

 This email and attachments contain information that may be confidential or
 privileged. If you are not the intended recipient, notify the sender at
 once and delete this message completely from your information system.
 Further use, disclosure, or copying of information contained in this email
 is not authorized, and any such action should not be construed as a waiver
 of privilege or other confidentiality protections.
 ___
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Processor

[Joelle Weaver]

Agree with VIP for conventional tissue processing


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
 Date: Mon, 1 Dec 2014 14:15:23 -0500
 From: tina.vanme...@gmail.com
 To: tanyaabb...@catholichealth.net
 Subject: Re: [Histonet] Processor
 CC: histonet@lists.utsouthwestern.edu
 
 Sakura VIP 5 or 6​
 
 On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya 
 tanyaabb...@catholichealth.net wrote:
 
  Looking for a tissue processor to process 200-300 cassettes per run, any
  favourites?
 
  Tanya G. Abbott RT (CSMLS)
  Manager Technologist, Histology/Cytology
  St. Joseph Medical Center
  Reading, PA 19603-0316
  ph  610-378-2635
  fax 610-898-5871
  email: tanyaabb...@catholichealth.net
 
  This email and attachments contain information that may be confidential or
  privileged. If you are not the intended recipient, notify the sender at
  once and delete this message completely from your information system.
  Further use, disclosure, or copying of information contained in this email
  is not authorized, and any such action should not be construed as a waiver
  of privilege or other confidentiality protections.
  ___
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[Histonet] Processor Alarms

[Cristi Stephenson]

Hello Histoland,

We have an ASP300S processor that is not currently monitored by Leica.  I
was wondering if anyone had an alarm system suggestion such that, in the
event it does malfunction, it will notify a designated person.  We are a
private practice so there is no one in the building after normal working
hours.

Thanks in advance for any recommendations!

Cristi
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[Histonet] Processor problem

[Mitchell Wan (Wanpost)]

Hi Mike,
Edit the 'dummy' run and make station 3 with '0' time.
It is time based and has a safety mechanism. That's why it pumps the previous 
station. Then test again.

A couple things may be an issue and need to be tested.
1. A hole, leak in the line of station 3.
2. Volume in reservoir bottle is too high
3. Kink in tube
4. Valve 'sticking'. Do the verify 7,8 test to hear the valve click in and out.
A drop of xylene will fix this. Do this under supervision. 
5. Check valve pressure and vacuum. View from behind and see the guage.
6. Clog in line 3. Do a hot water flush. 2-3 times.

Regards
Mitchell Wan
0418 745 750

P.O. BOX 2200,
Runcorn, 
Brisbane,
QLD 4113





 Original message 
From: histonet-requ...@lists.utsouthwestern.edu 
Date:08/06/2014  3:03 AM  (GMT+10:00) 
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 127, Issue 10 

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Today's Topics:

   1. Processor problem (Mike Tighe)
   2. RE: Processor malfunction - tissues not submerged ~ 9 hours
  (Shirley A. Powell)
   3. paraffin dispenser (Davis, Cassie)
   4. Re: Processor malfunction - tissues not submerged ~ 9 hours
  (jsjurc...@comcast.net)
   5. Re: Processor problem (Rene J Buesa)
   6. RE: Processor problem (Denice Stiner)
   7. VIP3000 reagent bottle (Mitchell Wan (Wanpost))


--

Message: 1
Date: Fri, 6 Jun 2014 17:52:27 +
From: Mike Tighe mti...@trudeauinstitute.org
Subject: [Histonet] Processor problem
To: histonet@lists.utsouthwestern.edu
(histonet@lists.utsouthwestern.edu)
histonet@lists.utsouthwestern.edu
Message-ID: 1402077076740.7...@trudeauinstitute.org
Content-Type: text/plain; charset=iso-8859-1

I am having a problem with our tissue processor (Tissue Tek 3000). During 
processing the first reagent (NBF) gets pumped into the retort without any 
problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 
70%) however, gets about half way through pumping into retort and stops. After 
that it reverses and pumps out reagent three, then pumps in half of reagent 2. 
Pumps out the half of reagent 2 and pumps in half of reagent 1. This is 
followed by a low fluid warning.



I have removed and reinserted the reagent reservoirs and suction tube. The 
tubing is not caught in the handle and seems to be fine. I have created a short 
(5min) automatic run with 5 minutes per station (no samples) and this problem 
repeats itself.



Has anybody had this problem?



Thanks for any help!

Mike


--

Message: 2
Date: Fri, 6 Jun 2014 13:53:07 -0400
From: Shirley A. Powell powell...@mercer.edu
Subject: RE: [Histonet] Processor malfunction - tissues not submerged
~ 9 hours
To: O'Donnell, Bill billodonn...@catholichealth.net, Conway,
Carla  cmcon...@usgs.gov, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
9BF995BC0E47744E9673A41486E24EE25BF92F5E81@MERCERMAIL.MercerU.local
Content-Type: text/plain; charset=utf-8

Oh where is the like button.  :)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 06, 2014 12:22 PM
To: Conway, Carla; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Not since 1978. (Sorry - couldn't resist - Happy Friday!)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Friday, June 06, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone,

When it is working correctly, our tissue processor moves a basket of tissues 
through 10 reagent containers. Last night it malfunctioned and suspended the 
tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 
hours (!) until I placed them into 70% ethanol this morning. I will process 
them next week. Has this happened to anyone else and what tissue artifacts can 
I expect?

