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Ng et al Re-hydration Method for Red Blood Cell Lysis
Principle:
R-ehydration of air dried smears with normal saline for 30 seconds
before fixation in alcohol has been found to produce slides of a quality
superior or at least equivalent to that of immediate fixed smears. It
allowed the cells to adhere better to the slide and lysis of red blood
cells provided a clean background for better assessment.
Solutions:
1. Normal Saline
Sodium Chloride 9g
Distilled water 1000ml
2. 95% Ethanol
Procedure:
1. After smearing material, air dry slides using a hair dryer.
2. Place slides in Normal Saline for 30sec.
3. Immediately place slides in 95% ethanol and fix for 10 minutes.
4. Stain slides via PAP.
Results:
RBCs were effectively lysed but epithelial and mesothelial cells were
retained. In general, smears showed a decrease in chromaticity of both
nuclear and cytoplasmic staining. Cytoplasmic vacuoles were less
distinct and epithelial fragments, such as acini, appeared flattened and
could be more easily focussed on the same plane. Nuclear and cellular
moulding was exaggerated and more appreciable when present.
There was a slight enlargement of cells. Degenerated specimens, even in
the absence of polymorphs, often resulted in disappointing re-hydrated
smears. Cell clusters were easier to see probably because of the higher
transparency of the cytoplasm.
Reference:
Acta Cytolog (1994) 38(1):56-64.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath
Raj
Sent: Thursday, 12 August 2010 10:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 31
Dear Friends,
Does anyone have rehydration technique of air dried smear
which can be stained for pap.
Aazathraj.P
Technical Officer
Department of Histopathology and Cytology
Apollo Hospitals -chennai
India
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