Hi Jim,
You mention that the OCT seems a little softer.
Could it be that the sections are thicker than you usually produce. Check the
Frozen section HE to check this.
If sections are thicker they will tend to detach from the slides.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Wednesday, 3 September 2014 12:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen sections for Immunoflouresence
Lately we are experiencing an issue where the tissue sections are coming off of
the slide during the staining process. We have always used positive charged
slides and have not experienced this problem on a routine case. We do not fix
the slides but allow them to air dry for Immunology. Some have suggested a
quick fixation in acetone or 95% ETOH. This doesn't seem to correct the
problem and we don't find the IF stains work as well. We have tried a
different batch of slides and are looking into a different kind of coating for
the slides. Our regular frozen sections stay on the slides. We are using
OCT. The only other thing I have noticed is that maybe it's my imagination
but the OCT in the block surrounding the kidney biopsy seems a little softer
than normal. I did try a different mounting medium and maybe the softness was
my imagination. Has anyone else experienced this problem and if so how did
you correct it? The lab is air conditioned so I don't think the humidity is
the main factor. The Immunology staff changed all of their reagents so we have
reduced that variable hopefully. We are using Leica positive charged slides.
(I have found that the Apex control box slides from Leica are a little better
than the regular Apex slides. Any ideas?
Jim
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