Thanks very much,

Carla





Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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[Histonet] Processor malfunction - tissues not submerged ~ 9 hours

[Conway, Carla]

Hello everyone,

When it is working correctly, our tissue processor moves a basket of
tissues through 10 reagent containers. Last night it malfunctioned and
suspended the tissues above the 80% ethanol container. The tissues were
high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this
morning. I will process them next week. Has this happened to anyone else
and what tissue artifacts can I expect?

Thanks very much,

Carla





Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

[Grantham, Andrea L - (algranth)]

Carla,
I don't think you can be a real histotech if something like this has never 
happened to you! 
Have you figured out the cause of the malfunction?
There are recipes that you can find online and probably on HISTONET for 
solutions to soak your tissues in to rehydrate them. You might want to try a 
lesser strength alcohol and move up to 70% before processing. Good luck. 
Hopefully the tissues won't turn out to be too difficult to section. If they 
are soak them in a little water with glycerin to soften them before cutting.

Andi
96 days and counting until that retirement day!




From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Conway, Carla 
[cmcon...@usgs.gov]
Sent: Friday, June 06, 2014 7:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone,

When it is working correctly, our tissue processor moves a basket of
tissues through 10 reagent containers. Last night it malfunctioned and
suspended the tissues above the 80% ethanol container. The tissues were
high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this
morning. I will process them next week. Has this happened to anyone else
and what tissue artifacts can I expect?

Thanks very much,

Carla





Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

[O'Donnell, Bill]

Not since 1978. (Sorry - couldn't resist - Happy Friday!)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Friday, June 06, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone,

When it is working correctly, our tissue processor moves a basket of tissues 
through 10 reagent containers. Last night it malfunctioned and suspended the 
tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 
hours (!) until I placed them into 70% ethanol this morning. I will process 
them next week. Has this happened to anyone else and what tissue artifacts can 
I expect?

Thanks very much,

Carla





Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

[Shirley A. Powell]

Oh where is the like button.  :)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 06, 2014 12:22 PM
To: Conway, Carla; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Not since 1978. (Sorry - couldn't resist - Happy Friday!)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Friday, June 06, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone,

When it is working correctly, our tissue processor moves a basket of tissues 
through 10 reagent containers. Last night it malfunctioned and suspended the 
tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 
hours (!) until I placed them into 70% ethanol this morning. I will process 
them next week. Has this happened to anyone else and what tissue artifacts can 
I expect?

Thanks very much,

Carla





Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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Re: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

[jsjurczak]

Yet another reason to go microwave. This can't happen. 

- Original Message -

From: Shirley A. Powell powell...@mercer.edu 
To: Bill O'Donnell billodonn...@catholichealth.net, Carla Conway 
cmcon...@usgs.gov, histonet@lists.utsouthwestern.edu 
Sent: Friday, June 6, 2014 12:53:07 PM 
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Oh where is the like button. :) 


-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill 
Sent: Friday, June 06, 2014 12:22 PM 
To: Conway, Carla; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Not since 1978. (Sorry - couldn't resist - Happy Friday!) 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla 
Sent: Friday, June 06, 2014 9:15 AM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Hello everyone, 

When it is working correctly, our tissue processor moves a basket of tissues 
through 10 reagent containers. Last night it malfunctioned and suspended the 
tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 
hours (!) until I placed them into 70% ethanol this morning. I will process 
them next week. Has this happened to anyone else and what tissue artifacts can 
I expect? 

Thanks very much, 

Carla 





Carla Conway 
Histology Technician 
Western Fisheries Research Center, USGS 
6505 N.E. 65th Street 
Seattle, WA 98115-5016 USA 
Phone: 206-526-2042 
Fax: 206-526-6654 
E-mail: cmcon...@usgs.gov 
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Re: [Histonet] Processor problem

[Rene J Buesa]

My advise is to call Sakura and schedule a maintenance visit. The rotary valve 
may be acting up.
René J. 


On Friday, June 6, 2014 1:53 PM, Mike Tighe mti...@trudeauinstitute.org wrote:
  


I am having a problem with our tissue processor (Tissue Tek 3000). During 
processing the first reagent (NBF) gets pumped into the retort without any 
problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 
70%) however, gets about half way through pumping into retort and stops. After 
that it reverses and pumps out reagent three, then pumps in half of reagent 2. 
Pumps out the half of reagent 2 and pumps in half of reagent 1. This is 
followed by a low fluid warning.



I have removed and reinserted the reagent reservoirs and suction tube. The 
tubing is not caught in the handle and seems to be fine. I have created a short 
(5min) automatic run with 5 minutes per station (no samples) and this problem 
repeats itself.



