I assume you mean what reagent to use as the negative control? The ideal
control is a mixture of negative mouse serum and negative rabbit specifically
titered to match the antibody concentration in the cocktail, which is probably
proprietary. The next best solution is to use a universal negative control
serum. The universal component typically denotes use with rabbit and mouse
antibodies.
Will Chappell, HTL(ASCP)QIHC
Histologist at large
Sent from my iPhone
On Feb 21, 2011, at 8:07 AM, Carol Bryant cb...@lexclin.com wrote:
I would like that information also.
Thank you,
Carol
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
Sent: Sunday, February 20, 2011 3:00 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Pin4cocktail negative controls
Hello Histonetters,
What are you folks using as a negative control when running Pin4 cocktail
antibody.
Thanks in advance,
MG
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