Has anybody had this problem?



Thanks for any help!

Mike
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RE: [Histonet] Processor problem

[Denice Stiner]

I do not have a Tissue Tek 3000 but it sounds like maybe there is a sensor that 
needs wiped clean.  Clearly it's not ready the fluid level in station 3 and 
returning it back to 1 for safe keeping.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, June 06, 2014 1:56 PM
To: Mike Tighe; histonet@lists.utsouthwestern.edu 
(histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Processor problem

My advise is to call Sakura and schedule a maintenance visit. The rotary valve 
may be acting up.
René J. 


On Friday, June 6, 2014 1:53 PM, Mike Tighe mti...@trudeauinstitute.org wrote:
  


I am having a problem with our tissue processor (Tissue Tek 3000). During 
processing the first reagent (NBF) gets pumped into the retort without any 
problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 
70%) however, gets about half way through pumping into retort and stops. After 
that it reverses and pumps out reagent three, then pumps in half of reagent 2. 
Pumps out the half of reagent 2 and pumps in half of reagent 1. This is 
followed by a low fluid warning.



I have removed and reinserted the reagent reservoirs and suction tube. The 
tubing is not caught in the handle and seems to be fine. I have created a short 
(5min) automatic run with 5 minutes per station (no samples) and this problem 
repeats itself.



Has anybody had this problem?



Thanks for any help!

Mike
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[Histonet] Processor problems

[H R]

I posted the other day about having problems with my two processors. My
newest processor I believe is having an issue with
dehydration/infiltration. I have tried everything I know, plus some
suggestions from this list. I downloaded their manual for the processor and
the suggested program for this processor is 1-formalin, 3-95% alcohol,
3-100% alcohols, 3-xylenes and 3 paraffins. Does that not seem like ALOT of
alchol? And starting with 95 right after formalin seems it would lead to
problems with the salts from formalin. I've never seen a set up like above,
but I guess since my problems are with dehydration, I may try it. It just
seems like it would dry it out horribly.
-- 
*Heather*
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[Histonet] Processor question - alcoholic formalin vs. buffered formalin

[Lake, Kim S]

I attended the Tri-State Symposium in Dubuque, IA last week (which was 
fantastic) and sat in on a Boot Camp for Histology workshop.  At this 
workshop I realized that some problems we have been having with sectioning 
could be due to our processor set up.  

Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect 
that this is because we are over dehydrating our tissues in the during 
processing.  Our first solution, which the cassettes sit in for about 8 hours 
until the overnight processing begins, is alcoholic formalin, which is 450ml 
buffered formalin, 900ml water, and 3300ml 95% alcohol.  Our processor is a 
Leica TP 1050 that has been chugging along for about 20 years now.

The reason we use alcoholic formalin instead of just normal buffered formalin 
has been lost to the mists of time, and I was wondering what you all use as a 
holding solution on your processor, and why.

Thanks!

Kim Lake
Laboratory Manager
Oral Pathology Laboratory
University of Iowa College of Dentistry
Phone (319) 384 4433
Fax (319) 353 5569

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RE: [Histonet] Processor question - alcoholic formalin vs. buffered formalin

[Hannen, Valerie]

  We use 10 %  Neutral Buffered Formalin. It is a wonderful fixative and gives 
us really great fixation.
It does sound like you are overly dehydrating your tissues.

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S
Sent: Tuesday, April 30, 2013 11:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor question - alcoholic formalin vs. buffered 
formalin

I attended the Tri-State Symposium in Dubuque, IA last week (which was 
fantastic) and sat in on a Boot Camp for Histology workshop.  At this 
workshop I realized that some problems we have been having with sectioning 
could be due to our processor set up.  

Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect 
that this is because we are over dehydrating our tissues in the during 
processing.  Our first solution, which the cassettes sit in for about 8 hours 
until the overnight processing begins, is alcoholic formalin, which is 450ml 
buffered formalin, 900ml water, and 3300ml 95% alcohol.  Our processor is a 
Leica TP 1050 that has been chugging along for about 20 years now.

The reason we use alcoholic formalin instead of just normal buffered formalin 
has been lost to the mists of time, and I was wondering what you all use as a 
holding solution on your processor, and why.

Thanks!

Kim Lake
Laboratory Manager
Oral Pathology Laboratory
University of Iowa College of Dentistry
Phone (319) 384 4433
Fax (319) 353 5569

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[Histonet] Processor dehydration cycles..

[Tom McNemar]

Hello all,

I was wondering what most people use as the first reagent after the formalins 
on their tissue processor?  We have always used a sequence of 70%, 80%, 95%, 
and 100% but is anyone using 80% or even 95% to start their dehydration?

Thanks in advance.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org


This e-mail, including attachments, is intended for the sole use of the 
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recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
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Re: [Histonet] Processor dehydration cycles..

[Rene J Buesa]

I usually started with 80%EthOL but it does not harm starting with 70%EthOL 
and it is even better, from the theoretically view point. So if you have the 
space in your protocol, keep the 70%EthOL
René J.

From: Tom McNemar tmcne...@lmhealth.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Thursday, February 7, 2013 10:14 AM
Subject: [Histonet] Processor dehydration cycles..

Hello all,

I was wondering what most people use as the first reagent after the formalins 
on their tissue processor?  We have always used a sequence of 70%, 80%, 95%, 
and 100% but is anyone using 80% or even 95% to start their dehydration?

Thanks in advance.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org


This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
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[Histonet] Processor dehydration cycles

[Tim Wheelock]

Hi Tom:

I deal exclusively with post-mortum brain tissue, so my situation may 
not apply to you.
I do not use formalin on my processor, since the half brain used for 
brain-cutting has already been thoroughly fixed.
So, I have the luxury of using 30%, 50%, 80%, 95%, then three 100% 
Isopropanols.

They used this protocol when I was at a different neuropathology laboratory.
I believe the rational for this was that starting with a lower 
concentration of alcohol, and then more gradually increasing the 
concentrations, would reduce the concentration gradient between the 
cells and the solution, and so avoid strong currents from harming the cells.

Also, brain tissue may be more delicate that say prostate, skin or uterus.
By the way, I actually do not know whether this rational is correct or 
not.

However, in general, if you can start at 70%, it can't do any harm.

Tim

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA
(617) 855-359


Tom McNemar wrote:
 Hello all,

 I was wondering what most people use as the first reagent after the 
formalins on their tissue processor?  We have always used a sequence of 
70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start 
their dehydration?


 Thanks in advance.

 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
 
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org 



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[Histonet] Processor dehydration cycles

[Parker, Helayne]

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Hello all,

I was wondering what most people use as the first reagent after the 
formalins on their tissue processor?  We have always used a sequence of 70%, 
80%, 95%, and 100% but is anyone using 80% or even 95% to start their 
dehydration?

We use start at 95%, but we have two Pen Fix (alcohol fixative) steps between 
our Formalin and alcohols.  10% NBF, 10% NBF, PenFix, PenFix and then the 
alcohols starting at 95%.  

Speaking of 10% NBF has anyone ever used un-buffered formalin for routine 
processing?  I heard of a place using un-buffered formalin and wondered if 1) 
would that be okay or is it harsh on tissue and 2) would it keep the salt 
deposits down?  


Helayne Parker, H.T. (ASCP)
Pathology Section Head
Cox Medical Center Branson
P.O. Box 650, Branson, MO 65615
Phone:  417-335-7254
Fax:  417-335-7127
Email:  hpar...@skaggs.net
Web:  www.coxhealth.com/branson

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[Histonet] Processor Opinions pls

[Sheila Adey]

Hello:We are looking for a new tissue processor. I've only ever used Sakura 
products but the technology of the Leica ASP6025 looks like very good 
technology.Has anyone used this processor? If so, can you give me your 
opinion?Thanks :)Sheila  
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Re: [Histonet] Processor Opinions pls

[Kim Tournear]

Hi Sheila,
I used to install these for Leica. It's a very good machine and processes tx 
nicely. I've put surgicals and bx's on the same run (8hr) during demo's and was 
amazed at the results. I definitely recommend you do a demo. 
Hope this helps. 

Sent from the iPhone of Kim Tournear  

On Aug 9, 2012, at 4:34 PM, Sheila Adey sa...@hotmail.ca wrote:

 
 Hello:We are looking for a new tissue processor. I've only ever used Sakura 
 products but the technology of the Leica ASP6025 looks like very good 
 technology.Has anyone used this processor? If so, can you give me your 
 opinion?Thanks :)Sheila 
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Sent from the iPhone of Kim Tournear  

On Aug 9, 2012, at 4:34 PM, Sheila Adey sa...@hotmail.ca wrote:

 
 Hello:We are looking for a new tissue processor. I've only ever used Sakura 
 products but the technology of the Leica ASP6025 looks like very good 
 technology.Has anyone used this processor? If so, can you give me your 
 opinion?Thanks :)Sheila 
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[Histonet] processor solution rotation

[Steve McClain]

May I suggest?
Define a standard that works for you, combining both elements,
blocks and runs e.g.,
Every week or 200 blocks then check the first reagent  volume and quality.
Again at 400
And again at 600 blocks.
We change the first reagent whenever it appears dirty.

Consider adding a few ccs. of eosin as a visual indicator in the first 100% 
alcohol to monitor carryover and number of runs.
When the indicator reaches the first xylene, time to rotate or change.
NOTE Eosin fluoresces.

The actual blocks and runs number varies
on specimen size
and fixation, (unfixed tissues use up the alcohols)
and also whether you use sponges (more carryover)
and whether the processor drains/pumps out completely-carryover.
And on how fast the processing cycle is- short cycles demand fresh reagents and 
leave little room for error.

When in doubt do not hesitate to replenish the last solution of each type, 
alcohol, xylene and paraffin or change all solutions.
Steve
Steve A. McClain, MD
McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000

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[Histonet] Processor Preferences??

[John Baker]

Hello Jennifer,  I saw your message about tissue processors on the Histonet 
Archive May 2006 and wondered which unit you decided to purchase?  We are 
looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s 
and the Tissue-Tek VIP6.  Your thoughts on your choice and on these listed.  
Thanks you,  John

John A. Baker
The University of Michigan 
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635

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Re: [Histonet] Processor Preferences??

[Rene J Buesa]

Of the 3 you mention, I would buy VIP6. Let's see what Jennifer answers.
René J.

--- On Thu, 3/15/12, John Baker bak...@umich.edu wrote:


From: John Baker bak...@umich.edu
Subject: [Histonet] Processor Preferences??
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 15, 2012, 10:13 AM


Hello Jennifer,  I saw your message about tissue processors on the Histonet 
Archive May 2006 and wondered which unit you decided to purchase?  We are 
looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s 
and the Tissue-Tek VIP6.  Your thoughts on your choice and on these listed.  
Thanks you,  John

John A. Baker
The University of Michigan 
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635

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RE: [Histonet] Processor Preferences??

[Bernice Frederick]

If it was me and it is in the future, I want a  Leica Peloris. Leica also has a 
new one that is a single chamber version of the Peloris.
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Baker
Sent: Thursday, March 15, 2012 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor Preferences??

Hello Jennifer,  I saw your message about tissue processors on the Histonet 
Archive May 2006 and wondered which unit you decided to purchase?  We are 
looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s 
and the Tissue-Tek VIP6.  Your thoughts on your choice and on these listed.  
Thanks you,  John

John A. Baker
The University of Michigan 
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635

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Re: [Histonet] Processor Preferences??

[Jack Ratliff]

John,

Go for the ASP300S and then let's talk again at your convenience about resin 
infiltration needs. I hope the information I sent to you was both informative 
and helpful!

Regards,

Jack




On Mar 15, 2012, at 9:13 AM, John Baker bak...@umich.edu wrote:

 Hello Jennifer,  I saw your message about tissue processors on the Histonet 
 Archive May 2006 and wondered which unit you decided to purchase?  We are 
 looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s 
 and the Tissue-Tek VIP6.  Your thoughts on your choice and on these listed.  
 Thanks you,  John
 
 John A. Baker
 The University of Michigan 
 Orthopaedic Research Laboratories
 Histology Unit
 109 Zina Pitcher Place, 2218 BSRB
 Ann Arbor, MI 48109-2200
 734-936-1635
 
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[Histonet] Processor Question

[Gauch, Vicki]

Hi everyone,
We are in the market for new processors...and I was wondering if anyone could 
give me some pros and cons for the Tissue Tek VIP 6 tissue processor - how 
reliable are they? Ease of use ? Any known problems?  Tissues process well?   
You knowall the usual questions we all ask for new equipment.

Thanks in advance for your help,

Vicki Gauch
AMCH
Albany, NY



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RE: [Histonet] Processor Question

[Davide Costanzo]

Design flaw in the screen display. It is in the way of the chamber when
opening chamber. If your not careful you will break the screen. Happens
fairly often.

Sent from my Windows Phone
From: Gauch, Vicki
Sent: 2/21/2012 9:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor Question
Hi everyone,
We are in the market for new processors...and I was wondering if
anyone could give me some pros and cons for the Tissue Tek VIP 6
tissue processor - how reliable are they? Ease of use ? Any known
problems?  Tissues process well?   You knowall the usual questions
we all ask for new equipment.

Thanks in advance for your help,

Vicki Gauch
AMCH
Albany, NY



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Re: [Histonet] Processor Recomendations?

[Rene J Buesa]

I used to work with 3 VIPs, one of which was 15 years old. I really doubt that 
any of your VIPs could go at any time.
I suggest that the money you have invest in refurbishing your VIPs, a capital 
maintenance that the people from Sakura can do.
I think you should go this way before investing in another instrument.
René J.

--- On Tue, 1/31/12, Elizabeth Cameron elizabeth.came...@jax.org wrote:


From: Elizabeth Cameron elizabeth.came...@jax.org
Subject: [Histonet] Processor Recomendations?
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Tuesday, January 31, 2012, 2:57 PM


Hi,
I am looking for a reasonably-priced tissue processor for a research facility.  
We only process mouse tissue (100-200/day), and rapid processing is not 
something that is necessary.  If possible, we would like the option of using 
isopropyl alcohol for processing.  We currently have 2 Sakura VIPs that we are 
happy with, however, they are old and could go at any time.  Any 
suggestions/recommendations would be appreciated.
Thank you!

Elizabeth M. Cameron, HT, QIHC (ASCP)
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Re: [Histonet] Processor Recomendations?

[Grantham, Andrea L - (algranth)]

Stick with the VIP's - either your old ones or a refurbed newer model or even a 
NEW one if you have the money. I waited all my life for a VIP and I finally got 
one about 6 years ago and I just love it. I do animal tissue and have several 
different types of programs on the VIP (different embryo stages, different 
animal species brains, plants, insects, and routine too!)
It has never failed and produces excellent processed tissues.


Andi Grantham




On Jan 31, 2012, at 1:40 PM, Helen Fedor wrote:

 Where is the LIKE button?
 
 Helen
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
 Sent: Tuesday, January 31, 2012 3:31 PM
 To: histonet@lists.utsouthwestern.edu; Elizabeth Cameron
 Subject: Re: [Histonet] Processor Recomendations?
 
 I used to work with 3 VIPs, one of which was 15 years old. I really doubt 
 that any of your VIPs could go at any time.
 I suggest that the money you have invest in refurbishing your VIPs, a capital 
 maintenance that the people from Sakura can do.
 I think you should go this way before investing in another instrument.
 René J.
 
 --- On Tue, 1/31/12, Elizabeth Cameron elizabeth.came...@jax.org wrote:
 
 
 From: Elizabeth Cameron elizabeth.came...@jax.org
 Subject: [Histonet] Processor Recomendations?
 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
 Date: Tuesday, January 31, 2012, 2:57 PM
 
 
 Hi,
 I am looking for a reasonably-priced tissue processor for a research 
 facility.  We only process mouse tissue (100-200/day), and rapid processing 
 is not something that is necessary.  If possible, we would like the option of 
 using isopropyl alcohol for processing.  We currently have 2 Sakura VIPs that 
 we are happy with, however, they are old and could go at any time.  Any 
 suggestions/recommendations would be appreciated.
 Thank you!
 
 Elizabeth M. Cameron, HT, QIHC (ASCP)
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Re: [Histonet] Processor Recomendations?

[Michele Email]

I agree VIP's are the way to go!
Michele Carr

Sent from my iPad

On Jan 31, 2012, at 3:06 PM, Grantham, Andrea L - (algranth) 
algra...@email.arizona.edu wrote:

 Stick with the VIP's - either your old ones or a refurbed newer model or even 
 a NEW one if you have the money. I waited all my life for a VIP and I finally 
 got one about 6 years ago and I just love it. I do animal tissue and have 
 several different types of programs on the VIP (different embryo stages, 
 different animal species brains, plants, insects, and routine too!)
 It has never failed and produces excellent processed tissues.
 
 
 Andi Grantham
 
 
 
 
 On Jan 31, 2012, at 1:40 PM, Helen Fedor wrote:
 
 Where is the LIKE button?
 
 Helen
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
 Sent: Tuesday, January 31, 2012 3:31 PM
 To: histonet@lists.utsouthwestern.edu; Elizabeth Cameron
 Subject: Re: [Histonet] Processor Recomendations?
 
 I used to work with 3 VIPs, one of which was 15 years old. I really doubt 
 that any of your VIPs could go at any time.
 I suggest that the money you have invest in refurbishing your VIPs, a 
 capital maintenance that the people from Sakura can do.
 I think you should go this way before investing in another instrument.
 René J.
 
 --- On Tue, 1/31/12, Elizabeth Cameron elizabeth.came...@jax.org wrote:
 
 
 From: Elizabeth Cameron elizabeth.came...@jax.org
 Subject: [Histonet] Processor Recomendations?
 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
 Date: Tuesday, January 31, 2012, 2:57 PM
 
 
 Hi,
 I am looking for a reasonably-priced tissue processor for a research 
 facility.  We only process mouse tissue (100-200/day), and rapid processing 
 is not something that is necessary.  If possible, we would like the option 
 of using isopropyl alcohol for processing.  We currently have 2 Sakura VIPs 
 that we are happy with, however, they are old and could go at any time.  Any 
 suggestions/recommendations would be appreciated.
 Thank you!
 
 Elizabeth M. Cameron, HT, QIHC (ASCP)
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[Histonet] Processor Help

[Freeman, Lisa*]

If anyone has a Shandon Excelsior ES and can offer me some help, I would 
greatly appreciate it. I have one and have used it very little as it was the 
back up processor. My old work horse, Pathcentre, is down and now the Excelsior 
ES will be my main processor. I have zero training and zero instruction on how 
to operate this processor.
Anyone willing to answer a few questions, please let me know.
Thank you,


Lisa Freeman, HT
Histology Supervisor
Toxicologic Pathology Associates
National Center for Toxicological Research
3900 NCTR RD
Jefferson AR 72079
Phone: 870-543-7234
E-mail: lisa.free...@fda.hhs.gov



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[Histonet] processor stopped - help!

[Daniela Bodemer]

Hi all,

 

When I arrived the lab this morning I found the processor basket with my
cassettes stuck between the two paraffin tanks. I suppose it went
through the first cycle but not the second one, so its missing 1.5 hrs
of paraffin infusion. Should I put them back in hot paraffin for 1.5
hours or what can I do to save the samples?

 

Thanks for your advice,

 

Daniela Bodemer 

Research Assistant

Surgical Research, Infection and Immunity

 

Murdoch Childrens Research Institute

The Royal Children's Hospital

Flemington Road Parkville Victoria 3052 Australia 

T 03 9345 5930 T (03 9345 4116) 

E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au 

www.mcri.edu.au http://www.mcri.edu.au/ 

 

This e-mail and any attachments to it (the Communication) are, unless
otherwise stated, confidential, may contain copyright material and is
for the use only of the intended recipient. If you receive the
Communication in error, please notify the sender immediately by return
e-mail, delete the Communication and the return e-mail, and do not read,
copy, retransmit or otherwise deal with it. Any views expressed in the
Communication are those of the individual sender only, unless expressly
stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21
006 566 972 or any of its related entities. MCRI does not accept
liability in connection with the integrity of or errors in the
Communication, computer virus, data corruption, interference or delay
arising from or in respect of the Communication. 

P  Please consider the environment before printing this email

 


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RE: [Histonet] processor stopped - help!

[Daniela Bodemer]

I am glad to hear the samples will be fine.

 

Thanks everyone!

 

Daniela Bodemer 

Research Assistant

Surgical Research, Infection and Immunity

 

Murdoch Childrens Research Institute

The Royal Children's Hospital

Flemington Road Parkville Victoria 3052 Australia 

T 03 9345 5930 T (03 9345 4116) 

E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au 

www.mcri.edu.au http://www.mcri.edu.au/ 

 

This e-mail and any attachments to it (the Communication) are, unless
otherwise stated, confidential, may contain copyright material and is
for the use only of the intended recipient. If you receive the
Communication in error, please notify the sender immediately by return
e-mail, delete the Communication and the return e-mail, and do not read,
copy, retransmit or otherwise deal with it. Any views expressed in the
Communication are those of the individual sender only, unless expressly
stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21
006 566 972 or any of its related entities. MCRI does not accept
liability in connection with the integrity of or errors in the
Communication, computer virus, data corruption, interference or delay
arising from or in respect of the Communication. 

P  Please consider the environment before printing this email

 


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[Histonet] Processor power supply Back-up

[kristen arvidson]

Does anyone have any suggestions on power supply back-up for Leica ASP 300?  We 
are an independant lab and are not hooked up to back-up if we have an outage.  
Thought it might be a good idea :)
 
Thanks.
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[Histonet] Processor change

[sgoebel]

How many blocks do you guys normally process before you change the
solutions in the processor?

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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RE: [Histonet] Processor change

[Breeden, Sara]

I change solutions after 500 blocks; paraffin after 1500 blocks.

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RE: [Histonet] Processor change

[jstaruk]

Change or rotate?

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 www.masshistology.com
   www.nehorselabs.com
 
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Monday, November 29, 2010 4:18 PM
To: sgoe...@mirnarx.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor change

I change solutions after 500 blocks; paraffin after 1500 blocks.

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-
No virus found in this message.
Checked by AVG - www.avg.com
Version: 10.0.1170 / Virus Database: 426/3286 - Release Date: 11/28/10


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Re: [Histonet] Processor change

[Rene J Buesa]

When the processor capacity is reached, I start to rotate the reagents, 
discarding the first of each group, moving forward the remaining, and replacing 
with fresh reagents the last.
René J.

--- On Mon, 11/29/10, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote:


From: sgoe...@mirnarx.com sgoe...@mirnarx.com
Subject: [Histonet] Processor change
To: histonet@lists.utsouthwestern.edu
Date: Monday, November 29, 2010, 4:04 PM


How many blocks do you guys normally process before you change the
solutions in the processor?



Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912



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RE: [Histonet] Processor change

[Laurie Colbert]

Nancy Klemme, can you comment on this specifically for the Tissue Tek
VIP's?  Does Sakura have guidelines for rotating the solutions?
Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Monday, November 29, 2010 1:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor change

How many blocks do you guys normally process before you change the
solutions in the processor?

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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[Histonet] Processor needed

[Mauger, Joanne]

Hi All,
We are in need of a used tissue processor. If anyone has one for sale in the 
Philadelphia area, please email ros...@email.chop.edu. Budget is very small!!!

Thank you.

Joanne Mauger HT(ASCP)QIHC
Children's Hospital of Philadelphia

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - 
SMMC-SF [karen.heckf...@chw.edu]
Sent: Monday, April 19, 2010 7:57 AM
To: Michelle MacVeigh-Aloni
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Does anyone have and use Shandon Excelsior EX

I have had one for a few years now and love it.  You can also monitor it
from another location if you have analog internet.   It is really easy
to change out the reagents and is way less messy than most other
processors.  You need to make sure your reagents stay topped off.  The
only real problems I have had with it is a Pathologist loading it wrong
and not setting it down on the tongs correctly and breaking my basket
that the cassettes set into.
That was kind of a nightmare.  I had to retrain the Pathologist.
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle
MacVeigh-Aloni
Sent: Friday, April 16, 2010 12:51 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Does anyone have and use Shandon Excelsior EX

Hi all,

According to the advertisement, this is supposed to be the most
histotech friendly processor. Does anyone have a comment?

Michelle
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[Histonet] Processor malfunction

[Charles, Roger]

Happy Friday to all,
Our processor shut off last evening in 80% alcohol due to a misconnection of 
the next reagent. We restarted this morning when we arrived but the tissues 
were sitting in 80% for 9 hours.  My question is what affect will this have on 
the tissues which are a mixture of medulla and lymph node sections?
Thanks
roger

Roger Charles
Microbiologist II
PA Veterinary Laboratory
717-787-8808

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Re: [Histonet] processor

[Piero Nelva]

I agree. THe Peloris is a robust processor that handles all our routine 
overnight and 2 hour cycles easily.  Also love the reagent managment system 
which assesses reagent purity.  It does a good job on our skin specimens.


Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia


- Original Message - 
From: Kim Tournear kimtourn...@yahoo.com

To: Histonet histonet@lists.utsouthwestern.edu
Sent: Tuesday, December 30, 2008 5:15 AM
Subject: Re: [Histonet] processor


Hi Jane,
We just bought 2 Peloris processors from Leica. We can process in as little 
as 1 hour (usually biopsy specimens). Each machine has 2 processing chambers 
and can process up to 300 blocks per chamber. It was a good choice for us 
since we do appx 15,000 cases a year...Maintenance is easyI use the 
Pathos from Leica (microwave technology) at my part time job processing 
dermatology specimens (appx 10,000 cases a year) and it works great. Contact 
Leica for some brochures on their equipment.

Hope this helps...

~Kim Tournear ~
Histology Supervisor
Tucson Medical Center
Tucson, AZ

~Don't let your life end before it begins~

OU Rocks

--- On Mon, 12/29/08, Jane C. Moose jane.mo...@newberryhospital.net wrote:

From: Jane C. Moose jane.mo...@newberryhospital.net
Subject: [Histonet] processor
To: histonet@lists.utsouthwestern.edu
Date: Monday, December 29, 2008, 7:10 AM

I know this question repeatedly occurs,  but our tissue processor
died
last week.  We are a small hospital lab  doing about 2500 cases a year
(6000 specimens.)  Would anyone have a recommendation on a new
processor for our size lab?  An Excelsior was brought in for demo (not
sure what model)  but that one was being discontinued and we were
waiting to see the new model. We were also waiting on a new  model
VIP
to bring in for demo-but that has not happened yet.   Any advice would
be greatly appreciated.  Thanks Jane



Jane Moose

LIS Coordinator

Newberry County Memorial Hospital

Newberry, SC  29108

P-803-405-7129

F- 803-405-7474



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[Histonet] processor

[Jane C. Moose]

I know this question repeatedly occurs,  but our tissue processor died
last week.  We are a small hospital lab  doing about 2500 cases a year
(6000 specimens.)  Would anyone have a recommendation on a new
processor for our size lab?  An Excelsior was brought in for demo (not
sure what model)  but that one was being discontinued and we were
waiting to see the new model. We were also waiting on a new  model VIP
to bring in for demo-but that has not happened yet.   Any advice would
be greatly appreciated.  Thanks Jane

 

Jane Moose

LIS Coordinator

Newberry County Memorial Hospital

Newberry, SC  29108

P-803-405-7129

F- 803-405-7474

 

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Re: [Histonet] processor

[Kim Tournear]

Hi Jane,
We just bought 2 Peloris processors from Leica. We can process in as little as 
1 hour (usually biopsy specimens). Each machine has 2 processing chambers and 
can process up to 300 blocks per chamber. It was a good choice for us since we 
do appx 15,000 cases a year...Maintenance is easyI use the Pathos from 
Leica (microwave technology) at my part time job processing dermatology 
specimens (appx 10,000 cases a year) and it works great. Contact Leica for some 
brochures on their equipment. 
Hope this helps...
 
~Kim  Tournear ~
Histology Supervisor
Tucson Medical Center
Tucson, AZ 
 
~Don't let your life end before it begins~
 
OU Rocks

--- On Mon, 12/29/08, Jane C. Moose jane.mo...@newberryhospital.net wrote:

From: Jane C. Moose jane.mo...@newberryhospital.net
Subject: [Histonet] processor
To: histonet@lists.utsouthwestern.edu
Date: Monday, December 29, 2008, 7:10 AM

I know this question repeatedly occurs,  but our tissue processor
died
last week.  We are a small hospital lab  doing about 2500 cases a year
(6000 specimens.)  Would anyone have a recommendation on a new
processor for our size lab?  An Excelsior was brought in for demo (not
sure what model)  but that one was being discontinued and we were
waiting to see the new model. We were also waiting on a new  model
VIP
to bring in for demo-but that has not happened yet.   Any advice would
be greatly appreciated.  Thanks Jane

 

Jane Moose

LIS Coordinator

Newberry County Memorial Hospital

Newberry, SC  29108

P-803-405-7129

F- 803-405-7474

 

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